Evolutionary and Structural Analysis of the Aquaporin Gene Family in Rice.

Aquaporins in rice ( L.) represent a pivotal class of transmembrane channel proteins that mediate the bidirectional transport of water and small solutes, which have critical functions in cellular osmoregulation and ion homeostasis maintenance. Their evolutionary diversity and functional plasticity constitute fundamental mechanisms underlying the adaptive responses to diversified environmental challenges.

Conservation and divergence of OsGSTU9 and OsGSTU19 in cadmium detoxification and accumulation in rice.

Cadmium (Cd) is one of the heavy metals highly toxic to organisms and the increase of Cd contamination in crops forms an urgent issue to food safety and human health. Overproduction of Reactive Oxygen Species (ROS) is the primary response in plant cells subjected to Cd, which could be partially alleviated by Glutathione S-transferases (GSTs). Here, two rice GST genes, OsGSTU9 and OsGSTU19 belonging to the same GST subfamily, were identified due to their preferential expression in root and their significantly Cd-induced upregulation.

Structural and Functional Diversity of Glutamate Receptors-Like Channels in Plants.

Glutamate receptor-like (GLR) family encodes cation-permeable ion channels that are crucial for defense signaling and have attracted significant research interest. The identification of multiple GLRs subfamilies across vascular lineages suggests their functional diversity in plants. Functional studies of clade 3 GLRs confirm their critical role in generating electrical signals and increasing cytosolic Ca in response to mechanical wounding, insect and pathogen attacks, and other environmental cues for systemic defense responses.

Skillful multiyear prediction of flood frequency along the US Northeast Coast using a high-resolution modeling system.

Using tide gauge (TG) observations, we identify pronounced multidecadal fluctuations in sea level along the US Northeast Coast (USNEC) superimposed on a long-term increasing trend. This multidecadal sea level variability, largely arising from fluctuations in the buoyancy-driven Atlantic meridional overturning circulation (AMOC), substantially modulates the frequency of flood occurrences along the USNEC and serves as a source of multiyear predictability. Using an initialized dynamical downscaling decadal prediction system with a /° ocean resolution, we demonstrate that flood frequency along the USNEC can be predicted on multiyear to decadal timescales.

Genome-Wide Identification, Molecular Evolution, and Expression Divergence of , , , and Gene Family in Barley.

Organic and inorganic nutrients, osmotic components, associated protein cofactors, and signaling molecules regulate biotic and abiotic stresses in plants. Earlier reports suggest that plant cells activate anion channels and induce the efflux of anions at the plasma membrane under drought. Herein, (), (), (), and MECHANOSENSITIVE CHANNEL of SMALL CONDUCTANCE-LIKE (, ) gene family were reported in barley.

Genomic and evolutionary evidence for drought adaptation of allopolyploid Brachypodium hybridum.

Climate change is increasing the frequency and severity of drought worldwide, threatening the environmental resilience of cultivated grasses. However, the genetic diversity in many wild grasses could contribute to the development of climate-adapted varieties. Here, we elucidated the impact of polyploidy on drought responses using allotetraploid Brachypodium hybridum (B.

OsCaM1-1 Is Responsible for Salt Tolerance by Regulating Na/K Homoeostasis in Rice.

Calmodulin, a highly conserved calcium-binding protein, plays a crucial role in response to salt stress. Previous studies investigated sequence and function of calmodulin members in some plants, but their roles in rice have not been fully elucidated. Three OsCaM1 genes namely OsCaM1-1/2/3 encode the same OsCaM1 protein.

Efficient gene editing of a model fern species through gametophyte-based transformation.

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease (Cas) system allows precise and easy editing of genes in many plant species. However, this system has not yet been applied to any fern species through gametophytes due to the complex characteristics of fern genomes, genetics, and physiology. Here, we established a protocol for gametophyte-based screening of single-guide RNAs (sgRNAs) with high efficiency for CRISPR/Cas9-mediated gene knockout in a model fern species, Ceratopteris richardii.

Cr(VI) behaves differently than Cr(III) in the uptake, translocation and detoxification in rice roots.

Excessive accumulation of chromium (Cr) causes severe damage to both physiological and biochemical processes and consequently growth repression in plants. Hexavalent chromium [Cr(VI)]-elicited alterations in plants have been widely elucidated at either physiological or molecular level, whereas little is known about trivalent chromium [Cr(III)]. Here, we found that both Cr(III) and Cr(VI) significantly inhibited root growth in rice plants.

Stomatal evolution and plant adaptation to future climate.

Global climate change is affecting plant photosynthesis and transpiration processes, as well as increasing weather extremes impacting socio-political and environmental events and decisions for decades to come. One major research challenge in plant biology and ecology is the interaction of photosynthesis with the environment. Stomata control plant gas exchange and their evolution was a crucial innovation that facilitated the earliest land plants to colonize terrestrial environments.

Sesquiterpenoids from the roots of Lindera glauca and their cytotoxicity activities.

The chemical investigation of the roots of Lindera glauca guided the isolation and identification of 3 new sesquiterpenoids, namely glaucatotones J-L (1-3), and one known congener, (1β,5β)-1-hydroxyguaia-4(15),11(13)-dieno-12,5-lactone (4). The structures of new compounds were established based on comprehensive spectrographic methods, mainly including 1D & 2D NMR and HRESIMS analyses, and the absolute configurations were further confirmed by the comparison of experimental and calculated electronic circular dichroism. The cytotoxicity activities of isolates were evaluated, and the results showed that they have moderate cytotoxic activities.

Allene oxide synthase 1 contributes to limiting grain arsenic accumulation and seedling detoxification in rice.

