Dynamics of urinary tract infections: a comprehensive study on antimicrobial susceptibility, virulence profiling and molecular epidemiology of uropathogenic Escherichia coli from Pakistan.

Background: Urinary tract infections (UTIs), primarily caused by Escherichia coli, pose a global health challenge owing to rising antimicrobial resistance (AMR), particularly carbapenem-resistant (Carb-RT) and extended-spectrum beta-lactamase (ESBL)-producing uropathogenic E. coli (UPEC). This study analyzed AMR trends, virulence determinants, and molecular epidemiology of UPEC isolates from community and hospital settings in Pakistan.

Antimicrobial resistance and virulence profiling of Klebsiella pneumoniae isolated from urinary tract infections in community and hospital settings in Pakistan.

Urinary tract infections (UTIs) caused by multidrug-resistant (MDR) bacteria pose escalating challenges in resource-limited settings. This cross-sectional study addresses critical gaps in molecular surveillance by directly comparing antimicrobial resistance (AMR) genotypes, virulence factors, and resistance phenotypes of K. pneumoniae isolates from community (CA-UTI) and hospital-acquired (HA) UTI.

Carbapenem Resistant Serratia marcescens Harboring bla, armA and aac(6')-Ib-cr Genes: The First Baseline Study from Pakistan.

The emergence of Serratia marcescens as a significant healthcare-associated pathogen is compounded by its rising carbapenem resistance resulting in a critical global health challenge. This study is the first in Pakistan to comprehensively characterize the antimicrobial resistance patterns and molecular mechanisms of S. marcescens from clinical specimens.

Bioinspired synthesis and characterization of chitosan-based MgO nanoparticles: Evaluating antibacterial potential against MDR pathogens and anticancer activity in HEPG2 cell lines.

The growing antibacterial resistance threatens the public health and medical sector. The concern necessitates the innovative and environmentally friendly solution which is addressed in the current study by synthesis of eco-friendly biopolymer-based metallic oxide nanoparticles using green synthesis, offering a sustainable alternative to conventional approaches. Chitosan-based magnesium oxide (CH-MgO) nanoparticles were synthesized using leaf extract of Trianthema portulacastrum.

Carbapenem-Resistant Complex Isolates Carrying and in Ventilator-Associated Pneumonia Patients and Contaminated Ventilator Tubing.

Ventilator-associated pneumonia (VAP) represents an important nosocomial infection, frequently encountered in intensive care unit (ICU) settings which results in prolonged hospitals stays. The nosocomial infections caused by complex (BCC) bacteria pose a significant challenge in healthcare settings owing to their intrinsic resistance to many antibiotics. This study investigates the antimicrobial susceptibility patterns and mechanisms of carbapenem resistance among BCC bacteria from VAP patients and the ventilator tubing.

Distribution of multidrug-resistant in poultry, livestock, fish, and the related environment: One Health heed.

Background And Aim: The emergence of multidrug-resistant (MDR) in food-producing animals and their associated environments is a growing public health concern. The indiscriminate use of antimicrobials in animal husbandry exacerbates resistance development, posing significant threats to food safety and sustainability. This study investigates the distribution, antibiotic resistance patterns, and virulence-associated genes (VAGs) of isolated from poultry, livestock, fish, and their environments in Pakistan under a One Health perspective.

Lumpy Skin Disease: Insights into Molecular Pathogenesis and Control Strategies.

Lumpy skin disease (LSD) is a viral infection that affects buffaloes and cattle across various regions, including both tropical and temperate climates. Intriguingly, the virus-carrying skin sores remain the primary source of infection for extended periods, exacerbated by the abundance of vectors in disease-endemic countries. Recent scientific advances have revealed the molecular aspects of LSD and offered improved vaccines and valuable antiviral targets.

Characterizing the bacteriophage PKp-V1 as a potential treatment for ESBL-producing hypervirulent K1 ST258 isolated from veterinary specimens.

Background And Aim: The dearth of new antibiotics necessitates alternative approaches for managing infections caused by resistant superbugs. This study aimed to evaluate the lytic potential of the purified bacteriophage PKp-V1 against extended-spectrum β-lactamase (ESBL) harboring hypervirulent (hvKp)-K1 recovered from veterinary specimens.

Materials And Methods: A total of 50 samples were collected from various veterinary specimens to isolate , followed by antimicrobial susceptibility testing and molecular detection of various virulence and ESBL genes.

Potential Target Metabolites From Gut Microbiota Against Hepatocellular Carcinoma: A Network Pharmacology and Molecular Docking Study.

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide, posing significant challenges and economic burdens on healthcare systems. Gut microbiota metabolites have shown promise in cancer treatment, but the specific active metabolites and their key targets remain unclear. This study employed a network pharmacology-based approach to identify potent metabolites of gut microbiota and their key targets.

AMR and Sustainable Development Goals: at a crossroads.

Antimicrobial resistance (AMR) poses a significant global health threat, primarily stemming from its misuse and overuse in both veterinary and public healthcare systems. The consequences of AMR are severe, leading to more severe infections, increased health protection costs, prolonged hospital stays, unresponsive treatments, and elevated fatality rates. The impact of AMR is direct and far-reaching, particularly affecting the Sustainable Development Goals (SDGs), underscoring the urgency for concerted global actions to achieve these objectives.

