Splenectomy for wandering spleen with pedicle torsion in a 17-year-old: a case report.

Background: Wandering spleen is a rare disorder characterized by splenic displacement into the abdominal or pelvic cavity, predisposing to torsion and infarction. Congenital factors (long pedicle, lax ligaments) or acquired conditions (splenomegaly) are the usual causes.

Case Presentation: A 17-year-old female presented with 5 days of escalating abdominal pain.

Plumbagin inhibits CYP2J/EETs metabolic pathway and leads to heart injury in cardiac organoids and rats.

Cytochrome P450 2J (CYP2J) enzymes are critical in synthesizing cardioprotective epoxyeicosatrienoic acids (EETs), which help reduce oxidative stress, inflammation, and apoptosis. Plumbagin, a natural antitumor agent, induces cardiac injury, although its mechanism remains uncharacterized. This study systematically evaluated the mechanism behind plumbagin-induced heart injury.

The multifaceted impact of physical exercise on FoxO signaling pathways.

This review explores the multifaceted impact of physical exercise on FoxO signaling pathways, which play a central role in cellular homeostasis, stress response, metabolism, and longevity. Exercise influences FoxO proteins-particularly FoxO1, FoxO3, FoxO4, and FoxO6-through diverse mechanisms, including phosphorylation, acetylation, and ubiquitination, determining their localization, transcriptional activity, and stability. Regular exercise modulates FoxO signaling by activating pathways like PI3K/AKT, AMPK, SIRT1, and IGF-1, promoting cellular resilience against oxidative stress, apoptosis, and metabolic dysfunction.

CYP3A mediates drug-drug interactions between atorvastatin and omeprazole: Evidence from in vitro and in vivo studies.

The simultaneous use of statins and proton pump inhibitors is common in clinical practice, but the mechanisms behind their potential drug-drug interactions (DDIs) are still not fully understood. This study investigates the cytochrome P450 3A (CYP3A)-mediated interactions between atorvastatin and omeprazole through in vitro microsomal assays and in vivo pharmacokinetic studies in wild-type (WT) and Cyp3a1/2 knockout (KO) rats. Incubation experiments with rat and human liver microsomes demonstrated that omeprazole inhibited atorvastatin metabolism in a concentration-dependent manner.

Gut derived (S)-Equol mitigates influenza viral pneumonia by modulating macrophage polarization via Nrf2 mediated AKT/ERK/NF-κb signaling pathways.

Respiratory virus including influenza A virus (IAV) infection induces alterations in gut microbiota structure and function, which in turn plays an essential role in the pathogenic process. Alterations in gut microbiota are usually accompanied with changes in metabolites. The specific relationship between dynamic changes in gut microbiota and serum metabolites in influenza remains unclear.

Impact of Needle-Tip Depth on Cement Leakage in Percutaneous Vertebroplasty: A Clinical and Finite Element Analysis.

Objective: The purpose of this study was to explore the optimal needle-tip depth through conventional percutaneous vertebroplasty (PVP) and further analyze the correlation between needle-tip depth and anterior cement leakage in osteoporotic vertebral compression fractures.

Methods: A total of 560 patients with PVP were retrospectively analyzed, and they were stratified into a shallow (needle-tip at 2/3-3/4 depth, n = 291) and deep placement group (3/4-7/8 depth, n = 269). The clinical outcomes and anterior leakage rates were compared.

An emerging role of mitochondrial quality control in bone metabolism: from molecular mechanisms to targeted therapeutic interventions.

The mitochondrial quality control system is the principal regulatory framework governing mitochondrial quantity, morphology, distribution, and functional integrity. This surveillance and regulatory machinery is essential for preserving cellular homeostasis and determining cellular differentiation. Mitochondria play a central role in maintaining the dynamic equilibrium between osteogenic differentiation and osteoclastic differentiation.

Constructing a Preeclampsia Organoid Model to Elucidate the Mechanism of Aspirin.

Background: Preeclampsia is a life-threatening pregnancy disorder characterized by hypertension and multiorgan dysfunction, posing significant risks to both maternal and fetal health. Although low-dose aspirin is widely recommended for preventing preeclampsia, the underlying mechanisms of action are still poorly understood, which hinders the optimization of therapeutic strategies.

