Fate of Salmonella, Escherichia coli, and Listeria monocytogenes in dark leafy green vegetable juice at different storage temperatures.

Dark leafy green vegetables (DLGVs), including kale, chard, and collard greens, have gained increasing attention for their rich nutrient profiles and health benefits, driving the development of derivative products such as dark leafy green vegetable juices (DLGVJs). However, fresh DLGVJs are susceptible to contamination by foodborne pathogens due to inadequate hygiene, improper handling, and cross-contamination. Additionally, temperature abuse during storage and distribution may further compromise their safety.

Associated factors and mediating effects on traditional Chinese medicine health information literacy in undergraduate nursing students.

Objective: To investigate the current status and associated factors of health information literacy in Traditional Chinese Medicine (TCM) among undergraduate nursing students and explore the mediating effects of these factors, with the aim of providing empirical evidence for optimizing the reform plan of TCM nursing curriculum.

Methods: From November 2023 to December 2024, 215 undergraduate nursing students were selected as the research subjects and investigated using a general information questionnaire, the Health information literacy scale in TCM, and the scale of autonomous learning competencies of nursing undergraduates.

Results: The overall scores of health information literacy in TCM of undergraduate nursing students was (122.

Resistome and microbiome shifts in catfish rearing water: the influence of temperature and antibiotic treatments.

The increasing reliance on aquaculture for sustainable protein production highlights the need for responsible antibiotic use to manage bacterial infections, particularly in intensive farming systems. This study investigated the effects of three FDA-approved antibiotics (Aquaflor®, Romet®, Terramycin®) at common fish bacterial disease outbreak temperatures (20 °C, 25 °C, and 30 °C) on the microbiome and resistome of aquaculture water using a catfish model system. Metagenomic analyses evaluated the abundance, diversity, and mobility of antimicrobial resistance genes (ARGs) and antibiotic-resistant bacteria (ARB).

The impact of florfenicol treatment on the microbial populations present in the gill, intestine, and skin of channel catfish (Ictalurus punctatus).

Background: Florfenicol is a broad-spectrum antimicrobial approved in many countries for treating bacterial infections in production animals. Although florfenicol has been widely used in the US catfish industry, its impact on the native microbiota within catfish tissues remains largely unknown. Florfenicol treatment is followed by a mandatory withdrawal period to ensure drug residues fall below regulatory limits before harvest.

Cross-cultural adaptation and validation of the Chinese version of the Malnutrition Awareness Scale among community-dwelling older adults.

Objective: To translate, cross-culturally adapt, and validate the Malnutrition Awareness Scale (MAS) among Chinese community-dwelling older adults.

Methods: This study consists of two parts. First, we developed the Chinese version of the MAS (C-MAS) through direct translation, back translation, cross-cultural adaptation, and pilot testing.

Establishment of CRISPR-STAR System to Realise Simultaneous Transcriptional Activation and Repression in Yarrowia lipolytica.

The ability to regulate gene expression in multiple directions is crucial to maximise the production of microbial cell factories. However, the lack of a regulatory tool that can simultaneously activate and repress multiple genes restricts the manipulation diversity of Yarrowia lipolytica, which is an industrial workhorse for bioproduction. To address this issue, we developed a CRISPR scaffold RNAs (scRNAs)-mediated transcriptional activation and repression (CRISPR-STAR) platform.

Effects of resistance training on breast cancer-related arm lymphedema: a systematic review and dose-response meta-analysis.

Purposes: This meta-analysis aims to (1) examine how resistance training affects lymphedema and muscle strength; and (2) evaluate the extent of improvement in lymphedema with different exercise dosages in breast cancer patients.

Methods: Eight English and Chinese electronic databases were searched from their inception up to April 7, 2024. A dose-response meta-analysis was performed with R software.

Survival of Listeria monocytogenes on growing and harvested Trumpet Royale (Pleurotus eryngii), Alba Clamshell (Hypsizygus tessellatus), and Brown Clamshell (Hypsizygus tessellatus) mushrooms.

Outbreaks and recalls of Listeria monocytogenes associated with commercial mushrooms have been reported in recent years. Unfortunately, knowledge about the survival of L. monocytogenes on different mushrooms remains limited.

The impact of teacher emotional support on learning engagement among college students mediated by academic self-efficacy and academic resilience.

College students' learning engagement not only significantly influences their academic performance but also plays a vital role in their future career development. Ensuring that students maintain high levels of engagement is essential for society's goal of cultivating high-quality talent. Therefore, understanding the key factors that drive student engagement is critical for educators as they develop effective strategies to foster this engagement.

Non-wounding contact-based Inoculation of fruits with fungal pathogens in postharvest.

Background: Fungal pathogens significantly impact the quality of fruits and vegetables at different stages of the supply chain, leading to substantial food losses. Understanding how these persistent fungal infections occur and progress in postharvest conditions is essential to developing effective control strategies.

