Transcriptomic and Proteomic Insights into 6PPD/6PPD-Q Induced Oxidative Stress in Black-Spotted Frogs.

N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) and its oxidation product 6PPD-quinone (6PPD-Q) can have lethal effects on aquatic organisms, interfering with gene expression and protein content in aquatic animals. In this study, we performed proteomics and transcriptomics analyses on the livers of black-spotted frogs exposed to 6PPD and 6PPD-Q. The results showed that 6PPD and 6PPD-Q can cause oxidative stress in the liver, significantly reducing catalase (CAT) and glutathione peroxidase (GSH-Px) levels, with 6PPD-Q having a more significant toxic effect.

Recent Advances in Heavy Metal Stabilization and Resource Recovery from Municipal Solid Waste Incineration Fly Ash.

Municipal solid waste incineration fly ash (MSWI FA) is recognized as a hazardous solid waste due to its enrichment in toxic heavy metals and high leaching potential. This review systematically summarizes the current understanding of heavy metal occurrence in MSWI FA and associated environmental risks. Solidification and stabilization methods, such as cement-based curing and chemical immobilization, are widely applied due to their cost-effectiveness and operability, though their long-term stability and recovery potential remain limited.

Blood pressure is elevated in the absence of resistance artery dysfunction in a mouse model of diet-induced obesity.

Introduction: Hypertension and impaired tissue perfusion are frequent comorbidities in obesity. Since resistance arteries are the primary regulators of peripheral resistance and hence, systemic blood pressure and local blood flow control, we hypothesized that resistance arteries isolated from obese mice would display augmented myogenic reactivity and altered vasomotor responses, compared to non-obese controls.

Methods: Eight-week-old C57BL/6J mice were fed either a high-fat diet (60% calories from fat; HFD) or a matched control diet for 16 weeks.

Toxicological effects of sulfamethoxazole (SMX) and tire micro-particles (TMPs), alone or in combination, on the hepatopancreas of Litopenaeus vannamei.

As the metabolic center of crustacean invertebrates, hepatopancreas plays a crucial role in metabolic detoxification and nutrient storage. Although sulfamethoxazole (SMX) and tire micro-particles (TMPs) are emerging marine pollutants, their combined hepatotoxic effects on lipid metabolism in crustaceans remain poorly understood. In this study, Litopenaeus vannamei were exposed to SMX and TMPs for 14 days.

Evaluation of Fly Ash Composition from Municipal Solid Waste Incinerators: The Role of the Incinerator Type and Flue Gas Deacidification Process.

The resource utilization potential and environmental impact of fly ash from municipal solid waste incinerators (MSWIs) have attracted wide attention. In this study, four MSWIs in Hangzhou, Zhejiang Province were selected to systematically evaluate the effects of different incinerator types and flue gas deacidification processes on fly ash's oxide and heavy metal components and their temporal changes as well as conduct risk assessment. The results showed that the contents of MgO, AlO, SiO, and FeO in the grate furnace fly ash were significantly lower than those in the fluidized bed fly ash, but the compressive strength of its fly ash was high.

Sustained exposure to triclocarban and triclosan at environmental relevant concentration disrupts gut-liver axis in the black-spotted frog.

Triclocarban (TCC) and triclosan (TCS) are broad-spectrum biocides that are extensively utilized frequently contaminate water bodies and have high environmental persistence. However, the safety of TCC and TCS for wild organisms remains largely unknown. Here, we evaluated the potential health risks for black-spotted frogs (Pelophylax nigromaculatus) when exposed to TCC and TCS at concentrations of 1, 10, and 100 μg/L for 21 consecutive days.

Chronic exposure to environmental concentrations of enrofloxacin impairs neutrophil function in zebrafish via HPT axis disruption and hematopoietic dysregulation.

The widespread detection of fluoroquinolone antibiotics like enrofloxacin (ENR) in aquatic ecosystems raises significant concerns about ecological and immunological impacts. While acute ENR toxicity is documented, the immunotoxic effects of chronic, environmentally relevant exposure remain poorly understood. This study investigates the mechanisms by which long-term ENR exposure (28 days at 10-100 μg/L,concentrations reflecting common pollution levels in freshwater systems near aquaculture facilities and extreme contamination scenarios due to wastewater treatment failure) compromises zebrafish immunity, focusing on neutrophil dysfunction.

Triclocarban and Triclosan disturb Lipometabolism via PPAR in black-spotted frogs: In vivo and molecular dynamics simulation studies.

Triclocarban (TCC) and triclosan (TCS) are broad-spectrum antimicrobial agents that are widely distributed in aquatic environments. They pose significant risks to aquatic organisms, but their potential impacts on animal health remain poorly understood. In this study, black-spotted frogs (Pelophylax nigromaculatus) were exposed to TCC or TCS at concentrations of 1, 10, or 100 μg/L for 21 days to investigate the effects of these agents on hepatic lipid metabolism.

