Targeted metabolomic and transcriptomic reveal the regulatory network of ultrasound on polyphenol biosynthesis in tender coconut flesh during storage.

Coconut flesh, the solid endosperm, of coconut, which is rich in fat, protein and polyphenols. To investigate the impact of ultrasound treatment on the biosynthesis of polyphenols in tender coconut flesh during storage, the targeted metabolomic and transcriptomic analyses were employed. A total of 36 phenolic compounds were identified, of which catechin, epicatechin, gossypol and vanillic acid were the most abundant ones in 'Hainan Tall' coconut flesh.

Large-scale genomic deletion in activates immune responses and confers resistance to rice bacterial blight.

Lesion mimic mutants (LMMs) are invaluable for uncovering the molecular mechanisms of programmed cell death (PCD) and plant immunity and identifying more LMMs expands our understanding of these complex processes. In this study, we characterized a novel rice LMM, , identified through heavy-ion beam irradiation. The mutant exhibits reddish-brown lesions from the tillering stage, reduced plant height, grain size, and fertility.

Bionic Multilevel Composite Structures Design in Ag-CuO Contacts for Synergistically Enhancing Erosion and Impact Resistance.

The synergistic enhancement effect among multilevel composite structures is essential for improving the overall performance of contacts. Notably, natural bamboo demonstrates exceptional mechanical properties through the coupling effect of its structure. By reforming the microstructure of bamboo, an Ag-CuO contact, consisting of the Ag-rich regions, the CuO chain-like skeleton regions, and the CuO barrier layers, was inversely designed and controllably fabricated.

Profiles of oral microbiota and metabolites in periodontitis and benign prostatic hyperplasia patients: a pilot study.

Emerging evidence suggests a potential link between periodontitis and benign prostatic hyperplasia (BPH). We have demonstrated that experimental periodontitis and the periodontal pathogen exacerbate the progression of BPH. In this study, we focused on the characteristics and alterations of the oral microbiome and metabolites in patients with BPH and those with both BPH and periodontitis.

MB-MSTFNet: A Multi-Band Spatio-Temporal Attention Network for EEG Sensor-Based Emotion Recognition.

Emotion analysis based on electroencephalogram (EEG) sensors is pivotal for human-machine interaction yet faces key challenges in spatio-temporal feature fusion and cross-band and brain-region integration from multi-channel sensor-derived signals. This paper proposes MB-MSTFNet, a novel framework for EEG emotion recognition. The model constructs a 3D tensor to encode band-space-time correlations of sensor data, explicitly modeling frequency-domain dynamics and spatial distributions of EEG sensors across brain regions.

Dysregulation of adipocytokines via the hsa-miR-548ay-3p/PPARγ signaling pathway leads to cardiac fibrosis.

Cardiac fibrosis is a chief cause of atrial fibrillation (AF), yet its mechanism is not fully understood. Our previous study found that fibrosis-related adipocytokines were dysregulated in epicardial adipose tissue (EAT) from patients with AF. The present study explored the factors inducing adipocytokine dysregulation and the mechanism by which this dysregulation leads to cardiac fibrosis.

Efficacy and Safety of 10-Day Minocycline Twice Daily in Bismuth-Containing Quadruple Therapy as the First-Line Treatment of Infection: A Prospective Single-Arm Study.

Background: Tetracycline has limited clinical application in treatment because of difficulty in obtaining and increased adverse reactions. As a semisynthetic tetracycline, minocycline has demonstrated good potential for eradicating infection. This study aimed to evaluate the efficacy and safety of 10-day minocycline-based quadruple therapy for first-line treatment.

Multimodal radiopathomics approach for predictions of prognosis and immunotherapy response in patients with GC: a multicohort retrospective study.

Background: Current TNM staging systems insufficiently predict individual prognosis and chemotherapy response in gastric cancer (GC). We aimed to develop and validate a radiopathomics signature (RPS) integrating computed tomography (CT) and pathological features to improve prognostic stratification and guide treatment decisions. Preliminary evaluation of immunotherapy response was also conducted.

A novel autologous material for the surgical treatment of Peyronie's disease: the outer plate of the prepuce.

Background: Peyronie's disease (PD), a connective tissue disease, is characterized by the formation of fibrous lesions or plaques in the tunica albuginea. Many PD patients rely on surgical treatment methods. This study aimed to investigate the feasibility of using the outer plate of the prepuce to repair the defect of the corpus cavernosum after plaque enucleation in patients with PD.

