Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis.

The accumulation of amyloid fibrils has been identified in tissues outside the brain, yet little is understood about the formation of extracerebral amyloidosis and its impact on organ aging. Here, we demonstrate that both transgenic Alzheimer's disease (AD) mice and naturally aging mice exhibit accumulated senescent bone marrow adipocytes (BMAds), accompanied by amyloid deposits. Senescent BMAds acquire a secretory phenotype, markedly increasing secretion of serum amyloid P component (SAP), also known as pentraxin 2 (PTX2).

Comparing Kangaroo Mother Care and Expressed Breast Milk as Non-pharmacological Strategies for Reducing Procedural Pain in Neonates: A Randomized Controlled Trial.

Background: Neonates in neonatal intensive care units frequently endure painful procedures, such as adhesive tape removal, often without adequate pain management. Non-pharmacological interventions like kangaroo mother care (KMC) and expressed breast milk (EBM) administration have shown promise in alleviating neonatal pain. However, comparative data on their efficacy for specific procedures are limited.

Identification of KMH-45, a novel MRGPRX2 inhibitor with enhanced anti-pruritic properties.

Pruritus, or itch, is a common complaint in dermatology, adversely affecting patient's well-being and becoming unbearable when severe. Histamine induces itch by binding to the histamine receptor and antihistamines alleviate itch symptoms by blocking this action in many cases. However, the insufficient relief provided by antihistamines in various chronic conditions necessitates the development of anti-pruritic agents beyond the histamine mechanisms.

Investigations Into the Outbreak of Chandipura Virus Encephalitis, Gujarat, India, 2024.

Chandipura virus, a Vesiculovirus, was first identified in India during an outbreak investigation of acute febrile illness in 1965. The virus first gained global attention after being implicated as the causative agent of an outbreak of acute viral encephalitis characterized by rapid clinical deterioration and death, in 2003. Since then, the virus has caused multiple outbreaks in central and western Indian states.

Odor classification: Exploring feature performance and imbalanced data learning techniques.

This research delves into olfaction, a sensory modality that remains complex and inadequately understood. We aim to fill in two gaps in recent studies that attempted to use machine learning and deep learning approaches to predict human smell perception. The first one is that molecules are usually represented with molecular fingerprints, mass spectra, and vibrational spectra; however, the influence of the selected representation method on predictive performance is inadequately documented in direct comparative studies.

Laparoscopic Vesicovaginal Fistula Repair Using Kumar's Knotless Technique: Short-Term Experience From a Tertiary Care Center.

Introduction: The laparoscopic (lap) technique is well-established for the repair of vesicovaginal fistula (VVF). However, its main limitation is a lack of expertise in intracorporeal suturing. This retrospective study presents a lap VVF repair using Kumar's knotless technique with V-Loc™ (Medtronic, MN, USA) barbed suture and compares its results with the open VVF repair technique.

Quality of Life and Nutritional Outcomes with PEG Feeding in Locally Advanced Oral Cancer: An Institutional Experience.

Unlabelled: Locally advanced oral carcinoma requires extensive surgical intervention, leading to significant postoperative nutritional challenges. Percutaneous Endoscopic Gastrostomy (PEG) feeding has emerged as a preferred method over nasogastric (NG) feeding, providing consistent nutritional support that aids recovery by reducing complications and enhancing quality of life. This prospective cohort study followed 50 patients with locally advanced oral carcinoma requiring postoperative PEG feeding.

Correction: Computational reassessment of RNA-seq data reveals key genes in active tuberculosis.

[This corrects the article DOI: 10.1371/journal.pone.

Structure-Based QSAR Modeling of RET Kinase Inhibitors from 49 Different 5,6-Fused Bicyclic Heteroaromatic Cores to Patent-Driven Validation.

RET receptor tyrosine kinase is crucial for nerve and tissue development but can be an important oncogenic driver. This study focuses on exploring the design principles of potent RET inhibitors through molecular docking and 3D-QSAR modeling of 5,6-fused bicyclic heteroaromatic derivatives. First of all, RET inhibitors of 49 different bicyclic substructures were collected from five different data sources and selected through molecular docking simulations.

Use of Synthetic Membranes and Bovine Blood in Artificial Feeding Systems of Mosquitoes Improves Feeding Rates: Results from a Systematic Review and Meta-Analysis.

Artificial blood feeding of mosquitoes is essential for their rearing in insectaries as well as for mosquito-borne pathogen transmission experiments. We conducted a systematic review and meta-analysis of the different artificial feeding systems available for mosquitoes to synthesize evidence regarding their efficacy in terms of feeding rates and fecundity. PubMed, Scopus, Web of Science, and Google Scholar were systematically searched to retrieve 1,822 experimental studies assessing the efficacy of artificial feeding systems.

Unlocking the potential of miRNAs in detecting pulmonary tuberculosis: prospects and pitfalls.

Tuberculosis (TB) is one of the deadliest infectious diseases globally, ranking as 13th leading cause of mortality and morbidity. According to the Global Tuberculosis Report 2022, TB claimed the lives of 1.6 million people worldwide in 2021.