Photo-Enhanced Peroxymonosulfate Activation via Well-Dispersed Cobalt Nanoparticles Encapsulated on Carbon Nitride for 2,8-Dichlorodibenzo-p-dioxin Removal.

The removal of polychlorinated dibenzo-p-dioxins (PCDDs) via advanced oxidation processes (AOPs) poses a significant challenge due to their high toxicity and chemical stability. In this study, a series of well-dispersed cobalt nanoparticles supported on carbon nitrides (xCoCNs) was synthesized to activate peroxymonosulfate (PMS) for 2,8-dichlorodibenzo-p-dioxin (2,8-DCDD) degradation under visible light. The catalysts prepared were characterized using SEM, XPS, photoluminescence (PL), and UV-Vis diffuse reflectance spectroscopy (UV-Vis DRS).

3,3',5,5'-Tetrabromobiphenyl (BB-80) and Its Hydroxylation Product (OH-BB-80) Mediate Immunotoxicity and Inhibit Embryonic Development in Zebrafish () via the TLR4/NF-κB Signaling Pathway.

Polybrominated biphenyls (PBBs) are metabolically transformed into monohydroxylated PBBs (OH-PBBs) in the environment and living organisms. Although OH-PBBs pose a significant health threat to organisms, little is known about the immunotoxicity of OH-PBBs. Therefore, the objectives of this study were to validate BB-80 and OH-BB-80 induced immunotoxicity and to explore the associated pathway mechanisms.

Fluoxetine impairs gamete function and fertilization success in Tegillarca granosa: environmental risks of antidepressant contamination.

In recent years, the antidepressant fluoxetine (FLX) has been increasingly detected in global environments, emerging as a contaminant with significant toxic effects. However, its impact on the fertilization processes of broadcast-spawning species remains unclear. This study focuses on Tegillarca granosa, a broadcast-spawning bivalve, to evaluate the effects of fluoxetine on gametes and fertilization success.

Endothelial dysfunction drives atherosclerotic plaque macrophage-dependent abdominal aortic aneurysm formation.

Currently there is no effective pharmacotherapy to prevent the growth and rupture of abdominal aortic aneurysms. Using a mouse model that combines cigarette smoke exposure and hypercholesterolemia, we demonstrated that cigarette smoke exacerbated atherosclerosis, leading to elastin fragmentation, aneurysm formation, rupture and death. Arterial injury was driven by macrophages that accumulated within atherosclerotic plaques and exhibited tissue-degrading proteolytic activity in vivo (a process dependent on the endothelial cell-derived macrophage growth factor CSF-1).

Decoupling of diversity and network complexity of bacterial communities during water quality deterioration.

Numerous studies have examined the impact of water quality degradation on bacterial community structure, yet insights into its effects on the bacterial ecological networks remain scarce. In this study, we investigated the diversity, composition, assembly patterns, ecological networks, and environmental determinants of bacterial communities across 20 ponds to understand the impact of water quality degradation. Our findings revealed that water quality degradation significantly reduces the α-diversity of bacterial communities in water samples, while sediment samples remain unaffected.

Multigenerational toxic effects in Daphnia pulex are induced by environmental concentrations of tire wear particle leachate.

Microplastic pollution has emerged as the second most significant scientific issue in environmental science and ecology. Similarly, the biological effects of tire wear particles (TWPs) have garnered considerable research attention; however, studies on chronic TWP leachate toxicity at environmentally relevant concentrations remain sparse. Here, we investigated the effects of TWP leachate at environmentally relevant concentrations (0.

Cobalt-Doped Carbon Nitride for Efficient Removal of via Peroxymonosulfate Activation.

Heterogeneous persulfate activation is an advanced technology for treating harmful algae in drinking water sources, while it remains a significant hurdle in the efficient management of cyanobacterial blooms. In this study, super-dispersed cobalt-doped carbon nitride (2CoCN) was prepared to activate peroxymonosulfate (PMS) for simultaneous inhibition and microcystin (MC-LR) degradation. When the initial PMS and 2CoCN concentrations were 0.

Environmental concentrations of 6PPD and 6PPD-quinone induce hepatic lipid metabolism disorders in male black-spotted frogs.

Aquatic environments are generally contaminated with N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) and its oxidation product 6PPD-quinone (6PPD-Q). Recently, 6PPD-Q was found lethally toxic to some specific species, especially salmonid silverfish. This study investigated male black-spotted frogs (Pelophylax nigromaculatus) exposed to 6PPD and 6PPD-Q with different environmental concentrations (0, 1, and 10 μg/L) for 21 days, after which biochemical, metabolomic, gene expression analyses, and molecular docking were conducted.

Simultaneous determination of 18 steroids in the hypothalamic pituitary gonadal axis based on UPLC-MS/MS with multimode ionization.

