Aquaporin (AQP) water channels facilitate fluid transport across cell membranes, are implicated in rodent oral water sensing, and were examined in the human tongue for their modulation by mouthfeel compounds and ensuing effects on perception and saliva tribology. Immunohistochemistry demonstrated abundant AQP1 and AQP2 and moderate AQP5 in human tongue and high AQP5 in salivary gland. Trained human tasting panels evaluated astringency intensities; results correlated with wine tannin measures and inhibition of AQP1 water flux ( ≥ 0.
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July 2025
Proline is an abundant wine metabolite shown to impart sweetness, viscosity, and increase flavour intensity. While several techniques are available to quantitate proline in wine, they are either costly, laborious, or prone to severe interferences. Proton nuclear magnetic resonance (H NMR) analysis of wine has provided a method for rapidly measuring several important wine metabolites, including proline.
View Article and Find Full Text PDFThere has been an increase in the consumption of plant-based milk alternatives (PBMA), though these often differ in sensory properties compared to cow milk. Some consumers specifically seek PBMA with sensory qualities that resemble cow milk; however, such products are not yet available on the market. This study compared the chemical composition, particle size, color, viscosity, physical stability, and volatile compounds of seven commercial milk types: almond, coconut, oat, rice, soy, plant-hybrid (a blend of plant-based ingredients), and cow milk.
View Article and Find Full Text PDFMultiple analytical methodologies allow quantitation of HS and methanethiol (MeSH) in wine, but confirmation that the determined concentrations are related to perceived off-aromas, or "reductive" faults, is yet to be provided. Fifty white wines underwent sensory evaluation and measurement of free and salt-treated HS and MeSH concentrations by gas chromatography with sulfur chemiluminescence detection and/or gas detection tubes. The determined concentrations were compared across techniques and different analysis laboratories.
View Article and Find Full Text PDFThe higher alcohols 2-phenylethanol, tryptophol, and tyrosol are a group of yeast-derived compounds that have been shown to affect the aroma and flavour of fermented beverages. Five variants of the industrial wine strain AWRI796, previously isolated due to their elevated production of the 'rose-like aroma' compound 2-phenylethanol, were characterised during pilot-scale fermentation of a Chardonnay juice. We show that these variants not only increase the concentration of 2-phenylethanol but also modulate the formation of the higher alcohols tryptophol, tyrosol, and methionol, as well as other volatile sulfur compounds derived from methionine, highlighting the connections between yeast nitrogen and sulfur metabolism during fermentation.
View Article and Find Full Text PDFChanges to Australian regulations now allow the limited addition of water to high-sugar musts pre-fermentation. In light of these changes, this study explored how water addition affects Shiraz wine composition and sensory properties. Wines were made from grapes at ≈13.
View Article and Find Full Text PDFIn most yeast-driven biotechnological applications, biomass is separated from the aqueous phase after fermentation or production has finished. During winemaking, yeasts are removed after fermentation by racking, filtration, or centrifugation, which add costs to the overall process and may reduce product yield. Theoretically, clarification and filtration can be aided through use of yeast strains that form flocs due to cell-cell binding, a process known as flocculation.
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