Systemic Delivery of an mRNA-Encoding, Tumor-Activated Interleukin-12 Lock to Eliminate Tumors and Avoid Immune-Related Adverse Events.

Interleukin-12 (IL-12) is a robust proinflammatory cytokine that activates immune cells, such as T cells and natural killer cells, to induce antitumor immunity. However, the clinical application of recombinant IL-12 has been limited by systemic immune-related adverse events (irAEs) and rapid degradation. To address these challenges, we employed mRNA technology to encode a tumor-activated IL-12 "lock" fusion protein that offers both therapeutic efficacy and systemic safety.

Folate-Modified Smart Responsive Nanosystems for Enhancing Anti-Tumor Therapy Through Calcium Overload and Chemotherapy.

Objective: The combination of DOX and 5-Fu is an important chemotherapeutic regimen but lacks targeting to solid tumor sites. Precise drug delivery via folate-modified nanomaterials is an important measure to improve efficacy and reduce toxicity.

Methods: CaCO nanoparticles served as the carrier for loading DOX and 5-Fu, followed by encapsulation with folic acid-modified polydopamine (PDA) to form a smart dual drug-carrying nanosystem called FA-DCFP.

Structural basis and affinity improvement for an ATP-binding DNA aptamer.

DNA aptamers that bind small molecules with high affinity have revolutionized the fields of biosensing and bioimaging. Recently, a DNA aptamer named 1301b has been identified as the most potent DNA aptamer for the binding of adenosine triphosphate (ATP) with a dissociation constant () of ~2.7 µM.

Protamine-Facilitated Same-Day Discharge Following Distal Transradial Angiography.

Background: Despite growing adoption of distal transradial approach (dTRA), data on same-day discharge with protamine-assisted hemostasis following coronary angiography (CAG) remain limited. This pilot study investigated its safety and feasibility.

Methods: From January 2022 to September 2024, a total of 34 patients who were discharged on the same-day after elective CAG via dTRA in our center were included, as well as 34 matched patients who underwent CAG via standard transradial approach (sTRA) during the same period.

GZR101, a Novel Premixed Insulin for the Treatment of Diabetes Mellitus.

Inadequate glycemic control in patients with current premixed insulin regimens is often due to the short half-life and pronounced peak-to-trough ratio of basal insulin. This study evaluated insulin GZR33 (GZR33), an ultra-long basal insulin, and insulin GZR101 (GZR101), which is a premix of GZR33 and postprandial insulin aspart. The binding affinity of GZR33 to the human insulin receptor (hIR) was analyzed using surface plasmon resonance.

Dynamic covalent bonding based polysaccharide hydrogels and their applications in wound dressings: A review.

Polysaccharide hydrogels have emerged as pivotal wound dressings due to their inherent biocompatibility and extracellular matrix-mimicking properties. However, conventional systems employing permanent covalent or physical crosslinks suffer from irreversibility, poor self-repair capacity, and limited responsiveness that compromise clinical efficacy. To address these challenges, dynamic covalent bonding strategies have been innovatively integrated into polysaccharide networks, enabling unprecedented functionalities through reversible bond reconfiguration.

Development of Brassica rapa-nigra monosomic alien addition lines: cytology, genetics, and morphology.

Brassica rapa-nigra monosomic alien addition lines (MAALs) were developed and compared with B. oleracea-nigra MAALs containing the same B-genome chromosomes. The diploid Brassica nigra (black mustard) is the donor of the B-genome for two allotetraploids: B.

Effect of Saccharomyces boulardii supplementation to bismuth quadruple therapy on Helicobacter pylori eradication.

Background: Helicobacter pylori (H. pylori) infection is a common chronic infection, and there are over half of the global population infected with H. pylori.

Predictive Value of Inflammation Markers for Frailty in Older Patients with CVD.

Background: Chronic inflammation plays a pivotal role in the development of frailty in patients with cardiovascular diseases (CVD). Systemic inflammatory response index (SIRI) has been shown to reflect the overall inflammatory status. This study aimed to investigate the relationship between SIRI and frailty in older patients with CVD, and to develop a nomogram for predicting the risk of frailty in this population.

The Multi-Pistil Phenomenon in Higher Plants.

Correct floral morphology determines the accuracy of fruit formation, which is crucial for reproductive success in higher plants. Despite this, an abnormal, multi-pistil phenotype has been observed in the flowers of many plants. In this review, we gather information on the multi-pistil phenomenon in various species and highlight potential causes, as well as possible consequences, of the trait.

The role of SOX18 in nasopharyngeal carcinoma: implications for prognosis and therapy.

Objective: To investigate the cellular function of SOX18 in nasopharyngeal carcinoma (NPC) by analyzing its effects on tumor cell proliferation, apoptosis, migration and invasion, and to verify its expression and prognostic significance by clinical samples, thereby providing a basis for precise diagnosis and treatment.

Methods: SOX18 expression was analyzed in NPC cell lines and clinical samples. Gene silencing techniques were utilized to reduce SOX18 expression in NPC cells, followed by assays to evaluate cell proliferation, apoptosis, migration, and invasion.

Pharmacokinetics, Pharmacodynamics, and Safety Evaluation of the Novel HIF-PH Inhibitor Enarodustat: An Open-Label Phase I Study in Healthy Chinese Participants.

