Occurrence of Listeria spp. and Listeria monocytogenes in half-carcasses, meat cuts, equipment, and the environment of bovine slaughterhouses in Brazil.

Human listeriosis is a severe food-borne illness, with fatality rates ranging from 20 to 30 %. In Brazil, despite being an underdiagnosed and underreported disease, the presence of the microorganism in food has been the subject of important studies. However, its occurrence in slaughterhouse environments has received little attention in recent years.

Myo-inositol is a key regulator of avian metabolism: From mechanisms to seasonal behavior.

Being naturally hyperglycemic and insulin insensitive, birds maintain plasma glucose levels twice as high as mammals of similar size. Recent evidence suggests that perturbation of myo-inositol (MI) plays a role in mammalian hyperglycemic regulation. Using an integrative approach, we identify a fundamental role of MI in avian metabolism.

The source-sink dynamics of Plasmodium vivax may undermine malaria elimination efforts in the Amazon: an epidemiological and population genomic study.

Background: Brazil's progress toward malaria elimination has stalled and 163,000 new cases (more than 80% caused by Plasmodium vivax) were recorded in the Brazilian Amazon in 2023. We hypothesize that human mobility continues to disperse parasites from hotspots to areas with decreasing endemicity.

Methods: We analyzed 5.

A Randomized Controlled Trial Comparing Two Techniques of Enteral Feeding Tube Placement in Critically Ill Newborn Infants.

Background: Feeding intolerance can hinder enteral nutrition in infants, often necessitating transpyloric tube feeding. However, the success of transpyloric tube placement varies.

Purpose: To compare the rate of correct transpyloric tube placement in infants with and without gastric air insufflation, and to assess complications associated with postpyloric feeding.

Efficacy and Safety of Bariatric Surgery in Well-Compensated Liver Cirrhosis: A Systematic Review and a Single-Arm Meta-analysis.

Introduction: The global rise in obesity and liver disease underscores the significance of bariatric and metabolic surgery (BMS) in treating severe obesity and improving liver function. Although liver failure is often considered a contraindication for elective surgeries, the role of bariatric surgery in patients with compensated cirrhosis has been increasingly explored.

Methods: We systematically searched PubMed, Embase, and Cochrane without temporal restrictions, in January 2025 for randomized controlled trials and observational studies.

Functionalized Silk Fibroin and Mucin Hybrid Material for Targeted EGF and Papain Delivery in Wound Healing.

Silk fibroin (SF) and mucin are extensively recognized as promising biomaterials for wound dressings due to their outstanding biocompatibility, biodegradability, and ability to support cell growth and tissue regeneration. In this study, we developed a hybrid SF/mucin wound dressing (HYB) using tetrazine and norbornene click chemistry to enhance its structural and functional properties. The robust assembly resulted in a dual-phase material with a dense SF membrane and a porous mucin hydrogel (MH).

Early Withdrawal of Aspirin after PCI in Acute Coronary Syndromes.

Background: Whether potent P2Y12 inhibitor monotherapy without aspirin initiated shortly after successful percutaneous coronary intervention (PCI) is effective and safe for patients with acute coronary syndromes is unclear.

Methods: We conducted a multicenter, open-label, randomized trial in Brazil involving patients with acute coronary syndromes who had undergone successful PCI. Patients were assigned in a 1:1 ratio within the first 4 days of hospitalization to stop treatment with aspirin and receive potent P2Y12 inhibitor monotherapy (ticagrelor or prasugrel) or to receive dual antiplatelet therapy (aspirin and a potent P2Y12 inhibitor) for 12 months.

Molecular diagnosis of Chagas disease in patients with megaesophagus exhibiting negative or inconclusive serological results.

Chagasic megaesophagus is a relatively uncommon clinical manifestation in individuals with chronic Chagas disease (CD), and it has not been extensively documented in literature. However, individuals may exhibit negative or inconclusive serology for CD. This study aimed to assess the performance of molecular diagnostics for CD in participants with these conditions.

Interindividual genetic differences drive discordance between serum calcidiol and calcitriol concentrations in females.

Vitamin D insufficiency (VDI) is primarily determined by serum levels of calcidiol (25(OH)D3), which serves as a biomarker for the less abundant but most potent bioactive metabolite, calcitriol (1,25(OH)2D3). However, population studies often show discordance between calcidiol and calcitriol. Here, a genetically diverse population of seven inbred mouse strains was used to investigate the role of interindividual genetic differences in driving calcidiol-to-calcitriol discordance under vitamin D sufficient (VDS) vs.

