Prefoldin complex promotes interferon-stimulated gene expression and is inhibited by rotavirus VP3.

Timely induction of interferons and interferon-stimulated genes (ISGs) is critical for successful host defense against viral infections. VP3 encoded by rotavirus is implicated in interferon antagonism. However, the precise mechanisms remain incompletely understood.

Enzymatic craftsmanship in collagen glycosylation.

Collagen IV, a key structural protein of basement membranes, is vital for the development and survival of multicellular life forms. Its maturation relies on specific post-translational O- glycosylation, primarily mediated by lysyl hydroxylase-3 (LH3) and other glycosyltransferases, which sculpt collagen by modifying the hydroxylysine residues with sugar moieties.

Global analysis of the Hfq-mediated RNA interactome discovers a MicA homolog that affects the cytotoxicity, biofilm formation, and resistance to complement of Bordetella pertussis.

Bordetella pertussis is a Gram-negative, strictly human re-emerging respiratory pathogen and the causative agent of whooping cough. The requirement of the RNA chaperone Hfq for the virulence of B. pertussis suggests that Hfq-dependent small regulatory RNAs (sRNAs) are involved in the virulence of this pathogen.

Nipah virus: pathogenesis, genome, diagnosis, and treatment.

The highly infectious Nipah virus (NiV) is classified under the Paramyxoviridae family and is categorized under the genus Henipavirus. NiV spreads to humans through zoonotic transmission from reservoir host bats and other intermediate hosts. It is highly contagious and has a high case fatality rate (CFR) of ~ 40-80%.

Long-term Exposure to Complementary and Alternative Medicine Leads to Neural Epidermal Growth Factor-like 1 Membranous Nephropathy.

Introduction: Membranous nephropathy is a type of glomerular disease which results in nephrotic syndrome that can be seen in adults. In most cases of membranous nephropathy, the target antigen has been phospholipase A2 receptor (PLA2R). In recent times, another antigen named neural epidermal growth factor-like 1 (NELL-1) has been labeled.

Purification, refolding, and pH-dependent stability evaluation of Zika virus EDIII protein.

Zika virus (ZIKV) infection, primarily spread through mosquito bites, poses a significant threat as global temperatures continue to rise. While vector control remains the primary strategy for mitigation, the design and development of cost-effective vaccines are essential to prevent future outbreaks, particularly in low- and middle-income countries. In our study, we have produced recombinant ectodomain III (rEDIII) of ZIKV from the E.

Genomic and Structural Insights into Chandipura Virus: A Comparative in Silico Analysis of Indian and West African Strains and Potential Host Receptor Interactions.

Chandipura virus (CHPV), a member of the Rhabdoviridae family, is an emerging pathogen responsible for encephalitis outbreaks, particularly among children under 15 years of age. Predominantly transmitted by sandflies, CHPV has caused multiple outbreaks in India, whereas strains from West African countries, including Kenya, Senegal, and Nigeria, have not been linked to human infections. The CHPV glycoprotein (G protein), a crucial spike protein, plays a key role in viral entry into host cells.

The evolving landscape of Chandipura virus: A comprehensive account of outbreaks to recent advances.

Chandipura virus (CHPV), a member of the family Rhabdoviridae, has garnered attention due to its significant implications on human health, particularly in India, where it has contributed to encephalitis outbreaks. This review provides a full-spectrum analysis of CHPV, detailing its origin, historical context, and geographical distribution, which primarily spans India and parts of Africa. CHPV is predominantly transmitted through infected sandflies, although alternative transmission routes cannot be overruled.

Kyasanur Forest disease virus non-structural protein NS1 forms multimers in solution, with a distinctly identifiable tetrameric state.

Kyasanur Forest Disease Virus (KFDV), a flavivirus, is predominantly present in the tropical region of southern India and is responsible for viral hemorrhagic disease in primates and non-primate animals. KFDV infection is spread by tick bites. The other medically important viruses of Flaviviridae family are dengue (DENV), Zika (ZIKV), West Nile virus (WNV) and Japanese encephalitis virus (JEV).

Risk Factors, Trends, and Financial Impact for 30-Day Unplanned Readmissions in Patients Admitted With Myocarditis and COVID-19: Insights From the Healthcare Cost and Utilization Project (HCUP) Nationwide Readmission Database.

Background: The coronavirus disease 2019 (COVID-19) outbreak was first documented in Wuhan, China, in December 2019. Myocarditis, an inflammatory condition characterized by swelling and thickening of the heart muscle, has been linked to severe COVID-19 cases, contributing to worse clinical outcomes. The SARS-CoV-2 virus enters human cells through angiotensin-converting enzyme 2 (ACE2), and myocardial involvement can result from direct viral invasion, hyperinflammation, and immune-mediated damage.

Left Bundle Branch Pacing in a Patient With Dextroposed Heart: A Case Report.

The Right Ventricular (RV) apex has been the standard site for pacing in symptomatic bradyarrhythmias, but chronic RV pacing can cause adverse effects such as atrial arrhythmias and left ventricular dysfunction. Physiological pacing, including His bundle and left bundle pacing, offers alternatives with fewer complications. We present a 66-year-old male with a dextroposed heart and fibrotic right lung requiring left bundle branch pacing due to a high RV pacing burden.

