Superchaotropic Stabilization of Monomeric Protein States.

Chaotropes are long known to destabilize protein assemblies and folding. We report that a boron cluster ion, as a weakly coordinating superchaotrope, can paradoxically stabilize protein folding even under extended thermal stresses while broadly inhibiting specific and nonspecific protein-protein interactions at millimolar concentrations for multiple proteins. Thermodynamic and kinetic investigations suggest that the boron cluster ion reduced the association rates of protein association and rendered protein-associative interactions entropically unfavorable.

ProMUS-NET: Artificial intelligence detects more prostate cancer than urologists on micro-ultrasonography.

Objectives: To improve sensitivity and inter-reader consistency of prostate cancer localisation on micro-ultrasonography (MUS) by developing a deep learning model for automatic cancer segmentation, and to compare model performance with that of expert urologists.

Patients And Methods: We performed an institutional review board-approved prospective collection of MUS images from patients undergoing magnetic resonance imaging (MRI)-ultrasonography fusion guided biopsy at a single institution. Patients underwent 14-core systematic biopsy and additional targeted sampling of suspicious MRI lesions.

Self-powered deep-ultraviolet photodetector based on a perovskite/oxide heterojunction.

Deep-ultraviolet photodetectors have promising applications in both civil and military fields, due to the advantage of effective light response without an external power supply. In this work, we proposed a self-powered deep-ultraviolet photodetector based on a p-CsCuI/n-ZnGaO heterojunction fabricated by a pulsed laser deposition technique. Under 260 nm illumination and 0 V bias, the photodetector exhibited a maximum responsivity and specific detectivity of 1.

Temperature-Modulated Eu-Doped GdO Cataluminescence Sensing Platform for Selective Detection of Isomeric and Homologous Alcohols.

The selective and rapid identification of alcohol vapors, including isomers and homologues, is crucial for environmental monitoring and industrial safety. Herein, a high-performance cataluminescence (CTL) sensing platform based on Eu-doped GdO was developed to discriminate alcohol vapors. Temperature-modulated CTL responses were employed to encode both thermodynamic and kinetic information, generating distinctive molecular fingerprints for tested alcohols to enable effective substance differentiation.

Regulatory Plasticity and Metabolic Trade-offs Drive Adaptive Evolution of Alternative Flagellar Configurations in .

Evolutionary constraints governing flagellar number in bacterial pathogens remain poorly understood. While related species are hyperflagellated, maintains strict monoflagellation through the FleQ-FleN regulatory circuit. Here, we demonstrate that FleN dosage is essential for maintaining monoflagellation and bacterial fitness.

Bird-Inspired Time-Domain Analysis: High-Robustness Intelligent Sensing of Hypochlorite Aerosols.

The increasing demand for highly reliable sensors capable of anti-interference and sensitive detection of aerosol-associated hypochlorite (ClO) drives progress in luminescence-based sensing technologies. Inspired by collective behavioral patterns in bird flocks, we developed a "bird-like flock" time-domain sensing method through the utilization of functional long afterglow nanomaterials (AGNPs) for ClO detection. Particularly, by translating avian aggregation-separation-alignment behaviors into an algorithmic framework, we presented a new sensing mode based on simplifying time decay curves into interpretable temporal metrics.

Engineered Membrane Vesicle Production via or Deletion Has Distinct Phenotypic Effects in .

Membrane vesicle (MV) production is a natural phenomenon in Gram-negative bacteria and represents an emerging synthetic biology tool for the secretion of biomolecules or bioproducts. Manipulation of membrane components has proven successful in enhancing MV production. However, the impact of membrane disruptions on strain fitness and protein composition warrants further investigation for the use of MVs in industrial bioprocesses.

Inhibited the Proliferation of Sheep Dermal Papilla Cells by Inhibiting the Expression of .

The proliferation and maturation of hair follicles in follicular papilla cells are predominantly governed by miRNAs, which significantly influence the cell cycle, apoptosis, and proliferation. has been associated with several biological processes and targets , a crucial component in hair follicle development. Tissue expression profiling revealed significant differences in levels between skin tissues of the two sheep breeds in January and October, as well as between tissues of the Xinji fine-wool sheep and Small-tail Han sheep.

Single nanoparticle analysis-based CRISPR/Cas12 bioassay for amplification-free HIV detection.

To reduce the "window period" in HIV detection, most analytical methods require additional enzymes for signal amplification. Exempting challenges like primer interference and false positives in amplification strategies, we developed an amplification-free bioassay that uses CRISPR's potent cleavage activity and the competent sensitivity of single-nanoparticle analysis. An attomolar detection limit was achieved with adequate selectivity.

Can Mismatch Negativity Be Used as an Indicator to Predict Central Auditory Deficits in Individuals with Normal Hearing?

: In the early stage of presbycusis, patients experience reduced speech perception in noisy environments despite normal audiometry, normally known as hidden hearing loss. Diagnostic indicators like the reduced amplitude of ABR wave I, elevated extended high-frequency threshold (EHT), and decreased middle ear muscle reflex (MEMR) amplitude aim to identify biomarkers of peripheral auditory pathology but remain inconsistent. Mismatch negativity (MMN) is a cortical auditory evoked potential generated when the brain detects sound changes.

Preventive administration of shengmaiyin: a novel approach to counteract heatstroke-induced coagulopathy in rats.

