Molecular imaging of stem cell therapies in ischemic stroke.

Ischemic stroke is a leading cause of mortality and morbidity worldwide, lacking effective approaches to restore neurological functions in clinical practice. Stem cell therapies for ischemic stroke show great potential in neural regeneration, neuroprotection, microenvironment regulation, and neural network reconstruction. Stem cells including mesenchymal stem cells (MSCs), embryogenic stem cells (ESCs), induced pluripotential stem cells (iPSCs), neural stem cells (NSCs), et al.

The effects of melancholic features on the efficacy of high-frequency rTMS in adolescents and young adults with major depressive disorder.

Objective: This research examined the effects of neuronavigated high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) on adolescents and young adults with major depressive disorder (AYA-MDD) with melancholic features. It also explored neuroimaging differences between melancholic (MD) and non-melancholic (NMD) AYA-MDD subtypes.

Method: Forty-four AYA-MDD participants (14 MD, 30 NMD) underwent 10 daily HF-rTMS sessions over two weeks.

Deficient FANCL Predisposes to Endothelial Damage: A New Therapeutic Target for Pulmonary Hypertension.

Clinical observations have suggested an association between alkylating agent-based chemotherapy and pulmonary arterial hypertension (PAH). The Fanconi anemia (FA) pathway, the principal mechanism for resolving alkylating agent-induced DNA damage, has been implicated in this process. To establish the interplay among the FA pathway, DNA damage, and PAH.

Forebrain neural progenitors effectively integrate into host brain circuits and improve neural function after ischemic stroke.

Human cortical neural progenitor cell transplantation holds significant potential in cortical stroke treatment by replacing lost cortical neurons and repairing damaged brain circuits. However, commonly utilized human cortical neural progenitors are limited in yield a substantial proportion of diverse cortical neurons and require an extended period to achieve functional maturation and synaptic integration, thereby potentially diminishing the optimal therapeutic benefits of cell transplantation for cortical stroke. Here, we generated forkhead box G1 (FOXG1)-positive forebrain progenitors from human inducible pluripotent stem cells, which can differentiate into diverse and balanced cortical neurons including upper- and deep-layer excitatory and inhibitory neurons, achieving early functional maturation simultaneously in vitro.

Duplicated OsATG9 Genes Antagonise Autophagy to Balance Growth and Drought Tolerance in Rice.

Gene duplication events frequently occur during eukaryotic genome evolution, often leading to functional redundancy for organism survival in complex environments. However, whether duplicate genes evolve diverse functions remains unclear. In this study, we explored the roles of autophagy-related gene 9 OsATG9a and OsATG9b in rice development and drought stress responses.

The Difference in Mortality Rates Between Endovascular and Conservative Treatment for Patients with Chronic Limb-Threatening Ischemia: A Propensity Score Matching Study.

Background: The main objective of this study was to examine the impact of endovascular therapy versus conservative therapy on mortality rates among patients with chronic limb-threatening ischemia (CLTI). In addition, the outcomes of amputation-free survival (AFS) were also studied in both groups.

Methods: In this observational registry study, we retrospectively analyzed patients admitted to the Department of Vascular Surgery at Beijing Shijitan Hospital, Capital Medical University, from January 2016 to September 2023.

Oral administration of pioglitazone inhibits pulmonary hypertension by regulating the gut microbiome and plasma metabolome in male rats.

The oral administrated thiazolidinediones (TZDs) have been widely reported to alleviate experimental pulmonary hypertension (PH). However, previous studies mainly focused on their beneficial effects on the cardiopulmonary vascular system but failed to determine their potential roles on gut microenvironment. This study aims to investigate the effects of pioglitazone, an oral TZD drug, on gut microbiome in classic PH rat models induced by hypoxia (HPH) or SU5416/hypoxia (SuHx-PH) and evaluate the therapeutic potential of supplementation of selective probiotics for experimental PH.

Effects of Clearfil SE Bond 2 on restoring deciduous anterior teeth with ring caries and analysis of caries risk factors in children aged 3-8 years.

To investigate the impact of various self-acid corrosion adhesives in restoring ring caries in anterior deciduous teeth and the influencing factors of childhood caries. This study retrospectively analyzed the repair effect of two kinds of restoration schemes on the annular caries of children's deciduous anterior teeth. According to the repair scheme, 54 children with caries were divided into two groups.

PET imaging of synaptic vesicle glycoprotein 2 subtype A for neurological recovery in ischemic stroke.

Purpose: [F]SynVesT-1 is a novel radiopharmaceutical for assessing synaptic density in vivo. This study aims to investigate the potential of [F]SynVesT-1 positron emission tomography (PET) in evaluating neurological recovery in the rat model of ischemic stroke, and to compare its performance with [F]FDG PET.

Methods: Sprague-Dawley rats were subjected to photothrombotic cerebral infarction, and safinamide was administered intraperitoneally from day 3 to day 14 post-stroke to alleviate neurological deficits.

Variation in Rice Plastid Genomes in Wide Crossing Reveals Dynamic Nucleo-Cytoplasmic Interaction.

Plastid genomes (plastomes) of angiosperms are well known for their relative stability in size, structure, and gene content. However, little is known about their heredity and variations in wide crossing. To such an end, the plastomes of five representative rice backcross inbred lines (BILs) developed from crosses of / were analyzed.

