Effect of aqueous green coffee extract () on the intestinal health of Wistar rats fed with a hypercaloric and hyperlipidic diet.

Inadequate eating habits, such as excessive caloric intake and diets high in saturated fats, are associated with the development of obesity and intestinal alterations. Green coffee is rich in bioactive antioxidant compounds, which may assist in obesity management. This study aimed to evaluate the effect of aqueous green coffee extract on intestinal health in Wistar rats fed a high-fat diet.

Unraveling hydride dynamics on cubic palladium nanoparticles.

Palladium-based materials exhibit a high affinity for hydrogen molecules, enabling the effortless formation of a hydride phase. This property is widely exploited in several catalytic reactions and hydrogen storage materials. However, the effects of morphological parameters, support interactions, and formation kinetics remain incompletely understood.

Self-emulsifying and lyotropic nanocarriers enhance solubility and antiparasitic efficacy of artesunic acid against different strains of Schistosoma mansoni.

Schistosomiasis currently relies on the long-term use of a single drug, praziquantel (PZQ). Artesunic acid (AcART) has emerged as a promising alternative, as it is efficient against juvenile worms. However, like PZQ, it has low aqueous solubility.

Thoracolumbar Fractures: Historical Systems and Advancements With the AO Spine Classification.

Study DesignSystematic Review.ObjectiveTo describe the historical classifications of thoracolumbar injuries and their evolution into the AO Spine Thoracolumbar Injury Classification System.MethodsA systematic review of MEDLINE, EMBASE and Cochrane Databases was performed in keeping with Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines.

Effect of charcoal-based dentifrices on the surface integrity and gloss of lithium disilicate glass-ceramics.

Objectives: To evaluate the effects of commercially charcoal-based dentifrices on the surface roughness and gloss retention of glazed lithium disilicate glass-ceramics subjected to simulated toothbrushing.

Materials And Methods: Seventy-two glazed lithium disilicate specimens (IPS e.max CAD) were randomly assigned to three dentifrice groups (n = 24): two charcoal-based (Carvvo (CVV), Curaprox Black is White (CBW)) and one conventional (Colgate Total 12 (C12)), the control.

Laboratory Identification of Lupus Anticoagulant (LA) Using Different Activated Partial Thromboplastin Time (APTT) Assays.

Introduction: The International Society of Thrombosis and Hemostasis (ISTH) guidelines suggest a three-step evaluation for the detection of lupus anticoagulant (LA), including screening, mixing, and confirmation. According to the guidelines, the LA assay based on activated partial thromboplastin time (APTT) should include an initial screening step followed by a confirmatory step that uses a higher concentration of phospholipids in either bilayer or hexagonal form. For the activator, the guidelines recommend using silica, though ellagic acid is also an option.

IoT and Blockchain for Support for Smart Contracts Through TpM.

This paper investigates the integration of Internet of things (IoT) technology with blockchain to enhance transparency, accountability, and operational efficiency in smart contract execution for IoT ecosystems. The proposed approach extends the Three-Phase Methodology (TpM) by introducing an innovative entity, the IoT Operator, which acts as a custody caretaker, contract enforcer, and mediator. By leveraging blockchain's secure and immutable ledger, the IoT Operator ensures the reliable monitoring and governance of IoT applications.

Metabolic Heterogeneity Confers Differences in the Tumor Microenvironment of Aggressive Types of Melanomas.

Background: Melanoma affects skin and mucosa and can be particularly aggressive when the lesion is an advanced cutaneous tumor or located in the sinonasal or oral mucosa. Reprogramming of energy metabolism has been defined as a hallmark of cancer; so this study aimed to verify the expression of proteins related to metabolism and cellular proliferation.

Methods: Immunohistochemical analysis with antibodies adipophilin, FASN, GLUT-1, HIF-1α, and Ki-67 was performed in a series of 28 sinonasal melanomas (SM), 16 oral melanomas (OM), and 39 cutaneous melanomas (CM).

Adjunctive methylene blue-based antimicrobial photodynamic therapy in the re-instrumentation of residual periodontal pockets in individuals with down syndrome: A randomized controlled clinical trial.

Objective: The aim of this study was to evaluate the use of a single session antimicrobial photodynamic therapy (aPDT) protocol in the re-instrumentation of residual periodontal pockets in individuals with Down Syndrome (DS).

