Antimicrobial resistance profiles of spp. and isolated from dogs and cats in Seoul, South Korea during 2021-2023.

We investigated the antimicrobial resistance (AMR) rates and profiles of spp. and () isolated from dogs and cats in Seoul, South Korea during 2021-2023. We analyzed AMR profiles of spp.

Complete mitochondrial genome sequencing and phylogenetic analysis of the Light-vented Bulbul () killed by window collision in South Korea in 2023.

The Light-vented Bulbul () has rapidly expanded across East Asia, particularly in South Korea since the early 2000s. This study presents the complete mitochondrial genomes of five individuals died from window collisions in Seoul. The genomes, sequenced using next-generation sequencing technology, are 16,923bp long and contain 13 protein-coding genes, 22 tRNAs, and 2 rRNAs.

Genomic evidence for interserovar transfer and evolution of bla carrying plasmid in multidrug-resistant Salmonella Enteritidis and Salmonella Virchow isolated in South Korea.

Multidrug-resistant (MDR) Salmonella enterica serotype Enteritidis (SE) carrying bla has emerged in South Korea; however, the genetic characteristics and evolutionary dynamics of the plasmids remain understudied. This study aimed to characterize the structure, diversity, and evolution of bla-carrying plasmids in SE strains from human and animal sources in South Korea. Plasmid structures of bla-positive SE strains from South Korea were analyzed using incompatibility groups typing and genome alignment tools.

Emergence of Distinct Salmonella enterica Serovar Enteritidis Lineage since 2020, South Korea.

We analyzed whole-genome sequences of Salmonella enterica serovar Enteritidis isolates in South Korea that had the SEGX01.049 pulsed-field gel electrophoresis pattern. That lineage has emerged and circulated in South Korea since 2020, leading to 2 fatal infection cases.

Spatial and genomic profiling of residual breast cancer after neoadjuvant chemotherapy unveil divergent fates for each breast cancer subtype.

Residual cancer burden (RCB) is a strong prognostic marker after neoadjuvant chemotherapy (NAC) in breast cancer (BC), yet some BCs defy their predicted outcomes. Using single-cell spatial transcriptomics and genomic profiling, we investigate mechanisms underlying divergent fates of BCs with high RCB across subtypes. In triple-negative BC (TNBC), CXCL9+ macrophage-CD8 T cell interactions via chemokines and interferon-gamma signaling promote favorable outcomes, while SPP1+ macrophage-cancer cell interactions driven by hypoxia signaling correlate with poor prognosis.

Whole-genome sequencing analysis of serotype Enteritidis isolated from poultry sources in Mongolia.

serotype Enteritidis ( Enteritidis) is a leading foodborne pathogen associated with poultry products, and the emergence of antimicrobial resistance (AMR) in this serotype poses a growing public health concern, particularly in regions with increasing poultry trade. Between April and June 2024, we collected 114 poultry meat samples (Mongolian domestic and Chinese imported) from retail markets in Mongolia and isolated 45 Enteritidis strains. Antimicrobial susceptibility testing revealed high resistance rates to nalidixic acid (100%), ampicillin (93.

Clonal dissemination and persistence of Carbapenem-resistant harbouring ST307 in a Tertiary Hospital in the Republic of Korea.

Objectives: We investigated the epidemiological patterns of carbapenem-resistant, harbouring () ST 307 isolates in a tertiary hospital in South Korea between 2019 and 2021 and compared them to isolates collected from 25 hospitals through a nationwide survey, using whole genome sequencing and phylogenetic analysis.

Methods: Whole genome sequences of 25 isolates from hospital GB2 and 45 isolates from 25 other hospitals were analysed for antibiotic resistance gene profiles, virulence gene profiles, plasmid replicons, and phylogenetic relationships.

Results: Core genome single nucleotide polymorphism (cgSNP)-based phylogenetic analysis categorized the 70 isolates into three distinct groups and 13 subgroups, supported by high bootstrap values and low SNP differences.

Molecular epidemiology of West Nile Virus in raptors, Connecticut, USA, 2022: A case series with whole genome sequencing and phylogenetic analysis.

West Nile virus (WNV), a mosquito-borne flavivirus, circulates in an enzootic cycle between birds and mosquitoes, with raptors serving as key amplifying hosts. Despite their importance in WNV surveillance, complete genome sequences from raptors remain limited. This study aimed to investigate the genetic diversity and evolutionary history of WNV in raptors from Connecticut, USA.

Impact of Therapeutic Radioactive Iodine on the Recurrence of Papillary Thyroid Cancer With Extranodal Extension in Metastatic Lymph Nodes: A Propensity Score-matched Analysis.

Purpose: Radioactive iodine therapy (RAI) ≥100 mCi (3.7 GBq) is commonly recommended for papillary thyroid cancer (PTC) patients with extranodal extension (ENE). However, no study has evaluated whether RAI ≥100 mCi is effective in reducing the recurrence of PTC with ENE.

Subclassifying "Atypia of Undetermined Significance (AUS)" Category in the 2023 Bethesda System for Thyroid Cytopathology: Analyzing K-TIRADS, BRAF V600E Mutation, and Risk of Malignancy.

