AlphaFold-Assisted Design and Synthesis of V-Type Amidazoles as Chitin Synthase Inhibitors against .

Chitin synthase serves as a promising target for developing eco-friendly insecticides. Nevertheless, the lack of structural insights into insect chitin synthase has impeded the rational design of specific inhibitors. Herein, we utilized AlphaFold to predict the structure of Chs1.

sp. nov., isolated from the coal samples in Shanxi Province of China.

Two Gram-negative, oxidase-positive, catalase-positive, non-spore-forming, motile, short-rod strains (G13 and G34) were isolated from coal block samples. Phylogenetic analyses based on 16S rRNA genes and 718 core genes indicated that both strains belonged to the genus , with the closest relative being AIMA4 as their closest relative. Average nucleotide identity and digital DNA-DNA hybridization values were 79.

sp. nov. and sp. nov., two -cryptoxanthin-producing bacteria isolated from intestinal contents of .

Four Gram-stain-positive, facultative anaerobic, yellow-pigmented and short rod-shaped strains, designated zg-Y625, zg-Y843, zg-Y1090 and zg-Y1211, were isolated from the intestinal contents of in Qinghai Province, PR China. Strains zg-Y625 and zg-Y1090 showed the highest 16S rRNA gene sequence similarities of 99.7% and 99.

sp. nov. and sp. nov. isolated from the faeces of in the Qinghai-Tibet Plateau of China.

Four novel, Gram-stain-positive, catalase-positive, oxidase-negative, aerobic and non-motile strains, designated MJ206, MJ278, MJ170 and MJ173, were isolated from the faeces of in the Qinghai-Tibet Plateau of China. A comparative analysis of 16S rRNA gene sequences revealed that strain pairs MJ206 and MJ278, as well as MJ170 and MJ173, belong to the genus . Strain pair MJ206/MJ278 is most closely related to '' ZLJ0423 (98.

sp. nov., and sp. nov., two new members of the genus isolated from plateau pika ().

Four anaerobic, Gram-stain-positive, non-motile, non-sporulating rod-shaped bacterial strains (R7, R21, R22 and R25) were isolated from the intestinal contents of plateau pika () collected from the Qinghai-Tibet Plateau, PR China. The four isolates grew at between 25 and 42 °C (optimally at 35-37 °C), and with 0.3-3.

alleviates hyperglycemia and regulates gut microbiota and metabolic profiles in mice.

Unlabelled: is the dominant species of the genus in the gut, which is genomically heterogeneous and difficult to isolate; hence, scarce research was carried out for this species. This study aimed to investigate the effect of on hyperglycemia. Thirty-nine strains were isolated from healthy individuals, and three strains (HF2123, HF1478, and HF2130) that had the highest glucose consumption were selected to evaluate the effects of supplementation on hyperglycemia.

Taxonomic descriptions of sp. nov., sp. nov. and sp. nov.

Six Gram-stain-positive, facultative anaerobic, nonmotile and rod-shaped strains, designated zg-Y50, zg-Y1362, zg-Y1379, zg-Y869, zg-629 and zg-Y636, were isolated from the intestinal contents of in Qinghai Province, PR China. Strains zg-Y50, zg-Y1379 and zg-629 exhibited the highest 16S rRNA gene sequence similarities of 99.2, 98.

, gen. nov., sp. nov., a novel bacterial species of the family that could produce flexirubin type pigments.

Two Gram-stain-negative, non-spore-forming, rod-shaped, and obligately aerobic bacteria, designated strains CX-624 and cx-311, were isolated from soil samples in Qinghai Province, China. The two strains grew best at 28 °C on the plate with Tryptone soya agar (TSA). Cells formed circular, convex, translucent, smooth, and orange colonies with approximately 1.

sp. nov. and sp. nov., isolated from the respiratory tracts of marmots.

Four Gram-stain-negative, oxidase-positive, non-motile, cocci-shaped bacteria strains (ZJ106, ZJ104, ZJ785 and ZJ930) were isolated from marmot respiratory tracts. Phylogenetic analyses based on 16S rRNA genes, 53 ribosomal protein sequences and 441 core genes supported that all four strains belonged to the genus with close relatives 10022 and ATCC 51483. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were below the species-level thresholds (95-96 % for ANI, and 70 % for dDDH).

Species-level microbiota of ticks and fleas from in the Qinghai-Tibet Plateau.

Introduction: Ticks and fleas, as blood-sucking arthropods, carry and transmit various zoonotic diseases. In the natural plague foci of China, monitoring of has been continuously conducted in and other host animals, whereas other pathogens carried by vectors are rarely concerned in the Qinghai-Tibet Plateau.

Methods: In this study, we investigated the microbiota of ticks and fleas sampling from in the Plateau, China by metataxonomics combined with metagenomic methods.

sp. nov., sp. nov. and sp. nov., three cellulolytic bacteria isolated from intestinal contents of .

