The effect of U2AF1 mutation on erythroid differentiation and sensitivity to demethylation drug treatment.

Myelodysplastic syndrome (MDS) is a group of blood disorders characterized by impaired maturation of erythroid cells. Mutations in the U2 small nuclear RNA auxiliary factor 1 (U2AF1) gene, particularly S34 (S34F/Y) and Q157 (Q157P/R), have been identified in 5-10% of MDS patients. By analyzing U2AF1 expression in hematopoietic stem progenitor cells and different blood cells in the GEO database, we have found the expression pattern of U2AF1 showed a significant stage specificity during normal erythropoiesis.

Seeking Common Ground While Reserving Differences: Multiple Anatomy Collaborative Framework for Undersampled MRI Reconstruction.

Recently, deep neural networks have greatly advanced undersampled Magnetic Resonance Image (MRI) reconstruction, wherein most studies follow the one-anatomy-one-network fashion, i.e., each expert network is trained and evaluated for a specific anatomy.

Microflow LC-MS/MS reveals platform-specific post-translational modification signatures in recombinant adeno-associated virus capsids linked to enhanced potency and stability: A quality control framework for gene therapy.

Recombinant adeno-associated viruses (rAAVs) are pivotal gene therapy vectors due to their safety and stable transduction, yet comprehensive characterization of capsid post-translational modifications (PTMs)-critical for potency, immunogenicity, and manufacturing consistency-remains limited across production platforms. This study employs microflow LC-MS/MS coupled with electron-activated dissociation (EAD) to analyze PTMs in clinically relevant rAAV5 and rAAV9 serotypes produced via mammalian (HEK293) and insect (Sf9) cells, with parallel cellular-level evaluation of vector potency and infectivity, conducted under matched purity and capsid thermal stability conditions to isolate PTM-specific effects. Intact mass analysis revealed conserved N-terminal acetylation in VP1/VP3 across both platforms, while PTM profiling identified six distinct modification types, including deamidation, oxidation, and phosphorylation, with Sf9-derived vectors exhibiting 14 % more PTMs than HEK293-produced counterparts.

Development of a multi-indicator risk prediction model for cervical cancer associated with benzo[a]pyrene and nicotine exposure: A multi-omics study integrating toxicological analyses and molecular docking.

Background: Exposure to the tobacco-related compounds Benzo[a]pyrene and Nicotine has been associated with the development of several diseases. The aim of this study was to investigate the common genes associated with cervical cancer, construct a risk prediction model to reveal their biological functions, and evaluate the prognostic significance of the model to identify its potential value in the treatment of cervical cancer.

Methods: In this study, genes associated with Benzo[a]pyrene and Nicotine and cervical cancer-related genes were screened by multiple databases.

Activity and Resistance Assessment of Three Acetohydroxyacid Synthase Inhibitors on Development, Pathogenicity, and Inhibitor-Target Interaction Analysis.

The branched-chain amino acid biosynthesis pathway was predicted to be one of the essential pathways in the plant pathogen species. In this study, we evaluated the activity of nine herbicides targeting acetohydroxyacid synthase (AHAS) in this pathway against . Three herbicides, iodosulfuron-methyl, chlorimuron-ethyl, and florasulam, exhibited inhibitory effects on mycelial growth, with EC values of 1.

Root-specific CsATs enhances acylated flavonol-glycoside accumulation to promote aluminum tolerance in tea roots.

Acylated flavonol glycosides can regulate plant growth, improve plant stress resistance and disease resistance. We have found that some specific acetylated flavonol glycosides accumulated in the tea roots, but their metabolic characteristics and the mechanism of biosynthetic regulation were not clear. In this paper, the flavonol glycosides in tea seedlings were qualitatively and quantitatively analyzed by UPLC-TOF-MS/MS and UPLC-QqQ-MS/MS.

FLAG3D++: A Benchmark for 3D Fitness Activity Comprehension With Language Instruction.

Recent years have witnessed the rapid development of general human action understanding. However, when applied to real-world applications such as sports analysis, most existing datasets are still unsatisfactory, because of the limitations in rich labels on multiple tasks, language instructions, high-quality 3D data, and diverse environments. In this paper, we present FLAG3D++, a large-scale benchmark for 3D fitness activity comprehension, which contains 180 K sequences of 60 activity categories with language instruction.

