Publications by authors named "Chatruthai Meethai"
Food contamination with non-typhoidal (NTS) presents a significant public health risk, underscoring the critical need for rigorous food safety measures throughout the production, distribution, preparation, and consumption stages. Conventional diagnostic strategies are time-consuming and labor-intensive and are thus sub-optimal for throughput NTS detection. Bacteriophages (phages) are highly specialized bacterial viruses and exhibit extreme specificity for their hosts.
View Article and Find Full Text PDFArticle Synopsis
- Burkholderia pseudomallei is a bacterium that causes melioidosis, and the stress hormone epinephrine increases its movement and flagellar gene activity.
- Researchers created a mutant strain lacking the QseBC system, which resulted in decreased bacterial motility, biofilm formation, and survival inside immune cells.
- Although epinephrine still boosted motility in the mutant, QseBC is not crucial for this response, indicating its specific regulatory role in bacterial behavior.
Article Synopsis
- The Burkholderia pseudomallei cluster includes several species, primarily pathogenic ones like B. pseudomallei and B. mallei, which cause melioidosis and glanders, respectively.
- Researchers developed a multiplex PCR assay to detect these pathogenic species and their variants, successfully distinguishing them from non-pathogenic strains.
- Field tests demonstrated that the PCR method is highly sensitive and specific, making it an effective tool for studying the epidemiology of these bacteria in soil environments.
Phospholipase C (PLC) enzymes are key virulence factors in several pathogenic bacteria. Burkholderia pseudomallei, the causative agent of melioidosis, possesses at least three plc genes (plc1, plc2 and plc3). We found that in culture medium plc1 gene expression increased with increasing pH, whilst expression of the plc3 gene was pH (4.
View Article and Find Full Text PDFA new biphasic test for the detection of Helicobacter pylori in gastric biopsies.
J Microbiol Methods
January 2014
The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H.
View Article and Find Full Text PDF