Iodine uptake variability as a predictive biomarker for radioiodine therapy outcomes in Graves Disease: Validation through multi-time-point RAIU assessment in a 1249-patient cohort.

Aims: To investigate the impact of iodine uptake variability (IUV) as a predictive biomarker associated with Graves Disease (GD) on the success rate of radioiodine (RAI) therapy for GD within 36 months, and discover IUV indicators for predicting GD prognosis.

Materials And Methods: This retrospective study included patients with GD who underwent RAI therapy at the Second Affiliated Hospital of Anhui Medical University between October 2012 and October 2022. Spearman correlation and maximal information coefficient analyses were used, along with hierarchical clustering and Kaplan-Meier survival analysis.

Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production.

Secretion and folding are common bottlenecks in protein expression using eukaryotic systems, and engineering the secretory pathway to enhance host cell capabilities is a key strategy for improving protein secretion. However, secretion is a very complex process, making the identification of likely targets for engineering a formidable task. In this study, using glucose oxidase (GOX) expression in Pichia pastoris (Komagataella phaffii) as a model, we introduce a strategy called Hac1p-based inverse secretory pathway engineering (Hi-SPE).

"Using waste straw to prevent pollution": Enabling closed-loop design of chrome-free tanning agent with tanning-masking dual effect.

Abundant waste crop straw produced in China will be disposed, causing severe pollution to environment. Therefore, it is still a challenge for the cleaner treatment technology and high-value utilization. In this study, we extracted the biochemical fulvic acid (BFA) from crop straw, and then synthesized a novel biomass-based tanning agent (BAA) by coordination of BFA with Al.

Bionic polydopamine in-situ functionalized keratin double network gel with adsorption and self-adaptability: promoting uranium entrapment from seawater.

Adsorption behavior driven by surface charge is a challenge for uranyl extraction from seawater (UES), so it is attractive to design uranium adsorbents with charge and environment self-adaptability. In this paper, polyphenolamine (PPA) with a dopamine-like structure was synthesized by a one-step method using tannic acid (TA) and polyethyleneimine (PEI) as raw materials. The PPA with different isoelectric points are obtained by adjusting the ratios of TA and PEI, which enables the charge regulation of PPA within pH 6-9.

Analysis of high-risk factors for hepatocellular carcinoma after sustained virological suppression of chronic hepatitis B.

Chronic hepatitis B (CHB) patients who have sustained virological suppression (SVS) still develop hepatocellular carcinoma(HCC), and the risk factors for developing HCC in these patients are not fully understood. This study included a total of 5234 patients who achieved SVS. After SVS, the incidence rate of HCC was 1.

Rewiring the methanol assimilation pathway in the methylotrophic yeast Pichia pastoris for high-level production of erythritol.

Methanol, which is non-food competing, abundant, inexpensive, and potentially renewable, serves as an ideal alternative feedstock for biomanufacturing. Although engineered methylotrophic yeasts have successfully achieved gram-scale production of C2 (acetyl-CoA), C3 (pyruvate), and C6 (fructose-6-phosphate) building blocks from methanol, the production of C4-based (i.e.

Amino acid variants in the HLA-DQA1 and HLA-DQB1 molecules explain the major association of variants with relapse status in pediatric patients with steroid-sensitive nephrotic syndrome.

Background: Management of patients with steroid-sensitive nephrotic syndrome (SSNS) is challenging because of frequent relapses. Causal variants in the human leukocyte antigen (HLA) class II region that are associated with relapse remain undetermined.

Methods: We collected a cohort of East Asian individuals comprising 206 pediatric patients with SSNS and 435 healthy controls from Southwest China.

Multimodal machine learning-based model for differentiating nontuberculous mycobacteria from .

Objective: To develop and evaluate the effectiveness of multimodal machine learning approach for the differentiation of NTM from MTB.

Methods: The clinical data and CT images of 175 patients were retrospectively obtained. We established clinical data-based model, radiomics-based model, and multimodal (clinical plus radiomics) model gradually using 5 machine learning algorithms (Logistic, XGBoost, AdaBoost, RandomForest, and LightGBM).

