A novel three-dimensional evaluation protocol to assess the consistency of critical screw angles between preoperative planning and 3d-printed patient-specific acetabular revision prostheses.

Background: Screw fixation is pivotal for prosthetic stability. For 3D-printed customized acetabular revision prostheses designed for complex, large-scale bone defects, precise adherence to preoperative screw trajectory planning is critical. However, there remains a lack of standardized three-dimensional (3D) evaluation protocols to quantify intraoperative screw angular alignment fidelity relative to preoperative digital plans, hindering universal validation criteria.

Optimization of the scale-up production process for high-yield laccase from white-rot fungi.

Introduction: Laccase exhibits significant applications in food additives, wastewater treatment, and biomass processing. Fungal laccase exhibits high activity, good stability, and excellent performance. However, scale-up production of high-yield laccase from fungi is challenging.

Latent profile analysis and influencing factors of cognitive flexibility among Chinese high school students.

With the advancement of research in educational psychology, cognitive flexibility (CF), as a pivotal ability that enables individuals to adapt to complex environments and tackle challenges, has garnered increasing attention from scholars. For high school students, there is a lack of research regarding latent profiles of cognitive flexibility and associated risk factors. To identify latent profiles of cognitive flexibility among high school students and develop targeted interventions.

Temporal Dynamics of Fecal Microbiome and Short-Chain Fatty Acids in Sows from Early Pregnancy to Weaning.

Although age-related changes in the gut microbiome of pigs have been extensively studied, the dynamic patterns of fecal microbiota and SCFAs during the gestation-to-weaning period in sows remain poorly characterized. We aim to characterize the changes in fecal microbiota and SCFAs from pregnancy to weaning, and to investigate their associations with maternal weight gain during gestation. We systematically collected 100 fecal samples at four time points (day 30 of pregnancy (T1), 1-2 days before delivery (T2), day 10 after delivery (T3), and day 21 of weaning stage (T3)), and measured the body weight of sows at T1 (132 kg ± 10.

Research Advance in the Development of Antimicrobial Peptides Using Deep Learning.

As the global issue of antibiotic resistance becomes increasingly severe, antimicrobial peptides (AMPs), a class of short-chain peptides with broad-spectrum antibacterial activity, have garnered significant attention from the scientific community due to their unique antibacterial properties and potential clinical applications. However, traditional methods for discovering and designing AMPs often rely on repetitive laboratory trials and error corrections, which are not only costly but also inefficient. In contrast, the application of artificial intelligence (AI) technology, particularly deep learning algorithms, for screening and predicting AMPs has demonstrated substantial advantages.

Off to a hard start: How job rotation reshapes newcomers' learning and adjustment process.

Although job rotation is a popular socialization practice for training newcomers, our understanding of its influence on newcomers' experience and outcomes remains limited. Extending the organizational socialization literature, we conceptualize job rotation as a planned, episodic event, which structures newcomers' learning and adjustment processes into three distinct, sequential phases: (i.e.

Multi-dimensional sleep status and trend of Chinese students aged 9-22: a large population-based study.

Sleep is an important part of life and deserves a lot of attention, especially during the teenage years. It is of great significance to analyze the current situation of adolescent sleep and its development trend in order to understand the current students' sleep situation. The participants were 44 609 students aged 9-22 years from the Chinese National Survey on Students' Constitution and Health (CNSSCH).

The Role of Circular RNA in the Progression of Gliomas and Its Potential Clinical Applications.

Circular RNAs (circRNAs) are single-stranded noncoding RNAs with a covalently closed loop structure. They are known for their stability, abundance, and highly conserved nature. Their expression is often specific to tissues or developmental stages.

MYC-Mediated Osseous Regeneration via BMSCs/PRP/β-TCP/PCL Bioprinted Constructs: Rapid Defect Rehabilitation and Preliminary Clinical Efficacy Evaluation.