Joint analysis of whole-genome methylation and transcriptome in avian pullorum disease and validation of gene function.

Unlabelled: Pullorum disease caused by can negatively affect growth and egg-laying performance, resulting in significant economic losses in poultry farming. In this study, spleen tissues from SP-infected chickens (group P) and SP-uninfected chickens (group N) were analyzed for functional enrichment using whole-genome bisulfite and RNA sequencing. The function of the key gene was verified by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.

Clinical feasibility of AI Doctors: Evaluating the replacement potential of large language models in outpatient settings for central nervous system tumors.

Background And Objectives: The treatment of central nervous system (CNS) tumors is complex and resource-intensive, with higher mortality in underserved regions. Large language models (LLMs) show promise in medical support, but their real-world performance in CNS tumor outpatient care remains unclear. This study aims to assess the diagnostic and treatment capabilities of LLMs in bilingual clinical settings.

Regulatory mechanisms of O6-methylguanine methyltransferase expression in glioma cells.

O6-methylguanine methyltransferase (MGMT), a pivotal DNA repair enzyme, has its dysregulation playing a substantial role in gliomagenesis, the development of therapeutic resistance, and patient prognosis. This narrative review is designed to offer an all-encompassing overview of the intricate regulatory mechanisms that govern MGMT expression in glioma cells. We systematically investigate the diverse levels of regulation that impact MGMT expression in glioma.

Simplifying surgical criteria for early-stage cervical cancer: prognostic equivalence of total vs. radical hysterectomy in a retrospective SEER cohort analysis.

Background: The prognostic equivalence of total hysterectomy (TH) versus radical hysterectomy (RH) in early-stage cervical cancer (IA2-IB1) with tumor size ≤2 cm remains controversial, particularly regarding the necessity of lymphovascular space invasion (LVSI) assessment. This study evaluates survival outcomes under simplified criteria omitting LVSI and depth of invasion evaluation.

Materials And Methods: This retrospective cohort study analyzed 3,002 FIGO IA2-IB1 cervical cancer patients (tumors ≤2 cm) from the SEER database (2004-2019).

Salmonella enterica mediated epigenetic promotion of fibrosis is a novel factor in benign prostatic hyperplasia.

Background: Fibrosis constitutes a significant pathophysiological mechanism in the clinical progression of benign prostatic hyperplasia (BPH) and represents a contributing factor to the ineffectiveness of prevailing pharmacological treatments. Emerging evidence suggests a close association between microbial presence and the development of fibrosis. Nonetheless, the potential involvement of microbes within prostatic tissue in the pathogenesis of BPH and prostatic fibrosis, along with the underlying mechanisms, remains unexplored.

Grain-sized moxibustion activates dendritic cells to enhance the antitumor immunity of cancer vaccines.

Background: Moxibustion, a traditional Chinese medicine (TCM) therapy, employs thermal stimulation from the combustion of Artemisia argyi H.Lév. & Vaniot at acupoints to treat "deficiency-cold syndromes" (xuhan zheng), historically linked to immune dysfunction and chronic inflammation.

Giant Nonvolatile Multistate Resistance with Fully Magnetically Controlled van der Waals Multiferroic Tunnel Junctions.

Ferroelectric polarization switching in electrically controlled van der Waals multiferroic tunnel junctions (vdW-MFTJs) causes atomic migration, compromising device stability and fatigue resistance. Here, we propose fully magnetically controlled vdW-MFTJs based on a CrBr/MnPSe/CrBr vertical heterostructure, achieving ferroelectric polarization reversal without atomic migration. First-principles calculations reveal that integrating PtTe/alkali-metal (Li/Na/K)-doped/intercalated CrBr electrodes enables exceptional performance, with a maximum tunneling magnetoresistance (TMR) of 8.

Indirect Comparisons of Efficacy of Zanubrutinib Versus Orelabrutinib in Patients with R/R MCL: An Extended Follow-up Analysis.

Introduction: Our previous study has suggested a favorable progression-free survival (PFS) with zanubrutinib over orelabrutinib in patients with relapsed or refractory mantle cell lymphoma (R/R MCL). Here, we conducted an updated analysis to indirectly compare the long-term efficacy between zanubrutinib and orelabrutinib in patients with R/R MCL.

Methods: Individual patient data from the zanubrutinib study were adjusted to match the patient population profile of the orelabrutinib study.

Heat Stress Influences Immunity Through and Mediated Antigen Presentation in Chickens.

