Complete genome of the sp. f15 isolated from Daihai Lake in Inner Mongolia.

We isolated a strain of the genus from Daihai Lake in Inner Mongolia. The complete genome size is 2,614,554 bp, with a genomic DNA G + C content of 39.3%.

Sustainable MXene/Conductive Cellulose Heteroinks for 3D Printed High Areal Energy Density Micro-Supercapacitors and Self-Powered Integrated Systems.

The additive manufacturing of micro-supercapacitors (MSCs) with outstanding areal energy density and scalable integration remains challenging due to the incompatibility between printability and functionality of electronic ink. Here, a thixotropic MXene/conductive cellulose heteroink is formulated, eliminating the need for tedious processing and toxic organic additives, to construct MSCs with high areal energy density. Conductive cellulose with radially graded structure containing defect-rich graphitic shells not only inhibits MXene re-stacking through hydrogen-bonded 3D porous networks, but also establishes sp-carbon pathways for rapid electron transport.

Multi-Metagenome Analysis Unravels Community Collapse After Sampling and Hints the Cultivation Strategy of CPR Bacteria in Groundwater.

Groundwater harbors phylogenetically diverse Candidate Phyla Radiation (CPR) bacteria, representing an ideal ecosystem for studying this microbial dark matter. However, no CPR strains have been successfully isolated from groundwater, severely limiting further research. This study employed a multi-metagenome approach, integrating time-resolved sampling, antibiotic/nutrient interventions, and microbial correlation networks to unravel CPR ecological roles in groundwater and provide insights into their subsequent cultivation.

Injectable Double-Network Hydrogel-Based Three-Dimensional Cell Culture Systems for Regenerating Dental Pulp.

The regeneration of dental pulp tissue is very important, but difficult, in dentistry. The biocompatibility, water content, and viscoelastic properties of pulp-like tissue must be optimized to achieve the efficient transfer of metabolites and nutrients, a suitable degradation rate, distribution of encapsulated cells, injectability, and gelation under physiological conditions. As promising materials for pulp regeneration, hydrogel scaffolds have been produced to simulate the extracellular matrix and transmit signaling molecules.

Orrella daihaiensis sp. nov., a bacterium isolated from Daihai Lake in Inner Mongolia.

A novel aerobic, Gram-staining-negative, non-motile, short-rod-shaped strain, designated f23, was obtained from Daihai Lake, Inner Mongolia, Republic of China. 16S rRNA gene sequences analysis showed that f23 belongs to the genus Orrella and is most closely related to Orrella marina H-Z20 with 98.35% sequence similarity.

[Determination Methods of 2019 Novel Coronavirus (SARS-CoV-2)].

This study summarized the determination methods and principles of 2019 novel coronavirus(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2). The advantages and limitations of several methods was compared, which can provide a basis for the selection of 2019 novel coronavirus clinical diagnosis methods.

An injectable double-network hydrogel for the co-culture of vascular endothelial cells and bone marrow mesenchymal stem cells for simultaneously enhancing vascularization and osteogenesis.

The achievement of rapid vascularization in large implanted constructs is a major challenge in the field of bone tissue engineering. Although co-culture of bone-forming cells and vascular endothelial cells (VECs) has been expected to be a way of promoting vascularization during bone formation with a scaffold, there is a lack of detailed knowledge about the direct interactions between two types of stem cells in a three-dimensional (3D) extracellular matrix (ECM). Herein, we report on the use of an injectable cytocompatible double-network (DN) hydrogel to encapsulate, co-culture and subsequently stimulate the angiogenic/osteogenic differentiation of VECs and the human bone marrow mesenchymal stem cells (BM-MSCs), which demonstrates that the direct co-cultured system enables simultaneous enhancement of vascularization and osteogenesis by providing 3D cell-cell communication.

Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.

N-terminal acetylation (Nt-acetylation), carried out by N-terminal acetyltransferases (NATs), is a conserved and primary modification of nascent peptide chains. Naa60 (also named NatF) is a recently identified NAT found only in multicellular eukaryotes. This protein was shown to locate on the Golgi apparatus and mainly catalyze the Nt-acetylation of transmembrane proteins, and it also harbors lysine N(ε)-acetyltransferase (KAT) activity to catalyze the acetylation of lysine ε-amine.

[Influence of auricular point sticking on incidence of nausea and vomiting and analgesia effect after gynecological laparoscopy].

Objective: To observe the influence of auricular point sticking on incidence of nausea and vomiting and analgesia effect after gynecological laparoscopy, and provide evidence for clinical application of auricular point sticking.

Methods: One hundred and twenty cases of selective gynecological laparoscopy under general anesthesia were randomly divided into an auricular point sticking group and a placebo group, 60 cases in each group. In the auricular point sticking group, the auricular point sticking with vaccaria seeds was applied at Shenmen (TF 4), Wei (CO 4) and Jiaogan (AH 6a) before the operation and 1, 5, 9, 23 h after the operation, which were pressed 5 min each point each time.

Chemical and structural changes in preserved white egg during pickled by vacuum technology.

The aim of the present study was to investigate the chemical and structural changes in preserved white egg during pickled by vacuum technology. In order to evaluate the changes of preserved egg white during its salted period, sulfhydryl group, disulfide bond contents, and surface hydrophobicity were measured. Fourier transformation infrared spectroscopy and circular dichroism analysis were performed by considering changes of the secondary structural elements, the protein components of egg white was also studied in more detail by electrophoresis.