Methods: 30 individuals with DS and stages I-III grade B periodontitis were allocated into the following groups: SI (n = 17): subgingival instrumentation (SI) in a single session; SI+aPDT (n = 17): SI in single session in residual pockets with probing depth (PD) ≥4 mm and bleeding on probing (BoP), methylene blue irrigation (100 µg/ml) after 60 s, and diode laser irradiation at 100 mW for 50 s. Periodontal clinical parameters PD, BoP, clinical attachment level (CAL) and immunological analysis interleukin (IL)-17, IL-1B, IL-6, IL-8 were evaluated at baseline and on 60 and 90 days.

Effect of Butyl Toluidine Blue-Mediated Photodynamic Therapy on Periodontal Healing in Type 2 Diabetic Patients. A Randomized Clinical Trial.

Objective: We sought to evaluate the photodynamic effect of butyl toluidine blue (BuTB) as an adjuvant therapy for the treatment of residual periodontal pockets in patients with type 2 diabetes mellitus (DM2).

Methods: This randomized clinical study comprised 42 DM2 patients with residual periodontal pockets, defined as probing pocket depth (PPD) ≥ 4 mm and bleeding on probing (BOP). Subjects were assigned to one of the following groups: Subgingival instrumentation (SI): SI performed in a single session; and SI + antimicrobial photodynamic therapy (aPDT): SI in a single session, followed by pocket irrigation with BuTB and irradiation with a 660 nm diode laser at 100 mW for 50s.

Green Chemistry and Multivariate Optimization in the Extraction of Phenolic Compounds: The Potential of NaDES in Alternative Raw Materials for Expanded Extrudates.

Phenolic compounds are secondary metabolites widely distributed among plants, with bioactive properties, especially antioxidant activity. The search for sustainable extraction methods has driven the use of natural deep eutectic solvents (NaDESs), formed by combinations of natural compounds, such as organic acids, sugars, alcohols, and amino acids. This study optimized NaDES (sorbitol, citric acid, and glycine) efficiency and compared it to that of 70% methanol solution in extracting total soluble phenolic compounds (TSPCs) from six flours matrices-corn, buckwheat, biofortified orange sweet potato, red lentil, Sudan grass, and chickpea-before and after thermoplastic extrusion cooking.

Oral Manifestations, Dental Interventions, and Clinical Outcomes in Hospitalized COVID-19 Patients: A Two-Year Cohort Study in São Paulo, Brazil.

To investigate the demographic, epidemiological, and medical profiles of hospitalized COVID-19 patients who received dental care, and to identify their main oral health needs. This retrospective, descriptive cohort study analyzed medical and dental records of patients hospitalized with COVID-19 at a private tertiary hospital in São Paulo, Brazil, from January 2020 to March 2022. The data collected included demographic variables, comorbidities, length of hospitalization, need for respiratory support, clinical outcomes, dental diagnoses, and procedures performed.

Diagnostic Accuracy of Ultrasonography for Detecting Posterior Ligamentous Complex Injuries of Thoracolumbar Trauma: An Updated Systematic Review With Meta-Analysis.

Study DesignSystematic review with meta-analysis.ObjectiveThis study aimed to assess the diagnostic accuracy of USG for detecting PLC injuries.MethodsA systematic search was conducted in PubMed, EMBASE, and Web of Science.

Impact of Active Surveillance on Nephrometric Scores of Bosniak III and IV Renal Cysts.

Purpose: Active surveillance (AS) has been widely explored for small renal masses, but its role in complex renal cysts (CRCs) categorized as Bosniak (Bk) III and IV remains unclear. This study aims to evaluate changes in nephrometry scores (s-RENAL and s-PADUA) during AS and their potential impact on surgical complexity.

Methods: A retrospective, multicentric, observational study was conducted, analyzing 101 CRCs from 95 patients across three institutions.

The identification of adenylyl cyclase modulators as potential receptors for 6-nitrodopamine in human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and their relevance in heart inotropism.

6-Nitrodopamine (6-ND) has potent positive chronotropic and inotropic effects. At a very low dose, i.e.

Effects of different antihypertensive drug classes on central and ambulatory blood pressure in resistant hypertension: A randomized clinical trial.

Aims: This study compares sequential nephron blockade (SNB) and renin-angiotensin system sequential blockade (RASSB) in patients with resistant hypertension (RHTN), evaluating central systolic pressure, arterial stiffness, pulse pressure (PP) and ambulatory blood pressure monitoring (ABPM).

Methods: Seventy-two RHTN patients were randomly assigned to SNB (n = 35, 22 females, 13 males) or RASSB (n = 37, 22 females, 15 males). SNB included multiple diuretics (furosemide, spironolactone, hydrochlorothiazide) to enhance natriuresis and counter intra-renal compensatory mechanisms.