The objective of this study was to elucidate the immune system response to heat stress in chickens. In this study, mRNA-seq was conducted on the spleen and bursa of experimental chickens, six differentially expressed genes associated with immunity were present in the spleen following immunization. Following exposure to heat stress, 15 differentially expressed genes related to immune and heat shock proteins were identified.

LC-MS/MS-Based Determination and Optimization of Linoleic Acid Oxides in and Their Variation with Storage Time.

: Post-production storage plays a pivotal role in developing the characteristic flavor profile of , a traditional alcoholic beverage in China. While aging markers remain crucial for quality authentication, the identification of reliable metabolic indicators for chronological determination requires further exploration. : This study establishes a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) methodology for quantifying five linoleic acid-derived oxidative metabolites in : 9,12,13-trihydroxy-10(E)-octadecenoic acid (9,12,13-TriHOME), 9,10-Dihydroxy-12-octadecenoic acid (9,10-DiHOME), 9-oxo-(10E,12Z)-octadecadienoic acid (9-OxoODE), 9-hydroxy-(10E,12Z)-octadecadienoic acid (9-HODE) and 13-hydroxyoctadeca-(9Z,11E)-octadecadienoic acid (13-HODE).

Study on the collaborative protective mechanism of and against diabetic cardiomyopathy through the gut-heart axis.

The modification of gut microbiota has been linked to diabetic cardiomyopathy, yet the precise mechanisms through which gut microbes impact cardiac injury remain unclear. Our study concentrated on the gut microorganisms, the intestinal mucosal barrier, and the metabolic pathways involving glucose and lipids in mice afflicted with diabetic cardiomyopathy, while also investigating the cardioprotective properties of and . Using a db (Leptin receptor gene-deficient mouse) mouse model of diabetic cardiomyopathy, we observed that these mice exhibited a decline in the diversity of intestinal microbes, alterations in the abundance of diabetes-related microorganisms, a decrease in , an increase in , and an overall rise in intestinal microbial populations.

Comparative survival analysis of stage T1-T2N0M0 lung squamous cell carcinoma and adenocarcinoma using SEER data, and nomogram analysis for early-stage lung squamous cell carcinoma.

Background: Lung cancer is one of the most common malignant tumors worldwide. It is of great significance to conduct in-depth research on early lung cancer with a better prognosis. This study aimed to use the Surveillance, Epidemiology, and End Results (SEER) database to compare the clinicopathological characteristics and survival between early squamous cell carcinoma (SQCC) and adenocarcinoma (AC) under the same treatment model, and develop a nomogram for early lung SQCC.

Application of Environmental DNA for Assessing the Distribution and Biomass of Tsinlingensis in the Zhouzhi Heihe River.

In recent years, the population of has experienced a rapid decline due to habitat degradation caused by overfishing and dam construction. In the current study, water samples were collected from four tanks containing individuals with wet weights of 5.8 g, 12.

Discovery of the pharmacodynamic material basis of Danggui Buxue Decoction in the treatment of diabetic kidney disease based on lipidomics regulation.

Background: Danggui Buxue Decoction (DBD) is a formula used for treating diabetic kidney disease (DKD). However, the pharmacodynamic material basis of DBD in DKD therapy remains unclear, hindering its industrial development and innovation in drug formulations.

Purpose: Lipid metabolism disorder is a key pathological mechanism in DKD progression.

Intrinsic ion migration-induced susceptible two-dimensional phase-transition memristor with ultralow power consumption.

Two-dimensional (2D) phase-transition memristors have demonstrated transformative potential for neuromorphic computing, yet challenges like high power consumption, limited endurance, and crystal damage from external ion intercalation persist. Here, we introduce a novel 2D phase-transition memristor leveraging a paradigm-shifting mechanism by exploiting the ultrafast intrinsic Cu ion migration within CuS. This approach eliminates the need for external ion insertion, significantly reducing crystal damage and enabling exceptional cycling stability with over 400 DC cycles and 500 pulse cycles.

Progress and perspectives of metabolic biomarkers in blood sample for diabetic microvascular complications.

Background: Diabetes mellitus refers to a group of metabolic diseases characterized by chronic hyperglycemia due to multiple etiological factors. As the disease progresses, patients gradually develop microvascular complications, including diabetic nephropathy, diabetic retinopathy, and diabetic neuropathy. However, current clinical methods for detecting these microvascular complications are limitations, thus primary prevention and early diagnosis are of great importance.