Publications by authors named "Linjia Cheng"
Circulating Extracellular Vesicles from Heart Failure Patients Inhibit Human Cardiomyocyte Activities.
J Cardiovasc Transl Res
October 2024
Article Synopsis
- Extracellular vesicles (EVs) play a significant role in heart failure (HF), but the specific impact of circulating EVs (CEVs) in HF is still not fully understood.
- In a study, a comparison between CEVs from heart failure patients (HCEVs) and those from healthy individuals (NCEVs) revealed 6 proteins that were upregulated and 9 that were downregulated in HCEVs, indicating key differences in their biological functions.
- The upregulated proteins in HCEVs were linked to inflammation and metabolic processes, while the downregulated proteins affected cell growth and repair, leading to increased inflammation and impaired regenerative ability in heart cells, ultimately contributing to harmful cardiac remodeling in HF.
Purpose: The distribution and expression level of a protein among animal tissues is indicative of its possible roles. It is important to establish a generally applicable method to prepare protein samples with high-quality and achieve near 100% recovery of proteins from animal tissues.
Experimental Design: During preparation, to sufficiently dissolve and maintain stability of almost all proteins from tissues, as well as to avoid most contaminations affecting protein detection, 2×SDS Sample Buffer, sonication and trichloroacetic acid precipitation are applied.