Automated, aseptic sampling with small-volume capacity from microbioreactors for cell therapy process analysis.

Current workflows in autologous cell therapy manufacturing are reliant on manual processes that are difficult to scale out to meet patient demands. High throughput bioreactor systems that enable multiple cultures to occur in parallel can address this need, but require good bioprocess monitoring workflows to produce good quality cell therapy products. Commercial sampling systems have thus been developed for better feedback control and monitoring capabilities.

trans-Configured Ligands Boost Spin Crossover to Room Temperature in Mononuclear Fe(II) Complexes.

Seven new mononuclear complexes [Fe(aqin)(NCS)] (1), [Fe(aqin)(NCSe)] (2), [Fe(aqin)(NCBH)] (3), [Fe(2-pic)(NCS)] (4), [Fe(2-pic)(NCSe)] (5), [Fe(2-pic)(NCBH)] (6), [Fe(tpa)(NCBH)] (7), [aqin = 8-aminoquinoline, 2-pic = 2-picolylamine, tpa = tris(2-pyridylmethyl)amine)] are investigated. Structurally, except for 3, which affords a trans-configuration, all the complexes adopt a cis-configuration. Their propensity to crystallize without any solvent molecules facilitates the study of the influence of the "pure" ligand on spin-crossover (SCO) characteristics.

Survival and Morphological Changes of Spores Co-Exposed to Antibiotics and Simulated Gastrointestinal Fluids: Implications for Antibiotic Stewardship.

Probiotics are often advised to be taken separately from antibiotics due to their sensitivity to antibiotic activity and gastrointestinal (GI) stress. However, spores, as next-generation probiotics, may withstand concurrent use with antibiotics due to their unique structural adaptations. This study systematically evaluated the survival rates and morphological changes of spores exposed to 10 clinically relevant antibiotics in simulated gastric/intestinal fluids, exploring their feasibility for co-administration with antibiotics.

Synergistic effects of PD-1 antibody and chemotherapy followed by surgery-centric local treatment in patients with limited-metastatic gastric or gastroesophageal adenocarcinoma (ROSETTE trial): an open-label, single-center, randomized phase 2 trial.

Background: Limited metastatic gastric cancer (lmGC) represents an intermediate disease stage, positioned between localized and widely disseminated gastric cancer, and has garnered increasing attention due to its distinct prognostic outcomes. Currently, there is no consensus on the optimal treatment approach for lmGC, raising the question of whether it should align more with the systemic treatment-focused approach used for metastatic gastric cancer or adopt a surgery-centric strategy similar to that used in localized disease. Previous studies have preliminarily explored combining systemic treatment and surgical resection to address both the primary tumor and metastatic lesions.

Transition from manual to automated processes for autologous T cell therapy manufacturing using bioreactor with expandable culture area.

Transition from the manual processes that are performed during the initial research and development (R&D) stage to automated processes for later and commercial stage cell therapy manufacturing can be challenging. It often requires significant effort, time, and costs - which hinders the therapy's access to the clinic. To ease this transition, we have developed a novel and flexible manufacturing platform, Bioreactor with Expandable Culture Area (BECA), that aims to support both R&D and manufacturing to accelerate cell therapies from bench to bedside.

Altered Mode of Structural Changes in Solid Solutions Leading to Dual Modulation: Spin Transition Temperatures and Steps.

The development of high-density information storage materials requires precise control of electron spin states. Multistep spin-crossover (SCO) materials with multiple stable spin states are prime candidates for this purpose. However, accurate control of the dynamic SCO behavior, including the dual dynamic modulation of the spin transition temperature () and transition steps, has been a significant hurdle.

De novo-designed amphiphilic α-helical peptide Z2 exhibits broad-spectrum antimicrobial, anti-biofilm, and anti-inflammatory efficacy in acute Pseudomonas aeruginosa pneumonia.

