Simultaneous determination of organotin pesticides by HPLC-ICP-MS and their sorption, desorption, and transformation in freshwater sediments.

In order to better assess their environmental risks, the sorption and degradation of triphenyltin hydroxide, azocyclotin and fenbutatin oxide were studied in two sediments under varying laboratory conditions in this study. An analytical method for simultaneous determination of the three organotins in environmental samples was firstly developed using high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The limit of detection and limit of quantification for standards ranged from 0.

Retinol and α-tocopherol in human milk and their relationship with dietary intake during lactation.

Human milk is considered as an optimal nutrition source for neonates, and vitamins A and E improve oxidative stress in offspring through breast milk. We aim to determine α-tocopherol and retinol contents in breast milk obtained over the course of lactation stages from mothers in three regions in China, and to investigate their correlation with maternal dietary intake during lactation. Human milk samples and 24 hour food records were obtained from mothers in Hangzhou (n = 34), Lanzhou (n = 34) and Beijing (n = 34) on day 1 (colostrum), day 14 (transitional milk) and day 42 (mature milk) postpartum.

Offspring caregivers' depression affected by intergenerational disagreements on preferred living arrangement for the elderly: A phenomena with Chinese characteristic.

Objective: This study aimed to explore whether the depression of offspring caregivers can be affected by the intergenerational disagreements on preferred living arrangements for the elderly, and the extent of this influence.

Methods: A total of 875 participants from five urban neighborhoods were investigated in a cross-sectional survey in Jinan, China. Depressive symptoms were assessed by the Self-rating Depression Scale (SDS).

Associations of ACE gene insertion/deletion polymorphism, ACE activity, and ACE mRNA expression with hypertension in a Chinese population.

Background: The present study was designed to explore the association of angiotensin converting enzyme (ACE) gene insertion/deletion (I/D, rs4646994) polymorphism, plasma ACE activity, and circulating ACE mRNA expression with essential hypertension (EH) in a Chinese population. In addition, a new detection method for circulating ACE mRNA expression was explored.

Methods: The research was approved by the ethics committee of Zhejiang Provincial Center for Disease Prevention and Control.

Unintended pregnancy and its correlates among female attendees of sexually transmitted disease clinics in Eastern China.

This study is to determine the prevalence of unintended pregnancy and its risk factors among the female attendees of sexually transmitted disease (STD) clinics in Zhejiang Province, China. A self-administered questionnaire survey of a cross-sectional design was administered to attendees at four STD clinics in 2007. Of the 313 female STD clinic attendees, 42.

The effects of booster vaccination on combined hepatitis A and hepatitis B vaccine in both anti-HBs and anti-HAV negative children 5-15 years after hepatitis B vaccine primary immunization.

In the present study, we investigated the changes in both anti-HAV lgG and anti-HBs lgG levels and compared the antibody seroconversion rates of different doses of combined hepatitis A and hepatitis B vaccine in children. Children who were vaccinated as infants with Hepatitis B vaccine were revaccinated at 5-15 y of age, then the antibody titers were monitored. Among 283 children, this study found that the anti-HAV seroconversion rates (defined as anti-HAV ≥ 1 mIU/ml) after the first and the third dose were 79.

[Simultaneous determination of 7 female sex hormones in essential oil by high performance liquid chromatography-tandem mass spectrometry with isotope dilution].

A reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 7 female sex hormones (estriol, estradiol, estrone, ethinyloestradiol, dienestrol, hexestrol, diethylstilbestrol) in essential oil was developed. The sample was extracted by ethylacetate-normal hexane solution (2:98, v/v) and the extract was purified by a silica solid phase extraction-based clean-up column. Then, the analytes were separated on an ACQUITY UPLC BEH SHELD RP18 column (100 mm x 2.

Simultaneous determination of 5'-monophosphate nucleotides in infant formulas by HPLC-MS.

A method was developed for simultaneous determination of 5'-monophosphate nucleotides, adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate, inosine 5'-monophosphate, and uridine 5'-monophosphate in infant formulas by high-performance liquid chromatography-mass spectrometry equipped with electrospray ionization source. The complete chromatographic separation of five nucleotides was achieved through a Symmetry C(18) column, after a binary gradient elution with water containing 0.1% formic acid and acetonitrile as mobile phase.

Simultaneous determination of 10 mycotoxins in grain by ultra-high-performance liquid chromatography-tandem mass spectrometry using ¹³C₁₅-deoxynivalenol as internal standard.

