Lab-on-Robot: Unmanned Mass Spectrometry Robot for Direct Sample Analysis in Hazardous and Radioactive Environments.

Onsite, safe, and reliable mass spectrometry (MS) analysis of hazardous and radioactive samples plays a crucial role in timely chemical emergency management and response in real environments. The current study reports the development of a smart MS robot by integrating miniature MS, quadruped robot, switchable robotic arm sampler, and direct ionization for remote-controlled chemical analysis of complex samples in inaccessible hazardous and radioactive environments. High automation and excellent analytical performance have been achieved in the real-time analysis of volatile toxic substances in air and onsite detection of explosive particles in air aerosols.

Transposable elements shape the landscape of heterozygous structural variation in a bird genome.

Avian genomes exhibit compact organization and remarkable chromosomal stability. However, the extent and mechanisms by which structural variation in avian genomes differ from those in other vertebrate lineages are poorly explored. This study generated a diploid genome assembly for the golden pheasant ( ), a species distinguished by the vibrant plumage of males.

A review of uranium (U) elemental detection methods.

With the increasing demand for energy, nuclear energy has been developing rapidly. The quantitative detection and qualitative identification of uranium (U) are of great significance for the comprehensive and efficient use of U resources and the control of nuclear and radioactive substances. In this study, the detection of U is divided into liquid sample detection, solid sample detection, gas sample detection, and industrial detection from the perspectives of the sample state and detection environment.

Phosphorus Metabolism-Related Genes Serve as Novel Biomarkers for Predicting Prognosis in Bladder Cancer: A Bioinformatics Analysis.

Background: Phosphorus metabolism might be associated with tumor initiation and progression. We aimed to screen out the phosphorus metabolism genes related to bladder cancer and construct a clinical prognosis model.

Methods: The dataset used for the analysis was obtained from TCGA database.

Multiomics reveals the ameliorating effect and underlying mechanism of aqueous extracts of polygonatum sibiricum rhizome on obesity and liver fat accumulation in high-fat diet-fed mice.

Background: Polygonatum sibiricum polysaccharides protect against obesity and NAFLD. However, the potential effects of PS rhizome aqueous extracts (PSRwe) on adiposity and hepatic lipid accumulation remains unexplored.

Purpose: Elucidating the impact and underlying mechanism of PSRwe on HFD-induced obesity and liver fat depostition.

In-depth multiomic characterization of the effects of obesity in high-fat diet-fed mice.

High-fat diet (HFD)-fed mice have been widely used in the clinical investigation of obesity. However, the long-term effect of HFD on gut microbiota and metabolites, plasma and liver metabolomics, colonic and liver transcriptomics remain largely unknown. In this study, 6-week-old C57BL/6J male mice fed with HFD for 14 weeks showed increased obesity-related indexes including alanine aminotransferase, aspartate aminotransferase, total cholesterol, total triglyceride, free fatty acids, lipopolysaccharides, IL-6, and TNFα.

A PD-L1xCD3 bispecific nanobody as a novel T-cell engager in treating PD-L1 overexpression melanoma.

The development of Immune checkpoint blockade(ICB) therapy and BRAF- and MEK-targeted therapies has reshaped the survival outcomes of the patients with advanced melanoma. PD-1/PD-L1 blockade was an approved strategy in melanoma treatment. Here we design a PD-L1 xCD3 nanobody as a novel bispecific T cell engager (BiTE) in treating PD-L1 overexpression melanoma.

Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p.

Warburg effect plays a crucial role in bladder cancer (Bca) development. However, the mechanism by which glycolysis is involved in Bca remains poorly understood. CircRNAs commonly play a regulatory role in tumor progression.

A high-quality genome assembly highlights the evolutionary history of the great bustard (Otis tarda, Otidiformes).

Conservation genomics often relies on non-invasive methods to obtain DNA fragments which limit the power of multi-omic analyses for threatened species. Here, we report multi-omic analyses based on a well-preserved great bustard individual (Otis tarda, Otidiformes) that was found dead in the mountainous region in Gansu, China. We generate a near-complete genome assembly containing only 18 gaps scattering in 8 out of the 40 assembled chromosomes.

Identification of Signature Genes Associated With Invasiveness and the Construction of a Prognostic Model That Predicts the Overall Survival of Bladder Cancer.

Bladder cancer has become the tenth most diagnosed cancer worldwide. The prognosis has been shown to differ between non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). We aimed to identify signature genes that are associated with the invasiveness and survival of bladder cancer and to identify potential treatments.

Determination of trace lithium in uranium compounds by adsorption on activated alumina using a micro-column method.

A novel method using a micro-column packed with active alumina as solid phase was proposed for separation of trace lithium from uranium compounds prior to determination. The method is based on a preliminary chromatographic separation of the total amount of uranium. This separation involves passing the solution containing sodium carbonate through active alumina and then eluting the trace lithium retained by the solid phase with a solution of sulfuric acid.

[Effect of aminoguanidine on hemodynamics and nitrogen monoxide in hemorrhagic shock in rabbit].

Objective: To explore the effect of aminoguanidine (AG) on hemodynamics and nitric oxide (NO) in hemorrhagic shock in rabbit.

Methods: Shock was produced in twenty rabbits by femoral artery blood letting, and they were randomly divided into two groups with treatment with saline (control group) or AG (each n=10). Pclab system and nitric acid reductase methods were performed to detect hemodynamics parameters and NO levels at preshock, postshock (30 minutes and 150 minutes) and 30 minutes and 180 minutes after resuscitation respectively.