High-Throughput Quantification of Pesticide Residues in Complex Matrix (Chilli Powder) Using Liquid Chromatography Tandem Mass Spectrometry: Inter- and Intra-Day Validation.

Background: Chilli powder is a widely consumed spice, however during cultivation of chilli crops are often subjected to pesticide treatments to control pests and diseases. Thus, monitoring pesticide residues in such matrices is crucial for food safety and to comply with national as well as international regulations for export/import purposes. However, accurate analysis of pesticide residue in chilli powder is challenging due to its complex nature.

Biofuel production from starchy crops: advanced technology and current perspectives.

The sustainable replacement of conventional fossil fuels with bioethanol produced from starchy crops has garnered significant interest. This review paper provides current perspectives in the field and an overview of advanced technologies, emphasising the importance of efficient and environmentally friendly processes. Starchy crops such as wheat, barley, cassava, potato, and maize possess high starch content, which serves as a key feedstock for bioethanol production.

Determination of the optimal ejaculate concentration of a buffalo bull for successful semen cryopreservation.

For cattle bulls, only ejaculates with a sperm concentration of 500 million/mL or higher are selected for sperm cryopreservation. There is no established ejaculate's minimum sperm concentration threshold for buffalo semen cryopreservation. Therefore, the first objective of the study was to determine the percentage of ejaculates of buffalo bulls having concentrations lower than 500 million/mL, the percentage of ejaculates suitable for cryopreservation across different concentrations of donated ejaculates, and to estimate the impact of ejaculate concentrations on the production of semen doses.

Use of R848, a TLR 7/8 agonist to separate X and Y spermatozoa in buffalo: effects on cleavage rates, blastocyst production, and the ratio of male to female embryos.

The study aimed to separate X and Y buffalo sperm using R848, a TLR7/8 agonist. Semen from three Murrah buffalo bulls were collected and divided into aliquots for swim-up in modified human tubal fluid (mHTF) medium with varying concentrations of R848 (0, 0.03, 0.

Evaluation of Reference Values for the Patellotrochlear Index in the North Indian Population: A Cross-sectional Magnetic Resonance Imaging-based Study.

Background: The patella plays a crucial role in the extensor mechanism of the knee joint, significantly enhancing the efficiency of the quadriceps muscle. The alignment and height of the patella are critical for maintaining knee joint functionality and preventing knee-related disorders. The Patellotrochlear Index (PTI) is a novel measurement method that provides a more accurate assessment of patellar height and its relationship with the femoral trochlea, particularly in the North Indian population.

Outcomes of posterior wall acetabular fractures with marginal impaction using greater trochanter autografting: A prospective cohort study.

Background: Posterior wall acetabular fractures with marginal impaction pose significant challenges, mainly because of the risk of joint incongruity and the potential for developing post-traumatic arthritis. Restoration of the articular surface and stability are essential to achieve good outcomes. This study investigates the surgical outcomes of posterior wall acetabular fractures with marginal impaction using the Kocher-Langenbeck (KL) approach, buttress plating, and greater trochanter autografting.

Analytical Techniques as Indicators of Biomarkers in Proteomics Cancer Diagnosis.

Background: Cancer is a complex disease marked by changes in the levels and functions of key cellular proteins, including oncogenes and tumor suppressors. Proteomics technology enables the identification of crucial protein targets and signaling pathways involved in cancer cell proliferation and metastasis. Various proteomics techniques have been employed to investigate the molecular mechanisms of cancer, aiding in the confirmation and characterization of heritable disorders.

Risk of Cardiovascular Diseases Associated with PCOS in India: A Review.

In the modern world, Polycystic Ovary Syndrome (PCOS) is thought to be the most prevalent endocrine condition affecting women. Compared to their normal counterparts, PCOS patients have higher rates of morbidity and death because they are more susceptible to these anomalies from an early age. Cardiovascular disease (CVD) and PCOS are prevalent in women.

Sustained virological response in HCV patients receiving antiviral treatment at a teaching centre of northern India.

In India, it is estimated that 10-24 million people are living with active HCV infection, with a seroprevalence of 0.09% to 2.02% among the healthy population.

A novel paradigm in the prediction of the performance of breeding buffalo bulls: role of hair cortisol and testosterone.

