Src Reduces Neutrophil Extracellular Traps Generation and Resolves Acute Organ Damage.

Neutrophil extracellular traps (NETs) are key factors mediating acute inflammatory injury. However, the underlying mechanisms and potential therapeutic targets remain unclear. Previous results suggest Src may be involved in regulating the NETs formation.

Muscle strength and mass as predictors of pancreatic cancer: insights from the UK biobank.

Background: While sarcopenia is associated with adverse pancreatic cancer (PC) outcomes, prospective evidence linking muscle health to PC risk remains limited. This study aimed to prospectively investigate the associations of grip strength and muscle mass with incident PC risk in the UK Biobank cohort.

Methods: Among 363,693 UK Biobank participants, grip strength (kg) and bioelectrical impedance analysis (BIA)-derived muscle mass (weight-normalized) were measured.

Deletion of gasdermin D promotes granulocytic myeloid-derived suppressor cell differentiation by decreased release of mitochondrial DNA to promote tumor escape.

Gasdermin D (GSDMD), an effector molecule of cell pyroptosis, is known to be activated in various cells during inflammation. However, the patterns of GSDMD activation in immune regulatory cells such as myeloid-derived suppressor cells (MDSCs) remain unclear. In this study, we found that neutrophils in colorectal cancer (CRC) tissues exhibited reduced GSDMD transcription, as evidenced by a single-cell RNA sequencing result.

Identification and validation of the pivotal role of MANF gene in pancreatic β-cell aging using bioinformatics.

Pancreatic β-cells are essential for maintaining endocrine function, and their age-related decline is strongly associated with insulin resistance and an increased risk of developing diabetes. By integrating cross-species bioinformatics analyses (single-cell RNA-seq data from young and aged cynomolgus macaques and microarray data from young and old mouse pancreatic β-cells), we identified mesencephalic astrocyte-derived neurotrophic factor (MANF), an endoplasmic reticulum (ER) stress-related gene, as a key regulator of β-cell aging. Using a d-galactose (D-gal)-induced aging mouse model (400 mg/kg/day for 10 weeks) and HO (300 μM)-treated MIN6 cells, we demonstrated that MANF expression was downregulated in the aging models, which also exhibited elevated levels of cyclin-dependent kinase inhibitor P21, insulin resistance, impaired glucose tolerance, and decreased insulin secretion.

XML Attenuates Ox-LDL-Induced Endothelial Progenitor Cell Senescence via Gria2 and cAMP Pathways.

In-stent restenosis (ISR) following percutaneous coronary intervention (PCI) is a critical clinical issue, often arising from endothelial injury and impaired repair mechanisms. Endothelial progenitor cells (EPCs), derived from bone marrow, play a key role in vascular health, but their function diminishes with aging and exposure to oxidised low-density lipoprotein (ox-LDL). This study investigates the potential of Xin-Mai-Long (XML), a traditional Chinese medicine used for chronic congestive heart failure, to delay EPC senescence and dysfunction induced by ox-LDL.

High-dose IL-37 promotes inflammatory activity of macrophage via Preferential ligation of the IL-18Rα.

Interleukin-37 (IL-37) exists in various human tissues and organs and exhibits extensive anti-inflammatory activity. Recombinant IL-37 ameliorates the insulin resistance and inflammation associated with obesity and type 2 diabetes (T2DM). However, the specific role and underlying mechanisms of different doses of IL-37 in macrophage inflammatory response in T2DM remain to be discovered.

Uric acid and related disorders in the risk of pancreatic diseases: A UK Biobank prospective study.

Background: Pancreatic diseases, such as acute pancreatitis (AP), chronic pancreatitis (CP), and pancreatic cancer (PC) present major clinical challenges with significant health impacts. Serum uric acid (SUA), resulting from purine metabolism, is a significant biomarker reflecting metabolic health. Defined as SUA >7.

The association between pancreatic diseases and pancreatic fat content: a cross-sectional study from the UK Biobank.