NO classifier prediction of anti neuroinflammatory agents using text mining of 3D molecular fingerprints.

CNS Drug discovery has been challenging due to the lack of clarity on CNS diseases' basic biological and pathological mechanisms. Despite the difficulty, some CNS drugs have been developed based on phenotypic effects. Herein, we propose a phenotype-structure relationship model, which predicts an anti-neuroinflammatory potency based on 3D molecular structures of the phenotype-active or inactive compounds without specifying targets.

Side Chain Investigation of Imidazopyridazine as a Hinge Binder for Targeting Actionable Mutations of RET Kinase.

Actionable mutations of RET kinase have been identified as oncogenic drivers of solid tumors, including thyroid cancer, metastatic colorectal cancer, and nonsmall cell lung cancer. Although multikinase inhibitors and RET selective inhibitors are used to treat patients with RET alterations, there is insufficient research addressing certain issues: which actionable mutations arise from these therapies, how to improve the clinical response rate to RET inhibitors, and how to design new inhibitors to overcome drug resistance. Therefore, the development of sophisticated tool compounds is required to investigate the molecular mechanisms of actionable mutations and to develop breakthrough therapeutics for different RET alterations.

A qualitative analysis to identify the issues of tuberculosis management in tribal areas of Rajasthan.

Background: Tuberculosis (TB) is a disease with social issue. Tribal people are disproportionately affected by TB. There is a scarcity of data on issues of TB management among the tribal groups of India.

Serum Amyloid P Secreted by Bone Marrow Adipocytes Drives Skeletal Amyloidosis.

The accumulation of amyloid fibrils has been identified in tissues outside the brain, yet little is understood about the formation of extracerebral amyloidosis and its impact on the aging process of these organs. Here, we demonstrate that both transgenic mice modeling Alzheimer's disease (AD) and naturally aging mice exhibit accumulated senescent bone marrow adipocytes (BMAds), accompanied by amyloid deposits surrounding the BMAds. Senescent BMAds acquire a secretory phenotype, resulting in a marked increase in the secretion of serum amyloid P component (SAP), also known as pentraxin 2 (PTX2).

Transcriptomic response of lumpfish () head kidney to viral mimic, with a focus on the family.

The economic importance of lumpfish () is increasing, but several aspects of its immune responses are not well understood. To discover genes and mechanisms involved in the lumpfish antiviral response, fish were intraperitoneally injected with either the viral mimic polyinosinic:polycytidylic acid [poly(I:C)] or phosphate-buffered saline (PBS; vehicle control), and head kidneys were sampled 24 hours post-injection (hpi) for transcriptomic analyses. RNA sequencing (RNA-Seq) (adjusted p-value <0.

Evaluation of the Tensor Fascia Lata Muscle Pedicle Bone Grafting Technique in the Management of Avascular Necrosis of the Femoral Head: An Observational Clinical Study.

Background: Osteonecrosis of the hip is defined as necrosis of the bone tissue due to some form of vascular insult, subsequently leading to the collapse of the femoral head and secondary osteoarthritis, which leads to pain and impaired joint function. This disease is widely known to affect middle-aged groups; however, in the Indian population, even younger people are more commonly affected. The disease has a debilitating effect on the activities of daily living (ADL) and the productivity of individuals and has financial consequences.

Combatting Antibiotic Resistance: Identifying Gaps in Knowledge, Attitude, and Practice Among Medical Interns.

Introduction: Antibiotic resistance presents a significant global health threat to modern medicine. The awareness and attitude of future doctors undergoing training may play a crucial role in addressing this important issue, influencing the control of resistance and promoting responsible antibiotic stewardship. This study aimed to estimate knowledge, attitudes, and practices regarding antibiotic usage and antimicrobial resistance among tertiary care teaching hospital medical interns.

Determination of Species Identity and Genetic Diversity of Aedes aegypti and Other Medically Important Mosquitoes of India Using DNA Barcoding.

Aedes aegypti-borne viruses (i.e., dengue, chikungunya, and Zika) have become endemic to India, posing a severe threat to public health.

Computational reassessment of RNA-seq data reveals key genes in active tuberculosis.

Background: Tuberculosis is a serious life-threatening disease among the top global health challenges and rapid and effective diagnostic biomarkers are vital for early diagnosis especially given the increasing prevalence of multidrug resistance.

Methods: Two human whole blood microarray datasets, GSE42826 and GSE42830 were retrieved from publicly available gene expression omnibus (GEO) database. Deregulated genes (DEGs) were identified using GEO2R online tool and Gene Ontology (GO), protein-protein interaction (PPI) network analysis was performed using Metascape and STRING databases.

Exploring Marine Alkaloids: A Natural Approach to Cancer Treatment.

Cancer is one of the most complicated and prevalent diseases in the world, and its incidence is growing worldwide. Natural products containing pharmacological activity are widely used in the pharmaceutical industry, especially in anticancer drugs, due to their diverse structures and distinctive functional groups that inspire new drug results by means of synthetic chemistry. Terrestrial medicinal plants have traditionally been the primary source for developing natural products (NPs).