Histone Methyltransferase EHMT2 Promotes the Progression of Breast Ductal Carcinoma by Regulating the Hippo Pathway.

Invasive ductal carcinoma (IDC) is a major type of breast cancer. The utilization of inhibitors targeting histone methyltransferases introduces novel therapeutic avenues for the treatment of cancer. Immunohistochemistry, Western blot, and reverse transcription quantitative polymerase chain reaction experiments were applied to assess the levels of EHMT2 in IDC and adjacent tissues.

Temporal Regulation of Early-Stage Cytokine Expression in Diabetic Wound Healing Under Negative Pressure Wound Therapy.

Negative pressure wound therapy (NPWT) is widely recognized for its efficacy in treating diabetic wounds, but the mechanisms involved in the wound healing process remain unclear. By examining changes in blood cytokine levels as molecular signaling precursors, we aim to provide a comprehensive cytokine profile to support adjunctive therapy research and clinical applications. A diabetic mouse wound model was established to compare cytokine profiles between NPWT-treated and standard dressing groups, identifying key signaling candidates that may facilitate wound healing.

Development and validation of a pathological model predicting the efficacy of neoadjuvant therapy for breast cancer based on RCB scoring.

Introduction: Breast cancer has become the most prevalent malignant tumor among women globally, posing a serious threat to women's life and health. Neoadjuvant therapy (NAT) has emerged as one of the standard treatment approaches for breast cancer patients. However, due to varying responses to NAT among different patients, significant differences in treatment effectiveness occur, impacting the timely alteration of treatment strategies for patients.

Optomechanical squeezing with strong harmonic mechanical driving.

In this paper, we propose an optomechanical scheme for generating mechanical squeezing over the 3 dB limit, with the mechanical mirror being driven by a strong and linear harmonic force. In contrast to parametric mechanical driving, the linearly driven force shakes the mechanical mirror periodically oscillating at twice the mechanical eigenfrequency with large amplitude, where the mechanical mirror can be dissipatively stabilized by the engineered cavity reservoir to a dynamical squeezed steady state with a maximum degree of squeezing over 8 dB. The mechanical squeezing of more than 3 dB can be achieved even for a mechanical thermal temperature larger than 100 mK.

Efficacy of borneol-gypsum in skin regeneration and pain control in toxic epidermal necrolysis: A case report.

Background: Toxic epidermal necrolysis (TEN) is a life-threatening dermatological emergency mainly induced by drug hypersensitivity reactions. Standard management includes discontinuation of culprit drug and application of immunomodulatory therapy. However, mortality remains high due to complications like septic shock and multiorgan failures.

Research progress on influencing factors affecting the efficacy of moxibustion in treating knee osteoarthritis.

The article summarizes the relevant factors to the therapeutic effect of moxibustion on knee osteoarthritis, including the origin and storage time of moxa leaves, the time of moxibustion, the numbers of moxa cone, and the temperature when moxibustion is operated. in Qichun county stored for over 3 years is the best regarding its property； and it is recommended for about 40 min in suspended moxibustion； and the heat-sensitive moxibustion is determined when the sensation of moxibustion disappears； and in terms of moxibustion techniques and the numbers of moxa cone, two moxa cones are optimal in warm needling, but the highly applicable duration of moxibustion needs to be confirmed through more high-quality studies. There are few studies on the other influencing factors, such as the specific operation of suspended moxibustion, the angle of knee flexion, treatment sequence, light and smoking factors, moxibustion method and disease staging and type； and the studies are limited in the comparison in terms of the middle-term and long-term efficacy, the comparison of the efficacy among different syndromes of traditional Chinese medicine in patients and the comparison among various frequencies and sessions of treatment.

Deciphering Early-Stage Molecular Mechanisms of Negative Pressure Wound Therapy in a Murine Model.

Negative Pressure Wound Therapy (NPWT) is a commonly employed clinical strategy for wound healing, yet its early-stage mechanisms remain poorly understood. To address this knowledge gap and overcome the limitations of human trials, we establish an NPWT C57BL/6JNarl mouse model to investigate the molecular mechanisms involved in NPWT. In this study, we investigate the intricate molecular mechanisms through which NPWT expedites wound healing.

Ligand Phase Separation-Promoted, "Squeezing-Out" Mode Explaining the Mechanism and Implications of Neutral Nanoparticles That Escaped from Lysosomes.

Neutral nanomaterials functionalized with PEG or similar molecules have been popularly employed as nanomedicines. Compared to positive counterparts that are capable of harnessing the well-known proton sponge effect to facilitate their escape from lysosomes, it is yet unclear how neutral substances got their entry into the cytosol. In this study, by taking PEGylated, neutral Au nanospheres as an example, we systematically investigated their time-dependent translocation postuptake.

Quality consistency evaluation of commercial Prunellae Spica by integrating determination of secondary metabolites and saccharides.

Introduction: Prunellae Spica (PS) is a commonly used medicinal herb in China. Secondary metabolites and saccharides are major bioactive components of PS. However, holistic quality consistency of commercial PS is ambiguous due to lack of comprehensive evaluation methods and reliable quality control markers.