Objective: Many researches have demonstrated an association between intra-pancreatic fat deposition (IPFD) and several pancreatic pathological conditions, including pancreatitis, pancreatic cancer, and type 2 diabetes mellitus (T2DM). The aim of this study is to investigate the influence of pancreatic diseases on the accumulation of pancreatic fat, to further explore which kind of pancreatic disease is significant, and to find out the possible mediating factors.

Methods: A cross-sectional study based on the UK Biobank (UKB) data categorized participants by pancreatic disease status and collated relevant information.

Identification of Grb2 protein as a potential mediator of macrophage activation in acute pancreatitis based on bioinformatics and experimental verification.

Introduction: Macrophage activation is closely associated with Acute pancreatitis (AP). We screened and found that Growth factor receptor bound protein 2 (Grb2) is highly expressed in macrophages during AP. However, the relationship between Grb2 and AP is still poorly understood.

A systematic review of the epidemiology and risk factors for severity and recurrence of hypertriglyceridemia-induced acute pancreatitis.

This systematic review aims to comprehensively assess the epidemiology and identify risk factors associated with the severity and recurrence of hypertriglyceridemia-induced acute pancreatitis (HTG-AP). A search of PubMed, Web of Science, and Cochrane databases was conducted to identify all relevant randomized controlled trials (RCTs), prospective, or retrospective cohort studies on HTG-AP. Data related to epidemiology and risk factors for severity and recurrence of HTG-AP were extracted and analyzed.

IL-37 Protects Against Ventilator-Induced Lung Injury by Inhibiting NLRP3 Activation.

Mechanical ventilation is an effective strategy for managing acute respiratory distress syndrome (ARDS), but it can also exacerbate lung injury, leading to ventilator-induced lung injury (VILI). To investigate the protective role of interleukin-37 (IL-37) in the pathogenesis of VILI, we used two approaches, human IL-37 transgenic (IL37tg) mice and administration of recombinant human IL-37 (rIL37) in wild-type (WT) mice subjected to mechanical ventilation. Lung histopathology was evaluated, inflammatory cytokine levels (IL-1β, IL-6, TNF-α) were measured, and inflammasome activation was assessed by analyzing NLRP3 and Caspase-1 expression.

Angiotensinogen inhibition concurrently mitigates alcohol-associated hepatic and muscle injury.

Aims: The organ communication mechanisms driven by alcohol-associated liver disease (ALD) remain inadequately understood. This study explores the endocrine roles of the hepatokine angiotensinogen (AGT) and the renin-angiotensin system (RAS) in ALD.

Methods And Results: Hepatokine screening tests revealed that chronic-binge ethanol consumption upregulates hepatic AGT production, triggering downstream RAS activation.

Inhibition of CD53 Reduces the Formation of ROS-Induced Neutrophil Extracellular Traps and Protects Against Inflammatory Injury in Acute Pancreatitis.

Background: The tetraspanin CD53 transmembrane protein is vital in immune cells like B cells and T cells, playing a crucial role in various inflammatory conditions. However, its involvement in neutrophils regarding inflammation remains uncertain. This study aims to examine the impact of CD53 on neutrophil extracellular traps (NETs) formation.

Pentraxin3 exacerbates acute pancreatitis injury by inhibiting oxidative phosphorylation pathway.

Local pancreatic necrosis and systemic inflammatory response caused by acute pancreatitis (AP) are closely related to the disease prognosis and severity. This study aimed to explore whether pentraxin 3 (PTX3) regulates AP pancreatic necrosis and reveals the underlying mechanism. By using AP time gradient transcriptomics, proteomics and liquid phase chip analysis, we found a close association between PTX3 and AP.

Clinical Outcomes in a Multicenter Cohort Involving 919 Patients With Hypertriglyceridemia-Associated Acute Pancreatitis.

Introduction: Hypertriglyceridemia-associated acute pancreatitis (HTG-AP) is one of the most common etiologies of acute pancreatitis (AP) worldwide. Compared with other etiologies, patients with HTG-AP may develop more severe AP, but previous studies yielded controversial conclusion due to the lack of adequate adjustment for the confounders. Therefore, the aim of this study was to examine the possibility and risk factors of developing severe AP in HTG-AP.

Differentiating Pulmonary Nodule Malignancy Using Exhaled Volatile Organic Compounds: A Prospective Observational Study.

