Serum bone turnover biomarkers in early postoperative period related to the spontaneous resolution of disc herniation.

A new way of induction surgery to obtain induced resorption of herniated nucleus pulposus (iRHNP) was recently proposed by our team, both cervical or lumbar disc herniation (CDH or LDH), when bony repair of the decompression groove simultaneously started, suggesting the possible relation to iRHNP mechanism. Since some biomarkers from peripheral blood are regarded as indicators of bone turnover, the role of circulating biomarkers for iRHNP that may reveal the mechanism remains to be determined. This study aimed to identify whether potential biomarkers changed in coincidence with the degree of iRHNP in CDH and LDH after induction surgery.

Design and validation of nurses' knowledge, attitude and practice questionnaire for discharge planning of stroke patients.

Objective: The aim of this study was to design a national and culturally appropriate questionnaire to assess the nurses' knowledge, attitude and practice (KAP) for discharge planning of stroke patients and to test the reliability and validity of this questionnaire.

Methods: This questionnaire was developed using domestic and international literature and combining the KAP theory. It covered eight aspects of basic knowledge, relevant knowledge, psychological cognition, emotional tendency, intentional behavior, assessment, implementation and evaluation of discharge planning for stroke patients.

The Integrated Function of the Lateral Hypothalamus in Energy Homeostasis.

The lateral hypothalamic area (LHA) serves as a central integrative hub for the regulation of energy homeostasis and motivational behaviors, including feeding and arousal. Recent advances in single-cell transcriptomics have revealed remarkable molecular heterogeneity within the LHA, identifying more than 30 distinct neuronal subtypes, such as GABAergic (LHA), glutamatergic (LHA), orexin, melanin-concentrating hormone (MCH), and leptin receptor-expressing (LHA) neurons. These neuronal populations sense peripheral metabolic signals-such as leptin, insulin, and glucose-both directly and indirectly, and they coordinate appropriate physiological and behavioral responses through local circuits and reciprocal connections with other hypothalamic nuclei.

A new surgery choice of bilateral laminoplasty for symptomatic three or more-level lumbar canal stenosis in patients over 60 years old: a two-year retrospective study.

Introduction: Multi-level lumbar canal stenosis (LCS) in patients over 60 years is common and frequently complicated by significant comorbidities, making surgical decisions complex. While posterior lumbar interbody fusion (PLIF) is often used, bilateral laminoplasty offers a potential motion-preserving alternative. This study compares outcomes of bilateral laminoplasty versus PLIF for multi-level LCS in this elderly population.

Investigation of Sleep Disorders and Related Influencing Factors Among the Elderly in Southeast Coastal Regions of China: A Cross-Sectional Survey Analysis.

Objective: To assess the sleep status and prevalence rate of the elderly in southeast coastal areas of China, and analyze related factors.

Methods: A questionnaire survey was conducted among elderly individuals in Wenzhou City, Zhejiang Province, situated in the southeast coastal areas of China, using a multi-stage random sampling method. A total of 903 elderly people aged ≥ 60 years were selected for the survey.

Irisin reprograms microglia through activation of STAT6 and prevents cognitive dysfunction after surgery in mice.

Postoperative cognitive dysfunction (POCD) is common in the aged population and associated with poor clinical outcomes. Irisin, an endogenous molecule that mediates the beneficial effects of exercise, has shown neuroprotective potential in several models of neurological diseases. Here we show that preoperative serum level of irisin is reduced in dementia patients over the age of 70.

Establishment and validation of predictive model of prostate cancer.

Prostate cancer is a prevalent malignant disease among middle-aged and elderly men. Its prevention and detection are significant public health issues. We aimed to construct an interpretable model for predicting death risk in prostate cancer patients.

Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers.

The adipogenic property of decellularized adipose-derived matrix (DAM) varies widely across reports, making it difficult to make a horizontal comparison between reports and posing challenges for the stable clinical translation of DAM. It is possibly due to differences in donor characteristics, but the exact relationship remains unclear. Despite extensive research on the differences between superficial and deep layers of abdominal subcutaneous fat, a main donor of DAM, little is known about their extracellular matrix (ECM) which is promising in regenerative medicine.

Regenerated fat induced by a decellularized adipose matrix can survive long-term in vivo.

Decellularized adipose matrix (DAM) is considered to be the most potential biological scaffold for soft tissue repair and reconstruction, as it is able to induce the regeneration of adipose tissue in situ in adulthood. But how does this adipose tissue regeneration happen and develop in vivo? Is it the same as the original autologous one? Temporary existence or long-term survival? These are the key questions that will determine the future applications of DAM. In this study, we investigated the composition, structure and biomechanical properties of DAM before implanting it into the subcutaneous back of immunodeficient mice.

Hydrophilic Components as Key Active Ingredients in Adipose-Derived Matrix Bioscaffolds for Inducing Fat Regeneration.

Decellularized adipose-derived matrix (DAM) has emerged as a promising biomaterial for soft tissue reconstruction. However, due to a lack of research on its complex composition, the understanding of the key components in DAM remains limited, leading to inconsistent adipogenic properties and challenges in optimizing preparation methods purposefully. In this study, it is proposed for the first time that DAM comprises two distinct components: hydrophilic (H-DAM) and lipophilic (L-DAM), each with markedly different effects on fat regeneration.

DSCR1-1 attenuates osteoarthritis-associated chondrocyte injury by regulating the CREB1/ALDH2/Wnt/β-catenin axis: An in vitro and in vivo study.

