A Biotechnological Approach to Insect Waste Valorization: Silkworm Pupae as a Functional Feed Ingredient for Pangasius pangasius, Improving Growth and Physiological Responses.

The rising cost of fish meal and soybean meal for aquafeeds has encouraged researchers to look for alternative protein sources. To find an alternative, this study was designed to replace soybean meal with silkworm pupae meal. An experimental feeding trial of 75 days was conducted on Pangasius fingerlings to assess the effects of partially substituted soybean meal with silkworm pupae meal (SWPM) on growth performance, antioxidant activity, and digestive enzymes.

Hematological, Histological, and NMR Analysis of Meat Quality and Growth Performance of Broilers Fed with Probiotics Bacillus licheniformis-Supplemented Poultry Feed.

The present study evaluated the effect of probiotics (Bacillus licheniformis) on the growth pattern, hematological parameters, metabolic profile, and meat quality of broiler birds. Total of 75 Ross 308 broiler chicks were divided into three groups (25 broilers in each) and fed with poultry feed supplemented with probiotics (15 g/ton of feed for starter diet and 35 g/ton for grower diet), antibiotic (amoxicillin 900 mg/ton of feed) and basal poultry feed, respectively, for 5 weeks. The broilers of control group exhibited higher feed conversion rate (FCR), i.

Covalent immobilization of thermotolerant recombinant nano-coupled xylanase for improved stability and reusability in the saccharification process.

Xylanase, a key enzyme in the saccharification of lignocellulosic biomass (LCB) for bioethanol production, often faces limitations due to its limited reusability and poor stability. For this purpose, β-1,4-xylanase gene (Clocl_0045) of 1239 bp from Clostridium clariflavum (also known as Acetivibrio clariflavus) was cloned and expressed into expression system i.e.

Anti-biofilm potential of some fish probiotics, alone and in combination with antibiotics against isolated aquaculture pathogens; A preliminary data.

This study aims to isolate and identify both diseased and healthy fish pathogens of Ctenopharyngodon idella, Labeo rohita and Oreochromis niloticus and assess their antibacterial and biofilm supressing activities against fish pathogens. It explores their potential to inhibit and degrade biofilms, serving as an alternative to antibiotics in aquaculture while enhancing fish health and disease resistance. Furthermore, the research endeavors to assess the biofilm degradation potential of antibiotics and probiotics, both individually and in combination.

Medicinal Plants Exhibited Promising Potential to Inhibit Biofilm Formation by Catheter-Associated Bacteria in UTI Patients from Lahore, Pakistan.

The current study was designed to evaluate the antibacterial, antibiofilm, and biofilm inhibitory potential of six medicinal plants, including Trachyspermum ammi, Trigonella foenum-graecum, Nigella sativa, Thymus vulgaris, Terminalia arjuna, and Ipomoea carneaid against catheter-associated bacteria (CAB). Eighteen CAB were identified up to species level using 16S rRNA gene sequencing, viz., Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa.

Antibiofilm and Antiquorum Sensing Potential of Pheretima posthum.

Antibiotic resistance is a world wide problem mainly in developing countries. In this work, coelomic fluid (PCF) and paste (PBP) of Pheretima posthuma was assessed for its potential as antibiofilm and anti-quorum sensing (QS) agent against pathogenic bacterial biofilms. PCF and PBP were extracted and biofilm formation time kinetics was examined using crystal violet staining method by utilizing four bacterial isolates in bispecies biofilm (06 combinations; MH5-MH10) and multi species biofilms (05 combinations; MH11-MH15).

Effective xylan integration for remodeling biochar uniformity and porosity to enhance chemical elimination and CO adsorption.

Although plant evolution has offered diverse biomass resources, the production of high-quality biochar from desirable lignocelluloses remains unexplored. In this study, distinct lignocellulose substrates derived from eight representative plant species were employed to prepare biochar samples under three different temperature treatments. Correlation analysis showed that only hemicellulose was a consistently positive factor of lignocellulose substrates to account for the dye-adsorption capacities of diverse biochar samples.

Laccase Production Optimization from Recombinant BL21 Codon Plus Containing Novel Laccase Gene from for Removal of Wastewater Textile Dye.

The aim of the present research was the efficient degradation of industrial textile wastewater dyes using a very active cloned laccase enzyme. For this purpose, potent laccase-producing bacteria were isolated from soil samples collected from wastewater-replenished textile sites in Punjab, Pakistan. The laccase gene from locally isolated strain LI-81, identified as , was cloned into vector pET21a, which was further transformed into BL21 codon plus.

The Characterization and Study of Antibacterial, Free Radical Scavenging, and Anticancer Potential of -Mediated Silver Nanoparticles.

In the present research, leaf extracts were utilized as reductants to bio-fabricate silver nanoparticles (LC-AgNPs) and this was followed by the evaluation of their antioxidant, antibacterial, and anticancer potential. Multiple parameters were optimized for the formation and fidelity of LC-AgNPs. The color shift of the reaction mixture from yellow to dark brown confirmed the LC-AgNPs formation.

In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms.