Efficacy and safety comparison of single anastomosis sleeve ileal bypass versus one anastomosis gastric bypass: a systematic review and meta-analysis.

Background: Obesity and its associated medical problems are a global health concern. Combining metabolic and restrictive approaches may yield better results, as seen in one anastomosis gastric bypass (OAGB) and single anastomosis sleeve ileal (SASI) bypass, which evolved from Santoro's technique.

Objectives: To compare the efficacy and safety of SASI bypass versus OAGB, focusing on weight loss, comorbidity remission, nutritional outcomes, and complications.

Effects of Lycopene Supplementation on Bone Tissue: A Systematic Review of Clinical and Preclinical Evidence.

: Bone tissue undergoes continuous remodeling, and imbalances in this process can lead to osteometabolic disorders, such as osteoporosis. Thus, bioactive compounds, like lycopene (LYC), have been investigated for their potential protective effects in bone health. This systematic review (SR) aimed to evaluate the effects of LYC supplementation on bone tissue.

Effects of Incorporation of Essential Oils in the Jersey Cow Diet on the Quality of Produced Dairy Products (Milk, Cream, and Colonial Cheese).

Essential oil blends (EOBs) have been increasingly studied for their multifunctional benefits in animal nutrition and food science. This study evaluates the impact of an EOB composed of eucalyptus and peppermint oil on the physicochemical, microbiological, and sensory characteristics of dairy products-milk, cream, and colonial cheese. Forty lactating cows were assigned to two groups: control and EOB addition (3.

Thoracolumbar Fractures: Historical Systems and Advancements With the AO Spine Classification.

Study DesignSystematic Review.ObjectiveTo describe the historical classifications of thoracolumbar injuries and their evolution into the AO Spine Thoracolumbar Injury Classification System.MethodsA systematic review of MEDLINE, EMBASE and Cochrane Databases was performed in keeping with Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines.

Effect of charcoal-based dentifrices on the surface integrity and gloss of lithium disilicate glass-ceramics.

Objectives: To evaluate the effects of commercially charcoal-based dentifrices on the surface roughness and gloss retention of glazed lithium disilicate glass-ceramics subjected to simulated toothbrushing.

Materials And Methods: Seventy-two glazed lithium disilicate specimens (IPS e.max CAD) were randomly assigned to three dentifrice groups (n = 24): two charcoal-based (Carvvo (CVV), Curaprox Black is White (CBW)) and one conventional (Colgate Total 12 (C12)), the control.

Adjunctive methylene blue-based antimicrobial photodynamic therapy in the re-instrumentation of residual periodontal pockets in individuals with down syndrome: A randomized controlled clinical trial.

Objective: The aim of this study was to evaluate the use of a single session antimicrobial photodynamic therapy (aPDT) protocol in the re-instrumentation of residual periodontal pockets in individuals with Down Syndrome (DS).

Methods: 30 individuals with DS and stages I-III grade B periodontitis were allocated into the following groups: SI (n = 17): subgingival instrumentation (SI) in a single session; SI+aPDT (n = 17): SI in single session in residual pockets with probing depth (PD) ≥4 mm and bleeding on probing (BoP), methylene blue irrigation (100 µg/ml) after 60 s, and diode laser irradiation at 100 mW for 50 s. Periodontal clinical parameters PD, BoP, clinical attachment level (CAL) and immunological analysis interleukin (IL)-17, IL-1B, IL-6, IL-8 were evaluated at baseline and on 60 and 90 days.

Effect of Butyl Toluidine Blue-Mediated Photodynamic Therapy on Periodontal Healing in Type 2 Diabetic Patients. A Randomized Clinical Trial.

Objective: We sought to evaluate the photodynamic effect of butyl toluidine blue (BuTB) as an adjuvant therapy for the treatment of residual periodontal pockets in patients with type 2 diabetes mellitus (DM2).

Methods: This randomized clinical study comprised 42 DM2 patients with residual periodontal pockets, defined as probing pocket depth (PPD) ≥ 4 mm and bleeding on probing (BOP). Subjects were assigned to one of the following groups: Subgingival instrumentation (SI): SI performed in a single session; and SI + antimicrobial photodynamic therapy (aPDT): SI in a single session, followed by pocket irrigation with BuTB and irradiation with a 660 nm diode laser at 100 mW for 50s.

