Analysis of spatial and temporal distribution of grassland yield under grassland ecological subsidy policy.

The Three River Headwater Region is an ecologically sensitive and fragile area. The analysis of long-term grassland yield changes, as well as the impact of climate and topography on grassland yield is of great significance to grassland ecological protection and the implementation of ecological subsidy policy in the Three River Headwater Region. Based on the grassland yield data from 2011 to 2016, this paper analyzed the spatial and temporal distribution of grassland yield and variations in grass production since the implementation of the grassland ecological subsidy policy in Qinghai Province by using linear regression analysis, coefficient of variation, and biased correlation analysis.

Evaluation of insect-tea quality and screening of tea-producing insects through non-targeted metabolomics and gut microbiota analysis.

Insect tea is a unique beverage in China, jointly produced by tea-producing insects and their host plants. In this study, gut microbiota and non-targeted metabolomics analysis were conducted on insect tea produced by different tea-producing insects (Aglossa dimidiata and Martyria xeraula) feeding on the same host plant(Litsea coreana). Significant differences in gut microbiota were observed between the two tea-producing insects.

Myocardial pyruvate dehydrogenase kinase 4 drives sex-specific cardiac responses to endotoxemia.

Males often experience worse cardiac outcomes than females in sepsis. This study identified pyruvate dehydrogenase kinase 4 (PDK4) as a key mediator of this disparity. PDK4 regulates glucose utilization by inhibiting pyruvate dehydrogenase (PDH) in mitochondria.

Uncovering key markers and therapeutic targets for renal fibrosis in diabetic kidney disease through bulk and single-cell RNA sequencing.

Background: Diabetic kidney disease (DKD) is the major cause of chronic kidney failure, with tubulointerstitial fibrosis playing a crucial role in disease development. Identifying fibrosis-related genes is crucial for improving diagnosis and developing novel therapies due to the necessity for early detection and effective treatments.

Methods: Genes associated with fibrosis were identified by WGCNA, and a FibrosisScore model was constructed based on ssGSEA scores from two DKD datasets.

The Interaction Among Rhizosphere Soil Nutrients, Metabolites, and Microbes Determines the Productivity of Perennial Cultivated Grassland in Qinghai-Tibet Plateau.

The rhizosphere, as the primary site of plant-soil -microbe interactions, plays an important role in plant productivity. The influence of plant species on rhizosphere soil properties and how this influence shapes the productivity of grassland ecosystems remains poorly understood. Therefore, this study analyzed the changes in bacterial community structure and metabolites in the rhizosphere soil of perennial cultivated grassland.

Effects of planting patterns on physicochemical properties, metabolites and microbial community structure of rhizosphere soil in perennial cultivated grassland.

Establishing perennial cultivated grasslands on the Qinghai-Tibet Plateau helps address the seasonal imbalance of forage resources and supports the restoration of degraded grasslands. The most common planting patterns-monocropping and mixed cropping-are well-studied in terms of vegetation structure, productivity, and soil nutrients. Despite their significance, the influence of prolonged planting practices on underground soil microbial communities and metabolites has often been neglected.

Clinical implement of Probe-Capture Metagenomics in sepsis patients: A multicentre and prospective study.

Background: Accurate pathogen identification is critical for managing sepsis. However, traditional microbiological methods are time-consuming and exhibit limited sensitivity, particularly with blood samples. Metagenomic sequencing of plasma or whole blood was highly affected by the proportion of host nucleic acid.

Phylogenetic and Codon Usage Bias Analysis Based on mt-DNA of (Coleoptera: Melolonthinae) and Its Neighboring Species.

: In order to determine the basic structural characteristics of the mitochondrial genome of and explore its phylogenetic status, as well as to understand the codon usage bias of Melolonthinae species, the next-generation sequencing was used to obtain the mitochondrial genome sequence of . : Combined with 121 sequences of Scarabaeidae downloaded from GeneBank, a phylogenetic tree of the family was constructed using PhyloSuite v 1.2.

Research on optimization of emergency supplies customs clearance strategy from the perspective of public health security based on Chinese trade data.

Background: This study is significant for improving the accuracy of Customs' cross-border supervision of emergency supplies and ensuring the timely clearance of these essential goods.

Methods: To ensure both the convenience and security of Customs oversight regarding emergency supplies, this study first systematically collects and organizes representative data on the import and export trade of these supplies. Proposed an enhanced Recency Frequency Monetary (RFM) cluster analysis model, building on the principles of k-means clustering.

[Response of Microbiological Properties to Short-term Nitrogen Addition in Perennial Alpine Cultivated Grassland].

Removing nitrogen limitation is necessary to increase plant productivity in alpine grasslands. A short-term nitrogen addition experiment was conducted to understand the effects of nitrogen addition on the soil physicochemical properties and microbial community structure of perennial alpine cultivated grassland in the region around Qinghai Lake. From June to August 2022, four N application gradients （T0： 0 kg·hm·a, T1： 22.

Identification of crucial genes and possible molecular pathways associated with active vitamin D intervention in diabetic kidney disease.

Background: A significant cause of advanced renal failure is diabetic nephropathy (DKD), with few treatment options available. Calcitriol shows potential in addressing fibrosis related to DKD, though its molecular mechanisms remain poorly understood. This research seeks to pinpoint the crucial genes and pathways influenced by calcitriol within the scope of DKD-related fibrosis.

Cardiac Localized Polycystin-2 in the Natriuretic Peptide Signaling Pathway and Hypertension.

Key Points: Cardiac localized polycystin facilitates natriuretic peptide signaling pathways. Hypertension associated with autosomal dominant polycystic kidney disease may arise from impaired cardiac natriuretic peptide signaling.

