Combinatorial Glyoxal Cross-Linking and Glutamic Acid Capping for Mitigating Calcification in Bovine Pericardium.

Bioprosthetic heart valves (BHVs) offer advantages over mechanical valves but are limited by long-term degeneration and calcification. This study aimed to develop a durable BHV material using decellularized bovine pericardium (BP) and alternative cross-linking strategies. BP was decellularized using a combination of sodium deoxycholate (SDC) and Triton X-100 (TX), which removed cellular components while preserving the extracellular matrix (ECM), as confirmed by histological, DNA quantification, and biochemical analyses.

Biopolymer collagen-chitosan scaffold containing Aloe vera for chondrogenic efficacy on cartilage tissue engineering.

The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes in a standard condition. Cytocompatibility was analyzed using fluorescent dyes (calcein AM/ethidium bromide) and the viable cells were quantified by MTT assay. Glycosaminoglycan (GAG) content of ECM was estimated by using 1, 9-Dimethyl methylene Blue (DMMB).