Evaluation of dental implant with hydroxyapatite coating by laser-induced hydrothermal synthesis: in vitro and in vivo experimental study.

Various surface modification techniques have been developed to improve the survival rate of dental implants. This study aimed to evaluate both in vitro and in vivo outcomes of implants coated with a nano/micro-assembled hydroxyapatite (HA) structure using a laser-induced single-step coating (LISSC) technique. Four types of implant surfaces were examined: machined surface implants (MA), sandblasted large-grit acid-etched implants (SLA), resorbable blasting media implants (RBM), and HA-coated implants (HA).

A Wearable Electrochemical Biosensor for Salivary Detection of Periodontal Inflammation Biomarkers: Molecularly Imprinted Polymer Sensor with Deep Learning Integration.

The work presented here introduces a developed electrochemical biosensor for the salivary detection of matrix metalloproteinase-8 (MMP-8), utilizing a molecularly imprinted polymer (MIP) matrix based on poly(o-phenylenediamine). To enhance detection sensitivity and modulate impedance responses, graphene oxide (GO) is incorporated as an interlayer, providing a conductive and chemically stable matrix for precise electrochemical sensing. Density functional theory simulations confirm the formation of highly selective binding sites, further reinforcing the sensor's specificity for MMP-8 detection.

Combinatorial Glyoxal Cross-Linking and Glutamic Acid Capping for Mitigating Calcification in Bovine Pericardium.

Bioprosthetic heart valves (BHVs) offer advantages over mechanical valves but are limited by long-term degeneration and calcification. This study aimed to develop a durable BHV material using decellularized bovine pericardium (BP) and alternative cross-linking strategies. BP was decellularized using a combination of sodium deoxycholate (SDC) and Triton X-100 (TX), which removed cellular components while preserving the extracellular matrix (ECM), as confirmed by histological, DNA quantification, and biochemical analyses.

High spectral energy density all-fiber nanosecond pulsed 1.7 μm light source for photoacoustic microscopy.

We present a high spectral energy density all-fiber nanosecond pulsed 1.7 μm light source specifically designed for photoacoustic microscopy (PAM). The system targets the 1st overtone absorption of C-H bonds near 1720 nm within the near-infrared-III (NIR-III) window, where lipids exhibit strong optical absorption, and tissues benefit from reduced scattering and high permissible fluence.

Differentiation Defect Into GABAergic Neurons in Cerebral Organoids From Autism Patients.

Objectives: Autism spectrum disorder (ASD) is a neurodevelopmental condition that affects social communication and behaviors. While previous studies using animal models have substantially expanded our knowledge about ASD, the lack of an appropriate human model system that accurately recapitulates the human-specific pathophysiology of ASD hinders the precise understanding of its etiology and the development of effective therapies. This study aims to replicate pathological phenotypes in cerebral organoids derived from idiopathic ASD patients and to conduct proof-of-concept research for the development of ASD therapeutics.

Versatile and Marvelous Potentials of Polydeoxyribonucleotide for Tissue Engineering and Regeneration.

Over the past decade, substantial focus has been placed on polydeoxyribonucleotide (PDRN) due to its promising pharmacological properties, making it a valuable candidate for tissue engineering applications. Accordingly, this paper aims to review and summarize the latest experimental research on PDRN in the context of tissue engineering and regeneration. The unique biochemical mechanisms of PDRN to promote cellular behavior and regeneration are summarized.

Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous system.

Conventional understanding dictates that mammalian neural stem cells (NSCs) exist only in the central nervous system. Here, we report that peripheral NSCs (pNSCs) exist outside the central nervous system and can be isolated from mouse embryonic limb, postnatal lung, tail, dorsal root ganglia and adult lung tissues. Derived pNSCs are distinct from neural crest stem cells, express multiple NSC-specific markers and exhibit cell morphology, self-renewing and differentiation capacity, genome-wide transcriptional profile and epigenetic features similar to control brain NSCs.

Alterations in the Neuromuscular Control Mechanism of the Legs During a Post-Fatigue Landing Make the Lower Limbs More Susceptible to Injury.

Fatigue causes the lower limb to land in an injury-prone state, but the underlying neuromuscular control changes remain unclear. This study aims to investigate lower limb muscle synergies during landing in basketball players, both before and after fatigue, to examine alterations in neuromuscular control strategies induced by fatigue. Eighteen male recreational basketball players performed landing tasks pre- and post-fatigue induced by 10 × 10 countermovement jumps.

N-benzyl-N-methyldecane-1-amine derived from garlic ameliorates UVB-induced photoaging in HaCaT cells and SKH-1 hairless mice.

Skin tissue is susceptible to oxidative stress-induced senescence provoked by ultraviolet (UV) exposure in our daily lives, resulting in photoaging. Herein, we explore whether N-benzyl-N-methyldecan-1-amine (BMDA) derived from garlic ameliorates UVB-induced photoaging. To address this issue, HaCaT keratinocytes were exposed to UVB irradiation under BMDA treatment.

3D bioprintable Mg-incorporated hydrogels tailored for regeneration of volumetric muscle loss.

