End-of-Season Influenza Vaccine Effectiveness Against Laboratory-Confirmed Influenza in Outpatient Settings, Beijing, China: A Test-Negative Design.

This study aimed to estimate the end-of-season influenza vaccine effectiveness (VE) for the 2024/25 season in Beijing, China. We used a test-negative design (TND) to assess influenza VE among outpatients with influenza-like illness (ILI) enrolled through the influenza virological surveillance in sentinel hospitals in Beijing from week 44, 2024 to week 14, 2025. Cases were ILI patients who tested positive for influenza; controls were those who tested negative.

Photoexcited CRY1 physically interacts with ATG8 to regulate selective autophagy of HY5 and photomorphogenesis in Arabidopsis.

Cryptochromes (CRYs) are blue light photoreceptors that regulate various light responses in plants, including photomorphogenesis. Autophagy is a tightly controlled intracellular degradation pathway that plays a critical role in plant growth and development. CRY signaling inhibits the 26S proteasome-dependent degradation of LONG HYPOCOTYL 5 (HY5) through interactions with the CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-SUPPRESSOR OF PHYA-105 1 (SPA1) complex.

Sensitive dual detection of N-nitrosodiphenylamine and N-nitrosodiethylamine using a PtNPs-enhanced MWCNTs/Fe-porphyrinic MOF nanocomposite-based electrochemical sensor.

N-nitrosamines, such as N-nitrosodiphenylamine (NDPhA) and N-nitrosodiethylamine (NDEA), are potent carcinogens frequently detected in food and water, posing significant health risks. This study presents a novel non-enzymatic electrochemical sensor using platinum nanoparticles (PtNPs) electrodeposited on a composite of multi-walled carbon nanotubes (MWCNTs)/iron-porphyrinic metal-organic framework (MOF) PCN-600 nanocomposite for the sensitive and simultaneous detection of NDPhA and NDEA. The nanocomposite was synthesized via a solvothermal method, MWCNTs in the nanocomposite prevented MOF aggregation and enhanced conductivity.

Microbiome analysis for artificially establishing the symbiotic relationship between Hebeloma hiemale and Quercus mongolica.

Ectomycorrhizae (ECM) play a critical role in enhancing plant growth and health. However, the influence of artificially established ectomycorrhizal symbioses on the structure and function of rhizosphere microbial communities remains inadequately understood. In this study, a symbiotic relationship between Hebeloma hiemale and Quercus mongolica was established to investigate the influence of ECM on soil microbial communities in the rhizosphere of the host plant.

A MOF-818-based nanozyme-linked aptasensor for microcystin-LR detection in edible algae powder.

The development of simple, rapid, and sensitive detection methods for microcystin-leucine-arginine (MC-LR) is crucial for ensuring food safety. A novel nanozyme-linked colorimetric aptasensor was developed using metal-organic framework (MOF) material MOF-818. The aptamer and MOF-818 with complementary DNA (cDNA) on its surface (MOF-818/cDNA) served as bioreceptors and signal reporters, respectively.

External Phosphate Binding Triggered Formation of Coordination Bridged Dimer of Macrocyclic Trinuclear Complex.

Macrocyclic complexes with spatially-arranged coordination sites have the potential to create unique supramolecular systems that combine the precise switching of self-assembled structures with the coordinative recognition of specific molecules. We now report a macrocyclic trinuclear Zn complex that undergoes a unique dimerization by molecular binding from the outside. The multinuclear complex was synthesized from a trispap ligand, a designed macrocyclic trimer of an N,N,O-type tridentate chelating unit pap.

An evaluation of Astragali Radix with different growth patterns and years, based on a new multidimensional comparison method.

Introduction: With the depletion of wild Astragali Radix (WA) resources, imitated-wild Astragali Radix (IWA) and cultivated Astragali Radix (CA) have become the main products of Astragali Radix. However, the quality differences of three growth patterns (WA, IWA, CA) and different growth years of Astragali Radix have not been fully characterized, leading to a lack of necessary scientific evidence for their use as substitutes for WA.

Methods: We innovatively proposed a multidimensional evaluation method that encompassed traits, microstructure, cell wall components, saccharides, and pharmacodynamic compounds, to comprehensively explain the quality variances among different growth patterns and years of Astragali Radix.

Adaptive Fusion of Deep Learning With Statistical Anatomical Knowledge for Robust Patella Segmentation From CT Images.

Kneeosteoarthritis (KOA), as a leading joint disease, can be decided by examining the shapes of patella to spot potential abnormal variations. To assist doctors in the diagnosis of KOA, a robust automatic patella segmentation method is highly demanded in clinical practice. Deep learning methods, especially convolutional neural networks (CNNs) have been widely applied to medical image segmentation in recent years.

Developing rational scale-down simulators for mimicking substrate heterogeneities based on cell lifelines in industrial-scale bioreactors.

The influence of extracellular variations on the cellular metabolism and thereby the process performance at large-scale can be evaluated using the so-called scale-down simulators. Nevertheless, the major challenge is to design an appropriate scale-down simulator, which can accurately mimic the cell lifelines that record the flow paths and experiences of cells circulating in large-scale bioreactors. To address this, a dedicated SDSA (scale-down simulator application) was purposedly developed on the basis of black box model and process reaction model established for Penicillium chrysogenum strain as well as cell lifelines or trajectories information in an industrial-scale fermentor.

Acidithiobacillus species drive the formation of ferric-silica cemented microstructure: Insights into early hardpan development for mine site rehabilitation.