The 2023 Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) update refines the atypia of undetermined significance (AUS) category by subclassifying it into AUS-nuclear atypia (AUS-N) and AUS-other atypia (AUS-O) based on risk of malignancy, to enhance clarity of diagnosis and patient management. This study evaluates the impact of AUS subclassification on malignancy risk prediction in thyroid nodules. A total of 118 AUS cases were analyzed for subclassification using the nuclear scoring (NS) system to evaluate nuclear features, along with the ultrasonography-based Korean Thyroid Imaging Reporting and Data System (K-TIRADS) for risk stratification, and BRAF V600E mutation testing.

Antibiotic efficacy and resistance patterns of urinary tract infection-causing bacteria in dogs and resistome of multidrug-resistant via whole genome sequencing in South Korea.

Bacterial urinary tract infections (UTIs) are prevalent in dogs and necessitate antibiotic intervention. However, the emergence of multidrug-resistant (MDR) bacteria poses significant challenges to antibiotic therapy. Although fosfomycin has been demonstrated to achieve and maintain high concentrations in urine, suggesting its potential for treating UTIs in dogs, its efficacy and the resistance profiles of urinary pathogens from canine UTIs remain elusive.

Clock Gene Expression in Eel Retina and Hypothalamus: Response to Photoperiod and Moonlight.

Assessment of the clock genes, Period (Per) 1, Per2, Per3, and Cryptochrome (Cry) 2, Cry3, and Cry4, can help better understand eel spawning ecology. In this study, the circadian rhythm and moonlight effects of these clock genes in the eel retina and hypothalamus were analyzed. We examined clock gene expression patterns under 12 h light:12 h darkness (12L12D), constant darkness (DD), and constant light (LL) conditions; under short photoperiod (SP; 9L15D) and long photoperiod (LP; 15L9D), and during the new moon (NM) and full moon in male eels.

Genome analysis of Streptococcus spp. isolates from animals in pre-antibiotic era with respect to antibiotic susceptibility and virulence gene profiles.

Lyophilized Streptococcus spp. isolates (n = 50) from animal samples submitted to the diagnostic laboratory at the University of Connecticut in the 1940s were revivified to investigate the genetic characteristics using whole-genome sequencing (WGS). The Streptococcus spp.

The complete coding sequence of a rabies lyssavirus (RABV) detected in an American black bear () in Connecticut, USA.

The complete coding sequence of a rabies lyssavirus (RABV) detected in a black bear () was generated. RNA extracted from brain tissues was amplified using reverse transcription followed by tiling PCR sequencing to obtain RABV whole viral genome. Sequencing was performed using an Illumina ISeq 100 instrument.

Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica.

Japanese eel (Anguilla japonica) is a commercially important fish species in Asia. Understanding factors like photoperiod, temperature, and lunar cycles is crucial for successful aquaculture and managing its reproduction. Melatonin and dopamine (DA) are essential for regulating reproduction in vertebrates, including fish.

Whole genome sequencing and phylogenetic analysis of West Nile viruses from animals in New England, United States, 2021.

West Nile virus is a mosquito-borne Flavivirus which is the leading cause of global arboviral encephalitis. We sequenced WNVs from an American crow found in Connecticut and an alpaca found in Massachusetts which were submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL). We report here the complete protein-coding sequences (CDS) of the WNVs (WNV 21-3957/USA CT/Crow/2021 and WNV 21-3782/USA MA/Alpaca/2021) and their phylogenetic relationship with other WNVs recovered from across the United States.

Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019.

African swine fever (ASF) is a highly contagious and fatal disease affecting domestic and wild pigs caused by the African swine fever virus (ASFV). Since the first outbreak in China in August 2018, ASF has spread rapidly in Asia. and the first case in Mongolia was confirmed in January 2019.

DNA barcoding and morphological identification of spiny lobsters in South Korean waters: a new record of and .

To date, 19 species of spiny lobsters from the genus have been discovered, of which only , , , and have been documented in South Korean waters. In this study, we aimed to identify and update the current list of spiny lobster species that inhabit South Korean waters based on the morphological features and the phylogenetic profile of cytochrome oxidase I (COI) of mitochondrial DNA (mtDNA). Spiny lobsters were collected from the southern and eastern coasts of Jeju Island, South Korea.

Whole Genome Sequencing and Phylogenetic Analysis of Rabies Viruses from Bats in Connecticut, USA, 2018-2019.

We performed whole genome sequencing and genetic characterization of rabies viruses (RABV) detected in bats submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (CVMDL) during 2018-2019. Among 88 bats submitted to CVMDL, six brain samples (6.8%, 95% confidence interval: 1.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021.

We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology.

Genomic Features of Subspecies Serotype 45:g,z51:- Isolated from Multiple Abdominal Abscesses of an African Fat-Tailed Gecko, United States, 2020.

subsp. () is a common subspecies in reptiles and has been implicated as a source of serious and life-threatening diseases in humans. Although occurrence and significance of infections have been extensively studied, the genetic features of have remained unknown due to a lack of available high-quality genome sequences.