Six facultative anaerobic, Gram-stain-positive, oxidase-negative, rod-shaped bacteria (strains zg-B89, zg-B12, zg-Y338, zg-Y138, zg-Y908 and zg-Y766), were isolated from the intestinal contents of in Qinghai Province, PR China. The 16S rRNA gene sequence analysis showed that zg-B89 showed highest similarity to NBRC 101100 (99.5 %), zg-Y338 to DSM 20118 (98.

sp. nov., sp. nov. and sp. nov., isolated from faeces from Qinghai-Tibet Plateau.

Six aerobic or facultative anaerobic, motile, Gram-stain-positive, catalase-positive and oxidase-negative strains (zg-Y453, zg-Y324, zg-Y462, zg-Y411, zg-Y809 and zg-Y786) were isolated from different faecal samples of from the Qinghai-Tibet Plateau. Pale yellow, round, raised and moist colonies appeared 48 h after incubation at 28 °C on brain-heart infusion plates supplemented with 5 % defibrinated sheep blood. According to the 16S rRNA gene sequence alignment, two strain pairs (zg-Y453/zg-Y324 and zg-Y462/zg-Y411) shared the highest similarities to (99.

sp. nov., sp. nov., and sp. nov., isolated from soil of the Qinghai-Tibetan Plateau.

Six novel bacterial strains, designated CY22, CY357, LJ419, LJ53, CY399 and CY107 were isolated from soil samples collected from the Qinghai-Tibetan Plateau, PR China. Cells were aerobic, rod-shaped, yellow-pigmented, catalase- and oxidase-positive, Gram-stain-negative, non-motile and non-spore-forming. All strains were psychrotolerant and could grow at 0 °C.

sp. nov. and sp. nov. isolated from .

Four Gram-stain-positive, facultatively anaerobic, non-motile, non-spore-forming and rod-shaped bacteria (lx-72, lx-45, ZJ784 and ZJ955) were isolated from the respiratory tract or faeces of marmot () from the Qinghai-Tibet Plateau in China. Analysis of the 16S rRNA gene sequences showed that all strains belong to the genus and are more related to CCUG 64069 (95.1-97.

Immunoprotective Analysis of the NFA49590 Protein from IFM 10152 Demonstrates Its Potential as a Vaccine Candidate.

Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to the investigation of a vaccine approach to prevent Nocardia infections. However, studies on the protective proteins of Nocardia have not yet been carried out.

sp. nov., sp. nov. and sp. nov., isolated from the intestinal contents of .

Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157/zg-ZUI40, zg-ZUI222/zg-ZUI199 and zg-ZUI188/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C.

Discovery of New Anti-MRSA Agents Based on Phenoxyethanol and Its Mechanism.

Methicillin-resistant (MRSA) poses a severe threat to public health and safety. The discovery and development of novel anti-MRSA drugs with a new mode of action are a challenge. In this study, a class of novel aryloxyethyl propiolates and their homologues as anti-MRSA agents have been designed and synthesized based on phenoxyethanol, of which compound showed high inhibitory activity against MRSA with an MIC of 0.

sp. nov., sp. nov. and sp. nov., isolated from the Qinghai-Tibet Plateau.

Six Gram-stain-positive, aerobic and irregular-rod-shaped actinobacteria (ZJ1313, ZJ1307, MC1495, Y192, 603 and X2025) were isolated from the Qinghai-Tibet Plateau of China and were characterized using a polyphasic taxonomic method. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the six new strains formed three distinct clusters within the genus , and strains ZJ1313 and ZJ1307 were most closely related to JCM 31492 (16S rRNA gene sequence similarity, 98.0 %), MC1495 and Y192 to 78 (98.

Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome.

Two Gram-stain-positive, catalase-negative, non-spore-forming, cocci-shaped strains (dk850 and JY899) were isolated from the feces of Equus kiang in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based phylogenetic analyses showed that strains dk850 and JY899 belong to the genus Flaviflexus, closest to F. salsibiostraticola KCTC 33148, F.

sp. nov. and sp. nov., two new rapidly growing mycobacterial species.

Four bacterial strains (LJ126/S18 and Z-34/S20) recovered from faecal samples of Tibetan antelopes on the Qinghai-Tibet Plateau of China were analysed using a polyphasic approach. All four isolates were aerobic, short rod-shaped, non-motile, Gram-stain-positive, acid-fast and fast-growing. Phylogenetic analyses based upon 16S rRNA and whole-genome sequences showed that the two pair of strains formed two distinct branches within the evolutionary radiation of the genus .

sp. nov. isolated from trachea of , sp. nov. and sp. nov. from human faeces.

Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320/zg-336, zg-917/zg-910 and zg-913/zg-915) isolated from animal tissues and human faeces were found to belong to the genus based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320/zg-336 had the highest 16S rRNA gene similarity to DSM 44353 (97.51 %), zg-917/zg-910 to DSM 44184 (98.