Chromosome-level genome assembly of the ornamental plant Alcea rosea.

Alcea rosea, a member of the Malvaceae family, is celebrated for its rich floral palette and global horticultural significance. Here, we present a high-quality reference genome for A. rosea, achieving a genome assembly size of 1.

Improving the color quality evaluation of holographic prints with light pillars.

In order to study the influence of color difference contributed by different lightness, chroma, and hue differences on the human visual perception, 84 pairs of holographic prints from nine cigarette packaging products within 3.0 CIELAB color difference units were prepared. A color difference evaluation experiment was carried out by 22 experienced observers.

Segment Concealed Objects With Incomplete Supervision.

Incompletely-Supervised Concealed Object Segmentation (ISCOS) involves segmenting objects that seamlessly blend into their surrounding environments, utilizing incompletely annotated data, such as weak and semi-annotations, for model training. This task remains highly challenging due to (1) the limited supervision provided by the incompletely annotated training data, and (2) the difficulty of distinguishing concealed objects from the background, which arises from the intrinsic similarities in concealed scenarios. In this paper, we introduce the first unified method for ISCOS to address these challenges.

CreativeSynth: Cross-Art-Attention for Artistic Image Synthesis With Multimodal Diffusion.

Although remarkable progress has been made in image style transfer, style is just one of the components of artistic paintings. Directly transferring extracted style features to natural images often results in outputs with obvious synthetic traces. This is because key painting attributes including layout, perspective, shape, and semantics often cannot be conveyed and expressed through style transfer.

Urinary exosomes exacerbate diabetic kidney disease by promoting NLRP3 inflammasome activation via the microRNA-516b-5p/SIRT3/AMPK pathway.

Diabetic kidney disease (DKD) is a severe complication of diabetes mellitus. Urinary exosomal miRNAs play a prominent regulatory role in the pathogenesis of DKD, but the potential mechanisms remain largely unknown. Our research was designed to explain the pathogenesis of urine-derived exosomal microRNA-516b-5p (miR-516b-5p) in the DKD development.

Investigation of the attenuation effect of licorice on the toxicity of rhubarb using a P-gp lipid raft bioaffinity chromatography.

Ethnopharmacological Relevance: As a classic clinical drug pair that was first recorded in Shanghan Lun, the combination of rhubarb and licorice has been known to reduce the toxic side effects of rhubarb, while enhancing its therapeutic efficacy. Despite its benefits, the exact mechanism through which licorice mitigates toxicity requires further investigation.

Aim Of The Study: This research seeks to explore the effect of main active components of licorice on absorption and transport of rhein and emodin as well as provide new insights into the detoxification properties of licorice.

Diagnostic Captioning by Cooperative Task Interactions and Sample-Graph Consistency.

Radiographic images are similar to each other, making it challenging for diagnostic captioning to narrate fine-grained visual differences of clinical importance. In this paper, we propose a self-boosting framework integrating two novel strategies to learn tightly correlated image and text features for diagnostic captioning. The first strategy explicitly aligns image and text features through training an auxiliary task of image-text matching (ITM) jointly with the main task of report generation (RG) as two branches of a network model.

Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection.

Jiawei Ermiao Granule (JWEMG) is a traditional Chinese herbal formulation widely used in China for the treatment of human papilloma virus (HPV) infections. However, the mechanisms underlying their efficacy in clearing HPV infections remain unclear. This study aimed to elucidate the mechanisms by which JWEMG clears persistent HPV infections from a metabolomics perspective using modern analytical techniques.

Immunogenic cell death genes in single-cell and transcriptome analyses perspectives from a prognostic model of cervical cancer.

Background: The role of immunogenic cell death (ICD) in cervical cancer (CESC) is not well understood. This study sought to investigate the significance of ICD in CESC and to establish an ICDRs prognostic model to improve immunotherapy efficacy for patients with cervical cancer.

Methods: ICD-associated genes were screened at the single-cell and transcriptome levels based on AddModuleScore, single-sample gene set enrichment analysis (ssGSEA) and weighted gene co-expression network (WGCNA) analysis.

Isolation, purification and structural characterization of wheat-derived beer protein.