Methods: We developed an in vitro hypoxia-induced preeclampsia model using human trophoblast organoids to replicate key pathological features.

Enhanced Lethal Effects of Combined P-tert-Butylcatechol and L-Lysine on .

Allelochemicals are recognized as promising algaecides due to their environmental safety. Para-tert-butylcatechol (TBC) and L-lysine exhibit significant potential in suppressing harmful algal blooms (HABs); however, their combined effects and algae inhibition mechanisms remain unelucidated. Therefore, this study systematically investigated the growth inhibition of by TBC and L-lysine individually and in combination, while simultaneously examining their combined effects on algal growth, cell membrane integrity, photosynthetic activity, antioxidant responses, and microcystin production.

Metabolomic profiling of plasma reveals differential disease severity markers in avian influenza A(H7N9) infection patients.

Objectives: Avian influenza such as H7N9 is currently a major global public health risk, and at present, there is a lack of relevant diagnostic and treatment markers.

Methods: We collected plasma samples from 104 confirmed H7N9 patients, 31 of whom died. Plasma metabolites were detected by UHPLC-HRMS, and a survival prediction model based on metabolites was constructed by machine-learning models.

Single-microbe RNA sequencing uncovers unexplored specialized metabolic functions of keystone species in the human gut.

The human body is inhabited by trillions of microorganisms that play a crucial role in health and diseases. Our understanding of the species and functional composition of the human gut microbiome is rapidly expanding, but it is still mainly based on taxonomic profiles or gene abundance measurements. As such, little is known about the species-function heterogeneity and dynamic activities in human microecosystem niches.

mKmer: an unbiased K-mer embedding of microbiomic single-microbe RNA sequencing data.

The advanced single-microbe RNA sequencing (smRNA-seq) technique addresses the pressing need to understand the complexity and diversity of microbial communities, as well as the distinct microbial states defined by different gene expression profiles. Current analyses of smRNA-seq data heavily rely on the integrity of reference genomes within the queried microbiota. However, establishing a comprehensive collection of microbial reference genomes or gene sets remains a significant challenge for most real-world microbial ecosystems.

The impact of implemented in China on the public's subjective well-being-an empirical analysis based on CGSS2021.

Background: Communities are the main places where the public spend their daily activities, and suitable community environments can help improve their subjective well-being. However, there is still limited evidence on how fitness environments in communities affect the public's subjective well-being. In recent years, Chinese governments have been created the in communities to provide the public with convenient fitness facilities and venues.

The effect of temperature on the photosynthetic carbon fixation efficiency of sessile macroalgae in the mussel farming area of Gouqi Island through stable isotope.

This study aimed to investigate the carbon fixation capacity of macroalgae in the mussel farming area of Gouqi Island and to quantify further the carbon fixation effect during the cultivation process of macroalgae. Using high-abundance NaCO as a tracer combined with stable isotope technology, we quantitatively analyzed the photosynthetic carbon fixation rate, biological carbon sequestration amount, and particulate organic carbon generation rate of three macroalgae species-Ulva pertusa, Sargassum horneri, and Grateloupia turuturu-across a range of temperatures (5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C). The results showed that Ulva pertusa exhibited the highest photosynthetic carbon fixation rate and carbon sequestration amount, peaking at 25 °C and 20 °C, respectively.

ESPN activates ZEB1-mediated EMT through the PI3K/AKT/mTOR axis to promote osteosarcoma metastasis.

Background: Osteosarcoma (OS) is a primary bone malignancy characterized by early metastasis and generally poor prognosis. ESPN is highly expressed and plays an important role in regulating the aggressive phenotypes of several cancer cell types. However, little is known about the molecular mechanisms underlying ESPN-mediated migration and invasion in OS cells.

Establishment of Novel Simple Sequence Repeat Markers in and Application in Wetlands of Nanhui Dongtan, Shanghai.

Common reed () is a widely distributed perennial herb of high ecological significance in wetlands worldwide. This research developed 15 SSR markers through whole-genome sequencing and assessed the genetic diversity across four representative reed populations (DB, JPDP, YJRHK, DD) in the coastal wetlands of Nanhui Dongtan, Shanghai. A total of 113.

