Deciphering metabolic regulation in overripe grapes through multi-omics analysis of non-targeted metabolome, proteome, and transcriptome.

Background: As climate warms, the sugars and secondary metabolites in grapes gradually become uncoupled during development, resulting in the phenomenon of overripe fruit becoming more frequent around the world, especially in some arid regions. However, the key metabolic regulations to grape overripening are still poorly understood. To address this, we conducted a multi-omics study on Cabernet Sauvignon overripe berries over two years, analyzing the non-targeted metabolome, transcriptome, and proteome, aiming to provide a theoretical basis for delayed harvest strategies based on grape quality.

SGA-U-Net-Based Histopathological Assistive Diagnosis for Wilms Tumor Using Whole Slide Images.

Wilms tumor (WT) is the most prevalent renal malignancy in children. Determining its histopathological classification is critical for prognosis and postoperative treatment options. The histopathological classification of WT is based on the area percentages of its primary components, making accurate segmentation of these components essential for classification outcomes.

Afternoon shading delays ripening and modifies grape flavonoids and wine composition under natural heat stress in semi-arid regions.

To mitigate the rapid ripening by afternoon high temperature in semi-arid wine regions, grapevine artificial shading was applied in a three-year experiment (2021-2023) in China. The moderate shading (1.5 h, MD) and strong shading (2.

Domain Interactions in a Chimeric Dual-domain Lysin Lead to Broad Bactericidal Activity.

Phage-derived lysins represent a novel, non-traditional therapeutic agent against multidrug-resistant bacteria. However, engineering lysins with broad host range remain poorly addressed. Previously, we reported that the chimeric lysin ClyR, which harbors the CHAP catalytic domain of PlyC lysin (PlyCAC) and the SH3b cell-wall binding domain of PlySs2 lysin (PlySb), exhibits an expanded host range.

Research on quantitative prediction method of structural fractures in metamorphic rock reservoirs.

Deeply buried hill reservoirs have experienced tectonic movement during different periods, and tectonic fractures are well developed in metamorphic reservoirs, making quantitative characterization and spatial distribution prediction of fractures more difficult. Taking Block XX in the Bozhong Depression of the Bohai Bay Basin as an example, the development characteristics, formation periods and spatial distribution patterns of tectonic fractures in metamorphic buried hill reservoirs were clarified via core, thin section and image logging data. On the basis of 3D seismic and rock mechanics experimental data, geomechanical heterogeneity models of the study area in different periods were established, the 3D distributions of the tectonic stress field in different periods were clarified through finite element simulation, and a quantitative relationship between the tectonic stress and fracture linear density was established to quantitatively predict the spatial distribution characteristics of multistage tectonic fractures.

Flavoromics analysis and sensory evaluation of wines produced from nine red grape cultivars in Manas region, Xinjiang, China: Implications for terroir adaptation.

The synergistic interaction between grape cultivars and terroir fundamentally shapes wine quality and regional typicity. This three-year (2021-2023) study investigated the flavor profiles of wines produced from Cabernet Sauvignon, Cabernet Franc, Marselan, Merlot, Malbec, Syrah, Petit Verdot, Dornfelder, and Pinot Noir grown in Manas, Xinjiang, China, combining HPLC-MS, GC-MS, sensory evaluation, and multivariate analyses. Dornfelder, Marselan, and Petit Verdot exhibited better color due to their highest concentrations of monomeric anthocyanins, flavonols, and anthocyanin derivatives, respectively.

Machine learning-powered activatable NIR-II fluorescent nanosensor for in vivo monitoring of plant stress responses.

Real-time monitoring of plant stress signaling molecules is crucial for early disease diagnosis and prevention. However, existing methods are often invasive and lack sensitivity, rendering them inadequate for continuous monitoring of subtle plant stress responses. In this study, we develop a non-destructive near-infrared-II (NIR-II) fluorescent nanosensor for real-time detection of stress-related HO signaling in living plants.

Hsa_circ_0008085 acts as a miR-146a-5p sponge to suppress influenza a virus replication via modulating of TRAF6.

Influenza A virus (IAV) has attracted considerable attention in recent years due to its increase in incidence and threat to human health. Circular RNA (circRNA) is a non-coding RNA with a closed structural pattern, which often acts as a sponge for microRNAs (miRNAs) in the cell. A growing body of evidence supports several crucial roles for circRNAs in viral infection, including regulation of viral replication, evasion of the host immune response and disease pathogenesis.

Correlation between UGT1A1 polymorphism and efficacy and toxicity of irinotecan in Chinese cancer patients.

Objective: To assess the association between UGT1A1*6/*28 polymorphisms and Irinotecan (IRI) efficacy/toxicity in Chinese cancer patients.

Method: We systematically searched PubMed, Cochrane, CNKI, and Wanfang databases. Two investigators independently conducted literature screening, data extraction, and meta-analysis using Revman 5.

Whole-field mulching and removal indicated the flavonoid plasticity in Marselan grapes and wines: Insights from the metabolome and transcriptome.

As an eco-friendly vineyard floor management, mulching can effectively control weeds, preserve soil moisture, reduce irrigation requirements, and modify the grapevine microclimate. However, its impact on the grapevine microclimate, as well as the variation in grape and wine flavonoids across different growth stages under mulching conditions remains insufficiently studied. Therefore, in the vineyard of a semi-arid region, the whole-field mulching (M) of black geotextile mulch was applied to Vitis vinifera Marselan grapevines from anthesis and removed at three phenological stages.