Despite having strong pedigree records, passing breeding soundness evaluations, and receiving appropriate management and nutrition, some breeding bulls consistently underperform in similar environmental conditions where other bulls thrive. These underperforming bulls are economically unsuitable for semen stations that produce semen doses. Our study aims to predict the performance of breeding bulls based on hair cortisol and testosterone concentrations.

Phytochemical and Phytopharmacological Insights into Argemone mexicana: Integrative Approaches in Eastern Indian Region as Folk Medicine.

Introduction: Argemone mexicana, commonly known as the Mexican poppy, has earned significant recognition in the traditional healing systems of the Eastern Indian region, where it has been used for centuries as a folk remedy for a variety of diseases. The study was based on search or identified through Pubmed, Google Scholar, Science Direct, etc. Meta-analysis: Phytochemical analysis has demonstrated that the alkaloids present in A.

Intraoperative complications in acetabular fracture management using the Stoppa approach: A retrospective cohort study.

Aim: The Aim of the study is to retrospectively analyse intraoperative complications in acetabular fractures treated via the Stoppa approach, identify their frequency and nature, and define potential risk factors, in addition to evaluating clinical outcomes.

Methods: This retrospective cohort study included a total of 136 acetabulum fracture cases (aged 16 to 82 years) that were managed using Stoppa approach. Records related with age, sex, mode of injury, body mass index, history and type of associated injury and time gap between injury and surgery were noted.

Repurposing brucine as a chemopreventive agent in mammary gland carcinoma: Regulating lactate transport through MCT-4.

In the present study, we aim to identify a potential drug candidate that targets the Monocarboxylate Transporter-4 (MCT-4) protein. Syrosingopine (SRY) is a well-established inhibitor of lactate transport through MCT-4. We screened 2,11,192 potential leads through ZINC database, which were atleast 50 % structurally similar with SYR.

Advancing siRNA Therapeutics targeting MCT-4: A Multifaceted approach integrating Arithmetical Designing, Screening, and molecular dynamics validation.

Monocarboxylate transporter 4 (MCT-4) is involved in various metabolic processes which are crucial in maintaining cellular pH and energy metabolism, and thus influence the tumor microenvironment. The study is aimed to rationally design effective Small interfering RNA (siRNA) that can silence MCT-4. We utilized a comprehensive workflow integrating multiple tools such as siDirect version 2.

Pre- and post-intervention oesophageal pressure topography metrics in the three subtypes of achalasia: A pilot study on Indian adults.

Background: Achalasia is characterized by failure of relaxation of the lower oesophageal sphincter (LOS) and abnormal peristalsis during swallowing. The study aimed to observe the effects of intervention in three sub-types of achalasia and compare the intervention outcomes among the three sub-types.

Methods: Forty-one patients underwent Eckardt scoring for severity of achalasia, followed by high-resolution manometry (HRM).

Bioethanol production from potatoes in India: A SWOT analysis.

India is the third-largest energy consumer globally, heavily reliant on imported oil. Projections suggest India's energy consumption will double by 2050, posing challenges to energy security and leading to significant foreign currency outflows. The extensive use of fossil fuels increases carbon emissions, raising environmental and health concerns.

Design, synthesis, molecular dynamics and gene silencing studies of novel therapeutic HIF-1α siRNAs in hypoxic cancer cells.

Hypoxia inducible factors (HIFs) are heterodimeric proteins that belong to a small group of transcription factors, which mainly regulates transcription of genes under hypoxic conditions. Particularly, oxygen sensing subunit of HIF-1α is a predominant subtype that heterodimerizes with oxygen-independent HIF-1β subunit, to trigger the transcription of hypoxia responsive genes. Due to poor supply of blood and rapid division of cancerous cells, tumor microenvironment exhibits low oxygen condition and therefore increased levels of HIF-1α.

Study of Correlation between Serum Vitamin B12 Level and Aberrant DNA Methylation in Infertile Males.

Introduction: Altered DNA methylation pattern in sperms has been associated with infertility in males demonstrating defective spermatogenesis or low semen quality. Vitamin B-12, by affecting 1-carbon metabolism pathways, might alter the DNA methylation pattern. We aimed to study the correlation of serum vitamin B12 levels with aberrant DNA methylation in infertile male patients.