Bone defects present significant clinical challenges due to their morphological heterogeneity and structural complexity, necessitating regenerative strategies that integrate structural adaptability, biomechanical stability, and osteogenic potential. In this study, a bioengineered construct composed of bone marrow mesenchymal stem cells (BMSCs), platelet-rich plasma (PRP), polycaprolactone (PCL), and β-tricalcium phosphate (β-TCP) was fabricated using 3D bioprinting. In vitro assays assessed osteoprogenitor cell proliferation (CCK-8), migration (Transwell), differentiation (ALP staining), and endothelial tubulogenesis (Matrigel assay).

Adjuvant Hepatic Arterial Infusion Chemotherapy Versus Transarterial Chemoembolization for Preventing Early Recurrence After Surgical Resection in Hepatocellular Carcinoma.

Purpose: HCC exhibits a high postoperative recurrence rate, with early recurrence (≤2 years) accounting for 70% of cases, predominantly associated with high-risk recurrence factors. Common adjuvant therapies for HCC include postoperative adjuvant hepatic arterial infusion chemotherapy (PA-HAIC) and postoperative adjuvant transarterial chemoembolization (PA-TACE). This study evaluates the comparative efficacy and safety of PA-HAIC versus PA-TACE in preventing early recurrence among HCC patients with postoperative high-risk recurrence factors.

Distal versus conventional radial artery catheterization for hemodynamic monitoring in intensive care setting: a randomized, controlled, non-inferiority trial.

Background: Arterial catheterization is fundamental for hemodynamic monitoring in critically ill patients, yet instability and loss of arterial pressure waveform remain clinically significant issues. The distal radial artery (dRA) approach has been proposed as a potential alternative to the conventional radial artery (RA) approach. This is the first randomized clinical trial to assess the comparative efficacy and reliability of dRA versus RA catheterization by examining first-attempt success rates and the stability of arterial pressure waveforms in critically ill ICU patients.

Spi1 aggravates neuropathic pain by modulating Clec7a-mediated neuroinflammation and microglial phagocytosis.

Background: Microglia are essential in mediating responses to nerve injury, with neuroinflammation and engulfment of interneuron inhibitory synapses driving heightened neuronal excitability and pain hypersensitivity. Spi1 is the main regulator of microglial homeostasis in the central nervous system. However, its function in neuropathic pain remains unclear.

Simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol in tobacco products using GC-TCD&FID in parallel method.

Moisture, 1,2-propanediol, and glycerol are crucial quality indicators in the production and application of novel tobacco products and reconstituted tobacco leaves. However, accurately measuring these components simultaneously remains a challenge. In this study, a method applying GC-TCD&FID method for the simultaneous determination of moisture, 1,2-propanediol, glycerol and menthol was developed.

Neuroimaging and biological markers of different paretic hand outcomes after stroke.

Background: Hand dysfunction significantly affects independence after stroke, with outcomes varying across individuals. Exploring biomarkers associated with the paretic hand can improve the prognosis and guide personalized rehabilitation. However, whether biomarkers derived from resting-state fMRI (rs-fMRI) can effectively classify and predict different hand outcomes and their biological mechanisms remain unclear.

Association of aortic pulse wave velocity with cardiovascular outcomes and all-cause mortality in diabetes: A systematic review and meta-analysis.

Aims: Patients with diabetes have experienced excess cardiovascular (CV) diseases. Accurate prediction of CV risk has been one of the ultimate tasks of contemporary diabetology. Aortic stiffness (AS), a key indicator of vascular health, has been increasingly considered as a valuable biomarker for CV risk and mortality prediction.

Halogenated organic pollutants in aquatic invertebrate - Fish food webs: Global distributions and trophic magnification.

Persistent halogenated organic pollutants (HOPs) are globally concerned owing to their persistence, bioaccumulation, and potential toxicity to aquatic organisms. The trophic transfer of HOPs can affect their toxic effects on both humans and ecosystems. Previous studies mostly focused on HOPs biomagnification within specific regional or ecosystem contexts, however, the variation in trophic magnification factors (TMFs) among different HOPs across a spectrum of diverse aquatic systems globally remains unclear.

Characteristics and predictive model for diffuse large B-cell lymphoma with early chemoimmunotherapy failure.