Probability analysis of hepatocellular carcinoma in hepatitis patients in the gray zone.

Objective: To investigate the probability of hepatocellular carcinoma (HCC) in a large number of gray-zone (GZ) patients with chronic hepatitis B (CHB) in clinical practice.

Methods: The patients with CHB who were diagnosed and treated in our hospital from January 2013 to January 2023 were analyzed retrospectively.

Results: According to the different levels of HBeAg, ALT and HBV DNA, GZ patients were divided into four categories: (1) Gray zone A (GZ-A): HBeAg positive, normal ALT level, HBV DNA ≤ 10 IU/ml; (2) Gray zone B (GZ-B): HBeAg positive, ALT>ULN, HBV DNA ≤ 2 × 10 IU/ml; (3) Gray zone C (GZ-C): HBeAg negative, normal ALT level, HBV DNA ≥ 2 × 10 IU/ml; and (4) Gray zone D (GZ-D): HBeAg negative, ALT > ULN, serum HBV DNA ≤ 2 × 10 IU/ml.

Necessity of antiviral treatment for patients with chronic hepatitis B in the grey zone based on liver pathology analysis.

Objective: 28-55% of chronic hepatitis B (CHB) patients belong to the grey zone (GZ). By analyzing the pathological characteristics of the liver of patients in the GZ, this study clarified whether the patients in the GZ need anti-hepatitis B virus treatment.

Method: We reviewed 324 cases of liver pathology that underwent liver biopsy between 2011 and 2022.

Efficacy of Pharmacotherapies for Bulimia Nervosa: A Systematic Review and Meta-Analysis.

Objective: The main purpose was to evaluate the efficacy and tolerability of different medications used to treat bulimia nervosa (BN).

Methods: Randomized controlled trials (RCTs) were identified from published sources through searches in PubMed, Cochrane Library, Web of Science, and Embase from inception to November 2022. Primary outcomes were changes in the frequency of binge eating episodes and vomiting episodes from baseline to endpoint.

Evaluation of Safety and Efficacy of Amniotic Mesenchymal Stem Cells for POI in Animals.

The efficacy of human amniotic mesenchymal stem cell (hAMSC) ovarian injection in improving ovarian function in primary ovarian insufficiency (POI) patients has been shown in some reports. However, the safety and efficacy of hAMSC vein injection remains unclear. In this study, we evaluated the safety and efficacy of hAMSC intravenous injection in cynomolgus macaques and SD rats and provided evidence for clinical trials.

Molecular characteristics of circulating B cells and kidney cells at the single-cell level in special types of primary membranous nephropathy.

Background: Although primary membranous nephropathy (pMN) associated with podocyte autoantibodies (POS) is becoming well-known, the molecular characteristics of the specific type of pMN that is negative for podocyte autoantibodies (NEG) is still unclear.

Methods: We performed single-cell transcriptome sequencing and single-cell B cell receptor sequencing on circulating CD19 cells and kidney cells of a NEG paediatric patient with pMN. The single-cell datasets of POS patients and healthy control individuals were included for integrative analysis.

Efficacy of pharmacotherapies for bulimia nervosa: a systematic review and meta-analysis.

Objective: The main purpose was to evaluate the efficacy and tolerability of different medications used to treat bulimia nervosa (BN).

Methods: Randomized controlled trials (RCTs) were identified from published sources through searches in PubMed, Cochrane Library, Web of Science, and Embase from inception to November 2022. Primary outcomes were changes in the frequency of binge eating episodes and vomiting episodes from baseline to endpoint.

NAD(P)H:quinone Oxidoreductase 1 Is Involved in AZ-1-induced Apoptotic Effects in Nasopharyngeal Carcinoma TW01 Cells.

