An Olive Oil-Based High-Fat Diet Promotes Obesity-Driven Metastasis of Triple-Negative Breast Cancer.

Obesity is strongly associated with triple-negative breast cancer (TNBC). A better understanding of the molecular mechanisms driving obesity-induced TNBC progression could facilitate development of precision dietary intervention strategies. Here, we used murine models of obesity induced by different high-fat diets (HFDs) to examine their impact on TNBC progression.

Perioperative outcomes of the quadriceps-sparing subvastus approach versus medial parapatellar approach for primary total knee arthroplasty.

Aims & Objectives: The quadriceps-sparing subvastus (SV) approach for total knee arthroplasty (TKA) is theorized to prevent extensor mechanism injury, accelerate recovery and is often compared to the medial parapatellar (MPP) approach. We aimed to compare postoperative outcomes of SV and MPP approaches after TKA in the veteran population.

Materials & Methods: We conducted a retrospective cohort study of all unilateral primary TKAs performed at our institution with an MPP or SV approach between January 1st, 2017 and December 31st, 2020.

Multirod Constructs in Spine Surgery.

➢ The use of multirod constructs (≥3 rods) in complex spine surgery has increased as its utility has been recognized over the past decade.➢ There are multiple different rod configurations that may be utilized on the basis of the desired supplemental rod function, with each type having its own advantages and clinical indications.➢ Literature has continued to demonstrate a reduced incidence of pseudarthrosis, rod fracture, and reoperation when comparing multirod constructs with traditional dual-rod constructs.

Open Reduction and Internal Fixation of a Volar Displaced Salter-Harris III Mallet Fracture in a Pediatric Patient: A Case Report.

Finger injuries are common in pediatric patients and typically heal well with conservative management. However, rare fracture patterns involving significant displacement and physeal injury, such as the one described in this case, require specialized surgical intervention to ensure proper healing and prevent long-term complications. A 12-year-old left-hand-dominant female presented with pain, swelling, and deformity at the distal interphalangeal (DIP) joint following hyperextension of the left fifth digit.

Emergency Department Presentations for Low Back Pain by Remoteness and Socioeconomic Status in New South Wales: A Population-Based Study.

Objective: To explore the patterns of emergency department (ED) presentations for low back pain (LBP) by remoteness and socioeconomic status (SES) in New South Wales (NSW).

Methods: A retrospective, population-based study of deidentified data was undertaken with data sourced from the NSW Emergency Department Data Collection (EDDC). The study population comprised NSW residents who presented to an NSW public hospital ED in 2013-2019 for LBP and were registered in the NSW EDDC.

RNA Pol II inhibition activates cell death independently from the loss of transcription.

RNA Pol II-mediated transcription is essential for eukaryotic life. Although loss of transcription is thought to be universally lethal, the associated mechanisms promoting cell death are not yet known. Here, we show that death following the loss of RNA Pol II activity does not result from dysregulated gene expression.

Respiratory Failure in Patients with Intracerebral Hemorrhage and Intraventricular Hemorrhage Extension: A Retrospective Study.

This study aimed to identify the risk factors for respiratory failure in patients with intracerebral hemorrhage (ICH) accompanied by intraventricular hemorrhage (IVH) extension. : We retrospectively included 208 patients with ICH accompanied by IVH extension. Respiratory failure was defined as carbon dioxide levels > 45 mmHg with a pH < 7.

Protein kinase CK2α' as a dual modulator of neuroimmune signaling and synaptic dysfunction in Tauopathy.

Background: Tauopathies are a group of neurodegenerative diseases characterized by tau accumulation, neuroinflammation, and synaptic dysfunction, yet effective treatments remain elusive. Protein Kinase CK2 has been previously associated with different aspects of tau pathology but genetic evidence for the contribution of CK2 to tauopathy remained lacking.

Methods: We used cell and mouse models to explore the impact of CK2α' in tauopathy.

PgtE protease enables virulent to evade C3-mediated serum and neutrophil killing.

Non-typhoidal serovars, such as serovar Typhimurium (STm), are a leading cause of inflammatory diarrhea in otherwise healthy individuals. Among children, the elderly, and immunocompromised individuals, STm can spread to systemic sites and cause potentially lethal bacteremia. Phagocytic cells and the immune complement system are pivotal to preventing the dissemination of STm.

Heterochromatin-based silencing of a foreign tandem repeat in shows unusual biochemistry and temperature sensitivity.

Eukaryotic genomes are packaged into chromatin, a regulatory nucleoprotein assembly. Establishment, maintenance, and interconversion of chromatin states is required for correct patterns of gene expression, genome integrity, and organismal survival. Transcriptionally repressive heterochromatin minimizes mobilization of transposable elements and limits expansion of other repetitive DNA, but mechanisms for recognition of the latter sequences are not well established.

Engineering Chinese Hamster Ovary Cells to Mitigate Polysorbate Degradation in Biotherapeutics.

