Core Transcriptional Regulatory Circuit-Regulated IGF2BP3 Stabilizes E2F2 mRNA via m6A Modification in Neuroblastoma.

Neuroblastoma (NB) is a pediatric tumor with diverse outcomes and unknown underlying mechanisms. The core transcriptional regulatory circuit (CRC) and N6-methyladenosine (m6A) are key factors that control cell identity and fate. IGF2BP3 is an m6A reader protein that is transcriptionally regulated by CRC transcription factors (TFs).

Optical Vernier effect-based demodulation system for fiber-optic sensors: an AWG and a neural network-based perspective.

Accurate demodulation of fiber-optic sensors is crucial for real-world engineering applications in monitoring and control. This paper presents a method that integrates neural networks with arrayed waveguide gratings (AWGs) for the demodulation of fiber-optic sensors based on the Vernier effect and a novel, to our knowledge, Fabry-Pérot (FP) strain sensor structure. Conventional demodulation techniques exhibit limited generalization capabilities, whereas neural networks can establish complex nonlinear mappings.

Fiber-optic Fabry-Perot pressure sensor with the Vernier effect based on dynamic adjustment of the cavity length method.

This paper proposes and validates a fiber-optic pressure sensor based on a Fabry-Perot interferometer, which enhances sensitivity through the Vernier effect generated by concatenating parallel cavities. The sensor consists of a sensing cavity and a reference cavity. The sensing cavity was fabricated by fusing a segment of short multimode fiber (MMF) to a single-mode fiber (SMF), followed by the fusion of a segment of capillary glass tubing (HST1) to the end of the MMF, and coating the end of HST1 with a polydimethylsiloxane film.

SD49-7 attenuates cardiac hypertrophy by upregulation of S100B through KDM4A inhibition.

Cardiac hypertrophy is a major contributor to heart failure, yet therapeutic options remain limited. Emerging evidence suggests that upregulated expression of histone lysine-specific demethylase 4A (KDM4A) in patients with cardiac hypertrophy may represent a significant, albeit underappreciated, disease risk factor; however, the underlying mechanism and specific downstream pathway remain unclear. In this study, we investigated the therapeutic effect of SD49-7, a specific KDM4A inhibitor, on cardiac hypertrophy and its underlying mechanism, utilizing both in vivo and in vitro models.

Global Research Progress of Mitochondria and Hypoxic-Ischemic Encephalopathy: A Comprehensive Bibliometric Analysis.

BackgroundHypoxic-ischemic encephalopathy is a serious neurologic condition caused by reduced blood and oxygen supply to the brain, typically occurring during birth. Mitochondria play a crucial role in the pathophysiological mechanisms of hypoxic-ischemic encephalopathy, yet a comprehensive bibliometric analysis of this research area is lacking. This study aims to identify research hotspots and trends related to mitochondria and hypoxic-ischemic encephalopathy through a detailed bibliometric analysis.

Metabolic reprogramming of NK cells drives anti-tumor immunity.

Cellular metabolism critically controls immune cell functionality, necessitating dynamic metabolic adaptation to sustain effector responses under microenvironmental fluctuations. Natural killer (NK) cells, innate lymphocytes essential for tumor immunosurveillance, exhibit functional plasticity intrinsically linked to metabolic reprogramming. While NK cell-based immunotherapy show promise due to MHC-independent cytotoxic, their clinical efficacy diverges markedly between hematological and solid tumor-a disparity rooted in tumor microenvironment (TME) driven immunosuppression.

Differences in the gut microbiota of the black-faced spoonbill () across different regions in China.

The black-faced spoonbill () is a critically endangered, first-class protected bird species in China, and its gut microbiota is thought to play a crucial role in the bird's ecological adaptability. However, regional variations in the gut microbiota of this species remain poorly understood. We aimed to investigate how the gut microbiota of differs across distinct regions in China to identify key factors influencing its composition and function.

Analysis and characterization of extrachromosomal circular DNA in prostate cancer: Potential biomarker discovery from urine, plasma, and tumor samples.

