Exploring the Diversity and Distribution of Medico-Veterinary Fungal Diseases in Africa: Harnessing a Multisectoral One Health Strategy for Cost-Effective Prevention and Preparedness.

The diversity and distribution of medical and veterinary-relevant fungal diseases in Africa underscore the critical need for a multisectoral One Health strategy to enhance cost-effective preparedness and prevention. This review explores the geographic spread and epidemiology of key medical and veterinary fungi, including , , , , , , , , , , and . Evidence indicates that many of these infections remain underdiagnosed and underreported, especially in vulnerable immunocompromised populations, due to limited surveillance, diagnostic capacity, and awareness.

Evaluating cholesterol de novo synthesis biomarkers: a systematic review and meta-analysis of cancer prognosis and clinical outcomes.

Background: While systemic cholesterol levels are generally associated with cancer risk and progression in various tumors, studies of cholesterol de novo synthesis by cancer cells in various tumor settings were limited. This meta-analysis aims to provide a comprehensive understanding of the role of cholesterol de novo synthesis pathway in cancer, focusing on key markers related with this metabolic reprogramming in cancer tissues.

Methods: A systematic review and meta-analysis were conducted using data from multiple databases, including PubMed, EMBASE, and Cochrane Library.

Diversity and distribution of viral zoonosis in Africa.

Viral zoonoses pose a significant public health challenge in Africa, characterized by a diverse array of pathogens transmitted from animals to humans. This review maps the geographic distribution of zoonotic viral diseases in Africa, including rodent-borne, bat-borne, tick-borne, mosquito-borne, and sandfly-associated viral zoonoses, along with those transmitted via fecal-oral and respiratory routes. This review informs surveillance, resource allocation, and One Health strategies by mapping zoonotic viral disease risks across Africa based on geographic existence.

Mapping of the Antinuclear Autoantibodies in Sudanese patients infected with Human Immunodeficiency Virus after HAART receiving: A cross-sectional study.

It is important to highlight that infection with the human immunodeficiency virus (HIV) may trigger chronic inflammation in people living with HIV/AIDS (PLWHA), impacting to the key immune cells such B and T lymphocytes, leading to development of autoantibodies which might be potential to developed autoimmune phenomenon. This study represent the first investigation in Sudan designed to evaluate and determine the prevalence of Antinuclear autoantibody (ANA) among people living with HIV/AIDS by systematically charaterizing ANA staining patterns; distribution, frequency and their correlation with estimated endpoint titers and interplay of age -sex specific ANA patterns in the context of HIV infection post antiretroviral therapy era. Using Serum samples of one hundred and sixteen (116) HIV-infected patients admitted to two major Voluntary Testing and Counseling (VTC), of the HIV clinical centers at the Military Hospital and Bahri Hospital in Khartoum, Sudan, have been assessed for ANA screening.

Comprehensive mitochondrial genomics of from Sudan: insights into genetic diversity, evolutionary dynamics, and host adaptation.

Introduction: This study presents a comprehensive analysis of the complete mitochondrial genomes of isolated from cattle, sheep, and goats in Sudan, aiming to provide new insights into genetic diversity, evolutionary dynamics, and host adaptation.

Methods: Mitochondrial genomes were sequenced using high-throughput Illumina MiSeq technology, yielding sequences of 14,483 bp, slightly longer than the reference genome (14,478 bp). A sliding window analysis was conducted to assess nucleotide diversity, and phylogenetic analyses were performed using complete mitochondrial sequences, including and excluding non-coding regions.

Genomic Evolution and Phylodynamics of the Species (Marburg and Ravn Viruses) to Understand Viral Adaptation and Marburg Virus Disease's Transmission Dynamics.

In this review, we investigated the genetic diversity and evolutionary dynamics of the species that includes both Marburg virus (MARV) and Ravn virus (RAVV). Using sequence data from natural reservoir hosts and human cases reported during outbreaks, we conducted comprehensive analyses to explore the genetic variability, constructing haplotype networks at both the genome and gene levels to elucidate the viral dynamics and evolutionary pathways. Our results revealed distinct evolutionary trajectories for MARV and RAVV, with MARV exhibiting higher adaptability across different ecological regions.

