DAPK3-Ablation Regulates the AMPK/mTOR-GPX4 Signaling Pathway to Affect Biological Functions of Staphylococcus aureus-Treated Bone Marrow Mesenchymal Stem Cells and Potentially Ameliorate Osteomyelitis.

Staphylococcus aureus (SA)-caused osteomyelitis (OM) is inflammation-related refractory disease that seriously degrades the life quality of human beings. Unfortunately, up until now, the molecular mechanisms of OM progression have not been fully delineated, which hampered the development of treatment strategies for this disease. The present study aimed to resolve this issue and a novel DAPK3/AMPK/mTOR-GPX4 signal pathway was significantly linked to the development of OM.

The efficacy and safety of Chinese herbal medicine Shugan Sanjie decoction in the treatment of uterine fibroids: a systematic review and meta-analysis.

Background: Shugan Sanjie Decoction (SGSJ) is a commonly used Chinese medicine prescription for the treatment of uterine fibroids (UFs). However, there is still a lack of evidence for its effects and safety. To systematically assess the efficacy and safety of SGSJ in conjunction with Mifepristone [MFP] or Leuprolide acetate [LA] for the treatment of UFs, thereby providing a reference for clinical medication.

Dye-doped polymer-coated optical fiber optofluidic laser for refractive index sensing.

In this work, we reported an organic dye-doped polymer-coated optical fiber optofluidic laser (FOFL) that supports whispering gallery mode (WGM) laser emission. The dye-doped polymer was coated on the outer surface of single-mode fibers (SMFs) using a simple method to fabricate a uniform and consistent solid gain film. By using coated fibers as optical microcavities for lasers, a low lasing threshold of about 0.

Efficacy and safety of lateral lumbar interbody fusion for lumbar degenerative diseases with or without auxiliary posterior fixation: a meta-analysis and systematic review.

Background: There is no conclusive evidence from evidence-based medicine that clarifies whether the efficacy and safety of lateral lumbar interbody fusion (LLIF) differ significantly with or without auxiliary posterior fixation. This study embarks on a comprehensive comparative meta-analysis, delving into both domestic and international research landscapes, to scrutinize the efficacy of stand-alone LLIF versus LLIF coupled with auxiliary posterior fixation in the treatment of degenerative lumbar diseases.

Methods: In this meta-analysis, we searched Pubmed, Embase, and Cochrane databases from inception to December 2023.

The Sirt1/FOXO signal pathway involves in regulating osteomyelitis progression via modulating mitochondrial dysfunctions and osteogenic differentiation.

The Sirtuin-1 (Sirt1) gene has been reported to be closely associated with the progression of multiple diseases, but its role in regulating osteomyelitis (OM) pathogenesis has not been explored. The murine long bone-derived osteocyte-like MLO-Y4 cells and osteoblast-like MC3T3-E1 cells were exposed to Staphylococcal protein A (SpA) treatment to establish the in vitro OM models. The expression levels of Osteoblast-specific genes (OCN, OPN and RUNX2), osteoclastic genes (CTSK, MMP9 and ACP5) and the FOXO pathway-related proteins (FOXO1, p-FOXO1, FOXO3 and p-FOXO3) were detected by performing Real-Time qPCR and Western Blot analysis.

An AttSDNet model for multi-scale feature perception enhanced remote sensing classification of coastal salt-marsh wetlands.

Coastal salt-marsh wetlands have important ecological value, and play an important role in coastal blue carbon sink. However, under the influence of various external and natural factors, coastal wetland ecosystems worldwide have severely degraded, leading to biodiversity loss and ecological damage. Based on satellite remote sensing data and deep learning methods, it is an effective means to quickly monitor the spatial distribution of coastal wetlands, which is very important for the protection and restoration of coastal wetlands.

Dual Function of Naphthalenediimide Supramolecular Photocatalyst with Giant Internal Electric Field for Efficient Hydrogen and Oxygen Evolution.

Organic supramolecular photocatalysts have garnered widespread attention due to their adjustable structure and exceptional photocatalytic activity. Herein, a novel bis-dicarboxyphenyl-substituent naphthalenediimide self-assembly supramolecular photocatalyst (SA-NDI-BCOOH) with efficient dual-functional photocatalytic performance is successfully constructed. The large molecular dipole moment and short-range ordered stacking structure of SA-NDI-BCOOH synergistically create a giant internal electric field (IEF), resulting in a remarkable 6.