Extrachromosomal circular DNA (eccDNA) may contribute to genomic rearrangements and tumor heterogeneity, playing a role in cancer development and progression. This study evaluates eccDNA as a biomarker for prostate cancer by characterizing its profiles in urine, plasma, and tumor tissues from patients at different disease stages. We studied 49 prostate cancer patients (23 early-stage; 26 late-stage, including 19 with metastasis), 23 patients with prostatitis, and 21 healthy individuals.

High-sensitivity strain sensor based on a Fabry-Pérot interferometer with a tapered capillary structure.

A high-sensitivity strain sensor based on a Fabry-Pérot interferometer (FPI) with a tapered capillary structure is proposed and experimentally demonstrated. The sensor is designed to enhance strain responsiveness while minimizing temperature cross-sensitivity, making it suitable for precision strain measurements. It is fabricated by fusing a segment of capillary glass tube between two single-mode fibers using a cost-effective and simple method.

Recharging for the future: how micro-breaks shape work prospection among Chinese employees.

Despite a surge of research on micro-breaks in recent years, the interactional nature of this recovery behavior during working hours still needs to be explored. Drawing on the Social Exchange Theory and Conservation of Resources Theory, first, we found from a study that included three online vignette experiments (Study 1,  = 551) that three micro-break support conditions (management support, supervisor norms, and coworker norms) enhanced the positive relationship between micro-breaks and three affective relationships (affective organizational commitment, affective supervisor-subordinate guanxi, and friendship prevalence), respectively. Second, a two-wave time-lag survey study (Study 2,  = 208) replicated the findings of Study 1 and verified the positive relationship between micro-breaks and positive affective work prospection and the mediating role of affective relationships therein and the moderating effect of micro-break support.

Analysis of the Primary Pathogenic Bacteria in Abscess Disease of Musk Deer Using Metagenomic Approaches.

Abscesses are among the diseases affecting the survival of captive musk deer and are difficult to identify in their early stages. In this study, metagenomic sequencing, 16S rRNA sequencing, and paraffin sectioning were used to analyze the microbiota in the abscess musk deer pus group (AMP), abscess musk deer oral group (AMO), and healthy musk deer oral group (HMO) to compare the differences in microbiota in musk deer. By detecting differences in the oral microbiota through throat swabs, we aimed to monitor the early onset of abscess disease to facilitate timely intervention and treatment.

Integrating CORUM and co-fractionation mass spectrometry to create enhanced benchmarks for protein complex predictions.

Co-fractionation mass spectrometry (CFMS) enables the discovery of protein complexes and the systems-level analysis of multimer dynamics that facilitate responses to environmental and developmental conditions. A major challenge in CFMS data analysis, and omics approaches in general, is the development of reliable benchmarks for accurate evaluation of prediction methods. CORUM is commonly used as a source of benchmark complexes for protein complex composition predictions; however, its assumption of fully assembled subunit pools often conflicts with size exclusion chromatography (SEC) and interaction predictions from CFMS experiments.

Aging-related impairment of neurogenic chloride secretion in human colon mucosa.

Background: lderly individuals are more susceptible to chronic constipation, which may be linked to imbalanced mucosa secretion and absorption. Our research aims to explore the age-related alterations in epithelial chloride secretion within the human colon.

Methods: Colonic mucosal tissues were obtained from 9 young patients (aged 28-35 years), 10 middle-aged patients (aged 48-56 years), 10 elderly patients without constipation (aged 66-75 years), and 12 elderly patients with constipation (aged 65-78 years) who underwent surgery for colonic carcinoma.

A high-resolution model of gene expression during Gossypium hirsutum (cotton) fiber development.

Background: Cotton fiber development relies on complex and intricate biological processes to transform newly differentiated fiber initials into the mature, extravagantly elongated cellulosic cells that are the foundation of this economically important cash crop. Here we extend previous research into cotton fiber development by employing controlled conditions to minimize variability and utilizing time-series sampling and analyses to capture daily transcriptomic changes from early elongation through the early stages of secondary wall synthesis (6 to 24 days post anthesis; DPA).

Results: A majority of genes are expressed in fiber, largely partitioned into two major coexpression modules that represent genes whose expression generally increases or decreases during development.

Role of declined electrogenic Na/HCO cotransporter NBCe1 in mucus barrier impairment and colonic inflammation.

