3D Method for the Volumetric Evaluation and Visualisation of Dental Biofilms: A Proof-of-Principle Study.

Background And Objective: Traditional and planimetric plaque indices rely on plaque-disclosing agents and cannot quantify three-dimensional (3D) structures of dental biofilms. We introduce a novel computer-assisted method for evaluating and visualising plaque volume using intraoral scans (IOSs).

Materials And Methods: This was a 4-day, non-brushing, plaque-regrowth study (n = 15).

Prediction of intracranial aneurysm rupture from computed tomography angiography using an automated artificial intelligence framework.

Intracranial aneurysms (IAs) are common vascular pathologies with a risk of fatal rupture. Human assessment of rupture risk is error prone, and treatment decision for unruptured IAs often rely on expert opinion and institutional policy. Therefore, we aimed to develop a computer-assisted aneurysm rupture prediction framework to help guide the decision-making process and create future decision criteria.

A systematic review of health promoting effects of consumption of whey-based fermented products on adults.

Introduction: Fermented whey-based products show significant potential as functional foods, owing to their rich nutritional profile and the generation of bioactive compounds during fermentation. This systematic narrative review evaluates the health effects of fermented-whey consumption based on evidence from human studies in adults.

Methods: A systematic literature search was conducted using electronic databases including, PubMed, Scopus, and Cochrane Library for studies published between 1.

Psychometric Validation of the Ultrashort Slovenian (OHIP-SVN5) and Croatian (OHIP-CRO5) Oral Health Impact Profile Questionnaires.

Aim: The study tested the validity, reliability and responsiveness of the ultrashort Slovenian and Croatian versions of the 5-item Oral Health Impact Profile (OHIP-5).

Methods: A total of 663 participants from the general population (400 from Slovenia and 223 from Croatia), 68 students (38 from Slovenia and 30 from Croatia), and 60 dental patients with treatment needs (30 from Slovenia and 30 from Croatia) were included in this investigation. Internal consistency was assessed using Cronbach's alpha, and test-retest reliability was evaluated with intraclass correlation coefficients (ICC).

Mind the Gap: A Retrospective Study of Discrepancies in Self-Reported and Administrative Database-Identified Mental Health Issues in Slovenia.

Background: This study assessed discrepancies between self-reported and administrative data sources in identifying mental health issues in Slovenia, and investigated associated socio-demographic factors.

Methods: Data were linked from the 2019 Slovenian European Health Interview Survey (EHIS; n=9,900) and national health administrative databases capturing inpatient hospitalisations, outpatient prescription drugs and mental health-related sick leave. Mental health issues were identified in EHIS by self-report and in administrative databases using diagnostic codes and medication claims.

The Effect of a Hatha Yoga Exercise Programme with Segmental Stabilisation Exercises on Trunk Flexibility.

Introduction: In addition to sufficient trunk muscle endurance, adequate trunk flexibility, i.e. trunk muscle extensibility and spinal mobility, is an important element for the stability of the spine and pelvis.

Reliability and Responsiveness of Endoscopic Indices for Assessing Crohn's Disease Postoperative Recurrence in the PREVENT trial.

Background And Aims: Assessing endoscopic activity is integral in the management of postoperative Crohn's disease (CD). We aimed to comprehensively characterize the reliability and responsiveness of different endoscopic instruments when used to assess postoperative CD activity.

Methods: Ileocolonoscopy videos (n=70) from the PREVENT trial were reviewed by three blinded central readers.

Screen first, vaccinate later: Enhancing tuberculosis vaccination safety through newborn immunodeficiency screening.

Tuberculosis (TB) remains a global health challenge, with around 10 million new cases reported annually and multidrug-resistant strains complicating control efforts. Although incidence has declined in many high-income regions, neonatal populations remain vulnerable, underscoring the continued role of Bacillus Calmette-Guérin (BCG) vaccination. BCG vaccination provides strong protection against severe forms of TB in infancy, though its efficacy against pulmonary disease in adolescents and adults is modest.

Magnetomechanical Detachment of Bacterial Biofilms Using Anisotropic Magnetic Iron Oxide Nanochains.

Bacterial biofilms attach to various surfaces and represent an important clinical and public health problem, as they are highly recalcitrant and are often associated with chronic, nonhealing diseases and healthcare-associated infections. Antibacterial agents are often not sufficient for their elimination and have to be combined with mechanical removal. Mechanical forces can be generated by actuating nonspherical (anisotropic) magnetically responsive nanoparticles in a rotating magnetic field.

Patient Perspectives on the Quality of Primary Care for Chronic Conditions in Slovenia: Evidence from the PaRIS Survey.

Background: Chronic conditions (CCs) represent a growing global burden, with profound implications for quality of life and the long-term sustainability of health systems. High-quality primary care is fundamental to the effective management of CCs, yet patient-reported experiences of care quality remain inconsistent. This study aimed to assess the quality of primary care delivered to patients with CCs in Slovenia and to identify factors associated with perceived care quality.

Using Explainable AI to Characterize Features in the Mirai Mammographic Breast Cancer Risk Prediction Model.

Purpose To evaluate whether features extracted by Mirai can be aligned with mammographic observations, and contribute meaningfully to the prediction. Materials and Methods This retrospective study examined the correlation of 512 Mirai features with mammographic observations in terms of receptive field and anatomic location. A total of 29,374 screening examinations with mammograms (10,415 women, mean age at examination 60 [SD: 11] years) from the EMBED Dataset (2013-2020) were used to evaluate feature importance using a feature-centric explainable AI pipeline.

Cardiovascular health and cognition in older adults: Variations across recruitment models and European countries in the MOPEAD project.