Results: In this study, we developed a reliable and consistent inoculation protocol to simulate disease spread from infected fruits to adjacent healthy fruits during postharvest storage.

Microbiome-based classification models for fresh produce safety and quality evaluation.

Unlabelled: Small sample sizes and loss of sequencing reads during the microbiome data preprocessing can limit the statistical power of differentiating fresh produce phenotypes and prevent the detection of important bacterial species associated with produce contamination or quality reduction. Here, we explored a machine learning-based -mer hash analysis strategy to identify DNA signatures predictive of produce safety (PS) and produce quality (PQ) and compared it against the amplicon sequence variant (ASV) strategy that uses a typical denoising step and ASV-based taxonomy strategy. Random forest-based classifiers for PS and PQ using 7-mer hash data sets had significantly higher classification accuracy than those using the ASV data sets.

Protein-Based Rechargeable and Replaceable Antimicrobial and Antifouling Coatings on Hydrophobic Food-Contact Surfaces.

The growing concerns regarding foodborne illnesses related to fresh produce accentuate the necessity for innovative material solutions, particularly on surfaces that come into close contact with foods. This study introduces a sustainable, efficient, and removable antimicrobial and antifouling coating ideally suited for hydrophobic food-contact surfaces such as low-density polyethylene (LDPE). Developed through a crosslinking reaction involving tannic acid, gelatin, and soy protein hydrolysate, these coatings exhibit proper stability in aqueous washing solutions and effectively combat bacterial contamination and prevent biofilm formation.

Upcycling Romaine lettuce outer leaves by infrared blanching and hot air drying.

Romaine lettuce outer leaves, as opposed to the more commonly marketed heart, are typically discarded and present an opportunity for upcycling as dried powders. Duquesne Romaine lettuce was evaluated to quantify and compare quality attributes of fresh outer and heart leaves, dried powders following hot air drying, and dried powders following an infrared (IR) blanching pretreatment before drying. Attributes measured for fresh leaves included moisture, water activity (A), color, total soluble phenolics (TSP), and antioxidant capacity (AC).

Fitness and transcriptomic analysis of pathogenic in seawater at different shellfish harvesting temperatures.

Given the involvement of () in a wide range of seafood outbreaks, a systematical characterization of fitness and transcriptomic changes at temperatures of critical importance for seafood production and storage is needed. In this study, one of each virulent strain (+ and +) was tested. While no difference in survival behavior of the two virulent strains was observed at 10°C, the + strain had a faster growth rate than the + strain at 30°C.

Survival of Listeria monocytogenes and Salmonella in citrus storage waxes or on lemons held under common commercial storage conditions.

To prolong cold storage, diluted storage waxes are applied to washed lemons after harvest and before packing, without drying steps, to reduce premature rotting and water loss. The survival of Listeria monocytogenes and Salmonella in undiluted and diluted storage waxes (S1-S4), and on lemon surfaces under common commercial storage were investigated. Populations of L.

Mechanisms and Performances of Physically and Chemically Crosslinked Gelatin-Based Hydrogels as Advanced Sustainable and Reusable "Jelly Ice Cube" Coolants.

A novel reusable, plastic-free, and stable cooling medium, Jelly Ice Cubes (JIC), is developed based on crosslinked gelatin hydrogels for sustainable temperature control. A novel process involving a rapid-freezing-slow-thawing treatment and a subsequent photo-crosslinking reaction induced by menadione sodium bisulfite, a newly discovered photosensitizer, is able to effectively consolidate a three-dimensional (3-D) hydrogel network to survive repeated application freeze-thaw cycles (AFTCs). This study reveals the mechanisms and evidence of the synergistic effects of the physical and chemical crosslinking reactions.

Enhanced acetate utilization for value-added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis.

The limited supply of reducing power restricts the efficient utilization of acetate in Yarrowia lipolytica. Here, microbial electrosynthesis (MES) system, enabling direct conversion of inward electrons to NAD(P)H, was used to improve the production of fatty alcohols from acetate based on pathway engineering. First, the conversion efficiency of acetate to acetyl-CoA was reinforced by heterogenous expression of ackA-pta genes.

Approaches for a more microbiologically and chemically safe dried fruit supply chain.

Global production of dried fruits has increased significantly in the past decade. Both the increased consumer acceptance of nutritious packaged food and the broad use of dried fruits in products such as confectionery and bakery goods have fueled the dried fruit demand. Unfortunately, outbreaks and recalls due to contamination by pathogenic bacteria and viruses as well as the detection of mycotoxins highlight the need for optimizing current approaches, and evaluating and adopting newer interventions to protect the microbial and chemical safety of dried fruits.

Formation of Listeria monocytogenes persister cells in the produce-processing environment.

Persisters are a subpopulation of growth-arrested cells that possess transient tolerance to lethal doses of antibiotics and can revert to an active state under the right conditions. Persister cells are considered as a public health concern. This study examined the formation of persisters by Listeria monocytogenes (LM) in an environment simulating a processing plant for leafy green production.