Generalized Camera-Based Infant Sleep-Wake Monitoring in NICUs: A Multi-Center Clinical Trial.

The infant sleep-wake behavior is an essential indicator of physiological and neurological system maturity, the circadian transition of which is important for evaluating the recovery of preterm infants from inadequate physiological function and cognitive disorders. Recently, camera-based infant sleep-wake monitoring has been investigated, but the challenges of generalization caused by variance in infants and clinical environments are not addressed for this application. In this paper, we conducted a multi-center clinical trial at four hospitals to improve the generalization of camera-based infant sleep-wake monitoring.

A Scene Adaption Framework for Infant Cry Detection in Obstetrics.

Infant cry provides useful clinical insights for caregivers to make appropriate medical decisions, such as in obstetrics. However, robust infant cry detection in real clinical settings (e.g.

A Large-Scale Clinical Benchmark of ResNet-based Deep Models for Newborn Face Recognition.

Newborn face recognition is a meaningful application for obstetrics in the hospital, as it enhances security measures against infant swapping and abduction through authentication protocols. Due to limited newborn face datasets, this topic was not thoroughly studied. We conducted a clinical trial to create a dataset that collects face images from 200 newborns within an hour after birth, namely NEWBORN200.

Selenium nanoparticles coated with polysaccharide-protein complexes from abalone viscera improve growth and enhance resistance to diseases and hypoxic stress in juvenile Nile tilapia (Oreochromis niloticus).

The use of selenium nanoparticles (SeNPs) in aquaculture has been increasing gradually over the past few years. SeNPs enhance immunity, are highly effective against pathogens, and have low toxicity. In this study, SeNPs were prepared using polysaccharide-protein complexes (PSP) from abalone viscera.

Tunable light trapping in the graphene metasurface.

Graphene metasurfaces based on surface plasmon resonance can greatly enhance the interaction between light and matter at the nanoscale. At present, the resonance of graphene metasurfaces is widely used to enhance the absorption of atomic layer graphene, but little work has focused on the light field trapping capabilities it brings. In this paper, we numerically study the light trapping and manipulation of an asymmetric graphene metasurface.

LncRNA LINC00963 promotes osteogenic differentiation of hBMSCs and alleviates osteoporosis progression by targeting miRNA-760/ETS1 axis.

Although long non-coding RNA LINC00963 has been reported to play a crucial regulatory role in osteoporosis (OP), its specific mechanism has not been well studied. Cell viability of human bone marrow mesenchymal stem cells (hBMSCs) transfected with short hairpin RNA targeting LINC00963 (sh-LINC00963) and negative control (sh-NC) was analysed by cell counting kit-8 (CCK-8) assay. Alkaline phosphatase (ALP) activity in hBMSCs transfected with sh-LINC00963 and sh-NC after induction by osteogenic medium (OM) on day 7 was detected.

MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis.

Background: Osteoporosis seriously disturbs the life of people. Meanwhile, inhibition or weakening of osteogenic differentiation is one of the important factors in the pathogenesis of osteoporosis. It was reported that miR-27a-3p reduced the symptoms of osteoporosis.

Euxanthone inhibits traumatic spinal cord injury via anti-oxidative stress and suppression of p38 and PI3K/Akt signaling pathway in a rat model.

Background: Owing to neurite promoting, antioxidant and anti-inflammatory effects of Euxanthone (Eux), the investigation was aimed to probe the neuroprotective efficacy of Eux against traumatic spinal cord injury (t-SCI) in rats and whether Eux can improve neuropathic function in t-SCI.

Method: Sprague-Dawley (SD) rats were randomized in - Sham, t-SCI, Eux30, and Eux60 (t-SCI + 30 and 60 mg/kg respectively). Animals with compression force-induced t-SCI were subjected to estimation of locomotor functions.

Placenta-Derived Osteoprotegerin Is Required for Glucose Homeostasis in Gestational Diabetes Mellitus.