BackgroundThe Models of Patient Engagement for Alzheimer's Disease (MOPEAD) project aimed to identify the most effective and cost-efficient recruitment model for detecting prodromal and mild Alzheimer's disease (AD) across five European countries.ObjectiveTo examine differences in cardiovascular risk factors and cognitive performance among countries and recruitment models using MOPEAD data.MethodsIndividuals aged 65-85 with a high risk for prodromal or mild AD were included.

Electrophysiological recordings reveal photoreceptor coupling in the dorsal rim areas of honeybee and bumblebee eyes.

Many insects rely on skylight polarization patterns to navigate their habitats. To perform this vital task, most insect species have evolved specialized ommatidia in the dorsal rim area (DRA) of their compound eyes, which are adapted to detect linearly polarized light in large patches of the sky. In this study, we conducted electrophysiological recordings of ultraviolet-sensitive photoreceptors in the DRA and other regions of the compound eyes in honeybees () and bumblebees () to map their receptive fields (RFs).

RNA -splicing to rescue β-catenin: A novel approach for treating CTNNB1-Haploinsufficiency disorder.

Loss-of-function mutations in the gene cause β-catenin deficiency, resulting in CTNNB1 syndrome, a rare neurodevelopmental disorder characterized by motor and cognitive impairments. Given the wide variety of mutations across and its dosage sensitivity, a mutation-independent therapeutic approach that preserves endogenous gene regulation is critically needed. This study introduces spliceosome-mediated RNA -splicing as a novel approach to restore β-catenin production.

The influence of glycine on -lactoglobulin amyloid fibril formation - computer simulation study.

Amyloids are protein aggregates involved in various protein condensation diseases. Our study aims to investigate the influence of glycine on the fibrillization mechanism of -lactoglobulin (BLG), a model protein known to form amyloid fibrils from hydrolysed peptides in low pH aqueous solutions. We conducted atomistic molecular dynamics simulations of aqueous solutions of native and unfolded BLG in glycine buffer at pH 2.

Synthesis of a -Tricarbonylrhenium(I) Complex with Pyrithione, Its Physicochemical Characterization, and Assessment of Biological Effects.

Research on rhenium complexes containing -tricarbonyl fragments has been on the rise in recent decades. Some complexes of this type exhibit advantageous properties that can be utilized in diagnostic and therapeutic applications. Herein, we report on the synthesis, structural characterization, solution speciation, and biological activity with mode of action studies of a new -tricarbonylrhenium-(I) complex with pyrithione ligand -[Re-(CO)(pyrithionato)-(benzonitrile)] ().

Age, gender, and BMI in presentation of primary hyperparathyroidism: a single-center experience.

Background: Primary hyperparathyroidism (PHPT) is a relatively common disease with considerable heterogeneity. We aimed to assess the impact of age, gender, and body mass index (BMI) on the presentation of PHPT.

Material And Methods: We retrospectively analyzed the baseline biochemical status, symptoms, renal manifestations, and bone mineral density (BMD) of patients diagnosed with PHPT at the national tertiary endocrine referral clinic from January 2004 to December 2016.

Hypertensive acute heart failure: a critical perspective on definition, epidemiology, pathophysiology, and prognosis-a narrative review: a joint session with the Romanian Society of Cardiology (part II).

Hypertensive acute heart failure (HT-AHF) has historically been recognized as a distinct clinical phenotype of AHF, characterized by acute pulmonary congestion in the context of elevated systolic blood pressure (SBP), typically > 140 mmHg. However, emerging evidence has begun to challenge the diagnostic accuracy, clinical utility, and relevance of this category. A main criticism of HT-AHF is its considerable overlap with other AHF clinical profiles, including acute decompensated heart failure (ADHF) and acute pulmonary oedema (APO).

Occupants and surface types drive microbial dynamics in controlled indoor environments.

Background: Indoor microbial communities play a critical role in influencing indoor environmental quality and human health and are shaped by occupant activity, surface characteristics, and environmental conditions. While previous studies have examined these factors individually, systematic evaluations of their combined interactions, particularly involving Heating, Ventilation, and Air Conditioning (HVAC) and drainage systems, remain limited. This controlled, long-term (1.

Towards consensus: The need for standardised definitions in Long (post) COVID care in 34 European countries.

Background: The COVID-19 pandemic has significantly impacted global healthcare systems, leading to challenges in managing Long COVID. Variations in definitions and diagnostic criteria across Europe hinder recognition and treatment efforts. This study aims to analyse and compare the definitions of Long COVID used in 34 European countries.

Water-Based Pharmacophore Modeling in Kinase Inhibitor Design: A Case Study on Fyn and Lyn Protein Kinases.

Water-based pharmacophore modeling is an emerging approach in inhibitor design that leverages the dynamics of explicit water molecules within ligand-free, water-filled binding sites to derive 3D pharmacophores for virtual screening. In this study, we assess the potential of this strategy through a case study targeting the ATP binding sites of Fyn and Lyn protein kinases─members of the Src family that have been less explored in anticancer drug discovery compared to other family members. Molecular dynamics simulations of multiple kinase structures were used to generate and validate several water-derived pharmacophores, which were subsequently employed to screen chemically diverse libraries of compounds.

Asymmetric T-segment binding and gate dynamics govern the final stages of the type IIA topoisomerase catalytic cycle.

Type IIA DNA topoisomerases are molecular nanomachines that alter DNA topology during essential cellular processes. The final steps of their catalytic cycle, after translocation of the transported (T-) segment into the C-gate, are still not fully understood. Here, we performed all-atom molecular dynamics simulations of several conformational states of Saccharomyces cerevisiae topoisomerase IIA, each with a T-segment inserted into the C-gate.