An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of 10 mycotoxins in grain was developed. The selected mycotoxins were: deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, nivalenol, fusarenon X, moniliformin, zearalenone, zearalanone, ochratoxin A and ochratoxin B. The samples were extracted with aqueous acetonitrile (84 : 16, v/v) and purified by reliable laboratory-made mixed cartridges.

Simultaneous determination of bovine alpha-lactalbumin and beta-lactoglobulin in infant formulae by ultra-high-performance liquid chromatography-mass spectrometry.

A reliable ultra-high-performance liquid chromatography-mass spectrometry method for simultaneous determination of bovine alpha-lactalbumin (alpha-La) and beta-lactoglobulin (beta-Lg) was developed. Compared to the previous methods, the developed approach with mass spectrometer operated in selected area monitoring mode offered increased speed and enhanced lower detection limit. A linear gradient mobile phase, consisting of (A) water containing 0.

Simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in peanuts and their derivative products by ultra-high-performance liquid chromatography-tandem mass spectrometry.

A reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in peanuts and their derivative products was developed. The sample was extracted by 84% of acetonitrile aqueous solution and the extract was purified by a reliable solid phase extraction-based clean-up method. Then, the analytes were separated on Acquity UPLC HSS T3 column (100 mm x 2.

[The distributions of mosquito vectors carrying Japanese encephalitis virus in Zhejiang province].

Objective: To investigate the natural infection and genotype of Japanese encephalitis virus in mosquitoes and swine in some areas of Zhejiang province.

Methods: Samples of mosquitoes and sera of swine were collected in three counties (Xianju, Longquan and Cixi) of Zhejiang province from May to October in 2007. 10 662 mosquitoes were collected during 2007, of which, C.

[Study on the endocrine disrupting effect of dimethachlon].

Objective: In order to study the endocrine disrupting effect of dimethachlon (NDPS) (estrogenic and antiandrogenic activity).

Methods: To investigate the estrogenic activity of NDPS by different experiments: (1) Carassius auratus received intraperitoneal injections with NDPS (0, 50, 100 and 200 mg/kg) at regular intervals (5 days) for the synthesis of VTG. The estrogenic effects of the treatment were investigated on VTG in plasma and liver measured by ELISA at 14th.

Polychlorinated dibenzo-p-dioxins/furans and polychlorinated biphenyls in human adipose tissue from Zhejiang Province, China.

This is the first report on polychlorinated dibenzo-p-dioxin/furan (PCDD/F) and polychlorinated biphenyl (PCB) contamination of human adipose tissue from China. A total of 24 human adipose tissue samples from a general population in Zhejiang Province were analyzed for PCDD/F and PCB by high-resolution gas chromatography-high-resolution mass spectrometry. Total PCDD/F concentrations in human adipose tissue ranged from 33.

[Study on the model of effect evaluation in AIDS health education among junior middle school students in Hangzhou City].

Objective: To explore the scientific and standardized effect evaluation models of health education, to improve the effect evaluation quality.

Methods: By the stratified cluster sampling method, 270 junior middle school students from six classes of two ordinary junior middle schools were included. The objectives were randomized into three groups, intervention group A (with health education intervention, two questionnaire survey before and after intervention), intervention group B (with health education intervention, one questionnaire survey after intervention), and control group C (only with general hygienic knowledge education, two questionnaire surveys before and after intervention) .

Simultaneous determination of 24 sulfonamide residues in meat by ultra-performance liquid chromatography tandem mass spectrometry.

The present study used the liquid extraction pretreatment method and developed an ultra-performance liquid chromatography triple quadrupole tandem mass spectrometry (UPLC-MS/MS) for the simultaneous determination of 24 kinds of sulfonamide residues in meat. The meat samples were homogenized, extracted and deproteinized by acetonitrile, defatted by n-hexane, and further liquid-liquid extracted by ethyl acetate. All of 24 sulfonamide residues were simultaneously separated and determined by UPLC-MS/MS within 15 min.

[Molecular characterization of hantavirus Zhejiang isolate ZT10 strain from M. fartis].

Objective: To learn about the complete genomic sequence of the Seoul virus strain ZT10 isolated from M. fartis.

Methods: The total RNA was extracted from the infected Vero E6 cells and amplified by RT-PCR.

