Bone marrow immune remodeling in depression: TNF/NF-κB mediated leukocyte redistribution and construction of an interpretable predictive model.

Environmental stress contributes to the development of depression through neuro-immune interactions, yet the underlying molecular mechanisms and associated clinical diagnostic biomarkers remain unclear. We established a psychosocial stress mouse model and systematically investigated the immune dysregulation induced by stress through integrated analysis of blood cell profiles, leukocyte transcriptomics, protein-protein interaction networks, single-cell RNA sequencing, and targeted pharmacological intervention. Additionally, we constructed and validated a depression predictive model using multiparametric peripheral blood data and machine learning, and assessed feature importance using the SHapley Additive exPlanations (SHAP) analysis.

Butyric acid and valeric acid attenuate stress-induced ferroptosis and depressive-like behaviors by suppressing hippocampal neuroinflammation.

Background: Depression is closely associated with stress-induced hippocampal damage and dysfunction. Emerging evidence demonstrates that the gut microbiota and its metabolites, acting as probiotics or prebiotics, can modulate brain structure and function via the gut-brain axis, thereby offering therapeutic potential for ameliorating related neurological and psychiatric disorders. This study delves into the contribution of the gut microbiota and its metabolites to stress-induced ferroptosis of hippocampal neurons and the associated molecular pathways.

Identification of a novel gene cluster responsible for 2-isopropylphenol degradation in strain sp. D-6.

Isoprocarb (IPC), a widely used carbamate insecticide, causes environmental contamination and poses risks to humans and ecosystems. sp. D-6, capable of utilizing IPC as the sole growth substrate, was isolated by our lab.

Qing'e Pills Ameliorates Osteoporosis by Regulating Gut Microbiota and Th17/Treg Balance in Ovariectomized Rats.

Purpose: Bone metabolism disorders are strongly associated with T helper type 17/regulatory T (Th17/Treg) cell imbalance and inflammatory dysregulation. Qing'e pills (QEP) is a classical prescription for treating osteoporosis with both safety and clinical effectiveness. However, the mechanism of its immune action remains unclear.

sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.

A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.

Stress-Induced Cholesterol Metabolic Dysregulation and Differentiation Trajectory Shift in Oligodendrocytes Synergistically Drive Demyelination.

Stress-induced demyelination resulting from oligodendrocyte (OLG) dysfunction is one of the key pathological mechanisms of depression, yet its dynamic regulatory network remains unclear. This study integrates single-cell transcriptomics, lineage tracing, and functional interventions to uncover a temporally disordered OLG cholesterol metabolism in a restraint stress mouse model: After 3 days of stress, upregulation of efflux genes Abca1/Abcg1 triggers a compensatory response; however, by day 14, persistent suppression of transport genes (, ) and homeostatic regulatory genes (, , etc.) leads to intracellular accumulation of "ineffective cholesterol", with compensatory activation of the AMPK pathway unable to restore cholesterol conversion into myelin.

Nanostructured conductive polymers: synthesis and application in biomedicine.

Conductive polymers (CPs), distinguished by their sp-hybridized carbon backbone, offer remarkable electrical conductivity while maintaining the advantageous mechanical flexibility and processing characteristics typical of organic polymers. Compared to their bulk counterparts, nanostructured CPs exhibit unique physicochemical properties, such as large surface areas and shortened charge/mass transport pathways, making them promising candidates for various applications. This mini review explores various synthesis methodologies for nanostructured CPs, including electrospinning, hard templating, and soft templating techniques, while elucidating their advantages and disadvantages.

The Gut Microbiota Metabolite Butyrate Modulates Acute Stress-Induced Ferroptosis in the Prefrontal Cortex via the Gut-Brain Axis.

Stress has been implicated in the onset of mental disorders such as depression, with the prefrontal cortex (PFC) playing a crucial role. However, the underlying mechanisms remain to be fully elucidated. Metabolites secreted by intestinal flora can enter the bloodstream and exert regulatory effects on the body.

Unveiling the degradation mechanism of 3,5-dichloroaniline: Activated sludge acclimation, strain isolation and gene cloning.

3,5-Dichloroaniline (3,5-DCA) is extensively used in synthesizing dicarboximide fungicides, medical compounds and dyes. Due to its widespread use in agriculture and industry, 3,5-DCA is often detected in groundwater, wastewater, sediments and soil, posing great risk to animals and humans. However, the genes and enzymes involved in 3,5-DCA degradation remain unidentified.

SAT1/ALOX15 Signaling Pathway Is Involved in Ferroptosis After Skeletal Muscle Contusion.

Skeletal muscle contusion (SMC) is common in daily life and clinical practice, but the molecular mechanisms underlying SMC healing are unclear. Ferroptosis, a regulated cell death type, has gained attention recently. We observed iron overload in skeletal muscle following contusion through HE and Perls staining.