Antimicrobial peptides (AMPs) show considerable promise in combating bacterial infections due to their broad-spectrum efficacy, unique mechanisms of action, and resistance capabilities. In this study, we de novo designed a series of α-helical AMPs (Z1-Z6) with enhanced antimicrobial activity, anti-biofilm, and anti-inflammatory effects. The design incorporated isoleucine with long alkyl side chains and carefully balanced the positive charge and hydrophobicity.

Device for automated aseptic sampling: Automated sampling solution for future cell and gene manufacturing.

Cell sampling is a key step performed regularly throughout the cell manufacturing process to gather cell samples for cell growth, progress, and characteristics analysis. While the current method of sampling by pipetting in a biosafety cabinet is commonly used, it is labour-intensive and susceptible to contamination risks. We have developed Device for Automated Aseptic Sampling (DAAS), to enable automated, small volume (0.

Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica.

β-ionone, an apocarotenoid derived from a C40 terpenoid has an intense, woody smell and a low odor threshold that has been widely used in as an ingredient in food and cosmetics. Yarrowia lipolytica is a promising host for β-ionone production because of its oleaginous nature, its ability to produce high levels of acetyl-CoA (an important precursor for terpenoids), and the availability of synthetic biology tools to engineer the organism. In this study, β-carotene-producing Y.

Two-Dimensional Spin-Crossover Molecular Solid Solutions with Tunable Transition Temperatures across 90 K.

Spin-crossover (SCO) materials exhibit remarkable potential as bistable switches in molecular devices. However, the spin transition temperatures () of known compounds are unable to cover the entire ambient temperature spectrum, largely limiting their practical utility. This study reports an exemplary two-dimensional SCO solid solution system, = 5--2-hydroxybenzylidene-hydrazinecarbothioamide, = F or Cl, = 0 to 1), in which the adjacent layers are adhered via hydrogen bonding.

AcHZP45 is a repressor of chlorophyll biosynthesis and activator of chlorophyll degradation in kiwifruit.

The degradation of chlorophyll during fruit development is essential to reveal a more 'ripe' color that signals readiness to wild dispersers of seeds and the human consumer. Here, comparative biochemical analysis of developing fruit of Actinidia deliciosa cv. Xuxiang ('XX', green-fleshed) and Actinidia chinensis cv.

Prevalence of post-dialysis fatigue: a systematic review and meta-analysis.

Objectives: The purpose of this study was to synthesise data on the prevalence of post-dialysis fatigue (PDF) among haemodialysis (HD) patients.

Design: Systematic review and meta-analysis.

Data Sources: China National Knowledge Infrastructure, Wanfang, Chinese Biological Medical Database, PubMed, EMBASE and Web of Science were searched from their inception to 1 April 2022.

Anthraquinone metabolites isolated from the rhizosphere soil Streptomyces of Panax notoginseng (Burk.) F. H. Chen target MMP2 to inhibit cancer cell migration.

Ethnopharmacological Relevance: Panax notoginseng (Burk.) F. H.

Effect of Kangaroo Mother Care on the Psychological Stress Response and Sleep Quality of Mothers With Premature Infants in the Neonatal Intensive Care Unit.

Objective: To investigate the effect of kangaroo mother care on the psychological stress response and sleep quality of mothers with premature infants admitted to the neonatal intensive care unit (NICU).

Methods: A randomized controlled design was used to recruit participants. The study recruited 126 mothers of premature infants in the NICU from January 2019 to January 2020.

Guanxinping Tablets Inhibit ET-1-Induced Proliferation and Migration of MOVAS by Suppressing Activated PI3K/Akt/NF-B Signaling Cascade.

Background/aim: Abnormal proliferation and migration of vascular smooth muscle cells is one of the main causes of (AS). Therefore, the suppression of abnormal proliferation and migration of smooth muscle cells are the important means for the prevention and inhibition of AS. The clinical effects of Guanxinping (GXP) tablets and preliminary clinical research on the topic have proved that GXP can effectively treat coronary heart disease, but its underlying mechanism remains unclear.

