Robust, automated quantitation of proline in wine.

Proline is an abundant wine metabolite shown to impart sweetness, viscosity, and increase flavour intensity. While several techniques are available to quantitate proline in wine, they are either costly, laborious, or prone to severe interferences. Proton nuclear magnetic resonance (H NMR) analysis of wine has provided a method for rapidly measuring several important wine metabolites, including proline.

Assessment of PFDA toxicity on RTgill-W1 cell line via metabolomics and lipidomics approaches.

Perfluorodecanoic acid (PFDA), a long-chain perfluoroalkyl substance (PFAS), is known for its environmental persistence and potential toxicity. This study evaluated PFDA toxicity in the RTgill-W1 cell line, a model for aquatic toxicology, using a combination of cell viability assays, reactive oxygen species (ROS) measurements, and high-throughput metabolomics and lipidomics. PFDA exposure resulted in significant, dose-dependent reductions in cell viability and increased ROS production, with an EC₅₀ value of 51.

Metabolic disruptions induced by low concentrations of DMSO in RTgill-W1 fish cells: The importance of solvent controls in in vitro studies.

Dimethyl sulfoxide (DMSO) is a widely used solvent in biological research due to its ability to enhance membrane permeability, facilitating drug delivery and molecular transport across cellular membranes. However, its effects on cellular metabolism, especially at low concentrations, remain insufficiently understood. This study investigated the metabolic disruptions induced by 0.

Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion.

Background: Raspberry ketone is the primary aroma compound found in raspberries and naturally derived raspberry ketone is a valuable flavoring agent. The economic incentives for the production of raspberry ketone, combined with the very poor yields from plant tissue, therefore make this compound an excellent target for heterologous production in synthetically engineered microbial strains.

Methods: A de novo pathway for the production of raspberry ketone was assembled using four heterologous genes, encoding phenylalanine/tyrosine ammonia lyase, cinnamate-4-hydroxlase, coumarate-CoA ligase and benzalacetone synthase, in an industrial strain of Saccharomyces cerevisiae.

Accumulation of Glycoconjugates of 3-Methyl-4-hydroxyoctanoic Acid in Fruits, Leaves, and Shoots of Vitis vinifera cv. Monastrell following Foliar Applications of Oak Extract or Oak Lactone.

Grapevines are capable of absorbing volatile compounds present in the vineyard during the growing season, and in some cases, volatiles have been found to accumulate in fruits or leaves in glycoconjugate forms, that is, with one or more sugar moieties attached. The presence of oak lactone in wine is usually attributable to oak maturation, but oak lactone has been detected in wines made with fruit from grapevines treated with oak extract or oak lactone. This study investigated the accumulation of glycoconjugates of 3-methyl-4-hydroxyoctanoic acid (i.

Formation of Damascenone under both commercial and model fermentation conditions.

The fermentations, at a commercial winery, of six different grape musts encompassing the varieties Riesling, Chardonnay, Sauvignon blanc, Shiraz, Grenache, and Pinot noir were monitored for damascenone concentration. In every case, the concentration of damascenone increased during fermentation from low or undetectable levels to concentrations of several parts per billion. Further increases in damascenone concentration were observed during barrel aging of three of these wines.