Background: Coagulation disorders play a pivotal role in the elevated mortality rates associated with exertional heatstroke (EHS).

Purpose: To investigate the impact of Shengmai Yin Oral Liquid (SMY) on heatstroke-induced coagulopathy (HIC) in rats with EHS and to elucidate the underlying mechanisms.

Methods: A cohort of eighteen male SPF-grade SD rats, each implanted with a telemetric temperature capsule, were randomly allocated to three groups: a normal control (NC) group, an EHS group, and an SMY group (n = 6 per group).

Novel Pyrolysis-Assisted Cataluminescence System for Fingerprint Discrimination of Various Microplastics.

As emerging pollutants, microplastics are pervasive in marine, terrestrial, and atmospheric environments as well as human tissues due to their small size, large specific surface area, and strong adsorption capacity. Consequently, developing efficient and rapid methods for identifying and differentiating microplastics is critical. Herein, a novel pyrolysis-assisted cataluminescence (CTL) sensor array was constructed, capable of efficiently distinguishing seven distinct types of microplastic samples.

Electrically evoked mismatch negativity from speech stimuli as an objective measure of cochlear implant performance.

Introduction: Cochlear implant (CI) success is often assessed using subjective tests like word recognition scores (WRS). However, these tests are unsuitable for children, non-native speakers, and individuals with cognitive impairments. Mismatch negativity (MMN), an objective measure of cortical auditory processing, offers a promising alternative for evaluating speech perception.

Rapid Classification of OVOC Samples through a Dynamic Cataluminescence Sensor Coupled with a Code-Triggered Global Plasma-Activated System.

Integrated cataluminescence (CTL) sensing for both identification and quantification has become the focus in gas-sensing methods. With the development of an integrated circuit, inspiration from electronic automation provided a convenient approach for the application of CTL sensors. Here, a global dielectric barrier discharge plasma system supported by a digital code is raised for the process-oriented sampling and gaseous dynamic dual-route reaction for the rapid quantitative and qualitative analysis of oxidative volatile organic compounds (OVOCs).

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.

Biological systems are complex, encompassing intertwined spatial, molecular and functional features. However, methodological constraints limit the completeness of information that can be extracted. Here, we report the development of INSIHGT, a non-destructive, accessible three-dimensional (3D) spatial biology method utilizing superchaotropes and host-guest chemistry to achieve homogeneous, deep penetration of macromolecular probes up to centimeter scales, providing reliable semi-quantitative signals throughout the tissue volume.

The / Axis Affects Adipogenesis Through Regulating the / Pathway.

Understanding the regulatory mechanisms of adipogenesis is essential for preventing obesity. Interleukin-33 (IL-33) has recently attracted increasing attention for its role in adipogenesis. The purpose of this study was to explore the function and regulatory mechanism of IL-33 and its receptor suppression of tumorigenicity 2 (ST2) on adipogenesis.

Otogenic Brain Abscess and Concomitant Acute COVID-19 Infection: Case Report and Review of the Literature.

2019 coronavirus disease (COVID-19) has attracted global attention primarily because of the severe acute respiratory symptoms associated with it. However, nearly one third of the patients also present with neurological symptoms. This report describes a case of a previously healthy woman with acute COVID-19 infection, who developed acute facial nerve palsy and rapid progression to coma due to otogenic brain abscess.

Mechanisms of Polyethylene Terephthalate Pellet Fragmentation into Nanoplastics and Assimilable Carbons by Wastewater .

bacteria are enriched on poly(ethylene terephthalate) (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting in a 3.

Analysis of the Long Non-Coding and Messenger RNA Expression Profiles in the Skin Tissue of Super Merino and Small-Tailed Han Sheep.

Wool quality and yield are two important economic livestock traits. However, there are relatively few molecular studies on lncRNA for improving sheep wool, so these require further exploration. In this study, we examined skin tissue from the upper scapula of Super Merino (SM) and Small-Tailed Han (STH) sheep during the growing period.

The Predictive Value of Preoperative Measurements of Cochlear Nerve Diameters From MRT and Postoperative Speech Perception in Adult Patients With Cochlear Implant.

Objective: The current study aims to investigate whether objective measurements of the cochlear nerve (CN), derived from preoperative MRI images, correlate with postoperative speech perception in CI patients.

Study Design: Retrospective cohort study.

Setting: University Medical Center, tertiary academic referral center.

GLIDR-mediated regulation of tumor malignancy and cisplatin resistance in non-small cell lung cancer via the miR-342-5p/PPARGC1A axis.

Background: Lung cancer, particularly non-small cell lung cancer (NSCLC), remains a significant cause of cancer-related mortality, with drug resistance posing a substantial obstacle to effective therapy. LncRNAs have emerged as pivotal regulators of NSCLC progression, suggesting potential targets for cancer diagnosis and treatment. Therefore, identifying new lncRNAs as therapeutic targets and comprehending their underlying regulatory mechanisms are crucial for treating NSCLC.

Inter-reader Agreement for Prostate Cancer Detection Using Micro-ultrasound: A Multi-institutional Study.

Background And Objective: Micro-ultrasound (MUS) uses a high-frequency transducer with superior resolution to conventional ultrasound, which may differentiate prostate cancer from normal tissue and thereby allow targeted biopsy. Preliminary evidence has shown comparable sensitivity to magnetic resonance imaging (MRI), but consistency between users has yet to be described. Our objective was to assess agreement of MUS interpretation across multiple readers.