Ultrastable Polyoxometalate-Encapsulated Supramolecular Metal-Organic Nanotubes for Single-Crystal Proton Conduction.

Two unique polyoxometalate (POM)-encapsulated tubular materials with the formula K(HO)[M(btp)(HO)](PWO)(Hbtp)(btp)·52HO [M = Mn () and Co (); btp = 2,6-bis(1,2,4-triazol-1-yl)pyridine] were designed and synthesized based on the Dawson POM and V-type btp ligand, as confirmed by IR, X-ray diffraction (XRD), and element analysis. Single-crystal XRD analyses of compound show that two kinds of remarkable metal-organic supramolecular nanotubes, including trigonal and hexagonal nanotubes, are constructed along the -axis direction via π···π-packing interactions between {Mn(btp)} rings and the btp ligands, of which [α-PWO] anions are confined in channels, making the entire structure extraordinarily stable. Meanwhile, the coordinated [α-PWO] anion within the hexagonal channel makes the channel highly hydrophilic and attracts a number of guest water molecules to fill in the free space, conducive to proton transport.

Sterile Fluid Transfer for Cell Therapy Manufacturing-The Value of Multiple-Use Aseptic Connector.

Sterile fluid transfer between vessels is a key step in cell therapy manufacturing and requires specialised devices to maintain sterility of both vessels before, during and after the transfer. This review introduces two main types of devices for sterile fluid transfer in cell therapy manufacturing, namely single-use sterile connectors and tube welders. While these are excellent devices for infrequent moderate to large volume transfers, a new multiple-use aseptic connector may fill the gap for frequent small volume fluid transfers that are particularly important for autologous cell therapy manufacturing.

Association between Hyperosmolar Hyperglycemic State and Venous Thromboembolism in Diabetes Patients: A Nationwide Analysis in Taiwan.

Background: Previous studies in Western countries have shown that a hyperosmolar hyperglycemic state (HHS) is associated with an increased risk of venous thromboembolism (VTE); in these cases, prophylactic anticoagulant treatment is suggested. However, the association between HHS and VTE in Asian populations remains undetermined. Therefore, we aimed to evaluate whether HHS is associated with an increased risk of VTE in diabetic Taiwanese patients.

[Clinical Analysis of Bloodstream Infection after Hematopoietic Stem Cell Transplantation].

Objective: To analyze the clinical characteristics of bloodstream infection (BSI) in patients treated by hematopoietic stem cell transplantation (HSCT).

Methods: The clinical characteristics, distribution of pathogenic bacteria causing BSI and drug sensitivity of 910 patients treated by HSCT in our department from January 2013 to June 2020 were retrospectively analyzed.

Results: Among 910 HSCT patients, 111 patients were diagnosed as BSI within 100 days after transplantation, and 98 patients showed BSI during the period of agranulocytosis.

The complete mitochondrial genome of the edible mushroom .

The culinary-medicinal mushroom is widely cultivated in East Asia. In this study, the complete mitochondrial genome of was determined using Illumina sequencing. The circular molecule was 197,486 bp in length with a content of 25.

Large deletion of Wdr19 in developing renal tubules disrupts primary ciliogenesis, leading to polycystic kidney disease in mice.

WD repeat domain 19 (Wdr19) is a major component of the intraflagellar transport (IFT) machinery, which is involved in the function of primary cilia. However, the effects of Wdr19 on primary cilia formation, cystogenesis, and polycystic kidney disease (PKD) progression remain unclear. To study these effects, we generated three lines of kidney-specific conditional knockout mice: Wdr19-knockout (Wdr19-KO, Wdr19 ::Cdh16-Cre ), Pkd1-knockout (Pkd1-KO, Pkd1 ::Cdh16-Cre ), and Wdr19/Pkd1-double knockout (Wdr19&Pkd1-dKO, Wdr19 ;Pkd1 ::Cdh16-Cre ) mice.