A Case Report of Membranoproliferative Glomerulonephritis: Infection-Related or Immune-Related?

Membranoproliferative glomerulonephritis (MPGN) has previously been used as an umbrella term to describe a spectrum of hypocomplementemic glomerular diseases, which are rare causes of end stage kidney disease (ESKD). We present a 22-year-old man with a well-established medical history who had been complaining of 4 days of frothy dark urine, bilateral lower limb swelling, and puffiness on his face. For a month before his presentation, he had many bilateral skin lesions on his lower limbs that were leaking pus.

Undetected circulation of major arboviruses in West Sudan: urging for institutionalizing multisectoral one health strategy for the preparedness, prevention, and control of zoonotic arboviral diseases.

Objectives: Arboviruses pose a significant global health challenge. This study investigated the seroprevalence of major human arboviral infections, including yellow fever (YFV), dengue (DENV), Crimean-Congo hemorrhagic fever (CCHF), Rift Valley fever (RVF), West Nile virus (WNV), and chikungunya (CHIK), in Darfur region from September to December 2018. ELISA-IgM was used to detect antibodies.

Assessment of the COVID-19 Surveillance System in Sudan: Performance, Limitations, and Recommendations.

Assessing the COVID-19 surveillance system is vital. It identifies cases swiftly and is crucial in curbing COVID-19 spread, especially among vulnerable groups. Public health surveillance collects, analyzes, and shares data systematically, informing actions to lessen disease impact.

Severe coinfection of dengue and malaria: A case report.

Key Clinical Message: In countries like Sudan, where several infectious diseases are prevalent, health care providers should not be satisfied with initial detection of a single pathogen and whenever it is feasible, they should investigate coinfections. Infections with high mortality or severe morbidity should be prioritized during the differential diagnosis particularly for diseases with similar clinical manifestations to reduce the death and disability rates. However, this requires substantial improvement in the diagnostic capacity.

Intra- and interspecies variation and population dynamics of Fasciola gigantica among ruminants in Sudan.

Fasciola gigantica is a widespread parasite that causes neglected disease in livestock worldwide. Its high transmissibility and dispersion are attributed to its ability to infect intermediate snail hosts and adapt to various mammalian definitive hosts. This study investigated the variation and population dynamics of F.

Delayed cerebellar ataxia induced by malaria: A rare complication.

Key Clinical Message: In endemic areas, malaria-induced cerebellar ataxia should be suspected in patients presenting with neurological disorders including slurred speech, tremors, and a sense of imbalance and dizziness while walking. Healthcare providers should be aware to properly investigate and early detect and manage infections associated with the development of cerebellar ataxia to improve the case management and clinical outcome cost-effectively.

Abstract: Here, we report the clinical manifestations, investigations, and outcomes of a patient developed delayed cerebellar ataxia following a malaria infection: an unusual complication of the disease.

Molecular detection of Epstein-Barr virus among Sudanese patients diagnosed with Hashimoto's thyroiditis.

Objectives: Hashimoto's thyroiditis (HT) is the most common cause of hypothyroidism. The exact mechanism initiating the development of HT is not yet clear. This study aimed to investigate the correlation between HT and the presence of Epstein-Barr virus (EBV) in a Sudanese population.

Detection of Nonsynonymous Single Variants in Human HLA-DRB1 Exon 2 Associated with Renal Transplant Rejection.

: HLA-DRB1 is the most polymorphic gene in the human leukocyte antigen (HLA) class II, and exon 2 is critical because it encodes antigen-binding sites. This study aimed to detect functional or marker genetic variants of HLA-DRB1 exon 2 in renal transplant recipients (acceptance and rejection) using Sanger sequencing. : This hospital-based case-control study collected samples from two hospitals over seven months.