Hardpan-based profiles naturally formed under semi-arid climatic conditions have substantial potential in rehabilitating sulfidic tailings, resulting from their aggregation microstructure regulated by Fe-Si cements. Nevertheless, eco-engineered approaches for accelerating the formation of complex cementation structure remain unclear. The present study aims to investigate the microbial functions of extremophiles on mineral dissolution, oxidation, and aggregation (cementation) through a microcosm experiment containing pyrites and polysilicates, of which are dominant components in typical sulfidic tailings.

[Nature-effect transformation mechanism of mulberry leaves and silkworm droppings based on chemical composition analysis].

Starting with the relationship between mulberry leaves and silkworm droppings as food and metabolites, this study systematically compared the chemical components, screened out differential components, and quantitatively analyzed the main differential components based on ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and UPLC-Q-TRAP-MS combined with principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA). Moreover, the in vitro enzymatic transformation of the representative differential components was studied. The results showed that(1) 95 components were identified from mulberry leaves and silkworm droppings, among which 27 components only exist in mulberry leaves and 8 components in silkworm droppings.

A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns.

Metal-organic frameworks (MOFs) have attracted significant research interest in biomimetic catalysis. However, the modulation of the activity of MOFs by precisely tuning the coordination of metal nodes is still a significant challenge. Inspired by metalloenzymes with well-defined coordination structures, a series of MOFs containing halogen-coordinated copper nodes (Cu-X MOFs, X = Cl, Br, I) are employed to elucidate their structure-activity relationship.

N - Methyladenosine defines a new checkpoint in γδ T cell development.

T cells, which are derived from hematopoietic stem cells (HSCs), are the most important components of adaptive immune system. Based on the expression of αβ and γδ receptors, T cells are mainly divided into αβ and γδ T cells. In the thymus, they share common progenitor cells, while undergoing a series of well-characterized and different developmental processes.

Corrigendum: Phytochromes A and B mediate light stabilization of BIN2 to regulate brassinosteroid signaling and photomorphogenesis in .

[This corrects the article DOI: 10.3389/fpls.2022.

Levocarnitine regulates the growth of angiotensin II-induced myocardial fibrosis cells via TIMP-1.

This study aimed to explore the effects of tissue inhibitor of metalloproteinases-1 (TIMP-1) on levocarnitine (LC)-mediated regulation of angiotensin II (AngII)-induced myocardial fibrosis (MF) and its underlying mechanisms. H9C2 cells were treated with AngII for 24 h to induce fibrosis. The cells were then treated with LC or transfected with TIMP-1-OE plasmid/si‑TIMP-1.

Screening and Analysis of Serum Protein Biomarkers Infected by Coronavirus Disease 2019 (COVID-19).

Coronavirus disease 2019 (COVID-19) has spread widely around the world, and in-depth research on COVID-19 is necessary for biomarkers and target drug discovery. This analysis collected serum from six COVID-19-infected patients and six healthy people. The protein changes in the infected and healthy control serum samples were evaluated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography (HPLC).

Detection by environmental surveillance and genomic characterization of H5N8 highly pathogenic avian influenza virus from a poultry meat market in Beijing, China, 2021-22.

Since 2010 the year when it was first reported in domestic ducks in China, highly pathogenic avian influenza (HPAI) H5N8 has caused several outbreaks in different countries. The first outbreak wave was documented in South Korea and Japan in 2014 and the second wave was reported in Asian and European countries in 2016. More importantly, zoonotic infection was first reported in poultry workers in Russia in 2021.

An Updated Review on SARS-CoV-2 Infection in Animals.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has lasted for two years and caused millions of infections and deaths in humans. Although the origin of SARS-CoV-2 infection in humans remains unknown, infection in animals has been frequently reported in varieties of animals all over the world. Both experimental and natural infections of SARS-CoV-2 in different animal species provide useful information on viral host range and pathogenicity.

Using Wool Keratin Derived Metallo-Nanozymes as a Robust Antioxidant Catalyst to Scavenge Reactive Oxygen Species Generated by Smoking.

Self-assembled nanostructures based on biomolecules (e.g., proteins and amino acids) and metal ions have promising applications in mimicking the nanostructure, properties, and functions of natural enzymes.

Phytochromes A and B Mediate Light Stabilization of BIN2 to Regulate Brassinosteroid Signaling and Photomorphogenesis in .

Phytochromes A and B (phyA and phyB) are the far-red and red lights photoreceptors mediating many light responses in . Brassinosteroid (BR) is a pivotal phytohormone regulating a variety of plant developmental processes including photomorphogenesis. It is known that phyB interacts with BES1 to inhibit its DNA-binding activity and repress BR signaling.

Changes in Oxygen Availability during Glucose-Limited Chemostat Cultivations of Lead to Rapid Metabolite, Flux and Productivity Responses.

Bioreactor scale-up from the laboratory scale to the industrial scale has always been a pivotal step in bioprocess development. However, the transition of a bioeconomy from innovation to commercialization is often hampered by performance loss in titer, rate and yield. These are often ascribed to temporal variations of substrate and dissolved oxygen (for instance) in the environment, experienced by microorganisms at the industrial scale.

Hybrid FSK-PSK Waveform Optimization for Radar Based on Alternating Direction Method of Multiplier (ADMM).

In this paper, a new radar signal modulated with a hybrid of the frequency shift keying (FSK) and the phase shift keying (PSK) signal-i.e., the FSK-PSK signal-is studied.