Genetic Disruption of KLF1 K74 SUMOylation in Hematopoietic System Promotes Healthy Longevity in Mice.

The quest for rejuvenation and prolonged lifespan through transfusion of young blood has been studied for decades with the hope of unlocking the mystery of the key substance(s) that exists in the circulating blood of juvenile organisms. However, a pivotal mediator has yet been identified. Here, atypical findings are presented that are observed in a knockin mouse model carrying a lysine to arginine substitution at residue 74 of Krüppel-like factor 1 (KLF1/EKLF), the SUMOylation-deficient Klf1 mouse, that displayed significant improvement in geriatric disorders and lifespan extension.

Characteristics of Dye-Sensitized Solar Cells with TiO Stripes.

A TiO strip array with a thickness of 90 nm was fabricated by photolithography and physical vapor deposition. This work utilized the chemical and physical methods to fabricate the TiO strip array. A porous semiconductor layer made of TiO nanoparticles was coated on the TiO strip array.

Inhibition of CDK1 attenuates neuronal apoptosis and autophagy and confers neuroprotection after chronic spinal cord injury in vivo.

Chronic spinal cord injury (CSCI) results from progressive compression of the spinal cord over time. A variety of factors cause CSCI, and its exact pathogenesis is unknown. Cyclin-dependent kinase 1 (CDK1) is closely related to the apoptosis pathway, but no CSCI-related studies on CDK1 have been conducted.

Primary localized gastric amyloidosis: A scoping review of the literature from clinical presentations to prognosis.

Localized gastric amyloidosis (LGA) is a rare disease characterized by abnormal extracellular deposition of amyloid protein restricted to the stomach and it is confirmed by positive results of Congo red staining. Over decades, only a few cases have been reported and studies or research focusing on it are few. Although LGA has a low incidence, patients may suffer a lot from it and require proper diagnosis and management.

Improving macroscopic entanglement with nonlocal mechanical squeezing.

We report an efficient mechanism to generate mechanical entanglement in a two-cascaded cavity optomechanical system with optical parametric amplifiers (OPAs) inside the two coupled cavities. We use the especially tuned OPAs to squeeze the hybrid mode composed of two mechanical modes, leading to strong macroscopic entanglement between the two movable mirrors. The squeezing parameter as well as the effective mechanical damping are both modulated by the OPA gains.

Experimental Study on Microfluidic Mixing with Different Zigzag Angles.

This paper presents experimental investigations of passive mixing in a microfluidic channel with different zigzag angles. Zigzag channel is commonly used for microfluidic mixing because it does not need an additional control unit and can be easily implemented in a lab-on-a-chip system. In this work, microfluidic channels with six different zigzag angles, from θ = 0° to θ = 75°, are tested under ten different flow rates corresponding to Reynolds number from 0.

[IL-18 and NLRP3 in the Process of Acnevulgaris].

Objective: To determine the role of inlerleukin-18 (IL-18) and nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) in acne vulgaris.

Methods: We used suspensions [multiplicity of infection (MOI)=0, 10, 20, 30] to stimulate normal human epidermal kerationocytes (NHEKs) for 12, 24, and 36 h, respectively. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the protein level of IL-18.

Isolation of blue-green eggshell pigmentation-related genes from Putian duck through RNA-seq.

Background: The diversity of avian eggshell colour plays important biological roles in ensuring successful reproduction. Eggshell colour is also an important trait in poultry, but the mechanisms underlying it are poorly understood in ducks. This study aimed to provide insights into the mechanism of blue-green eggshell colour generation.

Apoptosis of mouse myeloma cells induced by curcumin via the Notch3-p53 signaling axis.

Resistance to apoptosis is a characteristic of cancer. Curcumin has become a potential anticancer drug for its pro-apoptotic effects, but the underlying mechanisms remain unclear. Furthermore, the Notch3-p53 signaling axis serves an important role in cell fate.

Theranostics Aspects of Various Nanoparticles in Veterinary Medicine.

Nanoscience and nanotechnology shows immense interest in various areas of research and applications, including biotechnology, biomedical sciences, nanomedicine, and veterinary medicine. Studies and application of nanotechnology was explored very extensively in the human medical field and also studies undertaken in rodents extensively, still either studies or applications in veterinary medicine is not up to the level when compared to applications to human beings. The application in veterinary medicine and animal production is still relatively innovative.

[Treatment of scapular fractures with lateral incision and bridge combined internal fixation system].

Objective: To investigate the clinical results of scapula fractures with lateral incision combined with bridge internal fixation system.

Methods: From October 2012 to December 2016, 20 cases of scapular fractures were treated through the lateral incision combined with bridge fixation system, including 15 males and 5 females, with an average age of 31.6 years old(ranged, 21 to 52 years old).

Co-expression of Dorsal and Rel2 Negatively Regulates Antimicrobial Peptide Expression in the Tobacco Hornworm Manduca sexta.

Nuclear factor κB (NF-κB) plays an essential role in regulation of innate immunity. In mammals, NF-κB factors can form homodimers and heterodimers to activate gene expression. In insects, three NF-κB factors, Dorsal, Dif and Relish, have been identified to activate antimicrobial peptide (AMP) gene expression.