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The complete coding sequence of a rabies lyssavirus (RABV) detected in a black bear () was generated. RNA extracted from brain tissues was amplified using reverse transcription followed by tiling PCR sequencing to obtain RABV whole viral genome. Sequencing was performed using an Illumina ISeq 100 instrument.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927680 | PMC |
http://dx.doi.org/10.1128/MRA.00821-23 | DOI Listing |
JMIR Res Protoc
September 2025
Center for Alcohol & Addiction Studies, School of Public Health, Brown University, Providence, RI, United States.
Background: Digital media frequently contains positive portrayals of alcohol content, which has been shown to be associated with alcohol-related cognitions and behaviors. Because youth are heavy media consumers and have access to unsupervised, repeat viewing of media content on their personal mobile devices, it is critical to understand the frequency of encountering alcohol content in adolescents' daily lives and how adolescents engage with the content.
Objective: This paper outlines the study protocol for examining adolescents' exposure to alcohol-related content in digital media within their natural environments.
J Virol
September 2025
Laboratory of Virology, Wageningen University & Research, Wageningen, the Netherlands.
Vertebrate animals and many small DNA and single-stranded RNA viruses that infect vertebrates have evolved to suppress genomic CpG dinucleotides. All organisms and most viruses additionally suppress UpA dinucleotides in protein-coding RNA. Synonymously recoding viral genomes to introduce CpG or UpA dinucleotides has emerged as an approach for viral attenuation and vaccine development.
View Article and Find Full Text PDFJ Genet
September 2025
College of Life Sciences, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
The family Syngnathidae includes seahorses, sea dragons, and pipefishes. We sequenced the complete mitochondrial DNA (mtDNA) genome of the belly pipefish, Bleeker, 1849. The genome is 16,646-bp long, and includes the standard complement for bony fishes of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region, in the same order and strand distribution as other syngnathids.
View Article and Find Full Text PDFMicrobiol Resour Announc
September 2025
All-Russia Research Institute for Agricultural Microbiology (ARRIAM), St.Petersburg, Russia.
We report the genome sequence of strain AX7B, isolated from the pea rhizosphere and capable of utilizing abscisic acid as a sole carbon source. The complete genome consists of a 6.62 Mb chromosome and a 0.
View Article and Find Full Text PDFJ Genet
September 2025
The Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
The complete mitogenome of the common Chinese whip scorpion, (Butler, 1872) was sequenced and compared with another Uropygid mitogenome of (Lucas, 1835). Structural divergences include the absence of one tRNA-Leu and strand inversions in four protein coding genes (PCGs). All PCGs showed K/K ratios-1, which indicates purifying selection, with COI (0.
View Article and Find Full Text PDF