Background: Hypertension is seen in 70% of patients with autosomal dominant polycystic kidney disease by age of 30 years before decline in kidney function.

Omicron variant and pulmonary involvements: a chest imaging analysis in asymptomatic and mild COVID-19.

Objectives: To identify clinical characteristics and risk factors for pulmonary involvements in asymptomatic and mildly symptomatic patients infected with SARS-CoV-2 Omicron variant by chest imaging analysis.

Methods: Detailed data and chest computed tomography (CT) imaging features were retrospectively analyzed from asymptomatic and mildly symptomatic patients infected with Omicron between 24 April and 10 May 2022. We scored chest CT imaging features and categorized the patients into obvious pulmonary involvements (OPI) (score > 2) and not obvious pulmonary involvements (NOPI) (score ≤ 2) groups based on the median score.

Vascular Aging in Ischemic Stroke.

Cellular senescence, a permanent halt in cell division due to stress, spurs functional and structural changes, contributing to vascular aging characterized by endothelial dysfunction and vascular remodeling. This process raises the risk of ischemic stroke (IS) in older individuals, with its mechanisms still not completely understood despite ongoing research efforts. In this review, we have analyzed the impact of vascular aging on increasing susceptibility and exacerbating the pathology of IS.

[Effects of Different Forms of Nitrogen Addition on Soil Physical and Chemical Properties and Microbial Community Structure of Perennial Alpine Cultivated Grassland].

This study aimed to investigate the impact of different nitrogen forms on soil physicochemical properties and microbial community structure in perennial alpine cultivated grasslands, in order to provide scientific basis for developing nitrogen addition strategies for perennial alpine cultivated grasslands. In June 2022, a 4-year-old Qinghai grassland mixed with Qinghai and Qinghai was established at the Bakatai Farm in Gonghe County, Hainan Tibetan Autonomous Prefecture, Qinghai Province. The study was conducted without fertilization as a control (CK), and three different forms of nitrogen treatments were set up, namely, U:urea (amide nitrogen), A:ammonium sulfate (ammonium nitrogen), and N:calcium nitrate (nitrate nitrogen); the nitrogen application rate for each treatment was 67.

Utilization of the genetically encoded calcium indicator Salsa6F in cardiac applications.

Calcium signaling is a critical process required for cellular mechanisms such as cardiomyocyte contraction. The inability of the cell to properly activate or regulate calcium signaling can lead to contractile dysfunction. In isolated cardiomyocytes, calcium signaling has been primarily studied using calcium fluorescent dyes, however these dyes have limited applicability to whole organs.

[Construction and verification of a nomogram of factors influencing the risk of death in patient with sepsis-associated thrombocytopenia].

Objective: To construct a nomogram prediction model for predicting the risk of death in patients with sepsis-associated thrombocytopenia (SAT) in intensive care unit (ICU) for early indentification and active intervention.

Methods: Clinical data of SAT patients admitted to ICU of the First Affiliated Hospital of Nanjing Medical University from December 2019 to August 2021 were retrospectively collected, including demographic data, laboratory indicators, etc. According to the prognosis at 28 days, the patients were divided into the death group and the survival group, and the differences of clinical variables between the two groups were compared.

Cardiac Localized Polycystin-2 plays a Functional Role in Natriuretic Peptide Production and its Absence Contributes to Hypertension.

Cardiovascular complications are the most common cause of mortality in patients with autosomal dominant polycystic kidney disease (ADPKD). Hypertension is seen in 70% of patients by the age of 30 prior to decline in kidney function. The natriuretic peptides (NPs), atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), are released by cardiomyocytes in response to membrane stretch, increasing urinary excretion of sodium and water.

Utilization of the genetically encoded calcium indicator Salsa6F in cardiac applications.

Calcium signaling is a critical process required for cellular mechanisms such as cardiac contractility. The inability of the cell to properly activate or regulate calcium signaling can lead to contractile dysfunction. In isolated cardiomyocytes, calcium signaling has been primarily studied using calcium fluorescent dyes, however these dyes have limited applicability to whole organs.

Low-intensity pulsed ultrasound of different intensities differently affects myocardial ischemia/reperfusion injury by modulating cardiac oxidative stress and inflammatory reaction.

Introduction: The prevalence of ischemic heart disease has reached pandemic levels worldwide. Early revascularization is currently the most effective therapy for ischemic heart diseases but paradoxically induces myocardial ischemia/reperfusion (MI/R) injury. Cardiac inflammatory reaction and oxidative stress are primarily involved in the pathology of MI/R injury.

Cascading effects of seed-stem-individual spatial patterns along a grazing gradient.

Studying the seed trait-stem trait-individual spatial pattern system is helpful for understanding the developmental direction of plant dynamics and populations under grazing disturbance as well as the antagonistic relationship between animals and plants, but few systematic analyses of this spatial pattern system have been carried out. is the dominant species in alpine grasslands. We studied seed traits and their relationship with reproductive individuals, the relationship between reproductive and vegetative stems, and the weights and spatial patterns of reproductive and nonreproductive individuals under four grazing treatments: no grazing (control), light grazing, moderate grazing and heavy grazing.

Herbivore assemblages affect soil microbial communities by altering root biomass and available nutrients in an alpine meadow.

Three different herbivore grazing assemblages, namely, yak grazing (YG), Tibetan sheep grazing (SG) and yak and Tibetan sheep co-grazing (MG), are practiced in alpine meadows on the Qinghai-Tibetan Plateau (QTP), but the effects of the different herbivore assemblages on soil microbes are relatively unknown. The microbial community plays an important role in the functional stability of alpine grassland ecosystems. Therefore, it is important to understand how the microbial community structure of grassland ecosystems changes under different herbivore grazing assemblages to ensure their sustainable development.