Cardiac evaluation in patients awaiting kidney transplant-position statement of the Cardiological Society of India and Indian Society of Nephrology.

Cardiovascular diseases are a major cause of death after kidney transplantation. This statement addresses preoperative cardiac decision-making and management with the aim of assessing and reducing the risk of the kidney transplant surgery. Important issues from a clinician's perspective include the basic cardiovascular workup of these patients, coronary evaluation and management of coronary artery disease, valvular heart disease and left ventricular systolic dysfunction.

Optimized high-yield expression of envelope glycoprotein domain III from dengue virus serotypes 1 to 4.

Dengue virus (DENV) envelope glycoprotein Domain III (EDIII) is critical for viral entry, highly immunogenic, and induces robust neutralizing antibody response. It is a prominent candidate for designing subunit-based vaccines and can also be harnessed as an antigenic bait for isolation of neutralizing human mAbs. Here, we describe an optimized method for high-yield expression of recombinant domain EDIII protein from DENV serotypes 1 to 4 in different Escherichia coli (E.

Purification and characterization of kyasanur forest disease virus EDIII domain of major envelope glycoprotein.

Current research efforts are underway to create novel approaches for the efficient diagnosis, monitoring, and mitigation of Kyasanur Forest Disease Virus (KFDV) infections. Flavivirus subunit-based vaccines based on envelope glycoprotein EDIII are now in preclinical and clinical research stages. Efficient purification and isolation methods for surface immunogenic viral antigens, including the recombinant envelope immunoglobulin-like domain III (rEDIII) protein, are crucial for the production and manufacturing of promising vaccine candidates that have been extensively assessed in previous literature.

Intact Parathyroid Hormone (iPTH) Assay: An Early Approach for Bone Health Assessment in Chronic Renal Failure.

Background: Chronic kidney disease (CKD) is a global public health burden with significantly increasing mortality. Chronic kidney disease leads to various complications, including anemia, cardiovascular complications, salt and water retention, metabolic acidosis, electrolyte disorders, and chronic kidney disease-mineral and bone disorder (CKD-MBD), amongst which CKD-MBD is a particularly important complication that develops early.

Materials And Methods: In the present study, various biochemical analytes were assessed for their significance in monitoring the CKD-MBD in different stages of CKD.

Factors influencing outcome in patients with intramedullary spinal cord tumors undergoing resective surgery.

Background: We evaluated whether preoperative functional status influenced surgical outcomes for patients with intramedullary spinal cord tumors (IMSCT).

Methods: We analyzed whether lower preoperative McCormick scores impacted primary outcomes for 78 consecutive patients with IMSCT of World Health Organization (WHO) grades I and II undergoing tumor resection between 2010 and 2018.

Results: Patients averaged 33.

A Comparative Study of Magnesium Sulfate, Lignocaine, and Propofol for Attenuating Hemodynamic Response During Functional Endoscopic Sinus Surgery Under General Anaesthesia: A Prospective Randomized Trial.

Objective: This study functional endoscopic sinus surgery (FESS) is a surgical procedure requiring minimal bleeding to optimize the surgical field. This study aimed to evaluate the effectiveness of magnesium sulfate, lignocaine, and propofol in attenuating hemodynamic response. The primary objective of this study was to compare the efficacy of these agents in reducing hemodynamic response.

Thoracic spinal anaesthesia - An effective alternative to general anaesthesia in breast surgeries: A randomised, non-blinded study.

Background And Aims: General anaesthesia (GA) is the preferred modality for breast surgeries; however, neuraxial anaesthesia can be performed in cases where GA poses a significant risk. We hypothesise that neuraxial blockade is a safe and effective alternative to GA in short-duration breast surgeries.

Methods: This randomised study included 30 patients of the American Society of Anesthesiologists physical status I and II, who were scheduled for elective breast surgeries of a duration of less than 90 min.

Clinical safety and performance of the world's thinnest-strut Evermine50 everolimus-eluting stent: a 24-month follow-up of the Evermine 50 EES - 1 study.

Background: Ultrathin-strut stents are considered the future of percutaneous coronary intervention for treating coronary artery disease (CAD). These drug-eluting stents with biodegradable-polymer technology have the potential to improve clinical outcomes in CAD patients.

Aims: This study aimed to evaluate the safety and performance of newer-generation ultrathin-strut (50 µm) Evermine50 everolimus-eluting stents (EES) in patients with single or multiple long lesions.

Comparative Evaluation of Microhardness and Polymerization Shrinkage in Residual Zirconia Reinforced Provisional Restorations: An In Vitro Study.

Aim The aim of this study is to evaluate and compare the microhardness and polymerization shrinkage of polymethyl methacrylate reinforced with residual zirconia. Materials and methods A total of 360 resin samples were fabricated, with dimensions of 12 mm × 12 mm × 3 mm and 12 mm × 8 mm × 6 mm. Zirconia dust (40% by weight) was added to 180 of these samples.