Background: Electrogenic Na/HCO co-transporter 1 (NBCe1) plays a pivotal role in epithelial bicarbonate transport involved in the maintenance of the intestinal mucus barrier. However, the specific role of NBCe1 in colitis remains unknown.

Methods: NBCe1 was identified by bioinformatics analysis methods including GO/KEGG/GSEA, protein-protein interaction (PPI) network analysis, immune infiltration analysis, and Mendelian randomization (MR) analysis.

Risk Factors of In-Hospital Venous Thromboembolism and Prognosis After Emergent Ventral Hernia Repair.

The risk factors and association of venous thromboembolism (VTE) following emergent ventral hernia repair (EVHR) remains uncertain. This aim of the study aims was to establish the predictors of VTE after EVHR and its influence on the long-term outcomes. A total of 2093 patients from the MIMIC-IV database who underwent EVHR were recruited.

Thirty years of Ca spark research: digital principle of cell signaling unveiled.

Calcium ions (Ca) are an archetypical and most versatile second messenger in virtually all cell types. Inspired by the discovery of Ca sparks in the 1990s, vibrant research over the last three decades has unveiled a constellation of Ca microdomains as elementary events of Ca signaling and, more importantly, a digital-analog dualism as the system design principle of Ca signaling. In this brief review, we present a sketchy summary on advances in the field of sparkology, and discuss how the digital subsystem can fulfill physiological roles otherwise impossible for any analog system.

Daily glycome and transcriptome profiling reveals polysaccharide structures and correlated glycosyltransferases critical for cotton fiber growth.

Cotton fiber is the most valuable naturally available material for the textile industry and the fiber length and strength are key determinants of its quality. Dynamic changes in the pectin, xyloglucan, xylan, and cellulose polysaccharide epitope content during fiber growth contribute to complex remodeling of fiber cell wall (CW) and quality. Detailed knowledge about polysaccharide compositional and structural alteration in the fiber during fiber elongation and strengthening is important to understand the molecular dynamics of fiber development and improve its quality.

Clinical Characteristics and Diagnosis of Nonaccelerating MDS/MPN-U Patient with 5q- Karyotype.

Background: The aim of the study was to improve the clinical cognition of nonaccelerating myelodysplastic/myeloproliferative neoplasms-unclassifiable (MDS/MPN-U) with 5q- karyotype and to avoid misdiagnosis or delayed diagnosis.

Methods: The clinical manifestations and laboratory results of a patient with nonaccelerating MDS/MPN-U with 5q- karyotype were analyzed, and related literature was reviewed.

Results: The patient was admitted to hospital mainly due to chest tightness and shortness of breath, aggravated for 4 days.

Two new phenolic glycosides from the roots of (L.) Goldblatt & Mabb and their cytotoxic activity.

Two new phenolic glycosides, Diplostephioside D () and Diplostephioside E () together with ten known phenols (-) were isolated from the dried roots of (L.) Goldblatt & Mabb. Among them, compounds - have not been reported previously from the family Iridaceae.

Experimental demonstration of topological bounds in quantum metrology.

Quantum metrology is deeply connected to quantum geometry, through the fundamental notion of quantum Fisher information. Inspired by advances in topological matter, it was recently suggested that the Berry curvature and Chern numbers of band structures can dictate strict lower bounds on metrological properties, hence establishing a strong connection between topology and quantum metrology. In this work, we provide a first experimental verification of such topological bounds, by performing optimal quantum multi-parameter estimation and achieving the best possible measurement precision.

Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions.

Advancements in human-engineered heart tissue have enhanced the understanding of cardiac cellular alteration. Nevertheless, a human model simulating pathological remodeling following myocardial infarction for therapeutic development remains essential. Here we develop an engineered model of myocardial repair that replicates the phased remodeling process, including hypoxic stress, fibrosis, and electrophysiological dysfunction.

Efficient degradation and enhanced anticomplementary activity of Belamcanda chinensis (L.) DC. polysaccharides via trifluoroacetic acid treatment with different degrees.

The degradation of Belamcanda chinensis (L.) DC. polysaccharides was carried out by five concentrations of trifluoroacetic acid (TFA) (1-5 mol/L), and their physicochemical properties, degradation kinetics and anticomplementary activity were investigated.