The progression of osteoarthritis (OA) includes the initial inflammation, subsequent degradation of the extracellular matrix (ECM), and chondrocyte apoptosis. Down syndrome candidate region 1 (DSCR1) is a stress-responsive gene and expresses in varied types of cells, including chondrocytes. Bioinformatics analysis of GSE103416 and GSE104739 datasets showed higher DSCR1 expression in the inflamed cartilage tissues and chondrocytes of OA.

Effects of nanographene oxide on adipose-derived stem cell cryopreservation.

Cryoinjury mitigation is key in cell cryopreservation. Here, we aimed to assess the effectiveness of nanographene oxide (nano-GO) for improving cryoprotectant agents (CPAs) in human adipose stem cell (hADSC) cryopreservation. For in vitro experiments, nano-GO (5 μg/mL) was added to the CPAs in the control, and passage (P) 2 hADSCs were collected and cryopreserved for around two weeks.

Impaired neuronal macroautophagy in the prelimbic cortex contributes to comorbid anxiety-like behaviors in rats with chronic neuropathic pain.

A large proportion of patients with chronic pain experience co-morbid anxiety. The medial prefrontal cortex (mPFC) is proposed to underlie this comorbidity, but the molecular and neuronal mechanisms are not fully understood. Here, we reported that impaired neuronal macroautophagy in the prelimbic cortical (PrL) subregion of the mPFC paralleled the occurrence of anxiety-like behaviors in rats with chronic spared nerve injury (SNI).

Study on the Photocatalytic Properties of Metal-Organic Framework-Derived C-, N-Co-Doped ZnO.

In this study, C- and N-co-doped ZnO photocatalysts were prepared through pyrolysis using metal-organic frameworks (MOFs) as precursor materials. The crystal structure, morphology, and surface chemical composition of the samples were characterised via X-ray diffraction (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). Their activities in photocatalytic reactions were also evaluated through photocatalytic experiments.

Comparison of the quality of Nurse-Led palliative care with standard medical care during six months in 405 patients with Parkinson's disease and burdens of their Caregivers: A retrospective study at a single center in China.

Background: Palliative care is mainly used to improve the quality of life of patients with chronic diseases by addressing their medical conditions and psychological problems. End-stage Parkinson's disease (PD) is also a progressive disease like cancer and could be managed by palliative care. This study was conducted at a single center in China and aimed to compare the quality of nurse-led palliative care with standard medical care during six months in 405 patients with Parkinson's disease (PPD) and their caregivers using the Chinese version of the 39-item Parkinson's Disease Questionnaire (PDQ-39) and the Chinese Zarit Burden Interview (ZBI) scale.

Harnessing fine fibers in decellularized adipose-derived matrix for enhanced adipose regeneration.

Decellularized Adipose-Derived Matrix (DAM) has the function of inducing adipogenesis, but the distribution of adipogenesis is uneven. We found for the first time that DAM contains two structural components: The tough fibers DAM (T-DAM) and the fine fibers DAM (F-DAM). T-DAM was a dense vortex structure composed of a large number of coarse fibers, characterized by myoblast-related proteins, which cannot achieve fat regeneration and forms a typical "adipose-free zone".

Transcriptomic and machine learning analyses identify hub genes of metabolism and host immune response that are associated with the progression of breast capsular contracture.

Capsular contracture is a prevalent and severe complication that affects the postoperative outcomes of patients who receive silicone breast implants. At present, prosthesis replacement is the major treatment for capsular contracture after both breast augmentation procedures and breast reconstruction following breast cancer surgery. However, the mechanism(s) underlying breast capsular contracture remains unclear.

Effectiveness of a New Enzyme-Free Method for the Preparation of a Decellularized Adipose-Derived Matrix.

Background: Decellularized adipose-derived matrix (DAM) represents a new alternative to tissue fillers. The function of DAM is closely associated with the decellularization technique used for its preparation. However, most techniques are time-consuming and expensive, and this might reduce the popularity of DAM.

Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived Matrix.

Background: The decellularized adipose-derived matrix (DAM) has emerged as a promising biomaterial for inducing adipose tissue regeneration. Various methods have been employed to produce DAM, among which the enzyme-free method is a relatively recent preparation technique. The mechanical fragmentation step plays a crucial role in determining the efficacy of the enzyme-free preparation.

Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose-derived matrix.

Background: Decellularized adipose-derived matrix (DAM), a biological scaffold that can induce adipose regeneration. The balance between its sterilization efficiency and its ability to maintain in situ adipose regeneration should be considered in terminal sterilization. The purpose of this study was to investigate the effects of radiation sterilization of cobalt-60 ( Co)with different doses on adipogenesis induced by different forms of DAM, so as to reduce radiation dose under the premise of safe and effective sterilization and ensure adipogenesis induced by DAM in vivo.

A Bibliometric Analysis of Research on Decellularized Matrix for Two Decades.

The articles and reviews in the field of decellularized extracellular matrix (dECM) from 2001 to 2021 were retrieved and extracted from the Web of Science Core Collection. The R package Bibliometrix, CiteSpace, VOSviewer, and the online BIBLIOMETRC platform were utilized for bibliometric analysis, including specific characteristics of annual publications, influential countries/regions, core journals, leading institutions, keywords, key references, cocited authors, journals and institutions, cooperation, and historical direct citations. Our study concluded core references that fueled the development of dECM and highlighted current research directions, hotpots, and trends.

Multi-functional gene ZNF281 identified as a molecular biomarker in soft tissue regeneration and pan-cancer progression.

Regeneration and tumorigenesis are indicated as related processes, while regeneration leads to life and the outcome of tumorigenesis is death. Here, we show the upregulation of (zinc finger 281) in our adipose regeneration model through RNA-seq analysis. Then, we validated the upregulation of zfp281 in adipose regeneration immunofluorescence.