Self-Healing, Hemostatic, and Antioxidant Chitosan Hydrogel Loaded with Sophora Japonica Carbon Dots and ZnO NPs for Wound Healing.

This study presents a novel approach for preparing zinc oxide-sophora japonica carbon dots nanocomplexes via hydrothermal synthesis and co-precipitation, using Citric Acid (CA) as a green cross-linking agent and 3% chitosan (CS) as the raw material for hydrogel preparation. The zinc oxide-sophora flower carbon dots nanocomplexes are used as a model for hydrogel carrier formulation. Rat dorsal skin is selected as a model to evaluate the effect of the nanocomplex on wound healing.

Synthesis of Isosteviol derivatives as potential anticancer agents, especially for ovarian Cancer: In vitro cytotoxicity, cell cycle arrest, network pharmacology and molecular docking study.

Isosteviol is a tetracyclic diterpenoid from the hydrolysis of steviosidic acid, it exhibits a moderate inhibitory impact on tumor proliferation across various cancer types. Herein, we improved antitumor efficacy of isosteviol by modifying its reactive groups at C-16 and C-19 positions. A series of isosteviol derivatives 2-17, were synthesized and characterized.

Anti-inflammatory and antioxidant effects of haematococcus carbon dots in ulcerative colitis: A nanoparticle-based approach.

Ulcerative colitis (UC) is a chronic, non-specific inflammatory disease affecting the colon and rectum, classified as a type of inflammatory bowel disease (IBD). This study aimed to evaluate the therapeutic effects of Haematococcus carbon dots (HP-CDs) on dextran sulfate sodium (DSS)-induced ulcerative colitis in mice. HP-CDs were synthesized from Haematococcus pluvialis (HP) using a hydrothermal method involving Rhodococcus amphitrite.

Nanochannel confined graphene quantum dots/platinum nanoparticles boosts electrochemiluminescence of luminal-O system for sensitive immunoassay.

Sensitive detection of tumor biomarkers is of great significance for early cancer diagnosis, treatment evaluation, and recurrence monitoring. Development of convenient electrochemiluminescence (ECL) immunosensor using dissolved oxygen (O) as an endogenous co-reactant of luminol combined with efficient nanocatalysts to boost ECL signal in neutral media is highly desirable. Herein, sensitive detection of tumor biomarker using ECL of luminal-O enhanced by confinement of nitrogen-doped graphene quantum dots (N-GQDs) and platinum nanoparticles (PtNPs) on nanochannel array was demonstrated.

A Radical Mediator Layer on Composite Solid Polymer Electrolyte for Uniform Lithium Deposition.

Uneven diffusion and gradual accumulation of lithium under electric fields lead to the formation of lithium dendrite, which impedes the practical applications of all-solid-state lithium metal batteries. To achieve even deposition of Li, a free radical polymer (PTMA), poly (2,2,6,6-tetramethylpiperidinyloxy meth-acrylate), serving as Li transport and deposition mediator layer in Polyethylene oxide-LiLaZrO (PEO-LLZO) composite solid polymer electrolytes is employed. During the transporting process, Li is anchored by the O site of PTMA, hopping along PTMA chains until deposited onto the Li metal anode.

Preparation, characterization, and stability of chitosan-tremella polysaccharide layer-by-layer encapsulated astaxanthin nanoemulsion delivery system.

In this study, a layer-by-layer (LBL) encapsulated astaxanthin (Ast) nanoemulsion delivery system based on chitosan (CS) and tremella polysaccharide (TP) was successfully developed. The system constructed an Ast-CS-TP emulsion with high encapsulation efficiency and an excellent stability profile by utilizing the opposite charge properties of CS and TP. This study evaluated the effects of different stresses (including temperature, salt addition, pH, UV irradiation, and centrifugal force) on the emulsion's stability.

Direct and Sensitive Electrochemical Determination of Total Antioxidant Capacity in Foods Using Nanochannel-Based Enrichment of Redox Probes.

Simple and sensitive determination of total antioxidant capacity (TAC) in food samples is highly desirable. In this work, an electrochemical platform was established based on a silica nanochannel film (SNF)-modified electrode, facilitating fast and highly sensitive analysis of TAC in colored food samples. SNF was grown on low-cost and readily available tin indium oxide (ITO) electrode.

Bletilla striata carbon dots with alleviating effect of DSS-induced ulcerative colitis.

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) that significantly affected quality of life for patients. In this study, carbon dots based on Bletilla striata (BS-CDs) were synthesized by hydrothermal method and characterized by optical property analysis. In addition, the study measured the potential effect of BS-CDs on colonic histopathology and inflammation in dextran sulfate sodium (DSS)-induced ulcerative colitis.

antioxidant activity of the polysaccharide from and its structural characterisation.