Wheat beer is known for its rich and long-lasting foam, which is largely attributed to the proteins derived from wheat. In this study, a wheat-derived protein was isolated and purified from pure wheat malt wort based on foam properties and structurally characterized. The molecular weight of this protein is 15 kDa and its foaming and foam stability were 64.

Multi-Omics Analysis Revealed That TAOK1 Can Be Used as a Prognostic Marker and Target in a Variety of Tumors, Especially in Cervical Cancer.

Background: Thousand and One Kinase 1 (TAOK1), a member of the MAPK kinase family, plays a crucial role in processes like microtubule dynamics, DNA damage response, and neurodevelopment. While TAOK1 is linked to tumorigenesis, its oncogenic role across cancers remains unclear. This study aims to explore the relationship between TAOK1 expression, prognosis, and immune function in various cancers.

Metformin-mediated intestinal AMPK activation ameliorates PCOS through gut microbiota modulation and metabolic pathways.

Introduction: Polycystic ovary syndrome (PCOS) is a complex disorder characterized by metabolic and ovulatory dysfunctions, often associated with an imbalance in gut microbiota. Despite current treatments, effective management strategies targeting underlying mechanisms remain limited.

Methods: In this study, we used a rat model of PCOS induced by letrozole and a high-fat diet.

Diffusion Models in Low-Level Vision: A Survey.

Deep generative models have gained considerable attention in low-level vision tasks due to their powerful generative capabilities. Among these, diffusion model-based approaches, which employ a forward diffusion process to degrade an image and a reverse denoising process for image generation, have become particularly prominent for producing high-quality, diverse samples with intricate texture details. Despite their widespread success in low-level vision, there remains a lack of a comprehensive, insightful survey that synthesizes and organizes the advances in diffusion model-based techniques.

LETSmix: a spatially informed and learning-based domain adaptation method for cell-type deconvolution in spatial transcriptomics.

Spatial transcriptomics (ST) enables the study of gene expression in spatial context, but many ST technologies face challenges due to limited resolution, leading to cell mixtures at each spot. We present LETSmix to deconvolve cell types by integrating spatial correlations through a tailored LETS filter, which leverages layer annotations, expression similarities, image texture features, and spatial coordinates to refine ST data. Additionally, LETSmix employs a mixup-augmented domain adaptation strategy to address discrepancies between ST and reference single-cell RNA sequencing data.

Tailoring the Mechanical Properties of Polymer/Nanorod Nanocomposites through Polymer Functionalization.

The rational design of polymers to improve nanorod dispersion and strengthen polymer-nanorod interfacial interactions is crucial for designing nanorod-filled polymer nanocomposites (PNCs). Herein, using coarse-grained molecular dynamics simulations, we studied the effect of polymer chain functionalization on the dispersion state of nanorods, the diffusion/relaxation of polymer matrix chains, and the mechanical properties of the corresponding PNCs. The simulation results showed that the nanorod dispersion state could be adjusted by functionalizing the polymer chain.

Circulating microRNAs and physical activity: Impact in diabetes.

The term "ci-miRNAs," or "circulating microRNAs," refers to extracellular microRNAs (miRNAs) that exist outside of cells and can be detected in various bodily fluids, including blood, saliva, urine, and breast milk. These ci-miRNAs play a role in regulating gene expression and are mainly recognized for their functions beyond the cell, serving as signaling molecules in the blood. Researchers have thoroughly investigated the roles of these circulating miRNAs in various diseases.

Clinical features and prognostic predictors of C. Psittaci Pneumonia: a systematic review and individual patient meta-analysis.

Background: The clinical presentations and prognostic indicators of C. psittaci pneumonia are inadequately investigated currently. The objective of the study was to assess the clinical presentation of C.

Jiawei Ermiao Granules (JWEMGs) clear persistent HR-HPV infection though improving vaginal microecology.

Ethnopharmacological Relevance: Jiawei Ermiao Granules (JWEMGs), a traditional Chinese herbal formulation, has been widely used in China for the treatment of human papillomavirus (HPV) infections. However, the underlying mechanisms through which it exerts its antiviral effects remain poorly understood.

Aim Of The Study: This study aimed to investigate the potential mechanisms by which JWEMGs modulate vaginal microecology and clear HPV infections, utilizing clinical trials, metagenomic sequencing, and in vitro models.