To objectively quantify changes in steroid hormones in organisms caused by adverse environmental loads, we developed a simple and sensitive UPLC-MS/MS (ultra-performance liquid chromatography triple quadrupole mass spectrometry) method for the simultaneous determination of 18 steroid hormones on the HPG axis. This analytical method was based on liquid extraction and a multimode electrospray and atmospheric pressure chemical ionization (ESCi) source, which was optimized by mass spectrometry, liquid phase and pretreatment for the quantification of cholesterol (CH), aldosterone (A), cortisone (E), hydrocortisone (F), 21-deoxycortisol (21-DF), corticosterone (B), 11-deoxycortisol (11-DF), androstenedione (A), estradiol (E), estrone (E), 2-methoxyestradiol (2-MeE), 21-hydroxyprogesterone (21-OHP), 17-α hydroxyprogesterone (17α-OHP), testosterone (T), dehydroepiandrosterone (DHEA), progesterone (P), dihydrotestosterone (DHT), and pregnenolone (P). The method exhibits linearity in the analyte-concentration range 0.

Effects of environmental concentrations of 6PPD and its quinone metabolite on the growth and reproduction of freshwater cladoceran.

The widespread occurrence and accumulation of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) and its quinone metabolite, 6PPD quinone (6PPD-Q), have been globally recognized as a critical environmental issue. However, knowledge on the adverse effects of 6PPD and 6PPD-Q on freshwater invertebrates is limited. This study investigated the effects of 6PPD and its oxidative byproduct, 6PPD-Q, on the growth and reproduction of Daphnia pulex.

Metformin as an Emerging Pollutant in the Aquatic Environment: Occurrence, Analysis, and Toxicity.

The use of human and veterinary drugs has led to the accumulation of pharmaceuticals in various aquatic environments at progressively increasing levels, exhibiting strong ecological risks. Metformin is widely used as a first-line prescription drug for the treatment of type 2 diabetes mellitus as well as a livestock drug. Unlike other drugs, metformin is not metabolized in the body, and almost all of its intake is excreted and released into the aquatic environment via urine and feces, causing adverse effects on aquatic ecosystems.

Metabolomics reveals the lipid metabolism disorder in Pelophylax nigromaculatus exposed to environmentally relevant levels of microcystin-LR.

Cyanobacterial blooms have emerged as a significant environmental issue worldwide in recent decades. However, the toxic effects of microcystin-LR (MC-LR) on aquatic organisms, such as frogs, have remained poorly understood. In this study, frogs (Pelophylax nigromaculatus) were exposed to environmentally relevant concentrations of MC-LR (0, 1, and 10 μg/L) for 21 days.

Cytochrome P450 enzymes in the black-spotted frog (s): molecular characterization and upregulation of expression by sulfamethoxazole.

Cytochrome P450 (CYP) enzymes are crucial for the detoxification of xenobiotics, cellular metabolism, and homeostasis. This study investigated the molecular characterization of CYP enzymes in the black-spotted frog, , and examined the regulation of CYP expression in response to chronic exposure to the antibiotic sulfamethoxazole (SMX) at various environmental concentrations (0, 1, 10, and 100 μg/L). The full-length cDNA of Pn-CYP26B1 was identified.

Tetrabromobisphenol A reduces male rats reproductive organ coefficients and disrupting sexual hormone by causing oxidative stress.

Tetrabromobisphenol A (TBBPA) has become a topic of public attention due to its pervasive detection in the environment and organisms in recent decades. However, limited information is available regarding the toxicity of TBBPA on reproductive ability of male mammals. Herein, the reproductive toxicity of TBBPA was investigated in male rats to fill the knowledge gap.

Microcystin-LR Induces Estrogenic Effects at Environmentally Relevant Concentration in Black-Spotted Pond Frogs (): , , , and Investigations.

Harmful cyanobacterial blooms are frequent and intense worldwide, creating hazards for aquatic biodiversity. The potential estrogen-like effect of Microcystin-LR (MC-LR) is a growing concern. In this study, we assessed the estrogenic potency of MC-LR in black-spotted frogs through combined field and laboratory approaches.

Paternal exposures to endocrine-disrupting chemicals induce intergenerational epigenetic influences on offspring: A review.

Endocrine-disrupting chemicals (EDCs) are ubiquitous in ecological environments and have become a great issue of public health concern since the 1990 s. There is a deep scientific understanding of the toxicity of EDCs. However, recent studies have found that the abnormal physiological functions of the parents caused by EDCs could be transmitted to their unexposed offspring, leading to intergenerational toxicity.

Defining Transcriptomic Heterogeneity between Left and Right Ventricle-Derived Cardiac Fibroblasts.

Cardiac fibrosis is a key aspect of heart failure, leading to reduced ventricular compliance and impaired electrical conduction in the myocardium. Various pathophysiologic conditions can lead to fibrosis in the left ventricle (LV) and/or right ventricle (RV). Despite growing evidence to support the transcriptomic heterogeneity of cardiac fibroblasts (CFs) in healthy and diseased states, there have been no direct comparisons of CFs in the LV and RV.