Efficient Production of a Novel Subtilisin-like Serine Protease in for the Preparation of DPP-IV Inhibitory Activity Peptides from Protein Byproducts.

Proteases play a crucial role in the bioconversion of proteins into bioactive peptides. is an important cell factory for enzyme production due to its strong post-translational modification capabilities and excellent protein secretion system. In this study, a novel subtilisin-like serine protease (S8) from was efficiently expressed extracellularly in FBL-B for the first time using a polycistronic system and the coexpression strategy of the gene (hemoglobin from ).

High-level secretory expression and characterization of an acid protease in Komagataella phaffii and its application in soybean meal protein degradation.

Acid proteases play a crucial role in the industrial enzyme market, but low yield limits their widespread application. In this study, we focused on enhancing the secretory expression level of an acid protease (AopepA) from Aspergillus oryzae in Komagataella phaffii through stepwise genetic modification strategies. These included the co-expression of endoplasmic reticulum secretion-associated factors, overexpression of eukaryotic translation initiation factors, knockout of the β-1,3-glucanosyltransferase gene, disruption of the hypoxic heme-dependent repressor gene, and co-expression of the hemoglobin gene.

Characterization of a novel aspartic protease from Trichoderma asperellum for the preparation of duck blood peptides.

A novel aspartic protease gene (TaproA1) from Trichoderma asperellum was successfully expressed in Komagataella phaffii (Pichia pastoris). TaproA1 showed 52.8% amino acid sequence identity with the aspartic protease PEP3 from Coccidioides posadasii C735.

Development of a two-enzyme system in Aspergillus niger for efficient production of N-acetyl-β-D-glucosamine from powdery chitin.

A chitinase (PbChi70) from Paenibacillus barengoltzii was engineered by directed evolution to enhance its hydrolysis efficiency towards powder chitin. Through two rounds of screening, a mutant (mPbChi70) with a maximum specific activity of 73.21 U/mg was obtained, which is by far the highest value ever reported.

CRISPR/Cas9 System-Mediated Multi-copy Expression of an Alkaline Serine Protease in for the Production of XOD-Inhibitory Peptides.

CRISPR/Cas9 system-mediated multi-copy expression of an alkaline serine protease (S8) from was successfully built in . Furthermore, AoproS8 was continuously knocked in the , , and gene loci in to construct multi-copy expression strains. The yield of the AoproS8 3.

Deconstructive Synthesis of Bridged and Fused Rings via Transition-Metal-Catalyzed "Cut-and-Sew" Reactions of Benzocyclobutenones and Cyclobutanones.

Bridged and fused rings are commonly found in biologically important molecules. Current tactics to construct these ring systems are primarily based on stepwise ring formation (i.e.

Catalytic Activation of Unstrained C(Aryl)-C(Alkyl) Bonds in 2,2'-Methylenediphenols.

Catalytic activation of unstrained and nonpolar C-C bonds remains a largely unmet challenge. Here, we describe our detailed efforts in developing a rhodium-catalyzed hydrogenolysis of unstrained C(aryl)-C(alkyl) bonds in 2,2'-methylenediphenols aided by removable directing groups. Good yields of the monophenol products are obtained with tolerating a wide range of functional groups.

Characterization of a Novel Aspartic Protease from Expressed in and Its Application in Production of ACE-Inhibitory Peptides.

is an important fungus that produces aspartic proteases suitable for cheese processing. In this study, a novel aspartic protease gene () was cloned from CAU432 and expressed in . The amino acid sequence of RmproB shared the highest identity of 58.

Total Synthesis of Penicibilaenes via C-C Activation-Enabled Skeleton Deconstruction and Desaturation Relay-Mediated C-H Functionalization.

Herein, we describe the first total synthesis of sesquiterpene penicibilaenes A and B through a "C-C/C-H" approach. In the "C-C" stage, the Rh-catalyzed "cut-and-sew" transformation between trisubstituted alkene and cyclobutanone has been employed to construct the unique tricyclo[6.3.

Synthesis of 17-Deacetoxyl Chromodorolide B Based on a Gold-Catalyzed Alkoxycyclization Reaction.

A novel strategy to construct the highly oxidized 3-oxabicyclo[3.3.0]octane skeleton was developed via a gold-catalyzed cascade cyclization with 2,7-dioxabicyclo[3.

The antibiotic activity and mechanisms of active metabolites (Streptomyces alboflavus TD-1) against Ralstonia solanacearum.

Objectives: In order to elucidate the antibacterial activity and mechanism of S. alboflavus TD-1 active metabolites, the minimal inhibitory concentration of R. solanacearum and other effects on cell wall, cell membrane, nucleic acid, protein and cell morphology were studied.

The Antioxidation of Different Fractions of Dill (Anethum graveolens) and Their Influences on Cytokines in Macrophages RAW264.7.

Dill (Anethum graveolens L.) has been shown strong antioxidative and immune propertise, but the precise potency and action mechanisms remain largely elusive. This study is to dissect the different fractions' antioxidant power and antiinflammatory function.

Enantioselective synthesis of alkaloids and vinblastine rearrangements of quaternary ammoniums.

An efficient and novel strategy for the enantioselective syntheses of various alkaloids has been developed. The salient features include a gold-catalyzed oxidation of a terminal alkyne followed by cyclization, a Stevens rearrangement and a tandem sequence that combines the gold-catalyzed oxidation, cyclization and [1,2]-shift. The catharanthine analogs provided by our approach were further converted to the alkaloid vinblastine and its analogs, which confirmed the remarkable sensitivity of the cytotoxicity to the C20' substituent of vinblastine.