Arsenic (As) is a cancerogenic metalloid ubiquitously distributed in the environment, which can be easily accumulated in food crops like rice. Jasmonic acid (JA) and its derivatives play critical roles in plant growth and stress response. However, the role of endogenous JA in As accumulation and detoxification is still poorly understood.

Molecular evolution and interaction of 14-3-3 proteins with H+-ATPases in plant abiotic stresses.

Environmental stresses severely affect plant growth and crop productivity. Regulated by 14-3-3 proteins (14-3-3s), H+-ATPases (AHAs) are important proton pumps that can induce diverse secondary transport via channels and co-transporters for the abiotic stress response of plants. Many studies demonstrated the roles of 14-3-3s and AHAs in coordinating the processes of plant growth, phytohormone signaling, and stress responses.

Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens.

In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens.

Spatiotemporal Epidemiology of the Gonorrhea Epidemic in Relation to Neighborhood-level Structural Factors in an Eastern Province of China, 2016-2020.

Context: Gonorrhea, a highly communicable, sexually transmitted infection, remains a major public-health concern globally. In recent years, Zhejiang province, an eastern province, has had the highest incidence of gonorrhea in China.

Objective: The study intended to identify the geographic distribution patterns and spaciotemporal clustering characteristics of the disease's incidence in Zhejiang between 2016 and 2020, to understand the spatial epidemiology of gonorrhea and to pinpoint the locations with relatively high risks of gonorrhea, the hotspots, which could be the key areas for disease prevention and control.

Molecular characterization of TNF-β and IFN-γ in yellowfin seabream (Acanthopagrus latus, Hottuyn, 1782) and their immune responses to density stress during transport.

The inflammatory cytokines TNF-β and IFN-γ are important mediators of the vertebrate inflammatory response and coordinators of the immune system in regard to NF-κB signalling pathways. In this study, the TNF-β and IFN-γ genes of yellowfin seabream, Acanthopagrus latus were identified, and the multiple sequence alignments, evolutionary relationships and gene expressions of the two genes were also determined. AlTNF-β contained a 762 bp open reading frame (ORF) encoding 253 amino acids, while AlIFN-γ contained a 582 bp ORF encoding 193 amino acids.

Hydrogen peroxide reduces root cadmium uptake but facilitates root-to-shoot cadmium translocation in rice through modulating cadmium transporters.

Cadmium (Cd) contamination in agricultural soils has become a serious worldwide environmental problem threatening crop production and human health. Hydrogen peroxide (HO) is a critical second messenger in plant response to Cd exposure. However, its role in Cd accumulation in various organs of plants and the mechanistic basis of this regulation remains to be elucidated.

Exploring lemology teaching with "internet plus" flipped classroom pedagogy.

Background: To investigate the use of flipped classroom pedagogy based on "Internet plus" in teaching viral hepatitis in the lemology course during the COVID-19 epidemic.

Methods: This study included students from the clinical medicine general practitioner class at Nanjing Medical University's Kangda College, with the observation group consisting of 67 students from the 2020-2021 school year and the control group consisting of 70 students from the 2019-2020 school year. The observation group used "Internet plus" flipped classroom pedagogy, while the control group used conventional offline instruction.

Evaluation of Isolates Susceptibility to Antibiotics in Zhejiang Province Since 2007.

Objective: This study aimed to assess the drug susceptibility of clinical isolates of to spectinomycin, ceftriaxone and azithromycin. Moreover, the temporal trends in the minimum inhibitory concentration (MIC) of five antibiotics from Zhejiang, China, are also in the scope of this study.

Methods: A total of 1710 gonococcal clinical strains were collected between 2007 and 2021 from health-care institutions in Zhejiang.

Calcium and L-glutamate present the opposite role in managing arsenic in barley.

Arsenic contamination in agricultural soils has posed tremendous threat to sustainable crop production and human health via food chain. Calcium and Glutamate have been well-documented in metal(loid)s detoxification, but it is poorly understood how they regulate arsenic-induced toxicity to plants. In this study, the effect of glutamate and calcium at high concentration on arsenic toxicity and accumulation in barley seedling was accessed in terms of plant growth, photosynthetic efficacy, arsenic uptake, translocation and accumulation, antioxidant defense, nutrient uptake and the expression of As transporters.

Physiological and transcriptomic evidence of antioxidative system and ion transport in chromium detoxification in germinating seedlings of soybean.

Chromium (Cr) toxicity impairs the productivity of crops and is a major threat to food security worldwide. However, the effect of Cr toxicity on seed germination and transcriptome of germinating seedlings of soybean crop has not been fully explored. In this study, two Cr-tolerant lines (J82, S125) and two Cr-sensitive ones (LD1, RL) were screened out of twenty-one soybean (Glycine max L.

Molecular Regulation and Evolution of Redox Homeostasis in Photosynthetic Machinery.

The recent advances in plant biology have significantly improved our understanding of reactive oxygen species (ROS) as signaling molecules in the redox regulation of complex cellular processes. In plants, free radicals and non-radicals are prevalent intra- and inter-cellular ROS, catalyzing complex metabolic processes such as photosynthesis. Photosynthesis homeostasis is maintained by thiol-based systems and antioxidative enzymes, which belong to some of the evolutionarily conserved protein families.

Molecular evidence for adaptive evolution of drought tolerance in wild cereals.

The considerable drought tolerance of wild cereal crop progenitors has diminished during domestication in the pursuit of higher productivity. Regaining this trait in cereal crops is essential for global food security but requires novel genetic insight. Here, we assessed the molecular evidence for natural variation of drought tolerance in wild barley (Hordeum spontaneum), wild emmer wheat (Triticum dicoccoides), and Brachypodium species collected from dry and moist habitats at Evolution Canyon, Israel (ECI).