GJB2 promotes ovarian cancer progression and cisplatin resistance by upregulating TNC expression.

Cisplatin resistance continues to be a major obstacle in the treatment of ovarian cancer (OC). Gap junction protein β-2 (GJB2), a key member of the connexin family, is well-known for its association with hereditary deafness. However, its role in ovarian cancer chemotherapy resistance remains unexplored.

Fungal Necromass Carbon Dominates Global Soil Organic Carbon Storage.

Soil organic carbon (C) is derived primarily from plant and microbial necromass; however, the global distribution and contribution of different necromass inputs to soil C storage remain unclear. We conducted a global meta-analysis encompassing 2410 observations from 249 microbial necromass studies and 786 observations from 72 plant residue studies. The results showed that the content of microbial-derived C (10.

High-Fidelity DNA Polymerase for DNA-Based Digital Information Storage.

DNA is increasingly recognized for its superior data storage density, favorable stability, and low energy requirements, positioning it as a potential alternative for future digital information storage systems. However, the replication and transfer of information within DNA is prone to errors, primarily due to the inaccuracy during DNA synthesis. Herein, 9°N DNA polymerase is explored from Thermococcus sp.

Global Synthesis of Fertilisation-Induced Changes in the Microbial Entombing Effect.

The microbial entombing effect refers to the accumulation and stabilisation of microbially derived carbon (C) in soils following the sustained production of microbial biomass and necromass. Fertilisation practices modify soil microbial activity and C cycling and consequently influence the microbial entombing effect, which has implications for global C sequestration. We conducted a global meta-analysis to evaluate the impact of fertilisation practices on the microbial entombing effect, focusing on microbial necromass C (MNC), microbial biomass C (MBC) and the microbial necromass accumulation coefficient (NAC = MNC/MBC) across 319, 1665 and 199 paired datasets, respectively.

Research on Creep Deformation of Dissimilar FSWed T-Joints Under Different Ultrasonic Vibration Modes: Experiment, Constitutive Model, and Simulation Verification.

This article presents experimental and numerical studies on the creep deformation of 7055-T6 Al and 2197-T8 Al-Li T-joints. Firstly, the optimal process parameters for creep tensile tests (CATs) are determined to be 155 °C, 130 MPa, and 8 h. Based on this, different modes of ultrasonic vibration are introduced.

Indoor Evaluation of a Temperature-Controlled Gel Intelligent Diversion System.

The Bohai SZ36-1 oilfield, the largest offshore oilfield in China, features a high-porosity, high-permeability reservoir with significant heterogeneity and permeability variations. After extended water injection, the reservoir's pore structure evolved, increasing heterogeneity and reducing the effectiveness of traditional production methods. To address these issues, this study introduces an intelligent diversion and balanced unblocking technology, using a temperature-controlled diversion system to block dominant flow channels and ensure even distribution of treatment fluids while maintaining reservoir integrity.

A framework for resilience assessment of transportation networks exposed to geohazard threats.

The increased frequency of rainfall-triggered geohazards has led to more disruptions of transportation networks in recent years. This study proposes an integrated framework for resilience assessment of transportation networks, where the highway disruption scenarios are simulated using a traffic model and a developed geohazard threat model based on real-world datasets. In the framework, we provide a resilience-oriented indicator that integrates traffic flow and geohazard threat, upon which to identify critical elements of the highway network and the geohazard-prone sites.

Development of sawtooth waveform beam buncher for medical cyclotron accelerator.

A bunching system utilizing a heterodyne combiner is designed to generate a sawtooth waveform with three harmonics to increase the beam current intensity of medical cyclotron accelerators. The design incorporates a low-pass filter, helical bandpass filters, and a broadband matching network, producing a sawtooth waveform exceeding 1200 V, which meets the requirements for efficient bunching. This buncher substitutes the design and manufacturing requirements for high linearity broadband power amplifiers with three narrowband single-frequency power amplifiers for amplification.

Functional characterization of Ganoderma lucidum polysaccharide and the mechanism of inducing tomato resistance to Tomato yellow leaf curl virus.

Using functional polysaccharides as elicitors to induce plant disease resistance has emerged as an effective plant protection strategy. In this study, we found that crude polysaccharides derived from by-products of Ganoderma lucidum can effectively prevent and control the Tomato Yellow Leaf Curl Virus (TYLCV). As a result, we isolated and purified a homogeneous polysaccharide known as glucan-like peptide (GLP1), from crude polysaccharide of G.

Engineering thermostability of industrial enzymes for enhanced application performance.

Thermostability is a key factor for the industrial application of enzymes. This review categorizes enzymes by their applications and discusses the importance of engineering thermostability for practical use. It summarizes fundamental theories and recent advancements in enzyme thermostability modification, including directed evolution, semi-rational design, and rational design.

Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling.

transglutaminase (smTG) can be used for site-specific labeling of proteins with chemical groups. Here, we explored the use of modified smTG for the biosynthesis of nanobody-fluorophore conjugates (NFC). smTG catalyzes the conjugation of acyl donors containing glutamine with lysine-containing acceptors, which can lead to non-specific cross-linking.