Osteoprotegerin (OPG) is involved in various biological processes, including bone remodeling, vascular calcification and pancreatic β-cell function. Although some clinical studies have shown an increase in serum OPG level during pregnancy, the role of OPG in gestational diabetes mellitus (GDM) is largely unknown. Therefore, we explored the effect of OPG in metabolic homeostasis during pregnancy.

Very-low-dose decitabine treatment for patients with intermediate- or high-risk myelodysplastic syndrome: a retrospective analysis of thirteen cases.

Decitabine is a hypomethylating drug that is used to treat myelodysplastic syndrome (MDS) at a recommended dose and schedule (20 mg/m per day, for 5 consecutive days). However, due to its relatively high incidence of side effects and its effects on neoplastic cells, many studies have begun to explore the clinical application of a low dose of decitabine for treating MDS. In this retrospective study, we examined the effects of a very-low-dose decitabine schedule for treating MDS.

Preparation and growth-promoting effect of selenium nanoparticles capped by polysaccharide-protein complexes on tilapia.

Background: Compared with traditional inorganic and organic selenium compounds, nano-selenium exhibited higher biological safety and nutritional potency. However, the biological efficacy of nano-selenium has not been comprehensively and accurately evaluated due to its dispersion instability.

Results: In this study, novel selenium nanoparticles (SeNPs) with high dispersion stability were successfully prepared using a polysaccharide-protein complex (PSP) as the capping agent.

MicroRNA-92a-3p enhances functional recovery and suppresses apoptosis after spinal cord injury via targeting phosphatase and tensin homolog.

Spinal cord injury (SCI) is a neurological disease commonly caused by traumatic events on spinal cords. MiRNA-92a-3p is reported to be down-regulated after SCI. Our study investigated the effects of up-regulated miR-92a-3p on SCI and the underlying mechanisms.

CMKLR1 deficiency attenuates androgen-induced lipid accumulation in mice.

Excess androgen-induced obesity has become a public health problem, and its prevalence has increased substantially in recent years. Chemokine-like receptor 1 (CMKLR1), a receptor of chemerin secreted by adipose tissue, is linked to adipocyte differentiation, adipose tissue development, and obesity. However, the effect of CMKLR1 signaling on androgen-mediated adiposity in vivo remains unclear.

LINC01534 Promotes the Aberrant Metabolic Dysfunction and Inflammation in IL-1β-Simulated Osteoarthritic Chondrocytes by Targeting miR-140-5p.

Objective: Long non-coding RNA 01534 (LINC01534) is highly expressed in the tissues of patients with osteoarthritis (OA). This study investigated the mechanism of LINC01534 on abnormal metabolic dysfunction in OA chondrocytes induced by interleukin-1β (IL-1β).

Methods: The quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expressions of LINC01534, aggrecan, collagen II, and matrix metalloproteinase (MMPs) in OA cartilage tissue or OA chondrocyte model induced by IL-1β.

First Report of a Case with Nondeletional Hb H Disease Caused by IVS-I-116 (A>G) of the α2-Globin Gene.

In this report, we describe a prenatal case with cardiomegaly and increased middle cerebral artery-peak systolic velocity (MCA-PSV) at 22 weeks' gestation. Fetal blood sampling revealed moderate anemia (Hb 7.4 g/dL) and increased Hb Bart's (γ4) level (28.

Global mRNA and Long Non-Coding RNA Expression in the Placenta and White Adipose Tissue of Mice Fed a High-Fat Diet During Pregnancy.

Background/aims: Gestational diabetes mellitus (GDM) is a common complication of pregnancy, but the mechanisms underlying the disorders remain unclear. The study aimed to identify mRNA and long non-coding RNA (lncRNA) profiles in placenta and gonadal fat of pregnant mice fed a high-fat diet and to investigate the transcripts and pathways involved in the development of gestational diabetes mellitus.

Methods: Deep and broad transcriptome profiling was performed to assess the expression of mRNAs and lncRNAs in placenta and gonadal fat from 3 mice fed an HFD and chow during pregnancy.

Staphylococcus Aureus Induces Osteoclastogenesis via the NF-κB Signaling Pathway.

BACKGROUND Osteomyelitis is one of the refractory diseases encountered in orthopedics, while Staphylococcus aureus (S. aureus) is the most common causative organism in osteomyelitis. However, the precise mechanisms underlying the bone loss caused by S.