Fast Encapsulation of Microbes into Dissolvable Hydrogel Beads Enables High-Throughput Microbial Single-Cell RNA Sequencing of Clinical Microbiome Samples.

Microbial single-cell RNA-seq (mscRNA-seq) can achieve resolution at the cellular level, enhancing the understanding of microbial communities. However, current high-throughput mscRNA-seq methods are limited by multiple centrifugation steps, which can lead to microbial loss and bias. smGel-seq is reported, a high-throughput single-microbe RNA sequencing method for clinical microbiome samples that employs hydrogel beads to encapsulate individual microbes to reduce microbial loss and input requirements.

Identifying risk factors associated with the health-related quality of life for coronary heart diseases elderly using association rule mining.

Health-related quality of life (HRQoL) is a crucial outcome measure in the care of elderly patients with coronary heart disease (CHD). This research aims to identify risk factors associated with HRQoL in elderly patients with CHD. A cross-sectional study was carried out in Shanghai, China, from January to May 2023.

Betacyanin accumulation mediates photosynthetic protection in Suaeda salsa (L.) Pall. under salt stress.

Soil salinization threatens sustainable agriculture, necessitating innovative restoration strategies. Suaeda salsa (L.) Pall.

Comorbidity patterns and implications for disease control: a network analysis of medical records from Shanghai, China.

Background: The aging problem in Shanghai is rapidly increasing, leading to the development of chronic comorbidities in older adults. Studying the correlations within comorbidity patterns can assist in managing disease prevention and implicate early control.

Objectives: This study was a cross-sectional analysis based on a large sample size of 3,779,756 medical records.

Driving green digital innovation in higher education: the influence of leadership and dynamic capabilities on cultivating a green digital mindset and knowledge sharing for sustainable practices.

Purpose: The new generation loves to interact with digital technologies. On the other hand, due to the soaring digital innovation trends, sustainability is crucial in higher educational institutes (HEI), necessitating investigating the factors that lead to green innovations. Therefore, drawing on the Stimulus-organism-response approach (SOR) and dynamic capability view, the current study examines the intricate relationship between digital leadership, dynamic capability, knowledge sharing, green digital mindset and green digital innovation in higher education settings.

Theoretical investigation of an arsenene/g-CN van der Waals heterojunction: a direct Z-scheme system with high photocatalytic efficiency.

With advancements in algorithms and computational power, theoretical calculations have become increasingly feasible for designing and constructing functional materials. In this study, we utilized density functional theory (DFT) to investigate the new arsenene/g-CN van der Waals heterojunction, which forms a direct Z-scheme system with an indirect bandgap of 1.41 eV and a minimal lattice mismatch of just 1.

Carboxylesterase 2A gene knockout or enzyme inhibition alleviates steatohepatitis in rats by regulating PPARγ and endoplasmic reticulum stress.

Metabolic dysfunction associated steatotic liver disease (MASLD) is a widespread liver disease that progresses from simple steatosis to severe steatohepatitis stage. Despite the recognized importance of carboxylesterase 2 (CES2) in hepatic lipid metabolism, the role of CES2 in hepatic inflammation remains unclear. The rat genome encodes six Ces2 genes and Ces2a shows high expression in the liver and intestine.

Clinical Characteristics and Predicting Disease Severity in Chlamydia psittaci Infection Based on Metagenomic Next-Generation Sequencing.

Introduction: Psittacosis pneumonia, as a zoonotic infection, is induced by the pathogen . In the present study, we sought to characterize the clinical manifestations and prognosticate the severity of psittacosis pneumonia.

Methods: We retrospectively verified instances of psittacosis pneumonia in Zhejiang province, China, from January 2021 to April 2024.

The impact of internet use on physical activity among Chinese older adults: the mediating role of social support.

Background: The positive impact of Internet use on enhancing physical activity among older adults is an important response to the aging problem in the new era. However, while previous studies have explored the important impact of Internet use on physical activity among older adults, the specific mechanisms of this impact have not been much analyzed. In an attempt to answer this question, we further focus on the mediating role of social support in the relationship between Internet use and physical activity among older adults.