Re-designing a yield-improving strategy of pear orchards in the Yangtze River Basin, China.

Background: Pear yield is a primary source of income for smallholder farmers in China, yet significant yield disparities exist among different smallholders. Systematic analyses of limiting factors and the feasibility of solutions at the smallholder level are limited. This study employs a novel DEED (Describe, Explain, Explore, and Design) research cycle centered on smallholders to formulate yield-improving strategies.

Molecular dynamics simulation of hybrid structure and mechanical properties of DLC/Ni-DLC thin films.

To improve the mechanical properties of the rolling body surface of wind power bearings, extend its service life. In this study, a large-scale molecular/atomic parallel processor LAMMPS was introduced, and then the process of magnetron sputtering technology in the preparation of DLC/Ni-DLC thin films on the 42CrMo substrate material was simulated. The effects of deposition parameters such as sputtering temperature, sputtering voltage, deposition air pressure, and Ni doping on the residual stress, film base bonding, and organizational structure of the thin films were investigated.

Successful treatment of resistant hypertension and severe complications in a 63-year-old man with primary aldosteronism without adrenalectomy: A case report.

Primary aldosteronism (PA), often due to aldosteronoma, commonly causes secondary hypertension and typically requires surgery. We present a case of an elderly man with longstanding hypertension, complicated by cerebral hemorrhage and myocardial infarction. Enhanced CT imaging identified a right-sided aldosteronoma and left adrenal gland fullness.

AI-Driven Sensing Technology: Review.

Machine learning and deep learning technologies are rapidly advancing the capabilities of sensing technologies, bringing about significant improvements in accuracy, sensitivity, and adaptability. These advancements are making a notable impact across a broad spectrum of fields, including industrial automation, robotics, biomedical engineering, and civil infrastructure monitoring. The core of this transformative shift lies in the integration of artificial intelligence (AI) with sensor technology, focusing on the development of efficient algorithms that drive both device performance enhancements and novel applications in various biomedical and engineering fields.

TP53 mutation promotes breast cancer cell proliferation through CORO1A-P38 MAPK pathway regulation.

Breast cancer is the most commonly diagnosed cancer in women. Among all types, triple-negative breast cancer is particularly challenging to cure because of its high recurrence rates and invasive and metastatic capacity. Although numerous studies have explored the role of TP53 mutations in cancer, there is a dearth of research regarding the correlation between TP53 mutations and breast cancer cell proliferation.

Unblocking Process of Complex Sulfur-Iron Scale Blockage in Sulfur-Bearing Gas Wells and Its Mechanism.

In this paper, through the establishment of a blockage analysis process, multistage weight loss method, and FT-IR, GC-MS, SEM, EDS, XRD and other characterization test means, it is clear that the scale sample is mainly organic material covered with complex and diverse sulfur and iron compounds, and the mechanism of sulfur-containing gas well blockage is revealed by tracing into the well material, formation minerals, acid gas corrosion, and other substances. Through the selection of chelating agent, cleaning agent, and acid concentration, as well as the optimization of different process parameters, the "dispersion-chelation-dissolution" composite unblocking technology is proposed, and a set of unblocking formula systems for complex sulfur and iron scale is clarified. Finally, XRD and SEM characterization tests of the scale samples before and after dissolution were conducted to study the declogging mechanism and confirm the acid-insoluble nature of FeS and its stability in a weak acidic environment.

A Deep Learning-Based Unbalanced Force Identification of the Hypergravity Centrifuge.

Accurate and quantitative identification of unbalanced force during operation is of utmost importance to reduce the impact of unbalanced force on a hypergravity centrifuge, guarantee the safe operation of a unit, and improve the accuracy of a hypergravity model test. Therefore, this paper proposes a deep learning-based unbalanced force identification model, then establishes a feature fusion framework incorporating the Residual Network (ResNet) with meaningful handcrafted features in this model, followed by loss function optimization for the imbalanced dataset. Finally, after an artificially added, unbalanced mass was used to build a shaft oscillation dataset based on the ZJU-400 hypergravity centrifuge, we used this dataset to train the unbalanced force identification model.

Effect of planting and mowing cover crops as livestock feed on soil quality and pear production.

Introduction: The increasing demand for animal-products has led to an increasing demand for livestock feed. Using cover crop as green manure in orchards is an effective measure to improve fruit yield and quality. However, the effect of mowing cover forage crops as livestock feed on soil quality and crop production is unclear.

Sustainable strategies related to soil fertility, economic benefit, and environmental impact on pear orchards at the farmer scale in the Yangtze River Basin, China.

Pears are an important income source in China, and unreasonable management practices have had a negative impact on the sustainability of pear orchards. However, multi-objective synergistic strategies are unclear on a farmer scale. In this study, we quantified indicators of soil fertility (soil organic matter (SOM)), environmental impact (global warming potentials (GWP)), and economic benefit (ratio of benefit and cost (BCR)) and analysed the synergetic strategies based on survey data from 230 smallholders in the Yangtze River Basin (Shanghai City, Chongqing City, Zhejiang province, and Jiangxi province).