Airway delivery of Streptococcus salivarius is sufficient to induce experimental pulmonary hypertension in rats.

Background And Purpose: The causal relationship between altered host microbiome composition, especially the respiratory tract microbiome, and the occurrence of pulmonary hypertension (PH) has not yet been studied. An increased abundance of airway streptococci is seen in patients with PH compared with healthy individuals. This study aimed to determine the causal link between elevated airway exposure to Streptococcus and PH.

The efficacy of stenting in the iliofemoral vein of patients with venous obstruction and secondary lymphedema from malignancy.

Objective: To investigate the safety and effectiveness of venous stenting in patients with chronic iliofemoral venous obstruction and secondary lymphedema from malignancy.

Methods: From July 2012 to December 2020, patients with iliofemoral venous obstruction and secondary lymphedema who underwent venous stenting in our institution were reviewed retrospectively. Clinical characteristics, surgical complications, and symptom relief were assessed.

Effect of static magnetic field treatment on γ-aminobutyric acid content and sensory characteristics of germinated brown rice cake.

This study aimed to improve γ-aminobutyric acid (GABA) content and sensory characteristics of brown rice (BR) cake by static magnetic field (SMF)-assisted germination. BR was pre-treated by SMF (10 mT, 60 min, 25 °C), germinated for 36 h, and then germinated BR (GBR) was used to prepare rice cake. The optimal formula was: 60 % GBR, 40 % white rice, 1 % yeast, 20 % sugar, and 55 % water.

Cystic Adventitial Disease of the Popliteal Vein: A Case Report.

Venous cystic adventitial disease (VCAD) is a rare vascular anomaly located in the common femoral vein in most cases. We describe the case of a 59-year-old female patient with right leg edema who was misdiagnosed with deep vein thrombosis of the lower extremity at another hospital. Magnetic resonance angiography revealed a round mass in the popliteal vein, with a narrow lumen.

Long noncoding RNA TUG1 induces angiogenesis of endothelial progenitor cells and dissolution of deep vein thrombosis.

Objective: Long non-coding RNA (lncRNA) essentially controls many physiological and pathological processes of deep vein thrombosis (DVT). Based on that, lncRNA taurine upregulated gene 1 (TUG1)-involved angiogenesis of endothelial progenitor cells (EPCs) and dissolution of DVT was explored.

Methods: In the in-vitro experiments, EPCs were engineered with mimic, inhibitor, siRNA, and plasmid, after which tube formation, proliferation, migration, and apoptosis were checked.

Upregulation of mechanosensitive channel Piezo1 involved in high shear stress-induced pulmonary hypertension.

Introduction: Piezo1 is an important mechanosensitive channel implicated in vascular remodeling. However, the role of Piezo1 in different types of vascular cells during the development of pulmonary hypertension (PH) induced by high shear stress is largely unknown.

Materials And Methods: We used a rat PH model established by left pulmonary artery ligation (LPAL, for 2-5 weeks), which mimics the high flow and hemodynamic stress, to study Piezo1 contribution to pulmonary vascular remodeling.

Visualization of Mitochondria During Embryogenesis in Zebrafish by Aggregation-Induced Emission Molecules.

Purpose: Aggregation-induced emission (AIE) molecules have been widely utilized for fluorescence imaging in many biomedical applications, benefited from large Stokes shift, high quantum yield, good biocompatibility, and resistance to photobleaching. And visualization of mitochondria is almost investigated in vitro and ex vivo, but in vivo study of mitochondria is more essential for systematic biological research, especially during embryogenesis. Therefore, suitable and time-saving alternatives with simple operation based on AIE molecules are urgently needed compared with traditional transgenic approach.

Intensifying the moderate electric field-induced modification of maize starch by 1-butyl-3-methylimidazolium chloride.

This study aimed to improve the effect of moderate electric field (MEF) treatment on the structural and physicochemical properties of maize starch by adding 1-butyl-3-methylimidazolium chloride ([bmim]Cl). Starch (20%, w/v) was mixed with [bmim]Cl solution (0, 30%, 50%, and 70%, w/w), and then electro-treated (5 V/cm, 50 Hz) at 60 °C for 10 min. As the [bmim]Cl concentration increased, the electro-induced disintegration of starch granules was enhanced due to the intensified Joule heat and decreased pH of reaction bulk.

MicroRNA-136-5p from Endothelial Progenitor Cells-released Extracellular Vesicles Mediates TXNIP to Promote the Dissolution of Deep Venous Thrombosis.

Objective: Endothelial progenitor cells-released extracellular vesicles (EPCs-EVs) have previously been reported to promote the dissolution of deep venous thrombosis (DVT) through delivery of microRNA (miR). Given that, this research was projected to search the relative action of EPCs-EVs transferring of miR-136-5p in DVT.

Methods: From EPCs transfected with miR-136-5p agomir or antagomir, EVs were extracted and then injected into DVT mice.

Positron emission tomography molecular imaging-based cancer phenotyping.

During the past several decades, numerous studies have provided insights into biological characteristics of cancer cells and identified various hallmarks of cancer acquired in the tumorigenic processes. However, it is still challenging to image these distinctive traits of cancer to facilitate the management of patients in clinical settings. The rapidly evolving field of positron emission tomography (PET) imaging has provided opportunities to investigate cancer's biological characteristics in vivo.