A new polysaccharide (AAP) was extracted from by water extraction and alcohol precipitation. The antioxidant activity showed that AAP had a good scavenging effect on ABTS free radicals. Then AAP was purified by DEAE-52 ion exchange chromatography to obtain the purified component pAAP.

Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen.

Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is of great significance in cancer screening, early diagnosis, and postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through in situ growth of platinum nanoparticles (PtNPs) in a nanochannel array, which can achieve highly sensitive detection of the tumor marker carcinoembryonic antigen (CEA). An inexpensive and readily available indium tin oxide (ITO) glass electrode was used as the supporting electrode, and a layer of amino-functionalized vertically ordered mesoporous silica film (NH-VMSF) was grown on its surface using an electrochemically assisted self-assembly method (EASA).

N-Doped Graphene Quantum Dots Confined within Silica Nanochannels for Enhanced Electrochemical Detection of Doxorubicin.

Herein, we describe a fast and highly sensitive electrochemical sensor for doxorubicin (DOX) detection based on the indium tin oxide (ITO) modified with a binary material consisting of vertically-ordered mesoporous silica films (VMSFs) and N-doped graphene quantum dots (NGQDs). VMSFs, with high permeability and efficient molecular transport capacity, is attached to the ITO electrode via a rapid and controllable electrochemical method, which can serve as a solid template for the confinement of numerous NGQDs through facile electrophoresis. By virtue of the excellent charge transfer capacity, π-π and electrostatic preconcentration effects of NGQDs, as well as the electrostatic enrichment ability of VMSF, the presented NGQDs@VMSF/ITO shows amplified electrochemical signal towards DOX with a positive charge, resulting in good analytical performance in terms of a wide linear range (5 nM~0.

Disposable Amperometric Label-Free Immunosensor on Chitosan-Graphene-Modified Patterned ITO Electrodes for Prostate Specific Antigen.

A facile and highly sensitive determination of prostate-specific antigen (PSA) is of great significance for the early diagnosis, monitoring and prognosis of prostate cancer. In this work, a disposable and label-free electrochemical immunosensing platform was demonstrated based on chitosan-graphene-modified indium tin oxide (ITO) electrode, which enables sensitive amperometric determination of PSA. Chitosan (CS) modified reduced graphene oxide (rGO) nanocomposite (CS-rGO) was easily synthesized by the chemical reduction of graphene oxide (GO) using CS as a dispersant and biofunctionalizing agent.

Preparation, characterization, and performance evaluation of UiO-66 analogues as stationary phase in HPLC for the separation of substituted benzenes and polycyclic aromatic hydrocarbons.

UiO-66 analogues are good candidates as stationary phase in HPLC because of their chemical/thermal stability, large surface area, and two cage structures. Here, two UiO-66 analogues, UiO-66-NH2 and UiO-67, were synthesized and used as stationary phase in HPLC to evaluate their performance in the separation of substituted benzenes (SBs) and polycyclic aromatic hydrocarbons (PAHs). The results showed that SBs could be well separated on UiO-66-NH2 column but not on UiO-67 column.

Effects of soil properties on copper toxicity to earthworm Eisenia fetida in 15 Chinese soils.

The bioavailability and toxicity of metals in soil are influenced by a variety of soil properties, and this principle should be recognized in establishing soil environmental quality criteria. In the present study, the uptake and toxicity of Cu to the earthworm Eisenia fetida in 15 Chinese soils with various soil properties were investigated, and regression models for predicting Cu toxicity across soils were developed. The results showed that earthworm survival and body weight change were less sensitive to Cu than earthworm cocoon production.

Embedment of Ag(I)-organic frameworks into silica gels for microextraction of polybrominated diphenyl ethers in soils.

Three Ag(I)-organic frameworks, [Ag5(pydc)2(CN)]n, {[Ag4(pydc)2]CH3CN}n, and [Ag(4,4'-bpy)NO3]n, were synthesized and embedded into silica gels to form metal-organic-framework (MOF)-embedded gels for the microextraction of polybrominated diphenyl ethers (PBDEs) in soils. Despite the great differences in the structures of the organic ligands, all three Ag(I)-organic frameworks were found to effectively accumulate and concentrate PBDEs from sample solutions prepared with contaminated soil and purified water, indicating the important roles of Ag centers in PBDE extraction. Under the optimal experimental conditions (MOF mass, water volume, temperature, extraction time, and back-extraction time) for PBDE extraction from sample solutions, the detection limits of seven PBDEs (BDE-28, 47, 99, 100, 153, 154, and 183) ranged from 0.

Effects of a novel marine natural product: pyrano indolone alkaloid fibrinolytic compound on thrombolysis and hemorrhagic activities in vitro and in vivo.