Current therapeutic approaches for volumetric muscle loss (VML) face challenges owing to limited graft availability and insufficient bioactivity. Three-dimensional (3D) bioprinting has become an alternative technology for fabricating native tissue-mimetic grafts, allowing for tailored structures and complex designs. We developed an Mg-incorporated bioink composed of thiolated gelatin (GtnSH) and maleimide-conjugated gelatin (GtnMI) decorated with magnesium peroxide (MgO), referred to as a GtnSH/GtnMI/MgO bioink.

Cellular Behaviors of Human Dermal Fibroblasts on Pyrolytically Stripped Carbon Nanofiber's Surface.

There has been limited exploration of carbon nanofiber as a scaffold for cellular attachment and proliferation. In this work, commercially available, pyrolytically stripped carbon nanofiber (cCNF) is deposited over electrospun nanofiber mats, polycaprolactone (PCL) and poly(D-lactide) (PDLA), to immobilize them and investigate whether the 3D cCNF layer's surface augments cell proliferation of human dermal fibroblasts (nHDF). Spectral characterizations, such as XRD and Raman, show that cCNF exhibited crystalline structure with a high graphitization degree.

Advancements in nanobiosensor technologies for in-vitro diagnostics to point of care testing.

Recently, the importance of rapid testing nanosensor technologies for in-vitro diagnostics (IVD) and point-of-care testing (POCT) is being increasingly recognized. Owing to their high sensitivity and rapidity, nanosensor-based diagnostic devices are evolving into self-diagnostic tools that enable real-time in-situ analyses. These advances have become the focus of the public health control system, not only to prevent the spread of infectious diseases but also to enable the early detection of critical diseases through continuous health monitoring technologies.

Tailoring photobiomodulation to enhance tissue regeneration.

Photobiomodulation (PBM), the use of biocompatible tissue-penetrating light to interact with intracellular chromophores to modulate the fates of cells and tissues, has emerged as a promising non-invasive approach to enhancing tissue regeneration. Unlike photodynamic or photothermal therapies that require the use of photothermal agents or photosensitizers, PBM treatment does not need external agents. With its non-harmful nature, PBM has demonstrated efficacy in enhancing molecular secretions and cellular functions relevant to tissue regeneration.

Extraction of γ-chitosan from insects and fabrication of PVA/γ-chitosan/kaolin nanofiber wound dressings with hemostatic properties.

A nanofiber-based composite nonwoven fabric was fabricated for hemostatic wound dressing, integrating polyvinyl alcohol (PVA), kaolin, and γ-chitosan extracted from three type of insects. The γ-chitosan extracted from Protaetia brevitarsis seulensis exhibited the highest yield at 21.5%, and demonstrated the highest moisture-binding capacity at 535.

Nanofibrous Material-Reinforced Printable Ink for Enhanced Cell Proliferation and Tissue Regeneration.

The three-dimensional (3D) printing of biomaterials, cells, and bioactive components, including growth factors, has gained interest among researchers in the field of tissue engineering (TE) with the aim of developing many scaffolds to sustain size, shape fidelity, and structure and retain viable cells inside a network. The biocompatible hydrogel employed in 3D printing should be soft enough to accommodate cell survival. At the same time, the gel should be mechanically strong to avoid the leakage of cells into the surrounding medium.

DOT1-like histone lysine methyltransferase is critical for adult vessel maintenance and functions.

Objective: Disruptor of telomeric silencing 1-like (DOT1L) is the only known histone H3K79 methyltransferase essential for the development of the embryonic cardiovascular system, including the heart, blood vessels, and lymphatic vessels, through transcriptional regulation. Our previous study demonstrated that Dot1l deletion results in aberrant lymphatic development and function. However, its precise function in the postnatal cardiovascular system remains unknown.

Cold-Responsive Hyaluronated Upconversion Nanoplatform for Transdermal Cryo-Photodynamic Cancer Therapy.

Cryotherapy leverages controlled freezing temperature interventions to engender a cascade of tumor-suppressing effects. However, its bottleneck lies in the standalone ineffectiveness. A promising strategy is using nanoparticle therapeutics to augment the efficacy of cryotherapy.

Skeletal muscle regeneration with 3D bioprinted hyaluronate/gelatin hydrogels incorporating MXene nanoparticles.

There has been significant progress in the field of three-dimensional (3D) bioprinting technology, leading to active research on creating bioinks capable of producing structurally and functionally tissue-mimetic constructs. TiCT MXene nanoparticles (NPs), promising two-dimensional nanomaterials, are being investigated for their potential in muscle regeneration due to their unique physicochemical properties. In this study, we integrated MXene NPs into composite hydrogels made of gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA) to develop bioinks (namely, GHM bioink) that promote myogenesis.

RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos.

Objective: R-loops are DNA:RNA triplex hybrids, and their metabolism is tightly regulated by transcriptional regulation, DNA damage response, and chromatin structure dynamics. R-loop homeostasis is dynamically regulated and closely associated with gene transcription in mouse zygotes. However, the factors responsible for regulating these dynamic changes in the R-loops of fertilized mouse eggs have not yet been investigated.