Long noncoding RNA USP30-AS1 promotes influenza A virus replication by enhancing PHB1 function.

Long noncoding RNAs (lncRNAs) are important regulators of gene expression. Although evidence accumulated over the past decade shows that lncRNAs have key roles in the interaction between viruses and hosts, the functions of the majority of differentially expressed lncRNAs in response to viral infections remain uncharacterized so far. In this study, we have identified that USP30 antisense RNA 1 (USP30-AS1), a host antisense lncRNA, is hijacked by influenza A virus (IAV) to assist its replication.

Comprehensive analysis of publications concerning combinations of immunotherapy and targeted therapies for hepatocellular carcinoma: a bibliometric study.

Background: Hepatocellular carcinoma (HCC), a prevalent malignancy, is often diagnosed at advanced stages. Recent advances have integrated immunotherapy with targeted therapy, significantly improving treatment outcomes. This study provides a bibliometric overview of these therapeutic combinations, evaluating their development and impact.

Anti-reflux mucosectomy for proton pump inhibitor refractory gastroesophageal reflux disease with hiatal hernia: A case report.

Introduction: Gastroesophageal reflux disease (GERD) is a prevalent chronic condition primarily treated with proton pump inhibitors (PPIs). However, patients with PPI-refractory GERD and hiatal hernia present significant therapeutic challenges, with conventional treatments often yielding unsatisfactory outcomes. While anti-reflux mucosectomy (ARMS) has emerged as a promising treatment, its efficacy in cases with hiatal hernia remains understudied.

Application value of F-FDG PET/CT in soft tissue metastasis of intrahepatic cholangiocarcinoma: a case report and literature review.

Intrahepatic cholangiocarcinoma (ICC)originates from the epithelial cells of the intrahepatic bile ducts, with insidious onset and strong invasiveness, and most of the cases are found in the advanced stage, with extremely poor prognosis. In advanced stages, distant metastases to the lungs, bones, and brain are common, but distant soft tissue (subcutaneous and skeletal muscle) and breast metastases are rare, and simultaneous metastases to all three rare sites had not been reported. We report a 69-year-old woman with right upper abdominal pain who underwent a plain and enhanced CT scan of the upper abdomen, which revealed an intrahepatic space-occupying lesion, as well as subcutaneous and peritoneal nodules in the abdomen.

N -Acetyl-Tryptophan in Acute Kidney Injury after Cardiac Surgery.

Key Points: -acetyl-tryptophan (NAT) was elevated in plasma of patients with cardiac surgery–associated AKI compared with those who do not develop AKI after surgery. NAT exhibited kidney-protective effects in ischemia-reperfusion–, cisplatin-, and unilateral ureteral obstruction–induced kidney injury mouse models and epithelial cellular models. NAT interacted with Kelch-like ECH-associated protein 1 at 483 and 508 sites, resulting in Nrf2 nuclear translocation and proteasome genes transcription, respectively.

Detection of TNF-α using the established ab-MPs-CLIA.

Tumor necrosis factor alpha (TNF-α) is a key cytokine in inflammation and immune responses, making its rapid and accurate detection essential for disease diagnosis and management. In this study, we developed a highly sensitive chemiluminescence immunoassay (CLIA) using antibody-coated magnetic particles (Ab-MPs-CLIA) for TNF-α detection. From nine candidate antibodies, we identified an optimal pair through epitope competition and affinity assessments, significantly improving assay performance.

Broad-Spectrum Engineered Multivalent Nanobodies Against SARS-CoV-1/2.

SARS-CoV-2 Omicron sublineages escape most preclinical/clinical neutralizing antibodies in development, suggesting that previously employed antibody screening strategies are not well suited to counteract the rapid mutation of SARS-CoV-2. Therefore, there is an urgent need to screen better broad-spectrum neutralizing antibody. In this study, a comprehensive approach to design broad-spectrum inhibitors against both SARS-CoV-1 and SARS-CoV-2 by leveraging the structural diversity of nanobodies is proposed.

A novel method of cardia visualization and comfort level enhancement during magnetic capsule gastroscopy via sugar-glued tether-assisted technique: a randomized pilot study inspired by a Chinese snack-making process (with video).

Background: Magnetic capsule gastroscopy (MCG) is a non-invasive diagnostic method for the digestive tract. However, its efficiency in visualizing the gastric cardia is often compromised due to the capsule's rapid passage. This study introduces a novel sugar-glued tether-assisted technique inspired by a traditional Chinese snack-making process to enhance cardia visualization and patient comfort during MCG.

LINC01197 inhibits influenza A virus replication by serving as a PABPC1 decoy.

Influenza A viruses (IAVs) significantly impact animal and human health due to their zoonotic potential. A growing body of evidence indicates that the host's long noncoding RNAs (lncRNAs) play crucial roles in regulating host-virus interactions during IAV infection. However, numerous lncRNAs associated with IAV infection have not been well characterised.

CD133/ABCC5 cervical cancer cells exhibit cancer stem cell properties.

Objective: This study explores the correlation between Forkhead box M1 (FOXM1) and ATP-binding cassette subfamily C member 5 (ABCC5) in relation to paclitaxel resistance in cervical cancer. It aims to identify potential cervical cancer stem cell markers, offering fresh perspectives for developing therapeutic strategies to overcome paclitaxel chemoresistance in cervical cancer.

Methods: Paclitaxel-resistant Hela cells (Hela/Taxol) were developed by intermittently exposing Hela cells to progressively increasing concentrations of paclitaxel.