Background/aim: Current NPC treatment methods have improved the 5-year survival rates of patients; however, some patients do not benefit from the treatments. Therefore, the existing treatment methods or new drugs must be developed to improve the patient's prognosis. NAD (P)H:quinone oxidoreductase 1 (NQO1), an electron reductase highly expressed in various cancers, can convert aziridinyl-substituted quinone-derived compound into an alkylating agent, resulting in cell apoptosis.

Serum total bile acid levels assist in the prediction of acute intussusception with abdominal type Henoch-Schonlein purpura in children.

Background: The severe acute abdomen associated with Henoch-Schonlein purpura (HSP) is an acute intussusception (AI). There is no reliable specific marker for AI with abdominal-type HSP. The serum total bile acid (TBA) level is a new prognostic marker associated with the severity of intestinal inflammation.

Corrigendum: Clinical features and gene variation analysis of COQ8B nephropathy: Report of seven cases.

[This corrects the article DOI: 10.3389/fped.2022.

Guanfacine for the Treatment of Attention-Deficit Hyperactivity Disorder: An Updated Systematic Review and Meta-Analysis.

Non-stimulant guanfacine is a common second-line medication for attention-deficit hyperactivity disorder (ADHD). Numerous randomized controlled trials (RCTs) have explored the efficacy of guanfacine in ADHD treatment. This meta-analysis combined data from selected RCTs to analyze the efficacy and safety of guanfacine in treating ADHD.

Clinical features and gene variation analysis of COQ8B nephropathy: Report of seven cases.

Objective: COQ8B nephropathy is a relatively rare autosomal recessive kidney disease characterized by proteinuria and a progressive deterioration of renal function, eventually leading to end-stage renal disease (ESRD). The objective is to study the characteristics and correlation between the genotype and the clinical phenotype of COQ8B nephropathy.

Methods: This is a retrospective study focusing on the clinical characteristics of seven COQ8B nephropathy patients diagnosed by gene sequencing.

Microwave imaging of quasi-periodic pulsations at flare current sheet.

Quasi-periodic pulsations (QPPs) are frequently detected in solar and stellar flares, but the underlying physical mechanisms are still to be ascertained. Here, we show microwave QPPs during a solar flare originating from quasi-periodic magnetic reconnection at the flare current sheet. They appear as two vertically detached but closely related sources with the brighter ones located at flare loops and the weaker ones along the stretched current sheet.

PTC124 Rescues Nonsense Mutation of Two Tumor Suppressor Genes and to Repress HNSCC Cell Proliferation.

(1) Background: PTC124 (Ataluren) is an investigational drug for the treatment of nonsense mutation-mediated genetic diseases. With the exception of the tumor suppressor gene, there has been little research on cancers with nonsense mutation. By conducting a database search, we found that another two tumor suppressor genes, and , have a high nonsense mutation rate in head and neck squamous cell carcinoma (HNSCC).

New insights into the role of ribonuclease P protein subunit p30 from tumor to internal reference.

Ribonuclease P protein subunit p30 () is a highly conserved housekeeping gene that exists in many species and tissues throughout the three life kingdoms (archaea, bacteria, and eukaryotes). is closely related to a few types of tumors in human diseases but has a very stable transcription level in most cases. Based on this feature, increasing number of studies have used as an internal reference gene.

Polyguanine alleviated autoimmune hepatitis through regulation of macrophage receptor with collagenous structure and TLR4-TRIF-NF-κB signalling.

Autoimmune hepatitis (AIH) is a progressive and chronic inflammatory disease in the liver. MARCO is a surface receptor of macrophage involving in tissue inflammation and immune disorders. Moreover, polyguanine (PolyG) is considered to bind to macrophage receptor with collagenous structure (MARCO).

Whole-exome sequencing of a multicenter cohort identifies genetic changes associated with clinical phenotypes in pediatric nephrotic syndrome.

Understanding the association between the genetic and clinical phenotypes in children with nephrotic syndrome (NS) of different etiologies is critical for early clinical guidance. We employed whole-exome sequencing (WES) to detect monogenic causes of NS in a multicenter cohort of 637 patients. In this study, a genetic cause was identified in 30.