Diagnostic Accuracy of Ultrasonography for Detecting Posterior Ligamentous Complex Injuries of Thoracolumbar Trauma: An Updated Systematic Review With Meta-Analysis.

Study DesignSystematic review with meta-analysis.ObjectiveThis study aimed to assess the diagnostic accuracy of USG for detecting PLC injuries.MethodsA systematic search was conducted in PubMed, EMBASE, and Web of Science.

Impact of Active Surveillance on Nephrometric Scores of Bosniak III and IV Renal Cysts.

Purpose: Active surveillance (AS) has been widely explored for small renal masses, but its role in complex renal cysts (CRCs) categorized as Bosniak (Bk) III and IV remains unclear. This study aims to evaluate changes in nephrometry scores (s-RENAL and s-PADUA) during AS and their potential impact on surgical complexity.

Methods: A retrospective, multicentric, observational study was conducted, analyzing 101 CRCs from 95 patients across three institutions.

The identification of adenylyl cyclase modulators as potential receptors for 6-nitrodopamine in human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and their relevance in heart inotropism.

6-Nitrodopamine (6-ND) has potent positive chronotropic and inotropic effects. At a very low dose, i.e.

Effects of different antihypertensive drug classes on central and ambulatory blood pressure in resistant hypertension: A randomized clinical trial.

Aims: This study compares sequential nephron blockade (SNB) and renin-angiotensin system sequential blockade (RASSB) in patients with resistant hypertension (RHTN), evaluating central systolic pressure, arterial stiffness, pulse pressure (PP) and ambulatory blood pressure monitoring (ABPM).

Methods: Seventy-two RHTN patients were randomly assigned to SNB (n = 35, 22 females, 13 males) or RASSB (n = 37, 22 females, 15 males). SNB included multiple diuretics (furosemide, spironolactone, hydrochlorothiazide) to enhance natriuresis and counter intra-renal compensatory mechanisms.

Morphometric and volumetric analysis of the frontal sinus in a brazilian population using cone beam computed tomography: a forensic approach for sex, age and facial morphology determination.

The aim of this study was to evaluate the linear and volumetric measurements of the frontal sinus (FS), using cone beam computed tomography (CBCT) scans, for a discriminatory analysis of gender, age and facial skeletal pattern in a Brazilian population. A total of 300 CBCT scans were analyzed, measuring the height, width, length and volume of the FS. The measurements were divided into groups: sex, age (<20 to >40 years and >40 years) and facial skeletal pattern classes I, II and III.

Ultrack: pushing the limits of cell tracking across biological scales.

Tracking live cells across two-dimensional, three-dimensional (3D) and multichannel time-lapse recordings is crucial for understanding tissue-scale biological processes. Despite advancements in imaging technology, accurately tracking cells remains challenging, particularly in complex and crowded tissues where cell segmentation is often ambiguous. We present Ultrack, a versatile and scalable cell tracking method that tackles this challenge by considering candidate segmentations derived from multiple algorithms and parameter sets.

Is Palivizumabe a Protective Factor for the Development of Asthma in Children? A Systematic Review With Metanalysis.

Introduction: Premature infants are at increased risk of developing chronic respiratory diseases, predisposing them to severe infections, such as those caused by respiratory syncytial virus (RSV). Palivizumab reduces the severity of RSV infections in high-risk children; however, its relationship with asthma development in premature infants remains unclear.

Objective: This systematic review with meta-analysis aimed to review the literature and assess whether prophylaxis with palivizumab protects premature infants without congenital heart disease from developing asthma.

6-Nitrodopamine Release From Mouse Seminal Vesicles Is Dependent on Endothelial Nitric Oxide Synthase (eNOS) Activation.

Human seminal vesicles present basal release of epithelium-derived 6-nitrodopamine (6-ND) and this catecholamine potentiates noradrenaline-induced contractions. Since nitric oxide synthase (NOS) activation is a determining factor involved in 6-ND biosynthesis, this study aimed to investigate which NOS isoform is responsible for the 6-ND release in mouse isolated seminal vesicles (MISV). Male control, eNOS, NOS, iNOS, and e/n/iNOS mice were used.