Fungi fibrinolytic compound 1 (FGFC1) is a novel marine natural product as a low-weight fibrinolytic pyranoindole molecule, whose thrombolytic effects were evaluated on FITC-fibrin (Fluorescein isothiocyanate, FITC) degradation methods in vitro and on acute pulmonary thromboembolism animal model in vivo. We determined the FGFC1 induced thrombolysis that stems from its fibrin(ogen)olytic activities as measured by fibrin(ogen) degradation products (FDPs) experiment, acute pulmonary thromboembolism animal model experiment, and euglobulin lysis assay. In vitro, measurement of FITC-fibrin degradation revealed that fibrin hydrolysis occurred in a concentration-dependent manner of FGFC1 from 5 to 25 μ mol/L.

Separations of substituted benzenes and polycyclic aromatic hydrocarbons using normal- and reverse-phase high performance liquid chromatography with UiO-66 as the stationary phase.

Metal-organic frameworks (MOFs) have great potential for applications in chromatography due to their highly tailorable porous structures and unique properties. In this work, the stable MOF UiO-66 was evaluated as both a normal-phase (NP-) and a reverse-phase (RP-) stationary phase in the high performance liquid chromatography (HPLC) to separate substituted benzenes (SBs) and polycyclic aromatic hydrocarbons (PAHs). It was found that the mobile phase composition has a significant effect on the HPLC separation.

Pharmacokinetics and tissue distribution of a novel marine fibrinolytic compound in Wistar rat following intravenous administrations.

We investigated a novel marine fibrinolytic compound for use in thrombolytic therapy. Pharmacokinetics and the tissue distribution of this novel marine fibrinolytic compound, FGFC1(2) (fungi fibrinolytic compound 1), were investigated in Wistar rats after intravenous (IV) bolus administration of two concentrations (10 and 20mg/kg). Plasma FGFC1 and tissue extracts were measured using HPLC with UV detection.

[Response surface methodology to optimize marine microbe culture for producing fungi fibrinolytic compound].

Response surface methodology was applied to optimize the fermentation conditions of FGFC1 (Fungi fibrinolytic compound 1). On the basis of single factor tests, response surface analysis was designed by Design-Expert, and the effects of culture time, ornithine hydrochloride addition and culture temperature on the yield of FGFC1 were studied, the predicted value and measured value were also contrasted. The results show the optimal culture conditions as follows: the culture time is 7 d, ornithine hydrochloride addition is 0.

[Human health risk-based environmental criteria for soil: a comparative study between countries and implication for China].

Since the 1990s, most developed countries have issued the risk-based environmental standards or criteria for soil, as well as corresponding technical background documents, this provides methodological references for developing soil environmental standards and criteria in developing countries. However, because of the variability in legislation frameworks, derivation procedures of criteria, environmental conditions and climates, soil types, and habits and customs among countries, there are great differences in the names, roles and values of soil standards and criteria. This paper presents the scientific basis and fundamental features of soil environmental criteria.

The protection of polysaccharide from the Brown Seaweed Sargassum graminifolium against ethylene glycol-induced mitochondrial damage.

The aim of the present study is to evaluate the protective effect of polysaccharide from the Brown Seaweed Sargassum graminifolium (SGP) on ethylene glycol-induced kidney damage and the mechanism of SGP-mediated protection. Mitochondrial lipid peroxidation, mitochondrial swelling, the activity of succinate dehydrogenase (SDH), ATPases and mitochondrial antioxidant enzymes was observed in hyperoxaluric rats. Administration of SGP (25, 100 and 400 mg·kg-1, intragastrically) increased the activities of antioxidant enzymes, SDH and Na+/K+-ATPases, Ca2+-ATPases, Mg2+-ATPases, also decreased mitochondrial lipid peroxidation and mitochondrial swelling.

Application of portable gas chromatography-photo ionization detector combined with headspace sampling for field analysis of benzene, toluene, ethylbenzene, and xylene in soils.

A method based on headspace (HS) sampling coupling with portable gas chromatography (GC) with photo ionization detector (PID) was developed for rapid determination of benzene, toluene, ethylbenzene, and xylenes (BTEX) in soils. Optimal conditions for HS gas sampling procedure were determined, and the influence of soil organic matter on the recovery of BTEX from soil was investigated using five representative Chinese soils. The results showed that the HS-portable-GC-PID method could be effectively operated at ambient temperature, and the addition of 15 ml of saturated NaCl solution in a 40-ml sampling vial and 60 s of shaking time for sample solution were optimum for the HS gas sampling procedure.

Antioxidant properties of polysaccharide from the brown seaweed Sargassum graminifolium (Turn.), and its effects on calcium oxalate crystallization.

We investigated the effects of polysaccharides from the brown seaweed Sargassum graminifolium (Turn.) (SGP) on calcium oxalate crystallization, and determined its antioxidant activities. To examine the effects of SGP on calcium oxalate crystallization, we monitored nucleation and aggregation of calcium oxalate monohydrate crystals, using trisodium citrate as a positive control.