Introduction: The outcomes of refractory or relapsed diffuse large B-cell lymphoma are generally poor, especially those relapsed or progressed within 12 months from diagnosis named as early chemoimmunotherapy failure (ECF), with a 2-year OS of 24.7%. Due to the dismal outcome, early recognition of ECF and developing targeted innovative treatments to improve patient prognosis are urgent.

Research progress of hydrogel therapy to improve hypoxic environment of periodontal tissues and promote periodontal regeneration.

Periodontitis is a hypoxic infectious disease. When periodontal tissues are affected by disease and the microenvironment becomes inflamed, the release of local inflammatory factors and increased tissue metabolism lead to an imbalance between oxygen supply and demand. This inflammatory environment disrupts blood microcirculation, causing the local periodontal tissue to enter a relatively ischemic and hypoxic state.

Bioinformatics and validation reveal spinal cord cathepsin S potential degradation of perineuronal nets in neuropathic pain.

Neuropathic pain (NP), resulting from nerve damage or diseases, significantly impacts patients' quality of life. Bioinformatics analysis can assist researchers in identifying key molecular pathways involved disease progression. This study aims to investigate the hub genes in the spinal cord of NP rats, with a particular focus on the relationship between Cathepsin S (CTSS) and perineuronal nets (PNNs).

RIG-I-Inducing Small Molecules Potently Inhibit HMA-Resistant AML Through Igniting the Overloaded dsRNA Arsenal.

DNA hypomethylating agents (HMAs) are widely used to treat acute myeloid leukemia (AML)/myelodysplastic syndrome (MDS), but most treated patients relapse and lack standard treatment options. Using high-throughput screening, the approved all-trans retinoic acid (ATRA) is identified that exhibit high selectivity in killing HMA-resistant AML cells compared to parental cells. Mechanistically, HMA-resistant cells are overloaded with DNA hypomethylation-associated endogenous viral double-stranded RNA (dsRNA) which, however, fails to trigger an anticancer interferon (IFN) immune response due to downregulation of dsRNA sensor retinoic acid-inducible gene I (RIG-I).

Synthesis and bioactivity of tetrahydrothiopyran derivatives as potential acaricides against Psoroptes cuniculi.

Thirty-two tetrahydrothiopyran derivatives were synthesized, and their acaricidal activities against Psoroptes cuniculi were evaluated in vitro. The results showed that eight compounds exhibited higher acaricidal activity than ivermectin when evaluated by mass concentration, while six compounds showed superior activity when assessed by molar concentration. Compound b10 showed the lowest LC value [62.

ATF3 mediates high-salt diet-induced exacerbation of ischemic stroke: therapeutic potential of theta-burst stimulation.

Acute ischemic stroke (AIS), a high-risk condition associated with significant morbidity and disability, could be further exacerbated by a high-salt diet (HSD). Although intermittent theta-burst stimulation (iTBS) is a clinically effective rehabilitation modality, its neuroprotective mechanisms remain unclear. This study aimed to elucidate the pathways through which HSD exacerbates early AIS injury, identify potential biomarkers, and assess the impact of iTBS intervention.

High-Yield Biosynthesis Process for Producing Insulin Lispro.

Insulin lispro, a fast-acting insulin analogue, is widely used for the treatment of diabetes due to its rapid onset and high flexibility. This study established a three-step chromatography purification process, L fermentation broth can obtain 475 mg/L insulin lispro. Through a multi-step process involving fermentation, recovery of inclusion bodies, renaturation, enzymatic digestion, and chromatographic purification, a high-purity insulin lispro product was obtained.

Nonlinear association between estimated plasma volume status and acute kidney injury in acute pancreatitis patients.

Background: Acute pancreatitis (AP), a severe pancreatic inflammatory condition, with a mortality rate reaching up to 40%. Recently, AP shows a steadily elevating prevalence, which causes the greater number of hospital admissions, imposing the substantial economic burden. Acute kidney injury (AKI) complicates take up approximately 15% of AP cases, with an associated mortality rate of 74.