Predominance of - and -Harboring Belonging to Clonal Complexes 131 and 23 in a Major University Hospital.

: Carbapenem resistance is a growing global challenge for healthcare, and, therefore, monitoring its prevalence and patterns is crucial for implementing targeted interventions to mitigate its impact on patient outcomes and public health. This study aimed to determine the prevalence of carbapenem resistance among () strains in the largest tertiary care hospital of the capital territory of Pakistan and to characterize the isolates for the presence of antimicrobial resistance genes. Additionally, the most prevalent sequence types were analyzed.

From promise to progress: the dynamic landscape of glioblastoma immunotherapy.

Glioblastoma multiforme (GBM) is the most common CNS cancer, it has dismal survival rates despite several effective mediators: intensified cytotoxic therapy, chimeric antigen receptor (CAR)-T cell therapy, viral therapy, adoptive cell therapy, immune checkpoint blockade therapy, radiation therapy and vaccine therapy. This review examines the basic concepts underlying immune targeting and examines products such as checkpoint blockade drugs, CAR-T cells, oncolytic viruses, combinatory multimodal immunotherapy and cancer vaccines. New approaches to overcoming current constraints and challenges in GBM therapy are discussed, based on recent studies into these tactics, findings from ongoing clinical trials, as well as previous trial results.

Uncovering chikungunya virus-encoded miRNAs and host-specific targeted genes associated with antiviral immune responses: an integrated bioinformatics approach.

Chikungunya virus (CHIKV) is a single-stranded RNA virus belonging to the genus Alphavirus and is responsible for causing Chikungunya fever, a type of arboviral fever. Despite extensive research, the pathogenic mechanism of CHIKV within host cells remains unclear. In this study, an in-silico approach was used to predict that CHIKV produces micro-RNAs that target host-specific genes associated with host cellular regulatory pathways.

Breaking barriers in cancer treatment: nanobiohybrids empowered by modified bacteria and vesicles.

Cancer, the leading global cause of mortality, poses a formidable challenge for treatment. The effectiveness of cancer therapies, ranging from chemotherapy to immunotherapy, relies on the precise delivery of therapeutic agents to tumor tissues. Nanobiohybrids, resulting from the fusion of bacteria with nanomaterials, constitute a promising delivery system.

Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence.

Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo hemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi virus (KSIV) based on a newly established luciferase immunoprecipitation system (LIPS) assays, and Zika virus (ZIKV) by enzyme-linked immunosorbent assays (ELISA).

The Unspoken Wounds: Understanding the Psychological Impact on Healthcare Professionals Fighting COVID-19 in Pakistan.

During the COVID-19 pandemic, hospital staff faced numerous mental health challenges. However, limited research focused on anxiety and stress specifically among hospital workers during this time. Therefore, this study aimed to investigate the anxiety levels of healthcare workers during the COVID-19 pandemic.

Deciphering Multi-target Pharmacological Mechanism of Seeds against Kidney Stones: Network Pharmacology and Molecular Docking Approach.

Background: Urolithiasis is a prevalent condition with significant morbidity and economic implications. The economic burden associated with urolithiasis primarily stems from medical expenses. Previous literature suggests that herbal plants, including , have lithotriptic capabilities.

From Pixels to Pathology: Employing Computer Vision to Decode Chest Diseases in Medical Images.

Radiology has been a pioneer in the healthcare industry's digital transformation, incorporating digital imaging systems like picture archiving and communication system (PACS) and teleradiology over the past thirty years. This shift has reshaped radiology services, positioning the field at a crucial junction for potential evolution into an integrated diagnostic service through artificial intelligence and machine learning. These technologies offer advanced tools for radiology's transformation.

Pancreatic regenerative potential of manuka honey evidenced through pancreatic histology and levels of transcription factors in diabetic rat model.

Background: Diabetes mellitus is a commonly occurring metabolic disorder accompanied by high morbidity and alarming mortality. Besides various available therapies, induction of pancreatic regeneration has emerged as a promising strategy for alleviating the damaging effect of diabetes. Honey, a potent antioxidative and anti-inflammatory agent, has been reported in the literature archive to exhibit favourable results in the regeneration process of several organ systems.

Exploring the recent developments of alginate silk fibroin material for hydrogel wound dressing: A review.

Hydrogels, a type of polymeric material capable of retaining water within a three-dimensional network, have demonstrated their potential in wound healing, surpassing traditional wound dressings. These hydrogels possess remarkable mechanical, chemical, and biological properties, making them suitable scaffolds for tissue regeneration. This article aims to emphasize the advantages of alginate, silk fibroin, and hydrogel-based wound dressings, specifically highlighting their crucial functions that accelerate the healing process of skin wounds.

Comparative analysis of phyto-fabricated chitosan, copper oxide, and chitosan-based CuO nanoparticles: antibacterial potential against isolates and anticancer activity against HepG2 cell lines.

The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of . The synthesized nanoparticles were characterized using different techniques, such as the synthesis of the particles, which was confirmed by UV-visible spectrometry that showed absorbance at 300 nm, 255 nm, and 275 nm for the CH, CuO, and CH-CuO nanoparticles, respectively.