A recurring root cause for polysorbate degradation and associated particle observations in liquid biotherapeutics is the presence of residual host cell proteins (HCPs). Some of these HCPs can hydrolyze the polysorbate surfactant to release poorly-soluble fatty acids that accumulate over time and form particles. To tackle this industry-wide issue, we disrupted the genes for a plurality of potential polysorbate-degrading HCPs in CHO cells via two CHO cell engineering approaches.

Worldwide procedural variations of primary percutaneous coronary intervention for ST-elevation myocardial infarction: Insights from the "Stent - Save a Life!" initiative.

Background: ST-elevation myocardial infarction (STEMI) outcomes have improved through initiatives that promote timely access to primary percutaneous coronary intervention (PCI). However, little is known about how primary PCI is performed across different settings. This study proposes to characterize and compare practical aspects of primary PCI globally.

Pericardial Conundrum: Unmasking Tuberculosis as the Culprit.

Background: Tuberculosis, caused by Mycobacterium tuberculosis, primarily affects the lungs but can involve other organs, termed extrapulmonary tuberculosis. Tuberculous pericarditis (TBP) is a rare form, representing approximately 1% of tuberculosis-related autopsies and 4% of acute pericarditis cases in developed countries.

Case Summary: A 29-year-old healthy Indian man presented with fever, night sweats, and weight loss.

A comparison of deep versus awake tracheal extubation in adults: a randomized controlled trial.

Background: Awake extubation is deemed a generally safe option for tracheal extubation in low-risk patients, while anesthetized, or"deep" extubation is often considered best suited to seasoned providers due to its perceived hazards. However, inadequate data exists about the relative safety of deep and awake extubations in the adult population.

Methods: Adult patients (n=220) with favorable airways undergoing elective surgeries under general tracheal anesthesia were enrolled prospectively.

Oral Prodrug of a Novel Glutathione Surrogate Reverses Metabolic Dysregulation and Attenuates Neurodegenerative Process in Transgenic Alzheimer's Mice.

Glycation-induced oxidative stress underlies the numerous metabolic ravages of Alzheimer's disease (AD). Reduced glutathione levels in AD lead to increased oxidative stress, including glycation-induced pathology. Previously, we showed that the accumulation of reactive 1,2-dicarbonyls such as methylglyoxal, the major precursor of nonenzymatic glycation products, was reduced by the increased function of GSH-dependent glyoxalase-1 enzyme in the brain.

Pattern of change in force and moment according to amounts of labial tipping and material types of clear aligner: experiment with a six-axis mechanical sensor apparatus.

Objective: To investigate the pattern of change in force and moment according to the amounts of labial tipping and the material types of clear aligners (CA).

Methods: A 3D-printed maxillary typodont system, including crown, root, and periodontal ligament space, was constructed. Six groups were established based on the amount of labial tipping of #12 (0.

Identification of CT features and laboratory indicators to aid in the management of patients with portal venous gas identified on imaging.

Background: It can be difficult to distinguish cases of benign portal venous gas (PVG) that can be managed conservatively from forms associated with ischaemic bowel.

Purpose: To identify CT, clinical and laboratory factors that may be assist in the decision-making regarding patient management.

Methods: All patients in whom PVG was demonstrated on CT imaging were identified over an 8-year period.

Forces and moments on a maxillary lateral incisor using 3D-printed aligners with pressure points: an in vitro study.

Purpose: This study aimed to compare the initial forces and moments exerted by 3D-printed clear aligners on a maxillary left lateral incisor during distal rotation using pressure points and/or relief channels.

Materials And Methods: An in vitro setup using multi-axis force/moment transducers measured forces and moments on tooth 22, rotated 3° distally. Six experimental groups were tested, using TC-85 clear resin aligners with varying pressure points (PP) and relief channel configurations: Group 1 (CON): Control with no PPs or channels; Group 2 (FP): Facial PP on the distofacial aspect; Group 3 (LP): Lingual PP on the mesiolingual aspect; Group 4 (DBL): Combined facial and lingual PPs; Group 5 (FWR): FP with a distolingual relief channel; and Group 6 (LWR): LP with a mesiofacial relief channel.

Establishment of Initiated Cell Line Derived From NIH3T3 Fibroblasts.

Autophagy plays contrasting roles depending on the stage of cellular transformation. However, although advanced tumor cell models are abundant, cell lines at the initiation stage of transformation are very limited. Therefore, the development of initiated cell lines-cells that have acquired early genetic alterations but not yet completed the multistep transformation process -is crucial for the development of anticancer drugs targeting autophagy.

Spina bifida as a multifactorial birth defect: Risk factors and genetic underpinnings.

Spina bifida is a birth defect resulting from abnormal embryonic development of the neural tube. Though spina bifida is divided into several subtypes, myelomeningocele-the most severe form of spina bifida often associated with a markedly diminished quality of life-accounts for a significant portion of cases. A broad range of genetic and environmental factors, many of which are still unknown, influence spina bifida, making it difficult to provide a comprehensive etiology for the disorder.