Development and validation of a multi-platform LAMP system for rapid HAdV-3 and HAdV-7 detection.

Purpose: We developed a loop-mediated isothermal amplification (LAMP) multi-platform detection system that overcomes the limitations of traditional polymerase chain reaction (PCR) in timeliness and equipment dependency, establishing a rapid, accurate diagnostic framework for Human adenovirus types 3 and 7 (HAdV-3 and HAdV-7) across resource-varied clinical settings.

Methods: LAMP primers were designed based on the conserved regions of the Hexon genes of HAdV-3 and HAdV-7. The calcein, immunochromatography (IC), and fluorescence probe methods were used.

diagnosis based on SERS-LFIA: research hotspots, increase sensitivities, combined detection, multimodal detection and related patents.

In recent years, the SERS-LFIA platform has gained significant traction in diagnostics. However, a comprehensive review of its advancements and applications is still lacking. This review first employing a bibliometric approach to analyze research trends.

Molecular characteristics of macrolide-resistant Mycoplasma pneumoniae in children with community-acquired pneumonia in Urumqi, Xinjiang, China in autumn, winter, and spring 2023-2024.

Background: Following the COVID-19 pandemic, the resurgence of Mycoplasma pneumoniae (MP) infections among children in China has raised urgent concerns regarding antimicrobial drug resistance. We reveal the molecular characteristics of macrolide-resistant MP (MRMP) in children with community-acquired pneumonia (CAP) in autumn, winter, and spring 2023-2024 in Urumqi, Xinjiang, China.

Methods: Throat samples were collected from 1446 children hospitalized for CAP between October 2023 and April 2024.

Correlation analysis of key genes and immune infiltration in visceral adipose tissue and subcutaneous adipose tissue of patients with type 2 diabetes in women.

Immune cell infiltration into adipose tissue (AT) is a key factor in type 2 diabetes (T2DM). However, research on the impact of fat distribution on immune cells and immune responses in women is still lacking. This study used enrichment, protein-protein interaction network, immune cell infiltration, and correlation analysis to compare the similarities and differences between the transcriptome data of visceral AT (VAT) and subcutprotein-proteinaneous AT (SAT) obtained from the omprehensive database of gene expression in women with non-T2DM and T2DM.