Knowledge and attitudes towards cervical cancer prevention among women in Khartoum state, Sudan.

Background: Cervical cancer is considered the third leading cause of death among women worldwide, and human papillomavirus was identified as a major causative agent for developing cervical cancer.

Objectives: This study aimed to assess the knowledge and attitudes towards cervical cancer prevention among women in Khartoum state, Sudan.

Design: A community-based cross-sectional study implemented in Khartoum state, Sudan, from 1 August 2020 to 1 September 2020.

Genetic variation and demographic history of Sudan desert sheep reveal two diversified lineages.

More than 400 million sheep are raised on the African continent, the majority of which are indigenous and are primarily reared for sustenance. They have effectively adapted to various climatic and production environments, surviving and flourishing. The genetic relationships among these sheep populations remain understudied.

Guillain-Barre syndrome associated with COVID-19 infection: A case series.

In this communication, we reported a series of six patients presented with Guillain-Barré syndrome that associated with COVID-19 infection, which was confirmed with RT-PCR. Here we discuss the laboratory investigation and case management, as well as clinical presentation and outcome of each case. The current report demonstrated the first case series of COVID-19-associated GBS-cases in Sudan.

Gene polymorphisms of cyclin-dependent kinase inhibitor and matrix metalloproteinase-9 in Sudanese patients with esophageal squamous cell carcinoma.

Background: The polymorphisms of the cyclin-dependent kinase inhibitor (CDKN1A) gene and matrix metalloproteinase-9 (MMP9) gene may increase one's susceptibility to malignancies. In this study, the association of the single nucleotide polymorphisms (SNPs) CDKN1A rs1059234 c.70C>T at the 3' untranslated region and MMP9 rs17576 (c.

The First Molecular Detection of in Sudan Associates with Increased Outbreaks of Chikungunya and Dengue.

As part of our surveys of the invasive malaria vector in four Sudanese states, including North and South Kordofan, Sennar, and White Nile, we collected 166 larvae. Our morphological identification confirmed that 30% of the collected mosquito samples were species, namely and , while the 117 specimens were (39%), (32%), (9%), (9%), (6%), (3%), and (3%). Considering the serious threat of emergence for the public health in the area and our limited resources, we prioritized samples for further genomic analysis.

Hepatitis E Virus Outbreak among Tigray War Refugees from Ethiopia, Sudan.

We report hepatitis E virus (HEV) outbreaks among refugees from Ethiopia in Sudan during June 2021-February 2022. We identified 1,589 cases of acute jaundice syndrome and used PCR to confirm HEV infection in 64% of cases. Implementing vaccination, water, sanitation, and hygiene programs might reduce HEV outbreak risk.

The use of cholera oral vaccine for containment of the 2019 disease outbreak in Sudan.

A cholera outbreak in Blue Nile and Sennar states, south-eastern and southern Sudan, took place during September-December 2019. An outbreak surveillance sample collection was made. Vibrio cholerae O1 Ogawa was isolated from clinical samples of all confirmed 200 and 132 cases in Blue Nile state and Sennar state, respectively.

Blood meal profile and positivity rate with malaria parasites among different malaria vectors in Sudan.

Background: Malaria is a life-threatening public health problem globally with particularly heavy burden in the sub-Saharan Africa including Sudan. The understanding of feeding preference of malaria vectors on different hosts is a major challenge for hindering the transmission cycle of malaria. In this study, blood meals taken by blood-fed Anopheles mosquitoes collected from the field in malaria endemic areas of Sudan were analysed for source of blood meal and malaria parasite presence.

The Phylodynamic and Spread of the Invasive Asian Malaria Vectors, , in Sudan.

is an invasive Asian malaria vector that initially emerged in Africa in 2012 and was reported in Sudan in 2019. We investigated the distribution and population structure of throughout Sudan by using sequencing and molecular tools. We confirmed the presence of in eight border-states, identifying both natural and human-made breeding sites.