Background And Objectives: Enarodustat is a hypoxia-inducible factor-prolyl hydroxylase inhibitor. We evaluated the pharmacokinetics, pharmacodynamics, and safety profile of domestic enarodustat (SAL-0951) and analyzed the influence of ethnic factors.

Methods: In this phase I study, healthy Chinese participants received single and multiple oral doses (1, 5, and 15 mg) of SAL-0951 while in a fasted state.

Functional SELEX and Biomedical Applications of Aptamers: Beyond Molecular Recognition.

Aptamers, developed through SELEX (systematic evolution of ligands by exponential enrichment), are generally short oligonucleotide molecules with remarkable specificity and binding affinity to diverse biological targets. These molecules have shown promise across such fields as biosensing, molecular diagnostics, and bioimaging. However, while conventional aptamer selection strategies predominantly emphasize binding affinity, they overlook the broader spectrum of potential biological functionalities.

The WRKY28-BRC1 Transcription Factor Module Controls Shoot Branching in .

The trade-off between growth and defense is common in plants. We previously demonstrated that BnaA03.WRKY28 weakened resistance strength but promoted shoot branching in (rapeseed).

Beyond species and spatial boundaries: Enabling long-distance gene silencing in plants via guanidinium-siRNA nanoparticles.

RNA interference (RNAi) has been widely used in agriculture. However, it is well accepted that common methods of plant RNAi are species-dependent and lack systematic efficiency. This study designed a thiolated siRNA nanoparticle, guanidinium (Gu)-containing disulfide assembled siRNA (Gu-siRNA), demonstrating remarkable species independence and efficient systemic gene silencing across different plant species.

Safety analysis of brachial artery sheath removal after heparin reversal with a half dose of protamine after percutaneous coronary intervention: a single-center experience.

Aim: To evaluate the safety of brachial artery (BA) sheath removal after heparin neutralization with a half dose of protamine immediately after percutaneous coronary intervention (PCI).

Methods: The clinical data of 209 consecutive patients who underwent PCI through the BA at Fu Wai Hospital between September 2019 and June 2024 were retrospectively collected. In group I, the brachial sheath was removed 4 h after the PCI procedure.

DNA Self-Assembly Generated by Aptamer-Triggered Rolling Circle Amplification Cascades for Profiling Colorectal Cancer-Derived Small Extracellular Vesicles.

The analysis of small extracellular vesicles (sEVs) has shown clinical significance in early cancer diagnostics and considerable potential in prognostic assessment and therapeutic monitoring, offering possibilities for precise clinical intervention. Despite recent diagnostic progress based on blood-derived sEVs, the inability to specifically profile multiple parameters of sEVs proteins has hampered advancement in clinical applications. Herein, we report an approach to profile colorectal cancer (CRC)-derived sEVs by using multiaptamer-triggered rolling circle amplification (RCA) cascades.

Appendectomy, acute appendicitis, and gastrointestinal diseases: A Mendelian randomization study.

Background: Appendectomy is the standard treatment for acute appendicitis. However, the effects of appendectomy and acute appendicitis on gastrointestinal diseases are not fully understood.

Methods: Genetic variants associated with appendectomy and acute appendicitis at a genome-wide significance level (P ​< ​5.

A single-cell perspective on immunotherapy for pancreatic cancer: from microenvironment analysis to therapeutic strategy innovation.

Pancreatic cancer remains one of the most lethal malignancies, with conventional treatment options providing limited efficacy. Recent advancements in immunotherapy have offered new hope, yet the unique tumor microenvironment (TME) of pancreatic cancer poses significant challenges to its successful application. This review explores the transformative impact of single-cell technology on the understanding and treatment of pancreatic cancer.

Does snake-eye appearance affect the clinical outcome of ACDF in treating cervical myelopathy.

Purpose: To explore the impact of the snake-eye appearance (SEA) on the efficacy of anterior cervical discectomy and fusion (ACDF) in treating Degenerative Cervical Myelopathy (DCM).

Methods: A total of 316 Patients were divided into three groups, those with SEA were in the SEA group, and those with the absence of SEA were in the non-SEA group. Meanwhile, in the non-SEA group, patients with grade 1 or 2 increased signal intensity (ISI) on T2-weighted MRI were in the ISI group, and the remaining patients without ISI were in the non-ISI group.

A time series algorithm to predict surgery in neonatal necrotizing enterocolitis.

Background: Determining the optimal timing of surgical intervention for Neonatal necrotizing enterocolitis (NEC) poses significant challenges. This study develops a predictive model using the long short-term memory network (LSTM) with a focal loss (FL) to identify infants at risk of developing Bell IIB + NEC early and issue timely surgical warnings.

Methods: Data from 791 neonates diagnosed with NEC are gathered from the Neonatal Intensive Care Unit (NICU), encompassing 35 selected features.

Aging-Related CYP3A Functional Changes in Chinese Older Patients: New Findings from Model-Based Assessment of Amlodipine.

Aging-related alterations in hepatic enzyme activity, particularly of the CYP3A, significantly impact drug efficacy and safety in older adults, making it essential to understand how aging affects CYP function for optimal drug therapy. The exogenous probe substrate method, a minimally invasive approach to assess liver metabolic enzyme activity in vivo, is effective in studying these changes. Amlodipine being extensively metabolized (> 90%) in the liver, primarily via cytochrome P450 enzyme CYP3A was selected as a probe to investigate and quantify the factors affecting the aging-related changes of CYP3A in the Chinese older population.