Modified pea apyrase has altered nuclear functions and enhances the growth of yeast and Arabidopsis.

Apyrases (NTPDases) regulate growth and development in multiple eukaryotic organisms and function in multiple sub-cellular locales. An earlier report showed that the ectopic expression of psNTP9 (PS), a chromatin-associated pea () apyrase, enhanced the uptake of inorganic phosphate (Pi) and increased the growth of yeast and Arabidopsis. In this follow-up study, we generated a modified form of PS, abbreviated DM ("double mutant"), in which two-point mutations, S208L and P216R, were introduced into its DNA-binding domain.

[Effectiveness of three-dimensional-printed microporous titanium prostheses combined with flap implantation in treatment of large segmental infectious bone defects in limbs].

Objective: To analyze the effectiveness of single three-dimensional (3D)-printed microporous titanium prostheses and flap combined prostheses implantation in the treatment of large segmental infectious bone defects in limbs.

Methods: A retrospective analysis was conducted on the clinical data of 76 patients with large segmental infectious bone defects in limbs who were treated between January 2019 and February 2024 and met the selection criteria. Among them, 51 were male and 25 were female, with an age of (47.

A Neural Network Model for Intelligent Classification of Distal Radius Fractures Using Statistical Shape Model Extraction Features.

Objective: Distal radius fractures account for 12%-17% of all fractures, with accurate classification being crucial for proper treatment planning. Studies have shown that in emergency settings, the misdiagnosis rate of hand/wrist fractures can reach up to 29%, particularly among non-specialist physicians due to a high workload and limited experience. While existing AI methods can detect fractures, they typically require large training datasets and are limited to fracture detection without type classification.

PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.

The phytochrome (phy) family of sensory photoreceptors modulates developmental programs in response to ambient light. Phys also control gene expression in part by directly interacting with the bHLH class of transcription factors, PHYTOCHROME-INTERACTING FACTORS (PIFs), and inducing their rapid phosphorylation and degradation. Several kinases have been shown to phosphorylate PIFs and promote their degradation.

[Three-dimensional finite element model construction and biomechanical analysis of customized titanium alloy lunate prosthesis].

Objective: To design customized titanium alloy lunate prosthesis, construct three-dimensional finite element model of wrist joint before and after replacement by finite element analysis, and observe the biomechanical changes of wrist joint after replacement, providing biomechanical basis for clinical application of prosthesis.

Methods: One fresh frozen human forearm was collected, and the maximum range of motions in flexion, extension, ulnar deviation, and radialis deviation tested by cortex motion capture system were 48.42°, 38.

Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism.

Osteonecrosis of the femoral head (ONFH) is a common disabling disease. Copper has positive effects on cells that regulate bone metabolism. However, the relationship between copper metabolism (CM) and steroid-induced ONFH (SONFH) remains unclear.

Metacarpal Lengthening Using a Mini-Ring Ilizarov Device for Thumb Reconstruction.

Purpose: To explore the results of using the mini-ring Ilizarov external fixator for thumb metacarpal lengthening and its compatibility with a simultaneous groin flap.

Methods: From May 2016 to June 2019, 17 adult patients with thumb loss were treated with metacarpal lengthening using a mini-ring Ilizarov device. The device was composed of 2 rings, threaded rods, nuts, and K-wires (diameter, 1.

[Research progress in etiology and clinical diagnosis and treatment of scaphotrapeziotrapezoid osteoarthritis].

Objective: To summarize the research progress of scaphotrapeziotrapezoid osteoarthritis (STT OA) and its etiology and clinical treatment.

Methods: The domestic and foreign literature on STT OA in recent years was reviewed and the research progress was summarized.

Results: STT OA is a common OA, which is highly prevalent in postmenopausal women and diagnosed by wrist X-ray films.

ERECTA regulates seed size independently of its intracellular domain via MAPK-DA1-UBP15 signaling.

Seed size is determined by the coordinated growth of the embryo, endosperm, and integument. Growth of the integument is initiated by signal molecules released from the developing endosperm or embryo. Although recent studies have identified many components that regulate seed size by controlling integument growth, the upstream signals and the signal transduction pathway that activate these components after double fertilization are unclear.

Feasibility of cortical bone trajectory screws for bridging fixation in revision surgery for lumbar adjacent segment degeneration.

Background: To investigate the feasibility of using cortical bone trajectory (CBT) screws for bridging fixation in revision surgery for lumbar adjacent segment degeneration and to provide a reference for clinical practice.

Methods: Computed tomography scans of the lumbar spines of 36 patients in our hospital were used. Sixteen males and 20 females with an average age of 65.

Clinical Application of 3-Dimensional Printed Navigation Templates in Treating Femoral Head Osteonecrosis With Pedicled Iliac Bone Graft.

Objective: The aim of the study was to explore the feasibility and early effect of digital design combined with 3-dimensional (3D) printing technique in the transplantation of vascular pedicled iliac bone flap in the treatment of avascular necrosis of the femoral head.

Methods: The navigation template was designed according to computed tomography scan and printed in 3D printing technique before operation, which was used to guide the localization and clearance of osteonecrosis of the femoral head in vascular pedicled iliac bone flap transplantation. In blank control group, 28 cases (32 hips) of osteonecrosis of the femoral head were treated with vascular pedicled iliac bone flap without the assistance of 3D navigation template from February 2002 to February 2009, including 19 males (21 hips) and 9 females (11 hips), with an average age of 37 years (range, 20-61 years).

[Research progress of artificial wrist joint prosthesis].

Objective: To summarize the research progress of the artificial wrist joint prosthesis.

Methods: Domestic and abroad literature concerning artificial wrist joint prosthesis was reviewed and analyzed thoroughly.

Results: Artificial wrist joint prosthesis has been developed to the 4th generation.

The Brassinosteroid-Activated BRI1 Receptor Kinase Is Switched off by Dephosphorylation Mediated by Cytoplasm-Localized PP2A B' Subunits.

Brassinosteroid (BR) binding activates the receptor kinase BRI1 by inducing heterodimerization with its co-receptor kinase BAK1; however, the mechanisms that reversibly inactivate BRI1 remain unclear. Here we show that cytoplasm-localized protein phosphatase 2A (PP2A) B' regulatory subunits interact with BRI1 to mediate its dephosphorylation and inactivation. Loss-of-function and overexpression experiments showed that a group of PP2A B' regulatory subunits, represented by B'η, negatively regulate BR signaling by decreasing BRI1 phosphorylation.