Antimigration and Anti-Invasion Properties of Extract, an Endophyte Isolated From L. on Non-Small-Cell Lung Cancer Cells: Experiments and Methods.

Endophytic fungi are microorganisms that infect living plant tissues internally without producing obvious symptoms of infection, existing in a symbiotic relationship with plants for a portion of their life cycle. Currently, endophytic fungi serve as alternate sources for the production of new bioactive chemicals with great efficacy. This study aimed to examine the antimigration and anti-invasion capabilities of the endophytic fungus extract, isolated from L.

Evaluation of the antibacterial efficacy of combinations of Garcinia mangostana, Curcuma comosa, and Acanthus ebracteatus for acne vulgaris treatment: in Silico and in vitro validation.

Acne vulgaris (AV) is a common, long-lasting skin condition characterized by inflammation that is frequently linked to the bacteria Cutibacterium acnes (C. acnes) and Staphylococcus spp. Conventional medicines face difficulties, such as bacterial resistance, in treating acne vulgaris, highlighting the need for new treatment approaches.

Antibacterial activity of the endophytic fungal extracts and synergistic effects of combinations of ethylenediaminetetraacetic acid (EDTA) against and .

The growing threat of antibiotic resistance in bacteria is a critical public health concern. Combining natural compounds with antimicrobial agents is an alternative approach to improve the antibacterial efficacy and safety of these agents. The strategy is to restore the effectiveness of existing antibiotics while minimizing the required concentrations of antibiotics or antimicrobial agents.

Multi-locus sequence typing and genetic diversity of antibiotic-resistant genes and virulence-associated genes in Burkholderia pseudomallei: Insights from whole genome sequencing of animal and environmental isolates in Thailand.

Burkholderia pseudomallei is a Gram-negative bacillus and the etiological agent of melioidosis in humans and animals. The disease is highly endemic in northern Australia and Southeast Asia. Comprehensive genomic data are essential for understanding the bacteria's dissemination and genetic relationships among strains from different geographical regions.

Diagnostic accuracy of automation and non-automation techniques for identifying Burkholderia pseudomallei: A systematic review and meta-analysis.

Background: Burkholderia pseudomallei, a Gram-negative pathogen, causes melioidosis. Although various clinical laboratory identification methods exist, culture-based techniques lack comprehensive evaluation. Thus, this systematic review and meta-analysis aimed to assess the diagnostic accuracy of culture-based automation and non-automation methods.

A systematic review and meta-analysis of the global prevalence and relationships among sequence types isolated from humans, animals, and the environment.

Background And Aim: , a highly pathogenic bacterium responsible for melioidosis, exhibits ecological ubiquity and thrives within soil and water reservoirs, posing significant infection risks to humans and animals through direct contact. The aim of this study was to elucidate the genetic diversity and prevalence patterns of sequence types (STs) across a global spectrum and to understand the relationships between strains isolated from different sources.

Materials And Methods: We performed a systematic review and meta-analysis in this study.

High prevalence and genetic diversity of a single ancestral origin azole-resistant Aspergillus fumigatus in indoor environments at Walailak University, Southern Thailand.

This study explored the prevalence, genetic diversity, and population structure of azole-resistant Aspergillus fumigatus (ARAf) at Walailak University in Southern Thailand. Three hundred samples were collected from dwellings and workplaces, screened for azole resistance, and tested for drug susceptibility. Molecular detection of alterations in the cyp51A gene and CSP1 typing was performed.

Evaluation of Susceptibility of the Human Pathogen to the Antibiotic Capreomycin.

infection causes gastritis, peptic ulcer disease, mucosa-associated lymphoid tissue lymphoma, and gastric cancer and can also promote thrombosis. It is estimated that approximately 4.5 billion individuals are infected, thus rendering the most prevalent microbial pathogen.

Extract Has Antiamoebic Activity to Trophozoites and Changes Expression of Autophagy-Related Genes.

, a well-known medicinal plant, has been used for several therapeutic purposes as it contains numerous pharmacological active compounds. Our study reported an anti-parasitic activity of seed extract against . The stress induced by the extract on the surviving trophozoites for encystation and vacuolization was examined by microscopy, and transcriptional expression of autophagy-related genes was investigated by quantitative PCR.

Use of Recombinant Strains in Immunofluorescence Assays for Melioidosis Diagnosis.

is a Gram-negative bacterium and the causative agent of melioidosis in humans and animals in the tropics. The clinical manifestations of melioidosis are diverse, ranging from localized infections to whole-body sepsis. The effective serological method is crucial for the point-of-care diagnosis of melioidosis.

Amoebicidal activity of extract and its effect on autophagy-related gene expression at the transcriptional level.

Cassia angustifolia Vahl. plant is used for many therapeutic purposes, for example, in people with constipation, skin diseases, including helminthic and parasitic infections. In our study, we demonstrated an amoebicidal activity of C.

Cross-reactivity of latex agglutination assay complicates the identification of Burkholderia pseudomallei from soil.

The monoclonal antibody-based latex agglutination tests targeting a high molecular weight exopolysaccharide antigen of Burkholderia pseudomallei are commercially available. The tests are primarily used in routine diagnosis of melioidosis in major hospitals in Thailand and some endemic countries. Being a rapid test, this technique was employed as a presumptive test to identify colonies of B.

A new biphasic test for the detection of Helicobacter pylori in gastric biopsies.

The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H.

Enhancement of viable Campylobacter detection by chemotactic stimuli.

The effects of chemotactic stimuli on motility ability of viable Campylobacter to pass through a 0.45 microm pore size filter in viscous condition were investigated. Reference strains including C.

A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products.

Conventional procedures for isolation of thermophilic Campylobacter spp. from chicken are complex, labor intensive, and time-consuming. The objective of this study was to create a novel Campylobacter culturing apparatus.