Malt lymphoma affecting the oral cavity: a clinical, pathologic and genetic study of malt 1 gene translocation.

Objective: To investigate the clinicopathological, immunohistochemical and molecular features of MALT Lymphoma affecting the oral cavity.

Methods: Oral pathology files of a Brazilian institution were searched for cases of oral MALT lymphoma. New H&E-stained slides and immunohistochemistry reactions were evaluated for diagnosis confirmation.

Morphometric and volumetric analysis of the frontal sinus in a brazilian population using cone beam computed tomography: a forensic approach for sex, age and facial morphology determination.

The aim of this study was to evaluate the linear and volumetric measurements of the frontal sinus (FS), using cone beam computed tomography (CBCT) scans, for a discriminatory analysis of gender, age and facial skeletal pattern in a Brazilian population. A total of 300 CBCT scans were analyzed, measuring the height, width, length and volume of the FS. The measurements were divided into groups: sex, age (<20 to >40 years and >40 years) and facial skeletal pattern classes I, II and III.

Ultrack: pushing the limits of cell tracking across biological scales.

Tracking live cells across two-dimensional, three-dimensional (3D) and multichannel time-lapse recordings is crucial for understanding tissue-scale biological processes. Despite advancements in imaging technology, accurately tracking cells remains challenging, particularly in complex and crowded tissues where cell segmentation is often ambiguous. We present Ultrack, a versatile and scalable cell tracking method that tackles this challenge by considering candidate segmentations derived from multiple algorithms and parameter sets.

Forest Age Rivals Climate to Explain Reproductive Allocation Patterns in Forest Ecosystems Globally.

Forest allocation of net primary productivity (NPP) to reproduction (carbon required for flowers, fruits, and seeds) is poorly quantified globally, despite its critical role in forest regeneration and a well-supported trade-off with allocation to growth. Here, we present the first global synthesis of a biometric proxy for forest reproductive allocation (RA) across environmental and stand age gradients from a compiled dataset of 824 observations across 393 sites. We find that ecosystem-scale RA increases ~60% from boreal to tropical forests.

Understanding the evolving treatment landscape of hidradenitis suppurativa: An analysis of All of Us.

Background: Hidradenitis suppurativa (HS) is a chronic skin condition with significant burden for affected patients. The development of new therapies in the last decade has brought hope for better patient outcomes, but understanding the use of these new drug classes is necessary to ensure patient access to care.

Methods: We analysed data from 2636 HS patients within NIH's All of Us research program from June 2017 and October 1, 2023 to examine the likelihood of patients receiving certain classes of drugs based on socio-demographic factors and comorbid health conditions, as well as how these drug classes impact quality of life.

Is Palivizumabe a Protective Factor for the Development of Asthma in Children? A Systematic Review With Metanalysis.

Introduction: Premature infants are at increased risk of developing chronic respiratory diseases, predisposing them to severe infections, such as those caused by respiratory syncytial virus (RSV). Palivizumab reduces the severity of RSV infections in high-risk children; however, its relationship with asthma development in premature infants remains unclear.

Objective: This systematic review with meta-analysis aimed to review the literature and assess whether prophylaxis with palivizumab protects premature infants without congenital heart disease from developing asthma.

Rosemary Extract: Phytochemical Composition and Potential for Eliminating Polymicrobial Biofilm of and Multidrug-Resistant Bacteria.

Herbal medicines can be promising for the treatment of infections caused by multidrug-resistant microorganisms. This study aimed to evaluate (Rosemary) hydroalcoholic extract (RHE) regarding its phytochemical composition and potential for eliminating polymicrobial biofilm of with multidrug-resistant bacteria (, , and ). The extraction and quantification of the extract (flavonoids and phenols) were performed, and its antioxidant activity (DPPH) and the presence of bio-active compounds were investigated using high-performance liquid chromatography with Diode Array Detection (HPLC-DAD) and Gas Chromatography-Mass Spectrometry (GC-MS).

6-Nitrodopamine Release From Mouse Seminal Vesicles Is Dependent on Endothelial Nitric Oxide Synthase (eNOS) Activation.

Human seminal vesicles present basal release of epithelium-derived 6-nitrodopamine (6-ND) and this catecholamine potentiates noradrenaline-induced contractions. Since nitric oxide synthase (NOS) activation is a determining factor involved in 6-ND biosynthesis, this study aimed to investigate which NOS isoform is responsible for the 6-ND release in mouse isolated seminal vesicles (MISV). Male control, eNOS, NOS, iNOS, and e/n/iNOS mice were used.