Simultaneous determination of multi-component mycotoxin contaminants in foods and feeds by ultra-performance liquid chromatography tandem mass spectrometry.

The present study developed an improved analytical method for the simultaneous quantification of 17 kinds of Aspergillus, Fusarium and Penicillium mycotoxin contaminants in foods and feeds by ultra-performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode, and especially focused on the optimization of extraction, clean-up, UPLC separation and MS/MS parameters of analytes. Homogenized samples were sequentially extracted by 84% (v/v) of acetonitrile aqueous solution with the selected internal standard (zearalanone) spiking, SPE clean-up with Mycosep 226 Aflazon+ Multifunctional cartridges, filtration, concentration and secondary filtration. Using double sample injection method, the analytes were separated by UPLC BEH C18 column (100 mm x 2.

Genetic distance of SARS coronavirus from the recent natural case.

Phylogenetic analysis of SARS coronavirus isolates based on the spike gene and protein sequence using Neighbor-Joining, maximum likelihood and Bayesian inference methods indicated that a recent human SARS-CoV isolate was closer to some human SARS-CoV isolates from earlier epidemic phase than to the SARS-CoV-like viruses isolated from wild animals during previous epidemic phase. A reasonable judgment based on phylogenetic relationship and sequence variations it is likely that the recent human SARS-CoV isolate is closer to an unknown SARS-CoV predecessor.

The simultaneous separation and determination of chloropropanols in soy sauce and other flavoring with gas chromatography-mass spectrometry in negative chemical and electron impact ionization modes.

Both gas chromatography-mass spectrometry in electron ionization (GC-MS-EI) and negative chemical ionization (GC-MS-NCI) modes are reported in this paper for the simultaneous determination of 1,3-dichloropropan-2-ol (1,3-DCP), 2,3-dichloropropan-1-ol (2,3-DCP), 3-chloropropane-1,2-diol (3-MCPD) and 2-chloropropane-1,3-diol (2-MCPD) in soy sauce and other flavoring. D(5)-3-MCPD (for 3-MCPD and 2-MCPD) and d(5)-1,3-DCP (for 1,3-DCP and 2,3-DCP) were used as the deuterium isotopic labelled internal standards. The feasibility of using heptafluorobutyric anhydride modified with triethylamine (HFBA-Et(3)N) as a new derivatization reagent to replace heptafluorobutyrylimidazole (HFBI) is proposed.

Molecular evolution and multilocus sequence typing of 145 strains of SARS-CoV.

In this study, we have identified 876 polymorphism sites in 145 complete or partial genomes of SARS-CoV available in the NCBI GenBank. One hundred and seventy-four of these sites existed in two or more SARS-CoV genome sequences. According to the sequence polymorphism, all SARS-CoVs can be divided into three groups: (I) group 1, animal-origin viruses (such as SARS-CoV SZ1, SZ3, SZ13 and SZ16); (II) group 2, all viruses with clinical origin during first epidemic; and (III) group 3, SARS-CoV GD03T0013.

[Evaluation of intervention strategy and measures on the control of intestinal parasitic infections].

Objective: To evaluate the effect of intervention strategy and measures for intestinal parasite control in Zhejiang Province.

Methods: The protective rate (PR) and the index of effectiveness (IE) on the overall prevalence of parasites after and before interventions for intestinal parasitic infections were compared in 30 villages of 10 counties randomly selected as investigation spots.

Results: After the implementation of the interventions in the past decade, the total parasite prevalence declined significantly from 77.

Molecular biological analysis of genotyping and phylogeny of severe acute respiratory syndrome associated coronavirus.

Background: SARS-CoV is the causative agent of severe acute respiratory syndrome (SARS) which has been associated with outbreaks of SARS in Guangdong, Hong Kong and Beijing of China, and other regions worldwide. SARS-CoV from human has shown some variations but its origin is still unknown. The genotyping and phylogeny of SARS-CoV were analyzed and reported in this paper.

Severe acute respiratory syndrome-associated coronavirus genotype and its characterization.

Objective: To study the severe acute respiratory syndrome (SARS)-associated coronavirus genotype and its characteristics.

Methods: A SARS-associated coronavirus isolate named ZJ01 was obtained from throat swab samples taken from a patient in Hangzhou, Zhejing province. The complete genome sequence of ZJ01 consisted of 29,715 bp (GenBank accession: AY297028, version: gi: 30910859).