Background: Advances in imaging technology have enhanced the detection of pulmonary nodules. However, determining malignancy often requires invasive procedures or repeated radiation exposure, underscoring the need for safer, noninvasive diagnostic alternatives. Analyzing exhaled volatile organic compounds (VOCs) shows promise, yet its effectiveness in assessing the malignancy of pulmonary nodules remains underexplored.

Rhaponticin Alleviates Collagen-induced Arthritis by Inhibiting NLRP3/GSDMD-mediated Neutrophil Extracellular Traps.

Neutrophil extracellular traps (NETs) play an important role in the inflammatory response and progressive joint destruction in rheumatoid arthritis (RA). Rhaponticin (Rha) is a stilbene glycoside compound with antioxidant and anti-inflammatory effects. This study aimed to investigate the therapeutic potential of Rha in RA, with a specific focus on its effects on NETs and on the underlying mechanisms of Rha.

LASP1 inhibits the formation of NETs and alleviates acute pancreatitis by stabilizing F-actin polymerization in neutrophils.

Background: Neutrophil extracellular traps (NETs) play a significant role in the development of acute pancreatitis (AP). The actin-binding protein LASP1 regulates proteins associated with the cytoskeleton, yet its precise involvement in NETs and AP remains to be elucidated.

Methods: To investigate the role of LASP1 in NETs and AP, several bioinformatics methods, such as weighted gene co-expression network analysis (WGCNA), differential analysis, and least absolute shrinkage and selection operator (LASSO) regression, were utilized to screen for feature genes based on the Gene Expression Omnibus (GEO) dataset.

Collection methods of exhaled volatile organic compounds for lung cancer screening and diagnosis: a systematic review.

Background: The identification of volatile organic compounds (VOCs) in exhaled breath has garnered significant research attention as a means of screening and diagnosing lung cancer in recent decades. However, there is no universally accepted protocol for the collection of breath samples to measure VOCs in the clinical context. The purpose of this study was to summarize the current sampling techniques used to obtain VOCs from exhaled breath specifically in the context of lung cancer screening and diagnosis.

The therapeutic potential of recombinant ANGPTL4 in Parkinson's disease: Evidence from in vivo and in vitro studies.

Background: The established body of knowledge attests to the pivotal influence of ANGPTL4 on lipid metabolism and vascular biology. Nevertheless, its potential implication in neurodegenerative disease remains to be fully characterized.

Methods: The present investigation delves into the involvement of ANGPTL4 in the pathological progression of PD, both in vitro and in vivo.

Role and mechanism of myonectin in severe acute pancreatitis: a crosstalk between skeletal muscle and pancreas.

Background: Severe acute pancreatitis (SAP) is characterized by high mortality rates and various complications, including skeletal muscle atrophy, which significantly exacerbates its outcomes. Despite its clinical relevance, the mechanistic understanding of the relationship between skeletal muscle and the pancreas in SAP remains limited. Our study aimed to elucidate this "organ crosstalk" and its potential implications.

Identifying and validating the key regulatory transcription factor YY1 in the aging process of pancreatic beta cells based on bioinformatics.

The aging of pancreatic beta cells is closely associated with various diseases, such as impaired glucose tolerance, yet the underlying regulatory mechanisms remain unclear. In this study, we screened young and aged mouse pancreatic beta cells' high-throughput sequencing data from the GEO public database. Utilizing bioinformatics techniques, we identified the key regulatory factor YY1 in the aging process of pancreatic islets.

Inconsistencies in predictive models based on exhaled volatile organic compounds for distinguishing between benign pulmonary nodules and lung cancer: a systematic review.

Background: There is a general rise in incidentally found pulmonary nodules (PNs) requiring follow-up due to increased CT use. Biopsy and repeated CT scan are the most useful methods for distinguishing between benign PNs and lung cancer, while they are either invasive or involves radiation exposure. Therefore, there has been increasing interest in the analysis of exhaled volatile organic compounds (VOCs) to distinguish between benign PNs and lung cancer because it's cheap, noninvasive, efficient, and easy-to-use.