Synthesis and biological evaluation of sulfonamide derivatives containing imidazole moiety as ALK5 inhibitors.

Four series of sulfonamide derivatives (13a-b, 14a-d, 15a-b, and 16a-d) were synthesized and evaluated for their activin receptor-like kinase 5 (ALK5) inhibitory activities. Of these, compounds 13b (IC = 0.130 μM) and 15a (IC = 0.

Proteomics-Based Investigation of Different Live Prey Administered to Freshwater Dark Sleeper (): Examining the Effects on Glycolipids and Energy Metabolism.

Live prey is characterized by balanced rich nutrients and high palatability and is widely used for the seedling cultivation of freshwater dark sleeper () larvae. In this study, we evaluated the effects of four groups of paired feeding regimens (group C (), group L (), group H ( fry), and group M (mixed groups C, L, and H)) on glycolipid and energy metabolism in larvae. We observed that fatty acid synthase () and sterol-regulatory-element-binding protein-1 () mRNA levels were significantly lower in group H when compared to mRNA levels in the other three groups ( < 0.

Transcriptome analysis of immune response against Siniperca chuatsi rhabdovirus infection in mandarin fish Siniperca chuatsi.

As one of the piscine rhabdoviruses, Siniperca chuatsi rhabdovirus (SCRV) has caused considerable losses to mandarin fish aquaculture industry. RNA-seq, as efficient transcriptome research method, has been widely used to study the immune response of fish to pathogens. This study reported the effect of SCRV infection at 0, 24 and 60 hr on S.

A Gram-Negative Bacterial Secreted Protein Types Prediction Method Based on PSI-BLAST Profile.

Prediction of secreted protein types based solely on sequence data remains to be a challenging problem. In this study, we extract the long-range correlation information and linear correlation information from position-specific score matrix (PSSM). A total of 6800 features are extracted at 17 different gaps; then, 309 features are selected by a filter feature selection method based on the training set.

Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism.

Subclinical hypothyroidism (SCH) is becoming a global health problem due to its increasing prevalence and potential deleterious effects. However, the molecular mechanisms underlying the lipid metabolic disorders in SCH have not been fully clarified. Additionally, progress in elucidating the exact pathogenesis of SCH has been hampered by the lack of optimized mouse models.

A protein structural classes prediction method based on PSI-BLAST profile.

Knowledge of protein structural classes plays an important role in understanding protein folding patterns. Prediction of protein structural class based solely on sequence data remains to be a challenging problem. In this study, we extract the long-range correlation information and linear correlation information from position-specific score matrix (PSSM).

A protein structural classes prediction method based on predicted secondary structure and PSI-BLAST profile.

Knowledge of protein secondary structural classes plays an important role in understanding protein folding patterns. In this paper, 25 features based on position-specific scoring matrices are selected to reflect evolutionary information. In combination with other 11 rational features based on predicted protein secondary structure sequences proposed by the previous researchers, a 36-dimensional representation feature vector is presented to predict protein secondary structural classes for low-similarity sequences.

A simple k-word interval method for phylogenetic analysis of DNA sequences.

In this study, the normalized k-word average interval distance is proposed to extract phylogenetic information from DNA sequences. The phylogenetic trees of 30 mammalian species based on Euclidean distance measure are reconstructed with k ranging from 2 to 9. Comparison of our results with other methods shows that our method is efficient and powerful for phylogenetic analysis.

A novel protein structural classes prediction method based on predicted secondary structure.

Knowledge of structural classes plays an important role in understanding protein folding patterns. In this paper, features based on the predicted secondary structure sequence and the corresponding E-H sequence are extracted. Then, an 11-dimensional feature vector is selected based on a wrapper feature selection algorithm and a support vector machine (SVM).

Comparative study on the toxicity of pyrethroids, α-cypermethrin and deltamethrin to Ceriodaphnia dubia.

Two synthetic pyrethroid pesticides, α-cypermethrin and deltamethrin were investigated as potential toxic contaminants. The acute and chronic bioassays were conducted using Ceriodaphnia dubia. The toxicity of α-cypermethrin and deltamethrin to C.

High-accuracy prediction of protein structural class for low-similarity sequences based on predicted secondary structure.

Information on the structural classes of proteins has been proven to be important in many fields of bioinformatics. Prediction of protein structural class for low-similarity sequences is a challenge problem. In this study, 11 features (including 8 re-used features and 3 newly-designed features) are rationally utilized to reflect the general contents and spatial arrangements of the secondary structural elements of a given protein sequence.

A simple feature representation vector for phylogenetic analysis of DNA sequences.

In this study, a simple 4k-dimension feature representation vector is proposed to reconstruct phylogenetic trees, where k is the length of a word. The vector is composed of elements which characterize the relative difference of biological sequence from sequence generated by an independent random process. In addition, the variance of a vector which is obtained by averaging every column of feature representation matrix is employed to determine appropriate word length.

Risk to humans of consuming metals in anchovy (Coilia sp.) from the Yangtze River Delta.

Concentrations of metals were determined in four species of anchovy (Coilia sp.) from the Yangtze River, Taihu Lake, and Hongze Lake in Jiangsu Province, China. Concentrations of Cr in anchovy fish muscle ranged from 2.

RETARDED PALEA1 controls palea development and floral zygomorphy in rice.

Poaceae, one of the largest flowering plant families in angiosperms, evolved distinct inflorescence and flower morphology diverging from eudicots and other monocots. However, the mechanism underlying the specification of flower morphology in grasses remains unclear. Here we show that floral zygomorphy along the lemma-palea axis in rice (Oryza sativa) is partially or indirectly determined by the CYCLOIDEA (CYC)-like homolog RETARDED PALEA1 (REP1), which regulates palea identity and development.

Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi.

In order to develop cryopreservation techniques for long-term preserving the sperm of Mandarin fish Siniperca chuatsi, we examined the effects of various extender and cryopreservation on post-thaw motility. We found the optimal freezing procedures for the Mandarin fish sperm is diluting the semen in D-15 extender, chilling it to 4 degrees C, adding ME2SO to a final concentration of 10% (v/v), then transferring the semen in cryotubes, holding the cryotubes for 10 min at 6 cm (about -180 degrees C) above the surface of liquid nitrogen, for 5 min on the surface of liquid nitrogen, and finally plunged into liquid nitrogen. After thawed at 37 degrees C for 60s, the sperm had the highest post-thaw motility (96.

[Genetic analysis and mapping of the rice leafy-hull mutant Oslh].

Oslh (lh=leafy hull), in the japonica cultivar 9522 background, a mutant of Oryza sativa L. spp. japonica cv.