Remediation of isoproturon-contaminated soil by Sphingobium sp. strain YBL2: Bioaugmentation, detoxification and community structure.

The widely used phenylurea herbicide isoproturon (IPU) and its residues can inhibit the growth of subsequently planted crops. However, reports on bioremediation of IPU-contaminated soil are scarce. In this study, Sphingobium sp.

VISTA antibody-loaded FeO@TiO nanoparticles for sonodynamic therapy-synergistic immune checkpoint therapy of pancreatic cancer.

Breaking the poor permeability of immune checkpoint inhibitors (ICIs) caused by the stromal barrier and reversing the immunosuppressive microenvironment are significant challenges in pancreatic cancer immunotherapy. In this study, we synthesized core-shell FeO@TiO nanoparticles to act as carriers for loading VISTA monoclonal antibodies to form FeO@TiO@VISTAmAb (FTV). The nanoparticles are designed to target the overexpressed ICIs VISTA in pancreatic cancer, aiming to improve magnetic resonance imaging-guided sonodynamic therapy (SDT)-facilitated immunotherapy.

Highly Anti-Markovnikov Selective Oxidative Arene Alkenylation Using Ir(I) Catalyst Precursors and Cu(II) Carboxylates.

The Ir(I) complex [Ir(μ-Cl)(coe)] (coe = cyclooctene) is a catalyst precursor for benzene alkenylation using Cu(II) carboxylate salts. Using [Ir(μ-Cl)(coe)], propenylbenzenes are formed from the reaction of benzene, propylene, and CuX (X = acetate, pivalate, or 2-ethylhexanoate). The Ir-catalyzed reactions selectively produce anti-Markovnikov products, -β-methylstyrene, -β-methylstyrene, and allylbenzene, along with minor amounts of the Markovnikov product, α-methylstyrene.

Numerical simulation of strength and failure analysis of heterogeneous sandstone under different loading rates.

Natural rock masses often contain heterogeneous structures with varying sizes, non-uniform distributions, and strengths, which influence the mechanical response characteristics and crack propagation modes under loading. Furthermore, heterogeneous structures can affect the stability of the rock mass, in serious cases, leading to geotechnical and mining engineering disasters. In the present work, a parallel-bond model (PBM)-based numerical simulation using Particle Flow Code (PFC) was carried out to study the strength and failure characteristics of sandstone specimens with heterogeneous structures under different loading rates.

Pd(II) and Rh(I) Catalytic Precursors for Arene Alkenylation: Comparative Evaluation of Reactivity and Mechanism Based on Experimental and Computational Studies.

We combine experimental and computational investigations to compare and understand catalytic arene alkenylation using the Pd(II) and Rh(I) precursors Pd(OAc) and [(η-CH)Rh(μ-OAc)] with arene, olefin, and Cu(II) carboxylate at elevated temperatures (>120 °C). Under specific conditions, previous computational and experimental efforts have identified heterotrimetallic cyclic PdCu(η-CH)(μ-OPiv) and [(η-CH)Rh(μ-OPiv)](μ-Cu) (OPiv = pivalate) species as likely active catalysts for these processes. Further studies of catalyst speciation suggest a complicated equilibrium between Cu(II)-containing complexes containing one Rh or Pd atom with complexes containing two Rh or Pd atoms.

Ginsenoside Rk1-Loaded Manganese-Doped Hollow Titania for Enhancing Tumor Sonodynamic Therapy Upregulation of Intracellular Reactive Oxygen Species.

Amplifying the intracellular reactive oxygen species (ROS) level remains an urgent challenge for efficient sonodynamic therapy (SDT) of tumors. Herein, by loading ginsenoside Rk1 with manganese-doped hollow titania (MHT), a Rk1@MHT sonosensitizer was conceived to strengthen the outcome of tumor SDT. The results verify that manganese-doping remarkably elevates the UV-visible absorption and decreases the bandgap energy of titania from 3.

Load bearing capacity of arch structure in unconsolidated layers.

Coal mining inevitably results in the movement of overlying strata, with the upward formation of the strata leading to surface subsidence, causing irreversible impact on the buildings, land, and ecological environment. The movement and deformation of the strata are controlled by the bearing structure in the overlying strata, whose failure results in the deformation and breakage of the overlying strata simultaneously. While studies have been conducted on the arch structure in unconsolidated layers (ASUL), its bearing performance has not been addressed.

[Research strategies and methods for precious animal medicine substitutes].

Animal medicine is a large category of Chinese medicinecommonly used in clinical practice and has important scientific and therapeutic value. Animal medicine isscarcer than herbal medicine. In recent years, with the vigorous development of traditional Chinese medicine(TCM),the contradiction between the increasing industrial demand andsupply of scarce and even endangered medicinal animals has become increasingly prominent.