Overuse of pesticides in agricultural soil and dye-polluted effluents severely contaminates the environment and is toxic to animals and humans making their removal from the environment essential. The present study aimed to assess the biodegradation of pesticides (cypermethrin (CYP) and imidacloprid (IMI)), and dyes (malachite green (MG) and Congo red (CR)) using biofilms of bacteria isolated from pesticide-contaminated soil and dye effluents. Biofilms of indigenous bacteria, i.

BsEXLX of engineered Trichoderma reesei strain as dual-active expansin to boost cellulases secretion for synergistic enhancement of biomass enzymatic saccharification in corn and Miscanthus straws.

In this study, bacterial BsEXLE1 gene was overexpressed into T. reesei (Rut-C30) to generate a desirable engineered TrEXLX10 strain. While incubated with alkali-pretreated Miscanthus straw as carbon source, the TrEXLX10 secreted the β-glucosidases, cellobiohydrolases and xylanses with activities raised by 34%, 82% and 159% compared to the Rut-C30.

Bioremediation of heavy metals polluted environment and decolourization of black liquor using microbial biofilms.

Background: With increased urbanization and industrialization, modern life has led to an anthropogenic impact on the biosphere. Heavy metals pollution and pollutants from black liquor (BL) have caused severe effects on environment and living organisms. Bacterial biofilm has potential to remediate heavy metals and remove BL from the environment.

Antibiofilm Potential of Coelomic Fluid and Paste of Earthworm (Clitellata, Megascolecidae) against Pathogenic Bacteria.

Antibiotic drug resistance is a global public health issue that demands new and novel therapeutic molecules. To develop new agents, animal secretions or products are used as an alternative agent to overcome this problem. In this study, earthworm () coelomic fluid (PCF), and body paste (PBP) were used to analyze their effects as antibiofilm agents against four bacterial isolates MH1 ( MT448672), MH2 ( MT448673), MH3 ( MT448675), and MH4 ( MT448676).

Enzymatic hydrolysis of low temperature alkali pretreated wheat straw using immobilized β-xylanase nanoparticles.

A low temperature alkali (LTA) pretreatment method was used to treat wheat straw. In order to obtain good results, different factors like temperature, incubation time, NaOH concentration and solid to liquid ratio for the pretreatment process were optimized. Wheat straw is a potential biomass for the production of monomeric sugars.

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene from for detergent industry.

Present study was aimed to clone and express the esterase encoding gene from in BL21. Purification of recombinant esterase enzyme was achieved up to 48.6 purification folds by ion exchange chromatography with specific activity of 126.

Intermittent ultrasound retains cellulases unlock for enhanced cellulosic ethanol with high-porosity biochar for dye adsorption using desirable rice mutant straw.

In this study, optimal ultrasound pretreatment was performed with recalcitrance-reduced rice mutant straw to effectively extract lignin and hemicellulose for improved cellulose accessibility. Intermittent ultrasound-assistant enzymatic hydrolyses were followed to maintain more cellulases unlock and less cellulose surface block with lignin for raised hexose yield at 81 % (% cellulose) and bioethanol concentration at 9.9 g/L, which was higher than those of other mechanical pretreatments as previously conducted.

Current advances in the classification, production, properties and applications of microbial biosurfactants - A critical review.

This review discusses the classification, characteristics, and applications of biosurfactants. The biosynthesis pathways for different classes of biosurfactants are reviewed. An in-depth analysis of reported research is carried out emphasizing the synthetic pathways, culture media compositions, and influencing factors on production yield of biosurfactants.

Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from : a potential addition for biofuel industry.

The present study describes the cloning, expression, purification and characterization of the xylosidase gene (1650 bp) from a thermophilic bacterium into BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production. The recombinant xylosidase enzyme was purified to homogeneity by heat treatment and immobilized metal ion affinity chromatography. SDS-PAGE determined that the molecular weight of purified xylosidase was 60 kDa.

Correction: Efficient biomass saccharification using a novel cellobiohydrolase from for utilization in biofuel industry.

[This corrects the article DOI: 10.1039/D1RA00545F.].

Effective utilization of magnetic nano-coupled cloned β-xylanase in saccharification process.

The β-xylanase gene (DCE06_04615) with 1041 bp cloned from was expressed into BL21 DE3. The cloned β-xylanase was covalently bound to iron oxide magnetic nanoparticles coated with silica utilizing carbodiimide. The size of the immobilized MNPs (50 nm) and their binding with β-xylanase were characterized by Fourier-transform electron microscopy (FTIR) (a change in shift particularly from C-O to C-N) and transmission electron microscopy (TEM) (spherical in shape and 50 nm in diameter).

Efficient biomass saccharification using a novel cellobiohydrolase from for utilization in biofuel industry.

The present study describes the cloning of the cellobiohydrolase gene from a thermophilic bacterium and its expression in BL21(DE3) utilizing the expression vector pET-21a(+). The optimization of various parameters (pH, temperature, isopropyl β-d-1-thiogalactopyranoside (IPTG) concentration, time of induction) was carried out to obtain the maximum enzyme activity (2.78 ± 0.

Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice.

Background: Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite genetic modification of major wall polymers has been implemented for reduced recalcitrance in engineered crops, it could most cause a penalty of plant growth and biomass yield. Alternatively, it is increasingly considered to improve minor wall components, but an applicable approach is required for efficient assay of large population of biomass samples.